Application Of Mycorrhiza Research Articles

Influence of Ameliorant and Mycorrhiza Application on Growth Performance and Yield of Glutinous Maize

The application of ameliorants combined with mycorrhizal inoculants can enhance the fertility of sandy soil by improving its physical, chemical, and biological properties. This research aims to evaluate the influence of ameliorant and mycorrhiza application on growth performance and yield of glutinous maize. A randomized block design was employed, consisting of five treatments: F1: 75% cow manure + 25% mycorrhizal inoculant; F2: 75% compost + 25% mycorrhizal inoculant; F3: 75% 'Subur' organic fertilizer + 25% mycorrhizal inoculant; F4: 75% rice husk biochar + 25% mycorrhizal inoculant; and F5: a mixture of 20% cow manure, 20% compost, 20% 'Subur' organic fertilizer, 20% rice husk biochar, and 20% mycorrhizal inoculant. The results indicate that the use of a balanced organic ameliorant mixture with 20% mycorrhiza can significantly improve sandy soil fertility, as well as the growth and productivity of glutinous maize. The F5 treatment, which consists of 20% cow manure, 20% compost, 20% 'Subur' organic fertilizer, 20% rice husk biochar, and 20% mycorrhizal inoculant, is recommended as the best practice for cultivating glutinous maize on sandy soils.

The Mitigating Effects of Biostimulant Amendments on the Response of Purslane Plants Grown under Drought Stress Conditions

Portulaca oleracea L. is a wild edible plant with high potential for exploitation in commercial cropping systems due to its nutritional value and great adaptability to abiotic stress conditions. The present study aimed to investigate the response of purslane plants grown under drought stress conditions (100%, 80%, and 60% of field capacity (FC)) and the implementation of biostimulant amendments (control without amendment, plant growth-promoting rhizobacteria (PGPR), mycorrhiza, and effective microorganisms (EMs)) for two consecutive years. In the two-year experiment, the greatest height was recorded in plants grown under no-stress conditions and inoculated with PGPR. The highest branch number, and fresh and dry weight of aboveground and underground parts were observed under no-stress conditions at the mycorrhiza treatment. Moreover, mycorrhiza application in plants growing under 100% FC resulted in the highest N, P, total carbohydrates, and vitamin C and the lowest nitrate and proline contents in leaves. Purslane plants grown under 100% FC and inoculated with PGPR treatment resulted in the highest K and total chlorophyll leaf contents. Additionally, growing plants under mild drought stress (80% FC) combined with biostimulant application (e.g., inoculation with mycorrhiza, PGPR, and EM) may improve plant growth characteristics and mitigate negative stress effects. In general, the applied biostimulant amendments alleviated the adverse effects of drought on plant growth and leaf chemical composition indicating the importance of sustainable strategies to achieve high yield and sufficient quality within the climate change scenario.

Effect of bio-fertilizers and seaweed extract on growth and yield of wheat (Triticum aestivum L.) under different irrigation regimes: Two-year field study

Wheat productivity is constrained by genetic, agronomic, and climate factors, though it is an important crop for food production worldwide. The present study evaluated the effect of bio-fertilizer consortia and seaweed extracts on the growth and yield of two wheat varieties under different irrigation regimes in a field study. This experiment was conducted in a split-split plot based on a randomized complete block design with four replications in 2018 and 2019. Irrigation treatments were the main factor, wheat variety (Sardari and Sirvan) the sub-factor, and bio-fertilizers the sub-sub-factors. The results showed that irrigation regimes significantly improved leaf width, number of leaves, fresh weight of roots and shoots, osmotic potential, leaf water content, and number of stomata respectively by 57.53, 38.59, 106.65, 135.29, 87.92, 14.22 and 13.77, 88.02 and 96.11 percent compared to dry-land conditions. Applying one- and two-times irrigation increased grain yield by 51% and 79%, respectively, and the response varied in wheat varieties. Sardari variety due to having smaller leaf dimensions (Leaf length and width) and lower fresh and dry weight of roots and shoots, as well as lower leaf and tissue water content, had lower grain yield than the Sirvan variety. All the bio-fertilizers positively impacted the growth and yield of both varieties. However, the highest average grain yield in the first and second years of the experiment (with an average of 5226.25 and 4923.33 kg/ha, respectively) were found under the combined application of Mycorrhiza + Nitrozist and Phosphozist + Seaweed extract. The results of the present study underscore the importance of irrigation regimes and consortia of bio-fertilizers for improving grain yield. This study also highlighted the resilience of the studied wheat varieties and bio-fertilizers to projected climate changes. These findings could provide insights into adaptive strategies for mitigating the impact of climate change on wheat production.

The effect of mycorrhiza on the growth of Ylang-Ylang seedlings

Abstract Ylang-ylang (Cananga odorata forma genuina Steenis) is a plant with high economic value and many benefits. Mycorrhizal application is necessary to obtain a quality mother plant. Therefore, a study on the mycorrhiza effect on ylang-ylang growth was needed, especially in the seedling stage. This study aimed to analyse the impact of mycorrhiza infection on the development of ylang-ylang seedlings. The method used in this study was the isolation of Claroideoglomus etunicatum spores. Ten of each spore was inoculated onto the sterilised roots (using 5% Natrium hypochlorite) of the three-month-old ylang-ylang seedlings and then planted on zeolite media and soil in a 6 x 10 cm pottery, which was then transferred to each pot 13 cm in diameter and 16 cm in height. For comparison, zeolite and soil media were used without the addition of mycorrhiza. The result of this study showed that the application of mycorrhizae using soil media had the highest increase in height (4.19 cm), diameter (0.72 mm), number of leaves (2.6 strands), and number of buds (0.8 leaf buds) compared to other treatments.

Biostimulant and Arbuscular Mycorrhizae Application on Four Major Biomass Crops as the Base of Phytomanagement Strategies in Metal-Contaminated Soils.

Using contaminated land to grow lignocellulosic crops can deliver biomass and, in the long term, improve soil quality. Biostimulants and microorganisms are nowadays an innovative approach to define appropriate phytomanagement strategies to promote plant growth and metal uptake. This study evaluated biostimulants and mycorrhizae application on biomass production and phytoextraction potential of four lignocellulosic crops grown under two metal-contaminated soils. Two greenhouse pot trials were setup to evaluate two annual species (sorghum, hemp) in Italy and two perennial ones (miscanthus, switchgrass) in China, under mycorrhizae (M), root (B2) and foliar (B1) biostimulants treatments, based on humic substances and protein hydrolysates, respectively, applied both alone and in combination (MB1, MB2). MB2 increased the shoot dry weight (DW) yield in hemp (1.9 times more), sorghum (3.6 times more) and miscanthus (tripled) with additional positive effects on sorghum and miscanthus Zn and Cd accumulation, respectively, but no effects on hemp metal accumulation. No treatment promoted switchgrass shoot DW, but M enhanced Cd and Cr shoot concentrations (+84%, 1.6 times more, respectively) and the phytoextraction efficiency. Root biostimulants and mycorrhizae were demonstrated to be more efficient inputs than foliar biostimulants to enhance plant development and productivity in order to design effective phytomanagement strategies in metal-contaminated soil.

Optimization of polyamine and mycorrhiza in sorghum plant for removal of hazardous cadmium

Eco-friendly and sustainable practices must be followed while using the right plants and microbes to remove harmful heavy metals from the soil. The goal of the current study was to ascertain how effectively sorghum plants removed cadmium (Cd) from the soil using polyamines and mycorrhiza. Plant-biochemicals such as free amino acids, ascorbic acids, anthocyanin, proline, and catalase, APX, peroxidase activities were considered as markers in this study which revealed the adverse plant growth performance under 70 and 150 ppm of Cd concentration (w/w) after 30,60, and 90 days of treatment. The plants showed a mitigating effect against high Cd-concentration with exogenous use of mycorrhiza and putrescine. The treatment T17 (mycorrhiza +5 mM putrescine) showed a substantial decrease in the content of total free amino acid, ascorbic acid, catalase, APX, peroxidase by 228.36%, 39.79%, 59.06%, 182.79% 106.97%, respectively after 90 days as compared to T12 (150 ppm Cd). Anthocyanin content was negatively correlated (−0.503, −0.556, and −0.613) at p < 0.01 with other studied markers, with an increase by 10.52% in T17 treated plant as compared to T12. The concentration of Cd in root increased by 49.6% (141 ppm) and decreased in the shoot by 71% (17.8 ppm) in T17 treated plant as compared to T12 after 90 days. The application of mycorrhiza and putrescine significantly increased BCF (>1) and decreased TF (<1) for Cd translocation. The administration of mycorrhiza and putrescine boosted the Cd removal efficiency of sorghum plants, according to FTIR, XRD, and DSC analysis. As a result, this study demonstrates novel approaches for induced phytoremediation activity of plants via mycorrhiza and putrescine augmentation, which can be a promising option for efficient bioremediation in contaminated sites.

Effects of Mycorrhizal on The Growth and Yield of Cayenne Pepper (Capsicum frutescens L.)

Innovations are required to increase the production of cayenne pepper (Capsicum frutescens L), mainly due to the need for more quality land, such as dry land in Wamena. The innovation uses biological microorganisms that positively impact plants, such as arbuscular mycorrhizal fungi (AMF). Therefore, this experiment aimed to determine the response of the growth and yield of cayenne pepper plants cultivated in dry land to the application of mycorrhiza. The research was conducted using a completely randomized design with one factor: the mycorrhizae dose (0, 20, 50, 80, and 120 g). The research results were analyzed using ANOVA, and the analysis process continued with the 5% level DMRT test. The research results on the growth of cayenne pepper are visible in the plant height parameters of the 80 g treatment (75 DAP: 93.4 cm) and the 120 g treatment (95 DAP: 103.3 cm). Then, the stem diameter parameter showed a real influence by the 120 g mycorrhiza treatment at 35 and 75 DAP and the 80 g mycorrhiza treatment at 95 DAP. The excellent response of plants to the 80 g and 120 g mycorrhiza treatments also impacted the plant's total chlorophyll content and the number of leaves produced, which then impacted the number of productive branches. So, the effect on the yield of cayenne pepper plants is that the number of fruits treated with 80 g of mycorrhiza was 89.7 fruits, with the weight of the heaviest cayenne pepper fruit being 68.0 g by the treatment of 120 g of mycorrhiza with a fresh weight of 17.6 g and a total dry plant of 10.2 g compared to the control. Keywords: Arbuscular mycorrhiza, Biofertilizers, fungi, marginal land, Wamena

Growth and Essential Oil Production of Vetiver Plants Under Salinity Condition with Salicylic Acid and Mycorrhiza Application

Growth and Essential Oil Production of Vetiver Plants Under Salinity Condition with Salicylic Acid and Mycorrhiza Application

Early boosting of genetically improved Falcataria moluccana with mycorrhiza for better growth and disease tolerance

Falcataria moluccana is a widespread species that grows rapidly in Indonesia and produces timber used for carpentry. Nonetheless, this species is especially susceptible to gall rust disease. To find plants that can handle rust, a mix of breeding with progeny experiments and forest management methods, like arbuscular mycorrhiza (AM), were used. The point of this study is to find out if adding AM changes how F. moluccana seedlings grow and how they react to gall rust disease, both those that have been improved and those that have not. We will also look at the response by calculating the area under disease progress curves (AUDPC) value for rust disease to grow in F. moluccana seedlings. The experimental design comprised four different treatments, including varying quantities of mycorrhizae, as well as four different seed sources. For the mycorrhizal inoculation experiments, there was a control group and three treatment groups. The treatment groups got 5, 10, and 15 g of mycorrhiza per polybag, respectively. The seeds used in this study were sourced from two unimproved families, namely Java and Solomon, as well as two genetically improved families, F63 and F77. This study revealed that mycorrhizal application affects the growth of F. moluccana seedlings and significantly reduces both disease incidence and severity. The application of mycorrhiza reduces the disease incidence and severity; the AUPDC value of seedlings with mycorrhiza added is four times lower than that of seedlings without mycorrhiza. The disease severity was highest for the unimproved Solomon seedlings, despite the fact that the source of seeds had little impact on the disease incidence. Meanwhile, the genetically improved seedlings demonstrated lower disease incidence and severity rates than seedlings without improvement, with an AUPDC value nearly half that of the unimproved seedling. In terms of diameter and height, F. mollucana seedlings derived from various seed sources were remarkably diverse, and seedlings with improvements grew much better than seedlings without improvements. This study shows how arbuscular mycorrhiza affects how well improved and unimproved F. moluccana seedlings deal with gall rust disease.

The effects of the combination of mycorrhizae, vermicompost and humic acid applications on ornamental sunflower growth parameters

This study aimed to investigate the effect of the combination of mycorrhizal fungi, vermicompost, and humic acid application on ornamental sunflower growth. The study employed ornamental sunflower (Helianthus annuus L. cv. ‘Sunsantion’) as the plant material. Seedlings, acquired from a local ornamental production company at the four-true-leaf stage, were planted in plastic pots (2 L) containing peat perlite medium (1:1 v/v). The research incorporated three distinct doses of humic acid (10, 20, and 30 ml/L) and vermicompost (10, 20, and 30 ml/L). Humic acid (HA) and vermicompost (VC) were applied by soil drenching with 200 ml solution per pot. After one week, the seedlings were transferred into the pots. In all Mycorrhizae applications, 2 g/pot mycorrhizae inoculated during seedling transferred into the pots. Additionally, 20 ml of humic acid (HA20/M2) and vermicompost (VC20/M2) were combined with mycorrhiza application and applied to the plants. No application was made to the control group. Relative growth rate (RGR) was calculated from harvested samples of individuals from the same application group at 15 and 30 days after plants were transferred into the pots. The highest RGR obtained from HA20/M2 and vermicompost VC20/M2 treatments was 3.55 and 3.48, respectively. Additionally, the highest values of flower diameter (11.71 cm), stem length (41.13 cm), stem diameter (8.46 cm), and flower longevity (10.4 days) were observed in VC20/M2 treatment.

Effect of application of arbuscular mycorrhizal fungi on growth and yield of soybean in different agroecosystems

Soybean is a legume that can be cultivated in various types of agroecosystems such as lowlands, dry land, and swamps with various types of soil. This research aims to analyze the effect of mycorrhizal application on the growth and yield of soybeans planted in various agroecosystems. The research was designed with a two-factor randomized block design. Location of soybean planting as the first factor consists of four soybean development locations in Takalar Regency, South Sulawesi, namely: soybean cultivation in dry land, lowland in Galesong subdistrict, soybean cultivation in dryland, and lowland in South Galesong subdistrict. Application of mycorrhizal is the second factor which consists of two levels: no application, and mycorrhizae application. The results showed that the location of planting had a significant effect on soybean production, where soybean cultivation in lowlands resulted in significantly higher soybean production. The mycorrhizal application treatment obtained significantly higher soybean growth and production. The results of the analysis of soil chemical properties show that the total N content of soil in lowland land is moderate, P-available has low status, and low C-organic status. Galesong dry land showed a high total N content of 0.59%, but available P and organic C are very low.

Effect of Beneficial Microbes on Macronutrient Uptake and Oil Palm Seedling Growth in Peat Soil

Peat soil has low fertility due to low content of macronutrients, such as N, P, K, Ca, & Mg. Efforts that can be made to increase nutrient availability and nutrient uptake for plants on peatlands are the utilization of beneficial microbes such as endophytic bacteria and arbuscular mycorrhizae. This study aims to identify the role of endophytic bacteria and arbuscular mycorrhizae applied to peat soil media to help increase macronutrient uptake and the growth of oil palm seedlings (main nursery). This study was conducted in June – December 2022. The peat soil sample was collected from oil palm plantations located in Siak Regency, Riau, with coordinates 0°45′22″ N, 101°44′27″ E. The experimental design used in this study was a completely randomized design (CRD) with 8 treatments and 5 replicates. The treatment given in this study was a variation of beneficial microbes with zeolite as the carrier. Beneficial microbes used in this study were arbuscular mycorrhizae, namely Glomus sp, and endophytic bacteria, i.e. Burkholderia cenocepacia and Pseudomonas fluorescens. Parameters observed in this study were plant growth (every 1 month) and leaf nutrient (6 months after planted). The results of this study indicated that the consortium application of endophytic bacteria and arbuscular mycorrhizae was the best treatment which was shown to have the best growth of oil palm seedlings compared to all treatments with plant height increment of 55.5 cm; stem diameter increment of 34.3 mm; and the number of fronds addition as many as 11 fronds. This is also supported by macronutrients content in the leaves of oil palm seedlings such as N, P, and K.

POTENTIAL OF MYCORRHIZAL INOCULATION AND CATTLE RUMEN DIGESTA IN THE BIOREMEDIATION OF SPENT ENGINE OIL CONTAMINATED SOIL

Soil pollution by crude oil contamination has become a major constraint on agricultural productivity. Physicochemical techniques are often expensive. However, bioremediation of petroleum hydrocarbon polluted soil is cost-effective. Therefore, the study was carried out to analyze the influence of mycorrhiza and cattle rumen digesta on bioremediation of Spent Engine Oil (SEO) contaminated soil in Dutse, Jigawa state. Soil samples were randomly collected from the University Research and Teaching Farm. About 2.5 kg of sterilized topsoil (0–15 cm) was filled into pots and arranged in a 2×2×3 factorial experiment in completely randomized design with three replications. Mycorrhiza and cattle rumen digesta were at two levels, while SEO was at three levels. Data were collected on the total petroleum hydrocarbon (TPH) content, bacterial and fungal colony count. Data were analyzed using ANOVA at α 0.05. Results obtained from the study show that mycorrhiza and cattle rumen digesta increased the colonies of fungi and bacteria resulting in significantly enhanced TPH degradation in the contaminated soil. However, cattle rumen digesta significantly (p&lt;0.05) enhanced TPH degradation, bacterial and fungal population the most compared to mycorrhiza alone. Combined cattle rumen digesta and mycorrhiza application resulted in significantly (p&lt;0.05) lower residual TPH content in the contaminated soil compared to using cattle rumen digesta or mycorrhiza alone. Thus, cattle rumen digesta and mycorrhiza should be used in bioremediation of petroleum hydrocarbon impacted soils.

Nutrient distribution in various tissues of Licorice (Glycyrrhiza glabra L.) and the influence of soil fertility on the levels of its bioactive compounds

This study aimed to optimize the production of licorice (Glycyrrhiza glabra L.) in field conditions using two licorice ecotypes and various types of nutrient sources. Licorice yield, leaf and root nutrient content, and its bioactive compounds were assessed. Mycorrhiza inoculation resulted in the highest rhizome dry weight in the Lalehzar (101.3 g) and Kermanshah (395.6 g) ecotypes in the first and second years, respectively. Rhizome dry weight was higher than the roots in both ecotypes. Two years old Kermanshah ecotype exhibited the highest root dry weight by using urea. The harvest index increased in Lalehzar ecotype by application of vermicompost in the first year and cow manure coupled with plant growth-promoting bacteria (PGPB) in the second year. Nitrogen content in leaves and roots significantly increased in the Kermanshah ecotype using cow manure and PGPB. Application of mycorrhiza (in Lalehzar ecotype) and cow manure combined with urea (in Kermanshah ecotype) led to leaf phosphorus (P) content improvement. The highest root P content in Lalehzar and Kermanshah ecotypes achieved by using cow manure and urea, respectively. Fertilization by urea and mycorrhiza led to higher leaf potassium (K) content in Kermanshah and Lalehzar, respectively, while urea yielded the highest root K content in the Kermanshah ecotype. Root glycyrrhizic acid (GA) content peaked in the Kermanshah ecotype with cow manure and PGPB (138 and 372.8 mg g-1 DW in the first and second years, respectively). Rhizome GA content performed better in the Lalehzar ecotype using cow manure and PGPB (191.34 mg g-1 DW, first year) and in the Kermanshah ecotype with cow manure and urea (239.62 mg g-1 DW, second year). Fertilizers had not significant effect on glabridin (GL) content. Notably, GA and GL exhibited a negative correlation with potassium and nitrogen. This study highlights the importance of balanced nutrient management for licorice cultivation.

Response Of Growth And Production Of Shallot To The Genus Of Mycorrhiza And Npk Fertilizer

Mycorrhizal biofertilizer is a biotechnology agent and bio protector that is environmentally friendly and supports the concept of sustainable agriculture. The purpose of this study was to determine the effect of growth and production of shallots on the application of the genus Mycorrhizae and N,P,K fertilizers. This study used a completely randomized design method with two factors: The first factor was the application of the mycorrhizal genera Glomus (M1), Gigaspora (M2), Acaulospora (M3), and the Mycorrhizal Consortium (M4); and the second factor is 0-gram NPK fertilizer (NPK0), 25-gram NPK fertilizer (NPK25), 50-gram NPK fertilizer (NPK50), 75-gram NPK fertilizer (NPK75). The results of the identification of Glomus spore form is obovoid, the spore wall is more than one layer, yellow in color. Gigaspora spore form is globose, spore walls do not have an inner wall, and the spore is yellowish cream in color. Acaulospora spore is elliptic, has 2 spore walls, spores are yellow. The results of mycorrhizal responses and doses of NPK fertilizer on the growth and production of shallots were not significantly different according to the analysis of variance in all observation parameters, but some treatments consistently produced the highest results among other treatments. The highest treatment was M1.NPK50 produced an average number of shallots of 50 pieces, the number of shallot bulbs averaged 13.4 and the diameter of shallot bulbs averaged 4.9.

The effect of ascorbic acid and bio fertilizers on basil under drought stress.

Evaluate the effect of ascorbic acid application and coexistence of Mycorrhiza fungus and Azospirillium on basil (Ocimum basilicum L.) under drought stress. This experiment was performed as a split factorial in a randomized complete block design with three replications in the crop year 2017-2018 in Shahriar, Iran. In this experiment, irrigation was the main factor in three levels, including drought stress based on 40-70-100 mm from the evaporation pan of class A. Biofertilizer including growth-promoting bacteria (Azospirillium) and mycorrhiza fungus in four levels, including a(Non-consumption) B (Seeds of growth-promoting bacteria (Azospirillium)) C (Consumption of mycorrhiza fungus as seeds) D (Concomitant use of growth-promoting bacteria Azospirillium with mycorrhiza fungi as seeds) and ascorbic acid in two levels of foliar application, including A (Absence Application of ascorbic acid) and B (Application of ascorbic acid (two days after irrigation treatment)) was considered as a factorial factor. The results showed that the highest biological yield was obtained in drought stress of 40 mm and application of biological fertilizers in the form of mycorrhiza application with an average of 3307.1 kg/ha, which was about 70% more than 100 mm evaporation stress and no application of biological fertilizer. The use of ascorbic acid under drought stress conditions improved by 10%, the essential oil using ascorbic acid evaporated under drought stress conditions of 100 mm. As a general conclusion, the use of ascorbic acid and Mycorrhiza + Azospirillium biological fertilizer improved the quantitative and qualitative characteristics of basil under drought stress.

The role of mycorrhiza and humic acid on quantitative and qualitative traits of faba bean plant under different fertilizer regimes

In sustainable agricultural ecosystem management, a key focus is on minimizing chemical fertilizer use and maximizing the use of non-chemical alternatives. Our study was designed to explore the impact of mycorrhizal fungi and humic acid (HA) on the quantitative and qualitative characteristics of faba beans under varying chemical fertilizer levels. The experiment involved different treatments, which included HA and mycorrhiza in four variations (control, HA, HA+ mycorrhiza, and mycorrhiza alone), and nitrogen, phosphorus, and potassium (NPK) fertilizers at three different dosages (50%, 75%, and 100% of the recommended amount). The findings revealed that combining HA with mycorrhiza significantly influenced root colonization, with the highest chlorophyll a concentration (1.58 mg g⁻¹) observed in the HA+ mycorrhiza treatment at 75% NPK. This treatment also led to the highest counts in seeds per pod, number of pods, and weight of 100 seeds. Between the 100% and 75% NPK levels in the HA+ mycorrhiza treatment, no significant differences were noted in terms of grain and biological yield. The greatest grain yields were measured at 4356 kg ha⁻¹ and 4322 kg ha⁻¹ for the HA+ mycorrhiza treatment at 100% and 75% NPK, respectively. Additionally, the highest concentrations of Fe, N, P, K, and Zn were observed with the HA+ mycorrhiza application at the 100% NPK level.

Effects of mycorrhiza and phosphate fertilizers on the growth and yield of foxtail millet (Setaria italica L.) under drought stress conditions

Millet is a cereal plant that’s potential for rice substitution. This plant is adaptive to be cultivated in dry land but has a tolerance limit to drought stress. Mycorrhiza and phospate (P) fertilizer treatments help plants adapt to this condition. This study aimed to determine the effects of phosphate fertilizer and mycorrhiza as well as their interaction effects on the growth and yield of foxtail millet (Setaria italica L.) under drought stress. The experiment was carried out from January to June 2020 in the experimental farm, Faculty of Agriculture, University of Jenderal Soedirman. The research was arranged in a factorial randomized complete block design consisting of two factors. The first factor was the dose of SP-36 fertilizer per polybag, namely P0 = 0 g, P1 = 37,5 kg.ha⁻¹ (25%), P2 = 75 kg.ha⁻¹ = 0.88 g/polybag (50%), and P3 = 150 kg.ha⁻¹. The second factor was the dose of mycorrhiza biofertilizer, namely M0 = 0 g.polibag⁻¹, M1 = 33.3 g. polibag⁻¹ and M2 = 66.6 g.polybag⁻¹. The treatment was replicated three times. The data observed were analyzed using the F test, continued with DMRT test at p=0.05. The results showed that SP-36 fertilizer application at half of the recommended dose (0.88 g/polybag) could increase growth variables, such as leaf area, panicle length, and seed weight. Mycorrhizae application 33.3 g/polybag could improve variables such as plant height, leaf area, panicle length, and seed weight. It also accelerated the initiation of panicle emergence compared to control.

Application of mycorrhizae and rhizobacteria inoculations in the cultivation of processing tomato under water shortage

The effect of mycorrhizal fungi and plant growth promoting rhizobacteria on some physiological properties, yield and soluble solid content (Brix) of ‘Uno Rosso’ F1 processing tomato was studied under water scarcity. Inoculation was performed with mycorrhizal fungi (M) and rhizobacteria preparation (PH) at sowing (M1, PH1) and sowing + planting (M2, PH2). The treated and untreated plants were grown with regular irrigation (RI = ET100%), with deficit irrigation (DI = ET50%) and without irrigation (I0). In drought, the canopy temperature of plants inoculated with arbuscular mycorrhizal fungi (M1, M2) decreased significantly, however, the decrease was small in those treated with the bacterium (PH1, PH2), while the SPAD value of the leaves of plants treated only with Phylazonit increased significantly. On two occasions, inoculations (M2, PH2) significantly increased the total yield and marketable yield, however, under water deficiency, a higher rate of green yield was detected than untreated plants. In dry year using deficit irrigation, the one-time inoculation (M1, PH1) provided a more favorable Brix value, while the double treatments reduced the Brix. In moderate water scarcity, the use of mycorrhizal inoculation (M2) is preferable, while under weak water stress, the use of rhizobacteria inoculation (PH2) is more favorable.

Reforestation in The Reclamation Area of Pongkor Gold Mining

Ex-mining land is categorized as land with a high level of degradation. The loss of biodiversity, low soil fertility, loss of several layers of soil horizons, unbalanced texture, and even contamination can occur due to mining activities. Reforestation of mining land has been carried out with the approaches of improving the physical properties of the soil, selecting plant types, and improving soil biology in ex-gold mining land in Bogor. The addition of compost significantly increased organic matter content, C/N ratio, macronutrients (N, P, K) content, and improved soil texture. The application of Mycorrhiza and Rhizobium significantly increased the growth of forest plants such as trembesi (Samanea saman), gmelina (Anthocepalus cadamba), and ganitri (Elaeocarpus angustifolus) in ex-gold mining areas. Applying that significantly improved vegetation diversity on ex-gold mining land, including more than eight types of understory plants, and more than five plants were from the trees group. The result showed that reforestation of ex-mining land was feasible if it was carried out with a comprehensive approach.