Human activities affect wildlife in several ways, ungulates tend to avoid areas of high human use and alter their behavior to avoid human activity. We used remote camera traps to quantify the relative abundance and activity of wild ungulates in high and low human use areas within Nagarjunasagar Srisailam Tiger Reserve (NSTR). Major human activity in NSTR included collection of forest produce and fuel wood, and livestock grazing. Poaching for bush-meat and the use of hunting dogs was also prevalent, but could not be quantified. The relative abundance of wild ungulates was high in low human-use areas except for chital and wild pigs, which require flat terrain and water found in prime areas for settlements. Diurnal ungulates like Chital and Nilgai substantially altered their activity in response to human activity, as did nocturnal species like Sambar and Mouse Deer. The demographic response of ungulates in NSTR has been poor compared to other tiger reserves that have been made free of human use. Our research highlights the importance of having human-free protected areas so as to achieve the desired conservation objectives of harbouring viable populations of large carnivores that require high prey abundance.