Mycobacterium Triviale Research Articles

A Case Report on Concurrent Respiratory Symptoms and Axillary Lymphadenectomy Possibly caused by Mycobacterium Triviale

A Case Report on Concurrent Respiratory Symptoms and Axillary Lymphadenectomy Possibly caused by Mycobacterium Triviale

Isolation and identification of mycobacteria from captive reptiles

The occurrence of Mycobacterium species in clinically healthy pet reptiles was studied in Italy during the period 2004–2006. The feces samples of 223 animals were examined bacteriologically. Thirty-seven strains were isolated, in particular from 13/18 (72.2%) ophidians, 13/134 (9.7%) saurians and 11/71 (15.5%) chelonians. The isolates were classified, after HPLC analysis of bromophenacyl esters of cell wall mycolic acids, as Mycobacterium fortuitum (14 strains, 37.8%), Mycobacterium fortuitum-like (17, 45.9%), Mycobacterium peregrinum (4, 10.8%), and Mycobacterium chelonae (1, 2.7%). M. fortuitum was isolated from seven pythons, five saurians and two turtles; M. fortuitum-like from six saurians, six pythons and five turtles; M. peregrinum from four turtles; M. chelonae from one lizard. One isolate from an Iguana iguana could not be identified by HPLC analysis showing a previously unreported profile. Comparative 16S rDNA sequencing showed a low similarity with Mycobacterium triviale (97.2%) and Mycobacterium confluentis (97.1%). On the basis of such data the unidentified bacterium turned out to belong to a not yet described Mycobacterium species.

Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale

A novel slow-growing, non-chromogenic mycobacterium (strain 01-305(T)) was isolated from a patient with pulmonary dysfunction. Growth characteristics, acid-fastness and the results of 16S rRNA gene sequencing supported the placement of this strain within the genus Mycobacterium. Phenotypically, strain 01-305(T) was generally similar to Mycobacterium triviale ATCC 23292(T), but some unique biochemical characteristics were observed. The 16S rRNA gene sequence of strain 01-305(T) was similar to those of M. triviale ATCC 23290 (GenBank accession no. AY734996, 99.9 % similarity) and M. triviale ATCC 23291 (AY734995, 99.9 %); however, it differed substantially from that of M. triviale ATCC 23292(T) (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences placed strain 01-305(T) in the slow-growing Mycobacterium group close to M. triviale ATCC 23290 and M. triviale ATCC 23291, but not M. triviale ATCC 23292(T). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, and the hsp65 and rpoB genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 01-305(T) represents a novel mycobacterial species, for which the name Mycobacterium koreense sp. nov. is proposed. The type strain is 01-305(T) ( = DSM 45576(T) = KCTC 19819(T)).

Mycobacterium triviale Presenting as Endobronchial Lesion in a Young Immunocompetent Girl

The Mycobacterium terrae complex, consisting of three saprophytic species, (M. terrae, M. nonchromogenicum, and M. triviale), they rarely cause diseases in immunocompetent people. There are few case reports of lung involvement by Mycobacterium terrae complex. We reported a 13 years old immunompetent girl with a cavitary opacity seen on chest imaging and bronchoscopy revealed an endobronchial lesion, sputum and tissue specimens revealed growth of Mycobacterium triviale. Our patient is unique as it is the first case reported in Saudi Arabia with this type of Mycobacteria, in addition there is no case reported in the literature with Mycobacterium triviale with endobronchial lesion, the patient treated with a combination of rifampicin, ciprofloxacin and ethambutol for six months, she showed good response with resolution of radiological abnormalities and she did not have relapse after one year of follow up.

Relapsing Peritonitis Due to Co-Infection with Mycobacterium triviale and Candida albicans in a CAPD Patient

Relapsing Peritonitis Due to Co-Infection with <i>Mycobacterium triviale</i> and <i>Candida albicans</i> in a CAPD Patient

Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean

The identification of mycobacterial species in clinical isolates is essential for making patient care decisions. Polymerase chain reaction (PCR) restriction enzyme analysis (PRA) is a simple and rapid identification method, based on amplification of 441 bp of the hsp65 gene and restriction with BstEII and HaeIII. As a contribution to the validation of PRA, a multicenter study was performed in eight laboratories located in Argentina, Brazil, Colombia, Chile, and Guadeloupe. Each laboratory received 18 coded isolates from the collection of the Institute of Tropical Medicine (Antwerp, Belgium), representing duplicates of nine laboratory strains: Mycobacterium terrae CIPT 140320001, Mycobacterium scrofulaceum CIPT 140220031, Mycobacterium flavescens ATCC 14474, Mycobacterium triviale ATCC 23292, Mycobacterium nonchromogenicum ATCC 19530, Mycobacterium chitae ATCC 19627, Mycobacterium abscessus ATCC 19977, Mycobacterium kansasii ATCC 12478, and Mycobacterium peregrinum ATCC 14467. A detailed protocol including amplification, enzymatic digestion, and gel preparation was provided to each laboratory. Two laboratories identified correctly all 18 (100%) isolates, one identified correctly 17 (94.5%), two identified 14 (77.7%), one identified 11 (61%), and two identified 8 (44.4%) isolates. Errors detected in laboratories with more than 77% accuracy were associated with electrophoresis running conditions and an unspecific amplicon produced by a single strain. Lower accuracy was mainly related to inappropriate use of DNA markers and insufficient training in interpretation of patterns. In conclusion, the PRA method was readily implemented in some Latin American and Caribbean laboratories of mycobacteria, but improvements in critical points, as gel running conditions and training in interpretiation of patterns, are needed in order to improve accuracy. In others, improvement in critical points is still necessary.

Mycobacterium brumae sp. nov., a Rapidly Growing, Nonphotochromogenic Mycobacterium

Strains of a new species of rapidly growing, nonphotochromogenic mycobacteria, Mycobacterium brumae, have been isolated from water, soil, and human sputum samples in Barcelona, Spain. The inclusion of this organism in the genus Mycobacterium is based on its acid-alcohol fastness, its DNA G+C content, its mycolate pattern, and its mycolate pyrolysis esters. A study of 11 strains showed that they form a homogeneous group with an internal phenotypic similarity value of 94.9 ± 3.79%. The results of a comparison with 39 other mycobacterial species and subspecies are also presented. DNA relatedness studies showed that the M. brumae strains studied form a single DNA hybridization group which is less than 30% related to 15 other species of the genus Mycobacterium. Thin-layer chromatographic analysis showed that only α-mycolates are present. Unlike Mycobacterium fallax and Mycobacterium triviale α-mycolates, M. brumae α-mycolates release only 22-carbon atom esters after pyrolysis. Strain CR-270 is the type strain; a culture of this strain has been deposited in the Collection Nationale de Cultures de Microorganismes de l’Institut Pasteur, Paris, France, as strain CIP 103465.

Third Report of the Cooperative, Open-Ended Study of Slowly Growing Mycobacteria by the International Working Group on Mycobacterial Taxonomy

The open-ended study of the International Working Group on Mycobacterial Taxonomy is an ongoing project designed to characterize slowly growing strains of mycobacteria that do not belong to well-established or thoroughly characterized species. In this third report we describe numerical clusters that encompass the type strains of Mycobacterium szulgai, Mycobacterium triviale, Mycobacterium shimoidei, Mycobacterium asiaticum, Mycobacterium simiae, and Mycobacterium malmoense. Descriptions and discussion of the taxonomic status of three additional unnamed clusters, as well as a cluster that encompasses the erstwhile type strain of the presently invalid species “Mycobacterium paraffinicum,” are also presented.

Necessity of Differentiation of Mycobacterium Non-Chromogenicum From Mycobacterium Terrae and Mycobacterium Triviale

"Necessity of Differentiation of Mycobacterium Non-Chromogenicum From Mycobacterium Terrae and Mycobacterium Triviale." American Review of Respiratory Disease, 129(5), p. 883

Annual change of the isolation rates of mycobacteria from sputum specimens.

Microbiology and ImmunologyVolume 26, Issue 12 p. 1189-1193 NoteFree Access Annual Change of the Isolation Rates of Mycobacteria from Sputum Specimens Michio Tsukamura, Michio Tsukamura The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorShoji Mizuno, Shoji Mizuno The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorHaruo Toyama, Haruo Toyama The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorHiroshi Murata, Hiroshi Murata The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorYuji Fukaya, Yuji Fukaya The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorTokue Ohmi, Tokue Ohmi The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this author Michio Tsukamura, Michio Tsukamura The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorShoji Mizuno, Shoji Mizuno The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorHaruo Toyama, Haruo Toyama The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorHiroshi Murata, Hiroshi Murata The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorYuji Fukaya, Yuji Fukaya The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this authorTokue Ohmi, Tokue Ohmi The National Chubu Hospital, Obu, Aichi, 474Search for more papers by this author First published: December 1982 https://doi.org/10.1111/j.1348-0421.1982.tb00269.xCitations: 2AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL REFERENCES 1Awe, R. J., Gangadharam, P. R., and Jenkins, D. E. 1973. Clinical significance of Mycobacterium fortuitum infections in pulmonary disease. Am. Rev. Respir. Dis. 108: 1230– 1234. 2Cianciulli, F. D. 1974. The radish bacillus (Mycobacterium terrae): Saprophyte or pathogen? Am. Rev. Respir. Dis. 109: 138– 141. 3Dechairo, D. C., Kittredge, D., Myers, A., and Corrales, J. 1973. Septic arthritis due to Mycobacterium triviale. Am. Rev. Respir. Dis. 108: 1224– 1226. 4Edwards, M. S., Huber, T. W., and Baker, C. J. 1978. Mycobacterium terrae synovitis and oeteomyelitis. Am. Rev. Respir. Dis. 117: 161– 163. 5Hirota, S., and Tomiyama, T. 1976. A right foot joint infection with Mycobacterium nonchromogenicum. A case report. Kekkaku 51: 389– 392 (in Japanese). 6Kubala, E. 1972. Some aspects of disease caused by atypical mycobacteria. Scand. J. Respir. Dis. 80: 11– 16. 7Tsukamura, M. 1975. Identification of mycobacteria, p. 1– 75. The National Chubu Hospital, Obu, Aichi 474. 8Tsukamura, M. 1976. Parasitism and infection with ‘atypical’ mycobacteria in the respiratory organ. Kekkaku 51: 19– 23 (in Japanese with English summary and tables). 9Tsukamura, M. 1977. Infections due to Group VI mycobacteria (a review). Iryo 31: 1187– 1196 (in Japanese with English-summary and tables). 10Tsukamura, M. 1977. Source and infection route to humans of pathogenic mycobacteria other than tubercle bacilli. Kekkaku 52: 261– 267 (in Japanese with English-summary and tables). 11Tsukamura, M., Mizuno, S., Murata, H., Nemoto, H., and Yugi, H. 1974. A comparative study of mycobacteria from patients' room dusts and from sputa of tuberculous patients. Source of pathogenic mycobacteria occurring in the sputa of patients as casual isolates. Jpn. J. Microbiol. 18: 271– 277. 12Tsukamura, M., and Tsukamura, S. 1964. Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis by p-nitrobenzoic acid susceptibility. Tubercle 45: 64– 65. Citing Literature Volume26, Issue12December 1982Pages 1189-1193 ReferencesRelatedInformation

Hydroxylamine sensitivity and salt tolerance in screening and identifying mycobacteria

Hydroxylamine sensitivity offered no advantage over p-nitrobenzoicacid in distinguishing between tubercle bacilli and other (opportunist and non-significant) mycobacteria. It was of limited use in identification procedures. Salt tolerance assisted in the identification of strains which may be Mycobacterium triviale and M. chelonei var borstelense.

Artificial Induction of an Organism Similar to Mycobacterium triviale from Mycobacterium gordonae by Successive Ultraviolet Irradiations

An organism phenetically indistinguishable from Mycobacterium triviale was isolated from Mycobacterium gordonae (ATCC 14470) by successive ultraviolet irradiations after three selection steps. From the parent (a smooth-colony-forming, scotochromogenic strain) were isolated: (i) photochromogenic mutants; (ii) rough-colony-forming mutants; and (iii) non-photochromogenic (pigmentless) mutants. In the third step of mutation, some mutants showed simultaneous changes of other characters, e.g., loss of the ability to utilize several carbon sources. After ultraviolet irradiation, mutant colonies comprised 1 to 2 per 103 surviving colonies. It was impossible to isolate non-photochromogenic mutants directly from parent scotochromogenic bacteria. Mutants could be isolated only from photochromogenic mutants. This is in marked contrast to a previous work in which non-photochromogenic mutants were isolated directly from parent, scotochromogenic Mycobacterium scrofulaceum.

Phagocytosis of heat-killed radiolabelled mycobacteria in human mononuclear phagocytes cultured in vitro.

Human mononuclear phagocytes cultured in vitro for 8 dyas were exposed to 125I-labelled, heat-killed Mycobacterium triviale. The microorganisms were apparently engulfed, but no digestion occurred within a period of 16 days after the engulfment, measured as release of radioactivity to the medium and observed microscopically. Attempts were made to stimulate intracellular digestion of the bacteria. Pre-incubation with BCG-stimulated lymphocytes or with supernatants from BCG-stimulated lymphocyte cultures did not increase the digestive ability of the cells. However, pre-incubation with BCG-stimulated lymphocytes or with supernatants caused detachment of the cells during the following digestion period, probably due to a cytotoxic effect of autologous, transformed lymphocytes on macrophages. When the macrophages were cultured in the presence of autologous lymphocytes and BCG, a similar effect was found.

Acute Infantile Septic Arthritis of the Hip Due to Mycobacterium triviale

Acute Infantile Septic Arthritis of the Hip Due to Mycobacterium triviale

Septic arthritis due to Mycobacterium triviale.

Mycobacterium triviale was the only organism cultured from the synovial fluid of an infant with septic arthritis. Supportive evidence for infection by this organism was the presence of Langhans’ gi...

Differential identification of mycobacteria VI. Mycobacterium triviale Kubica sp. nov

A new species of nonphot ochromogen mycobacteria is described. Most of the strains examined have been isolated from man; however, because of their lack of relationship to human disease, the name Mycobacterium triviale Kubica is proposed. The physical and biochemical characteristics, serologic activity, and thin layer chromatographic pattern of this new species serve to distinguish it from other nonphotochromo-genic mycobacteria in Runyon’s Group III.