Mussel Aquaculture Research Articles

Assessing the use of remote underwater video (RUV) to identify fish and their feeding behaviour in New Zealand's Greenshell™ mussel (Perna canaliculus) farms

Fish predation poses a significant and escalating challenge for the global mussel aquaculture industry. In New Zealand, Greenshell™ mussel farms experience crop losses of up to 100 % that are anecdotally attributed to fish predation, although the fish species responsible have not been confirmed. In this study, remote underwater video (RUV) was used to observe fish activity within four mussel farms at different stages of production in the Firth of Thames, New Zealand. The RUV enabled the identification of the fish species inhabiting mussel farms, including those responsible for predating cultured mussels. Four fish species were identified as frequent inhabitants of the mussel farms, with Australasian snapper (Chrysophrys auratus) and parore (Girella tricuspidata) the most abundant at three out of four mussel farm sites. Australasian snapper was the most common predator, taking as many as 2880 bites of the dropper lines holding mussels in a single 11 min 47 s video recording. Snapper formed feeding aggregations around dropper lines holding juvenile mussels (> 20 mm SL), indicating their potential to remove mussels from nursery farms in a short amount of time. Observations of the feeding behaviour of parore indicated their potential to contribute to the losses of mussels on recently seeded spat farms with these fish recorded tearing apart seeded lines, possibly targeting the macroalgae that is seeded out with the mussel spat. Besides confirming the identity and the predatory behaviour of fish species that are contributing to crop losses from mussel farms in New Zealand, this study also confirms the effectiveness of RUV methods for this purpose. This knowledge can be used to begin to develop mitigation strategies aimed at reducing crop losses in mussel aquaculture.

Use of Environmental DNA to Evaluate the Spatial Distribution of False Kelpfish (Sebastiscus marmoratus) in Nearshore Areas of Gouqi Island

This study aims to explore the spatial distribution of false kelpfish (Sebastiscus marmoratus) in the mussel farming area, artificial reef areas of Gouqi Island (Shengsi, China), and natural areas using eDNA detection methods. Surface and bottom water samples were collected at 12 stations in November 2022 and April 2023, totaling 52 samples. We used species-specific primers and probes for quantitative PCR (qPCR) detection of Sebastiscus marmoratus eDNA. The eDNA concentrations differed seasonally (p < 0.05) and did not differ (p > 0.05) among the three sampling areas and two water layers. The greatest eDNA concentrations occurred in the surface layer during the spring. Higher concentrations of Sebastiscus marmoratus eDNA were also found in the mussel aquaculture area. Temperature exhibited a significant positive correlation with Sebastiscus marmoratus eDNA concentration (p < 0.05). Additionally, we developed linear equations predicting the relationship between environmental factors and environmental factors, providing a reference for future fishery resource surveys.

The role of substrata width and millimeter scale surface micro-structure in the attachment of juvenile mussels

The global mussel aquaculture industry is constrained by the unreliable supply of wild seed mussels and high losses of seed mussels shortly after they are seeded onto coastal farms. The success in the collection of the settling larvae of mussel in the wild and the subsequent on-growing of early seed mussels in aquaculture are largely determined by the settlement and attachment behaviour of the larval and juvenile mussels, which is strongly influenced by the physical structure of their attachment substrata, especially filamentous substrata. This study aimed to identify an ideal set of morphological characteristics of filamentous substrata that would promote the improved attachment of early mussels and contribute to reducing the shortage of seed mussels in the New Zealand green-lipped mussel (Perna canaliculus) aquaculture industry. Artificial filamentous substrata with varying branch width and textured surfaces were tested for their ability to promote the attachment of wild juvenile mussels. The attachment of early juvenile mussels to 1.6 mm wide filamentous substrate was six times higher than their wider counterparts (i.e., 3.7, 5.6, 7.4, and 9.5 mm). There was also a higher proportion of small-size mussels (<0.99 mm shell length) on the 1.6 mm substrata. Furthermore, the filamentous substrata with regularly ridged surface contours (1 mm spacings) tended to attract more juvenile mussels. However, this preference was not consistent for small-size mussels on filaments of thinner width (i.e., 0.4, 0.8, 1.6, and 3.7 mm). Overall, the dimensions of filamentous substrates identified through this study will be valuable for improving the design of artificial substrata used in mussel aquaculture for improving its effectiveness for the attachment of seed mussels.

Genetic homogeneity and weak signatures of local adaptation in the marine mussel Mytilus chilensis

The natural populations of the marine mussel Mytilus chilensis and the associated aquaculture industry forms a sensitive social-ecological system that relies on the released propagules for cultivation in the highly heterogeneous environment (temperature, productivity, and salinity) of northern Patagonia (42–44 °S). We assessed spatial genetic structure, signals of local adaptation, and population assignment of M. chilensis analyzing 5963 SNPs from 125 individuals across six natural populations sampled over two consecutive years along the southeast Pacific coast (39° 25′ to 43° 07′ S, ~ 430 km). Neutral and putatively adaptive loci revealed high genetic diversity and low genetic differentiation among populations. Of the whole dataset, less than 1% (50) of loci were identified as putatively adaptive through multiple approaches, with only 0.1% detected in by all of them, and only two loci of them were correlated with environmental variables. No evidence of Isolation by Environment (IBE) was found, albeit a slight differentiation in the southern sampling location (Yaldad). These results suggest that the genetic structure observed is primarily shaped by neutral processes with weak signals of local adaptation. Gene-flow appears to be the main evolutionary force influencing the species’ population genetic structure. Because of the importance for the industry, the probability of correct assignment of individuals to their population of origin using allelic frequencies was evaluated. Analyses exhibited relatively low probabilities (< 50% for four out of six sites) of accurately assigning individuals to their geographic origin, with a limited success of SNP markers the for such purposes. Likely, species' high dispersal capacity, seed translocation, and the spill-over effect of mussel aquaculture prevents population genetic differentiation through high effective gene flow, hindering local genetic adaptation.

Interactive impact of residual pyrethroid compounds used in the Chilean salmon farming industry and coastal acidification conditions on the feeding performance of farmed mussels in northern Patagonia

The use of pyrethroids in aquaculture has been an important component of achieving a thriving salmon farming industry in Chile. While the residual presence of such substances is known to depend on environmental conditions, most ecotoxicological studies to date have not considered environmental context. Here, we conducted oceanographic monitoring combined with experiments aiming to estimate the effects of two pyrethroids on the feeding rates of larvae of farmed mussels, Mytilus chilensis. In additional experiments, mussel spats were exposed to both pyrethroids, but under contrasting temperature/pH so as to mimic winter and summer conditions. Experiments mimicking spring conditions revealed that both pyrethroid substances affected the feeding of mussel larvae as a function of concentration. Conversely, significant impact of pyrethroids on adults were not observed with regard to temperature and pH, but a significant impact of low temperature/low pH condition on ingestion rates was confirmed. Given the current status of increasing ocean acidification, the results of this study are expected to provide useful information with regard to achieving sustainable mussel aquaculture, especially considering both activities occur in similar geographic areas, and the expansion of salmon farming areas is ongoing in Chile.

Energy reserves and gut microbiota of marine mussels under combined exposure to pathogens and predation risk

In recent years, environmental issues such as eutrophication have led to proliferation of pathogenic bacteria and deterioration of aquaculture environmental water quality. However, the impact of pathogenic bacteria on the mussel-predator food chain in the marine environment is not yet fully understood. In this experiment, mussels were cultured with crabs (Charybdis japonica) for 14 days. Then crabs were retained or removed and pathogenic bacteria (Vibrio alginolyticus) was added and mussels were cultured for 3 days. C. japonica and V. alginolyticus were used as two stressors to explore their effects on energy content and gut microbiota of Mytilus coruscus. The metabolic parameters (protein, carbohydrate and lipid contents) of the gills and digestive glands, and the gut microbiota of the mussels were measured. The study found that presence of predation risk significantly increased the content of protein but decreased carbohydrate and lipid contents in the gills at 14th day. After 17 days of exposure, the presence of predation risk significantly reduced protein content but significantly increased lipid content in gills of mussel. The protein content in digestive gland was significantly increased, but the lipid content was significantly decreased. The presence of predation risk and pathogens had interactive effect on the protein content in the digestive glands of mussels. The presence of V. alginolyticus and predation risk affected the community structure, species richness, and diversity of gut microbiota. The most abundant phyla noted were Bacteroidota, Proteobacteria, and Firmicutes, and the most abundant genus noted were Prevotella, Klebsiella and Dialister. Alpha diversity analysis and beta diversity analysis revealed that the presence of V. alginolyticus and predation risk altered the microbial community structure. V. alginolyticus plays a positive role in the harmful microbiota of gut microbiota. The proportion of Bacteroidota and Proteobacteria in intestinal bacteria increased significantly. Linear discriminant analysis effect size (LEfSe) showed that the biomarkers in each group are significantly different at the genus level. Significant correlations between metabolic parameters (protein content and carbohydrate content) and the level of KEGG function prediction (amino acid metabolism and carbohydrate metabolism) were observed in gills and digestive glands. This study highlights the priority of energy responses in mussels under dual predator-pathogen pressure, and also highlights the complexity of predator-pathogen interactions. A better understanding of their impact on the gut microbiota and health of marine mussels will help support the sustainability of mussel aquaculture production.

Penguatan Permodalan Koperasi dengan Skema Crowdfunding pada Koperasi ISM Karya Sinar Bahari, Desa Tanggul Jaya, Kelurahan Banten, Kecamatan Kasemen, Kota Serang, Provinsi Banten

The ISM Karya Sinar Bahari Cooperative, located in Tanggul Jaya Village, Banten, faces significant challenges in accessing adequate capital to sustain its green mussel aquaculture operations. This community service project explores the implementation of crowdfunding as an innovative solution to strengthen the cooperative's capital base. Through this initiative, cooperative members received intensive training on crowdfunding-based capital management. The study identifies the potential of crowdfunding to enhance the cooperative's financial capacity and assesses its impact on improving member welfare and the success of the aquaculture business. The findings demonstrate that this scheme successfully engaged member participation and diversified the cooperative's funding sources.

Development of SPEEK-Coated IrOx Sensor for High-Biomass Aquatic pH Monitoring.

Instability is a key challenge for current pH sensors in practical applications, especially in aquatic environments with high biomass and redox substances. Herein, we present a novel approach that uses a highly stable IrOx sensing layer enveloped in a composite film of SPEEK doped with a silicon-stabilized ionic liquid (SP-IrOx). This design mitigates drift due to sensitive layer variations and minimizes interference from complex external conditions. After exhibiting robustness under moderately reducing conditions caused by S2-, I-, and ascorbic acid, the SP-IrOx sensor's efficacy was validated through real-time pH measurements in demanding aquatic settings. These included laboratory algal culture medium, sediment substrates, and mussel aquaculture areas. The sensor sustained accuracy and stability over extended periods of 6-8 days when compared to calibrated commercial electrodes. The deviations from reference samples were minimal, with a variance of no more than 0.03 pH units in mussel aquaculture areas (n = 17) and 0.07 pH units in an algal culture medium (n = 37). As a potentiometric, this solid-state electrode features a compact structure and low energy consumption, making it an economical and low-maintenance solution for precise pH monitoring in diverse challenging environments with high biomass and turbidity.

The declining availability of wild mussel seed for aquaculture in a coastal upwelling system

A general decline in foundation species at the rocky intertidal has been observed during the last decades all around the world and primarily related to climate change. In agreement with that trend, the mussel aquaculture sector in Galicia (NW Spain), the main production area in Europe, has warned over the last years about a decline in the availability of wild mussel seed from the rocky coast. Here we compile for the first time, mussel seed collection reports by mussel farmers in Galicia for the period 2006–2021. We employed that dataset as a proxy of mussel recruitment evolution in the rocky shore for the last 16 years. Temporal analysis of our data confirmed the reported decline (-148 t yr-1), particularly pronounced from 2012 onwards. The data base also allowed us to analyze inter-annual variability according to both, climatic variations and management scenarios. Since cultivated mussels conform a meta-population with wild mussels from the rocky shore, alterations on the market preferences towards smaller individuals at harvest, could also contribute to a reduction in reproductive output. Our results show a decrease in life-time egg production under certain scenarios. Nonetheless, coastal upwelling seems to be the largest factor conditioning recruitment abundance, explaining as much as 60% of the variability observed. Decline on recruitment abundance was highly modulated by the observed increment in frequency of intense upwelling events, exceeding 500 m3 km-1 s-1 between July and November. Meridional winds also determined the spatial recruitment patterns, pointing to the large role of wind forcing on mussel larval dispersal. Our results highlights how alterations on upwelling regimes related to climate change can interact with mussel population dynamics and also condition aquaculture sustainability and food security.

Impacts of seeding density on the abundance and size of juvenile mussels and biofouling accumulation in Greenshell™ mussel aquaculture

Growth of the Greenshell™ mussel (Perna canaliculus) industry, New Zealand's most significant aquaculture sector, is limited by spat abundance in the early stages of the aquaculture cycle. However, there are few management techniques to improve spat production. Here, we tested the effects of seeding density (low: 257, mid: 515, and high: 1030 mussels per 10 cm of culture rope) – a putative method to manage spat seeding efficiency and yield – on the abundance and size of P. canaliculus spat at four depths at two sites in the Marlborough Sounds. We also recorded the abundance and size of blue mussels, Mytilus galloprovincialis, which are a problematic biofouling species, as well as the biomass and composition of the biofouling accumulating on mussel culture ropes under the same experimental treatments. Although the abundance of P. canaliculus was greatest at the highest seeding density (∼200 mussels per 10 cm of rope at 87 days), this seeding density also experienced the greatest losses. However, while fewer spat were lost from the low-density seeding treatment, the overall number of spat per 10 cm was significantly reduced, resulting in few operational gains in terms of biofouling reduction or spat size. Overall, the seeding density of suggesting that seeding density of ∼1030 spat per 10 cm of rope was the optimal density tested in this study and could be a useful target density for mussel farmers.

Assessing the mechanical properties of biobased versus fossil-based ropes and their impact on the productivity and quality of mussel (Mytilus galloprovincialis) in longline aquaculture: Toward decarbonization

The expansion of offshore mussel aquaculture is intertwined with the rising demand for mussel grow-out ropes. Currently, these ropes are manufactured using fossil-fuel based or fossil-based plastics. In response to concerns about plastic pollution and sustainable aquaculture practices, biobased materials have emerged as more sustainable alternatives. Yet, the importance of the choice of raw materials, as well as the methods used in their production and sourcing, is key in determining the performance of materials in sea conditions within the context of aquaculture. This study evaluated the functionality of biobased ropes in terms of productivity and durability, comparing them to fossil-based counterparts during a one-year mussel culture in offshore conditions. Across one-year culture, noteworthy mussel losses were observed in the fossil-based (65%) ropes compared to the biobased B1 (53%) and B2 (18%) prototype ropes, B2 and B1 ropes yielding 85% and 23% more mussel (kg/ m rope) than the fossil-based ropes. Moreover, the higher correlations of shell length and body weight over time suggesting that biobased ropes may be more suitable substrates for mussel growth compared to fossil-based ropes. The structural distinction between the round and more uniform fine multifilaments in biobased ropes, as opposed to the clustered structure of flat fibres in fossil-based ropes, may have contributed to a more effective surface area for the initial attachment and growth of seeded mussels, avoiding subsequent losses. The use of biobased ropes contributed positively to the interplay between substrate structure, biofouling, and mussel attachment, which may be used for guiding further designs of aquaculture biobased gears. Despite seasonal variations, the final condition, proximate and fatty acid composition of mussel indicated their marketability and high nutritional quality. Higher shell length and mussel yields observed, indicating a potential for mussels cultivated on biobased ropes, especially in B2, to reach a higher market value. Regarding durability, linear density of biobased ropes remained unaltered after one-year offshore mussel culture. The biobased B2 rope prototype showed the highest ratio between the retention of mechanical properties (Load at Break and elongation) and the total mussel weight held for one year. The findings of this study validate the biobased B2 prototype as the most promising alternative to fossil-based ropes. It significantly boosted production yields of high-quality mussels while maintaining rope durability throughout one year in offshore culture. These results hold promise for reducing the reliance on fossil-based plastic ropes in mussel aquaculture, thereby contributing to decarbonization efforts.

The aggregation effect of offshore mussel farming on pelagic fishes

AbstractThe increase in hard artificial structures in the marine environment is contributing to ocean sprawl. As well as adding hard structure to the seabed, mussel aquaculture adds rope droppers and anchor lines to the water column, where such structure would otherwise be absent. This paper aims to monitor the effects of mussel headlines on the pelagic fish community over time, as well as to investigate whether there is an interaction between the age of cultivated mussels with the number of epibiota species and the abundance of pelagic fishes. Two surveys were carried out: a time series monitoring survey to assess whether the mussel headlines change the surrounding fish species relative to control areas over time, and a fish aggregation device effect survey to investigate whether older and larger mussels support greater abundances of pelagic fishes and epibiota. The most abundant fish species was Atlantic horse mackerel, Trachurus trachurus, with significantly greater numbers recorded around ropes growing older mussels. Results from the surveys show that the physical farm infrastructure has provided a substrate for colonisation by a variety of epibiota: A total of 21 species were recorded growing on the mussel ropes. Furthermore, the older and larger the mussels become, the greater the numbers of epibiota taxa that can colonise, coupled with a greater abundance of pelagic fish recorded around the ropes. The sustainable development of offshore Blue Industries is essential to produce renewable resources. This study brings essential evidence for marine managers and regulators that offshore mussel farming can provide benefits for pelagic and benthic species of both conservation and commercial importance while producing sustainable protein to feed a growing population.

Assessing the impact of an offshore longline mussel farm on local water circulation in a highly hydrodynamic energetic bay

Thought to be a sustainable choice, molluscs are the most consumed aquaculture foods after finfish. The expansion of the mussel aquaculture industry offshore reports lower environmental impacts compared to inshore farms. Although an offshore location has the potential to reduce a farm's ecological impacts, the effect of large developments on water currents is still not fully understood. High hydrodynamic regimes can influence the dispersion of farm biodeposits, organic loading, flow alterations with the potential to impact water residence time, particle and sediment dispersal (including larvae and biodeposits) and seabed sediment resuspension, which in turn, can have ecological impacts. Farm-induced flow changes of the UK's first large scale suspended longline mussel farm were assessed by a combination of oceanographic mooring and vessel-mounted Acoustic Doppler Current Profiler (ADCP) measurements. Data was separated by upstream and downstream according to the direction of the flow in relation to the farm. M-ADCP results showed a 28% decrease current velocities within the farm's boundaries, demonstrating within-farm current attenuation produced by mussel ropes drag. Flow was then redistributed above and beneath the farm showing velocity increases of 66% and 7% respectively, resulting in above-farm flow acceleration and downwelling (with up to 171% increase in near-seabed velocities compared to sea surface velocities). However, overall mean downstream sea surface velocities decreased by 63%. An overall 21% increase in near-seabed velocities showed the farm's effect on near-bed currents, the opposite to what is naturally achieved by seabed friction. This was further measured by VM-ADCP results also showing secondary flow acceleration at the farm's flanks and increased near-headline flow perturbations of up to 80% mean u velocities over a small horizontal scale (0.5 km), further demonstrating the effects of the farm on the local circulation. Through-farm surface current velocities (waves) were reduced by up to 72%. Flow changes were localised and dependent on the different tidal phases, the farm's design and, the abundance of mussel ropes providing drag.

Differential responses of selectively bred mussels (Perna canaliculus) to heat stress-survival, immunology, gene expression and microbiome diversity.

New Zealand's green-lipped mussel (Perna canaliculus) is an ecologically and economically important species. Marine heatwaves are increasing in frequency around NZ's coastline, and these events are correlated with increased stress and mortality of some aquaculture species. This study aimed to identify general biomarkers of heat stress in P. canaliculus and to assess whether responses differed between genetically distinct selectively bred mussels. We exposed three families of selectively bred mussels (families A, B and C) to three seawater temperature regimes in the laboratory: 1) a "control" treatment (ambient 12°C), 2) a 26°C heat challenge with a subsequent recovery period, and 3) a sustained 26°C heat challenge with no recovery. We investigated whether the survival, immune response (hemocyte concentration and viability, oxidative stress and total antioxidant capacity), hemocyte gene expression and gill microbiome differed between the families during the temperature challenges. In the sustained heat-stress treatment, family A had the highest survival rate (42% compared with 25% and 5% for families C and B, respectively). Gene expression levels significantly shifted during thermal stress and differed between families, with family A more dissimilar than families B and C. Family C had substantially more genes impacted by temperature treatment and timepoint than the other families, while family B had very little genes/pathways that responded to thermal stress. Genes related to heat shock proteins and immune responses (e.g., AIF1, CTSC, TOLL8, CASP9, FNTA, AHCY, CRYAB, PPIF) were upregulated in all families during heat stress. Microbiome species-richness differed between families before and during heat-stress, with family A having a distinctly different microbiome flora than the other families. Microbial diversity changed similarly in all families exposed to prolonged heat-stress, with species of Vibrio and Campylobacter increasing in these mussels. Our study highlights the use of non-lethal sampling of hemocytes as a diagnostic tool to explore the immune response and gene expression of selectively bred mussels, to predict their response to ocean warming. This approach can identify potential thermotolerant candidates for further selective breeding, which may increase the resilience of the mussel aquaculture industry in a warming ocean.

Short communication: Forecasting Shellfish Aquaculture Threats. An Earth Observation Derived Tool Aiming at Avoiding Microbiological Public Health Hazards

Shellfish aquaculture is of great importance in offering a sustainable food source, thus playing a major role towards achieving food security and nutrition, employment, and economic development in coastal areas. Mussel aquaculture, in particular, offers a substantial development opportunity for Black Sea riparian countries; however, a significant attention must be given to zoo-sanitary conditions and public health, considering the filter-feeding behaviour of these organisms. With the involvement of GFCM’s Aquaculture Demonstrative Center (hosted by NIMRD), the microbiological classification of bivalve mollusk production and relaying areas in Romania was performed. Yet, the regular monitoring of these areas is not enough to prevent accidental contamination of shellfish farms, in case a potential harmful discharge from a wastewater treatment plant occurs. In this contet, the goal of our research was to develop a prediction tool, focused on an early warning system of any possibly microbiologically loaded water discharge (Escherichia coli), using a downscaling of CMEMS Earth Observation data and in situ validation. The developed service module can give farmers the ability to predict a potential harmful outbreak and decide to harvest earlier or later and/or keep the mussels in a purification tank before marketing, in order to avoid any possible E. coli contaminated harvest to be put on the market involuntarily.

Distribution Analysis of Green Mussels (Perna viridis) in Banyuurip Village, Ujung Pangkah District, Gresik Regency

The aim of the research was to determine the Distribution analysis of Perna viridis diversity based on the suitability map for green mussel cultivation, analysis of community perceptions and the condition of water bodies. Perna viridis aquaculture is suitable for the waters of Banyuurip village based on the variables of sea depth, sea surface temperature, chlorophyll a concentration, and substrate radiance data. Heavy metal content of Pb is located and distributed in polluted areas. It was found that the potential for green mussel aquaculture is greater in warm water habitats, i.e. closer to the coast. In addition, the northeast was identified as a suitable habitat for green mussels. The mid-ocean area in the north-west is of low suitability due to the lack of substrate and chlorophyll. Analysis of community attitudes and behaviour showed that people remain enthusiastic about green mussel farming, although green mussel shell waste remains an environmental problem. Heavy metal analysis of green mussel samples showed that green mussels are still safe for consumption. Therefore, the main conclusion is that the habitat of Perna viridis in the waters of Banyuurip Village still has potential for cultivation, but the waters must be free from pollution.

Validation of a debiasing addition to Analytical Hieratical Process tools to increase the effects of side-stream utilization and the choice of the filtration system in the European mussel aquaculture and processing industry

Introduction: New EU regulations for increased sustainability and better utilization of side-streams are pushing the European mussel farming and processing industry to adapt to new regulations regarding their wastewater production, which leads to incentives for optimizing methods for better utilization of this side-stream resource. In this study, one such European company is faced with a choice of a filtration system out of three possible options, to invest in and achieve the option with the best balance between economic gain and environmentally sound production.Method: For this, a specialized Analytical Hieratical Processes (AHP) tool was utilized together with a supplementary Debias Your Decisions (DYD) tool to visualize the local best choice for the company.Results: Based on the generated user weights from the company it was apparent that the user weights were biased towards certain criteria in the AHP tool, which meant that half of the tool’s criteria had greatly diminished influence on the tool’s scores. This brought into question the objectivity of the Performance index (Pi) score results from the AHP tool when the user weights were applied, since these Pi scores deviated significantly from the expert Pi scores. The supplement tool DYD was found to be able to identify the dominant decision biases influencing the user weights in the current case, which enabled the implementation of relevant debiasing techniques to correct for the overweighted user weights from the company. With these corrections and the case company’s input data, the optimum alternative suggested for the case company is membrane filtration based on the final Pi scores for all weight sets.Discussion: This process and final debiased results further validated the AHP tools results as objectively grounded due to their convergence with the experts’ scoring results. This study also served as further validation for the new supplement DYD tool as the current sensitivity analysis showed the tool’s robustness to be high.

Transcriptome analysis reveals tissue-specific responses of Mytilus unguiculatus to Vibrio alginolyticus infection

Mytilus unguiculatus is an important economic bivalve species with wide consumption and aquaculture value. Disease is one of the primary limiting factors in mussel aquaculture, thus understanding the response of different tissues of M. unguiculatus to pathogens is crucial for disease prevention and control. In this study, we investigated the physiological and transcriptomic responses of the gills, adductor muscle, and mantle of M. unguiculatus infected with Vibrio alginolyticus. The results showed that V. alginolyticus infection caused inflammation and tissue structure changes in the gill, adductor muscle and mantle of M. unguiculatus. Meanwhile, the activities of superoxide dismutase and catalase in the three tissues increased, while the total antioxidant capacity decreased, suggesting that M. unguiculatus have an activated defense mechanism against infection-induced oxidative stress, despite a compromised total antioxidant capacity. Transcriptomic studies reveal that infected M. unguiculatus exhibits upregulation of endocytosis, lysosome activity, cellular apoptosis, and immune-related signaling pathways, indicating that M. unguiculatus responds to pathogen invasion by upregulating efferocytosis. Compared with the gill and adductor muscle, the mantle had a higher level of mytimycin, mytilin and myticin, and the three tissues also increased the expression of mytimycin to cope with the invasion of pathogens. In addition, the analysis of genes related to taste transduction pathways and muscle contraction and relaxation found that after infection with V. alginolyticus, M. unguiculatus may reduce appetite by inhibiting taste transduction in the gill, while improving muscle contraction of the adductor muscle and keeping the shell closed, to resist further invasion of pathogens and reduce the risk of pathogen transmission in the population.

Nutritional condition of wild and hatchery‐reared, green‐lipped mussel (Perna canaliculus) spat used for aquaculture

AbstractThe nutritional condition of seed mussels (spat) at the time of seeding is thought to play a major role in influencing spat losses on mussel farms, with nutritionally compromised spat thought to be more likely to be lost from aquaculture substrata than those in good condition. New Zealand's Greenshell™ mussel (Perna canaliculus) aquaculture industry relies almost exclusively (∼85%) on wild caught spat, with the vast majority, typically &gt; 90%, lost shortly after being seeded onto coastal mussel farms. This study sought to quantify the extent of the variability in the nutritional condition (i.e., calorific, protein, lipid and carbohydrate content) of samples of spat used by the Greenshell™ industry between 2014 and 2021 (i.e., wild caught and hatchery produced) as a first step towards determining whether variability in nutritional condition may be a significant contributor to the marked variation observed in spat performance after it is seeded onto coastal farms. All parameters measured were highly variable among wild spat samples, with all but lipid content varying, including for samples of wild spat harvested at different times within the same month. Furthermore, there were no clear patterns in the nutritional condition of spat across months or years, suggesting environmental factors that limit feeding during the harvest and transport of spat may be contributing to marked differences in the quality of spat being used by the industry to seed farms. These results provide a baseline for assessing the nutritional condition of Greenshell™ spat and point towards a need to develop interventions to improve the quality of wild spat supply for this industry to help and prevent the ongoing massive losses of spat from farms. These measures could include, testing nutritional condition of spat at harvest or prior to seeding, development of nursery culture methods and development of artificial spat feeds that have the potential to replace live phytoplankton during nursery culture.

Hydrodynamic coefficients of mussel dropper lines derived from large-scale experiments and structural dynamics

The expansion of marine aquaculture production is driven by a high market demand for marine proteins and a stagnation of wild catch of fish. Bivalve farming, i.e., the cultivation of oysters, mussels and scallops, is an important part of the ongoing market dynamics and production expansion. As marine spatial planning is considering various use purposes, available space for near-shore aquaculture is already becoming scarce; this has fueled research and development initiatives to enable production installations further offshore. The highly energetic conditions at more exposed offshore marine sites lead to increased loads on aquaculture systems and their components and it is still not sufficiently understood how the load transfer from oceanic environmental conditions onto shellfish-encrusted surfaces attached to elastic ropes may be appropriately quantified. This study data gathered large-scale data sets in a wave tank facility, which are used to validate a novel, numerical model, building on the dynamics of rope structures which allows for the determination of the hydrodynamic loads transferred to the dropper lines. The forces and hydrodynamic parameters are measured and numerically analyzed. Based on the results, drag and inertia coefficients are determined. A drag coefficient of CD=1.1\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$C_\ extrm{D} = 1.1$$\\end{document} and an inertia coefficient of CM=1.7\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$C_\ extrm{M} = 1.7$$\\end{document} are recommended to model shellfish-encrusted dropper lines exposed to oscillatory flows with KC =\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$=$$\\end{document} 40–90. The numerical model for the determination of wave-induced forces on mussel dropper lines is developed and validated using the experimental data. It employs a modified Morison equation, which takes into account the displacement of the mussel dropper line. The influence of varying aquaculture-related parameters is discussed by applying the numerical model. Based on the gathered insights, recommendations can be given from an engineering point of view concerning the optimal placement of mussel aquaculture within the water column.