Mushroom Mycelium Research Articles

OPTIMASI MEDIA DAN TEKNIK STERILISASI UNTUK MENINGKATKAN KUALITAS MISELIUM BIBIT F2 JAMUR TIRAM (Pleurotus ostreotus)

F2 seeds are a derivative of F1 cultivation grown in a medium containing cellulose. Sorghum seeds and corn seeds contain cellulose, hemicellulose, and lignin that can be used as innovative media for F2 seedling development and the growth of oyster mushroom mycelium. The purpose of this research is to determine the growth of F2 oyster mushroom mycelium on media of sorghum seeds and corn seeds that have been sterilized for 40 minutes and 60 minutes. The type of research used is an experiment with a Completely Randomized Design (CRD) in a factorial pattern consisting of 2 factors. Factor 1 is the media type: Sorghum Seeds, and corn Seeds. Factor 2 is the Sterilization Time: 60 minutes and 40 minutes. The parameters measured are the length of the mycelium, the spread of the mycelium, and the thickness of the mycelium in F2 oyster mushroom seedlings. Technical analysis uses quantitative descriptive data. Based on the results obtained regarding the growth of the F2 oyster mushroom mycelium, the highest growth was observed on sorghum seed media, measuring 13.81 cm, with a very thick and dense spread. The cellulose content affects the growth of the mycelium. Meanwhile, the lowest growth of the F2 oyster mushroom mycelium was found on corn seed media, measuring 11.27 cm, with a thin and uneven spread, and a density or thickness that is thin and sparse.

The Development and Consumer Acceptance of Shoe Prototypes with Midsoles Made from Mushroom Mycelium Composite

The Development and Consumer Acceptance of Shoe Prototypes with Midsoles Made from Mushroom Mycelium Composite

Antibacterial and antioxidant activity of the ethyl acetate extract of two strains of Thai Laetiporus sulphureus mushroom

Laetiporus sulphureus, a wood-decaying mushroom, has been reported to be a good source of natural bioactive components. Traditionally used in folk medicine, L. sulphureus has shown its beneficial effects on human health. This study was conducted to evaluate the antibacterial and antioxidant activities of two strains of Thai L. sulphureus mushrooms (MFLUCC 12-0546 and MFLUCC 12-0547) using disc diffusion assay and an ABTS antioxidant assay, respectively. Initially, the mushroom mycelia were prepared and cultured in potato dextrose broth (PDB) and malt extract broth (MEB) at 30°C for 10 days. The ethyl acetate extracts were prepared using the culture supernatants and used for both assays. Although the two mushroom strains were very similar in morphology, they exhibited different antibacterial and antioxidant activities. For antibacterial activity, the MEB extracts had a better effect on antibacterial activity than those obtained from the PDB culture, whereas the PDB extracts showed a better antioxidant activity than those obtained from the MEB cultivation. The results showed that the L. sulphureus extracts obtained from its mycelial state possess antibacterial and antioxidant activity. It should also be noted interestingly that both biological activities tested in the present study are dependent on the mushroom strains and media used for cultivation.

Effect of Differences in the Composition of Growing Media on the Growth of White Oyster Mushroom Mycelium (Pleurotus ostreatus)

The use of the main growth medium in the form of wood sawdust in mushroom domestication is still higher than that of other organic materials, even though its availability is limited, so efforts need to be made to find a growth medium composition that minimizes the percentage of sawdust use. The purpose of this study was to determine the comparison of treatment 1 (80% teak sawdust, 10% bran, 5% tapioca flour, and 5% agricultural lime) with treatment 2 (70% teak sawdust, 15% bran, 10% tapioca flour, and 5% agricultural lime) on the percentage of growth and mycelium growth of white oyster mushrooms. Composting of growing media was carried out for 5 days, then baglog making was carried out, after which the baglog was sterilized for 4 hours. After sterilization, the baglog was cooled and inoculated. After inoculation, the baglog is incubated to grow mycelium. Then, after 15 days, data collection and calculation were carried out. The parameters measured were the percentage of growth and mycelium growth of white oyster mushrooms in baglogs. Results showed that the percentage of growth and mycelial growth of white oyster mushrooms in treatment 1 is better than in treatment 2. Reducing the percentage of the main growing medium of teak sawdust and adding the percentage of additional growing media of bran and tapioca flour has a negative impact on the number of baglogs that grow mycelium (percentage of growth) and the growth of white oyster mushroom mycelium. This means that reducing the main media of teak sawdust and adding additional media is not the right growing media composition for domestication of white oyster mushrooms, so it cannot be used as an alternative growing media composition to reduce the use of wood sawdust.

Revolutionizing Sustainable Fashion: Jute–Mycelium Vegan Leather Reinforced with Polyhydroxyalkanoate Biopolymer Crosslinking from Novel Bacteria

Vegan leather derived from mushroom mycelium is a revolutionary technology that addresses the issues raised by bovine and synthetic leather. Jute–mycelium-based vegan leather was constructed using hessian jute fabric, natural rubber solution, and extracted polyhydroxyalkanoate (PHA) biopolymer from Bacillus subtilis strain FPP-K isolated from fermented herbal black tea liquor waste. The bacterial strain was confirmed using 16S rRNA genomic sequencing. The structural characteristics of sustainable mycelium vegan leather were identified using FTIR, SEM, and TGA methods. To address the functional features of the developed vegan leather, solubility, swelling degree, WVP, WCA, and mechanical strength were also evaluated. Mycelium networking was further validated by micromorphological examination (SEM) of the leather sample’s cross-sectional area. Jute–mycelium leather demonstrated a tensile strength of 8.62 MPa and a % elongation of 8.34, which were significantly greater than the control sample. Vegan leather displayed a strong peak in the O ═ H group of carbohydrates in the examination of chemical bonds. A high-frequency infrared wavelength of 1,462 cm−1 revealed the amide group of protein due to the presence of mycelium, while the absorption peak at 1,703 cm−1 in leather indicated the crosslinking of PHA. Moreover, the TGA study finalized the thermal stability of leather. The enhanced hydrophobicity and reduced swelling degree and solubility also endorsed the water resistance properties of the leather. The results of the investigation substantiated the potential properties of mycelium vegan leather as animal- and environment-free leather.

A Comparative Study of Calcium Sulfate Alternatives in Compost Production for White Button Mushroom (Agaricus bisporus)

This study explores various potential substitutes for gypsum in the production of compost for white button mushrooms (Agaricus bisporus). During compost preparation, calcium sulfate (CaSO4) was replaced with calcium carbonate (CaCO3), ammonium sulfate ((NH4)2SO4), and monocalcium phosphate (Ca(H2PO4)2). Complete replacement of gypsum with calcium carbonate led to a significant pH increase during the second phase of composting, adversely affecting mushroom mycelium growth. Compost parameters were observed to be similar in scenarios where calcium sulfate was supplemented with calcium carbonate in 8:2 and 6:4 ratios, both with and without the presence of ammonium sulfate, and in 3:1 and 1:1 mixtures of calcium sulfate and monocalcium phosphate, when compared to traditional gypsum-based processes. All experimental compost mixtures yielded comparable mushroom crops in cultivation trials. Notably, the 8:2 mixture of calcium sulfate and calcium carbonate demonstrated superior performance in cultivation trials relative to the 6:4 mixture. However, supplementing these mixtures with ammonium sulfate resulted in similar crop yields. Monocalcium phosphate also emerged as a promising partial gypsum substitute, showing comparable crop production in both 3:1 and 1:1 ratios to the technological optimum. The exploration of alternative calcium sources like calcium carbonate and monocalcium phosphate reflects the adaptability of the industry in response to resource availability challenges. The potential use of byproducts like ammonium sulfate from the composting process itself offers a cost-effective and environmentally friendly approach to compost formulation, underscoring its worldwide relevance.

Properties of Chitosan Monofilament from Mushroom Mycelium

Properties of Chitosan Monofilament from Mushroom Mycelium

The Potential of Mycofoam as a Biocomposite Material with Various Substrate and Mushroom Compositions

Mycofoam is a biocomposite composed of mycelial filaments that bind to the host substrate, which can replace polystyrene. This research aims to determine the most appropriate formulation of the growing medium and mushroom to be used in mycofoam products based on some parameters. Composition is one of the main factors for selecting the most appropriate mycofoam to replace polystyrene. On the other hand, the quality of mycofoam can be assessed through its strength and water resistance. The mushroom mycelium used in this study came from Pleurotus ostreatus and Lentinula edodes. Besides, the growing medium formulation comprises five different compositions, each containing different proportions of sawdust and bagasse. This research was composed of preparation of growing medium, spawn inoculation, molding, heating, testing, and data analysis. Based on the results, the combination of 100 percent sawdust and L. edodes was the most appropriate choice compared to other formulations based on strength and appearance. The results of the water absorption test showed that all mycofoam formulations were not resistant to water, bio-based coating can be used to overcome this problem.

Assemblages as Ecologies

In this essay I draw on my art research and installation ‘Earthly Bodies, Subterranean Rhythms’ to posit assemblages as ecologies: platforms where specific agencies and interactions occur at different scales, including microscopic levels. Based on documentation images of my creative process, I delve into my experience exploring sculptural building and creative possibilities in more-than-human collaboration. This research involved observing subterranean bodies and temporalities, rhizomatic growth and mycorrhizal interactions as well as working in interspecies cooperation with oyster mushroom mycelium and wheatgrass roots. As spatial practices, architecture and sculpture have developed specific building methods. I expand on assemblage, the building method I worked with, seeking to articulate three lenses: sculptural, material and philosophical. Technically, an assemblage is a piece made by bringing together disparate elements. In this case it consisted mainly of ceramics, different earthly substrates, mycelium spawn, soil and seeds. Borrowing from Bennet’s ‘Vibrant Matter’ (2010) and Tsing’s ‘The Mushroom at the End of the World’ (2015) I reflect on living sculptures as interspecies assemblages: spaces of collaboration, cooperation and contamination between living and non-living bodies, matter and forces, human and more-than human agencies. From this perspective, assemblages are more than the sum of their parts as they have the capacity to re-make and transform us through encounters. A polyphony of worlding processes makes the collective architecture we live in: our shared world. Working in interspecies assemblages may enable more respectful and enjoyable ways of collective dwelling.

Crude Polysaccharides from Mushrooms Elicit an Anti-Allergic Effect Against Type 1 Allergy In Vitro.

Type 1 allergic disease is a global challenge, hence the search for alternative therapies. Mushrooms have several medicinal and health benefits. However, scant data exist on the anti-allergic properties of polysaccharides from fruiting bodies (FB) and mycelia of mushrooms. We used an in vitro co-culture system comprising Caco-2 cells (intestinal epithelial colorectal carcinoma cell line) and RBL-2H3 cells (cell line from rat basophilic leukemia cells). Reduction in degranulation of mast cells indicated anti-allergy properties. The inhibitory effect of crude polysaccharides from different mushroom FB and mycelia on β-hexosaminidase release from RBL-2H3 cells was measured. Results showed that crude polysaccharides from the FB of Inonotus obliquus exhibited a significant inhibitory effect on β-hexosaminidase release and lowered it by 16%. Polysaccharides from the FB of Lentinus squarrosulus, and Pleurotus ostreatus did not exhibit a significant reduction in β-hexosaminidase. However, crude polysaccharides from their mycelia had a significant inhibitory effect, resulting in up to a 23% reduction in β-hexosaminidase activity. Among fungi showing degranulation properties, crude polysaccharides from their mycelia showed more potent action against degranulation than their corresponding FB. Polysaccharides extracted from FB and or mycelia, of selected mushrooms, possess anti-allergic properties that could be harnessed for use in alternative allergy therapies.

Bio-leather: Sustainable clothing fabrics made from simple media ingredients and slime mold Physarum polycephalum

The textile industry contributes significantly to global warming and pollution, especially the leather industry, which uses livestock and toxic tanning processes that have a great environmental impact. Currently, efforts are being made to mitigate the negative impacts of the textile industry by using alternative non-toxic chemicals or by recycling fabric. More recent efforts explore utilization of non-conventional biomaterials and organisms, such as mushroom mycelia, algae or genetically-engineered microorganisms. In this study, we implemented slime mold Physarum polycephalum perfused through leather-like fabrics made from air-dried simple nutritious media in order to develop environmentally friendly, easy-to-manufacture and sustainable fabrics. Plasmodium was validated for its viability and propagation under non-sterile conditions and in contaminated environments on different media compositions made from agar, peach gum, gelatin, carrageenan or glycerol. We determined optimal media components to be agar, gelatin and glycerol which supported plasmodium growth and yielded sturdy and flexible fabric sheets after air-drying. Ultimately, plasmodium-perfused fabric sheets were sewed into apparel and footwear. This study demonstrates the use of simple media as a clothing fabric perfused with plasmodium, which produces intricate colors and patterns on the fabric. Plasmodium has the ability to enhance fabric properties due to its natural problem-solving abilities, such as biosensing, fabric self-repair, and distant fabric communication.

MEDIA DASAR LIMBAH DAUN KAKAO UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus)

Cocoa leaf wastes as a basic medium are the nutrition that needed for the growth of white oyster mushroom mycelium (Pleurotus ostreatus). The objectives of this study were determine the cocoa leaf wastes as a basic medium for the growth of white oyster mushroom mycelium and the dosage of basic medium to encourage the best fungal growth. This research was designed in a Completely Randomized Design (CRD), consisting of seven treatments and three replications, namely P1 (Basic Media of 100% cocoa leaf waste), P2 (Basic Media 90% + Suplement 10%), P3 (Basic Media 80% + Suplement 20 %), P4 (Basic Media 70% + Suplement 30%), P5 (Basic Media 60% + Suplement 40%), P6 (Basic Media 50% + Suplement 50%), P7 (Sawdust 70%, 20% rice bran + flour corn 10%). The method that used in this study were media preparation, drying, grinding and formulation. The parameters that observed were the basic media, microscopic characteristics of hyphae, mycelium growth curve and macroscopic characteristics of colonies. The results of P1 and P7 showed the best growth for mycelium, observation of mycelium growth were done every 3 days. The isolation stage was carried out on Potato Sucrose Agar (PSA) media, the results obtained that P1 was 0,1 x 1012 CFU/g, P2 was 0,4 x 1012 CFU/g, P3 was 0,3 x 1012 CFU/g, P4 as much as 0,7 x 1012 CFU/g, P5 as much as 0,6 x 1012 CFU/g, P6 as much as 0,4 x 1012 CFU/g, P7 as much as 2,4 x 1012 CFU/g, respectively. Cocoa leaf waste are potentially as a growth medium for mycelium of white oyster mushroom.

Alternative applications of mushroom mycelium for environmental sustainability: opportunities, challenges and future perspective

Alternative applications of mushroom mycelium for environmental sustainability: opportunities, challenges and future perspective

Performance of leaf extract media in culturing mycorrhizal mushroom mycelium

In-vitro culture of mycorrhizal mushroom (MM) species in southern Africa remains largely unexplored, particularly using tree-derived media. In this study, a Julbernardia globiflora [(Benth.) Troupin] leaf infusion was tested for its ability to promote MM mycelial growth. Amanita loosii, Cantharellus miomboensis and Cantharellus heinemannianus isolates were incubated at a pH of 2, 3, 4, 5, 6 or 7 and at 25 °C in six leaf extract agar (LEA) infusion concentrations of 150, 175, 200, 225 or 250 grams of leaves/L distilled water, with potato dextrose agar (PDA) as a standard. We determined mycelium growth rates for all treatment combinations. Mycelium growth rate was found to be optimal at a pH between 4 and 6 in all leaf infusion concentrations tested. Significant (p<0.001) linear regressions of A. loosii and C. miomboensis were found for pH only (R2=0.837 and 0.8582, respectively) and a significant (p<0.001) regression was found for C. heinemannianus (R2=0.293). Amanita loosii and C. heinemannianus had faster (p<0.001) growth in PDA than in LEA, while C. miomboensis had similar growth rates in the two media. Growth characteristics observed were attributed to acid phosphatase mediated physiological processes in mycelium for the different MM species with an optimum pH of 4–6. MM mycelia were white, mycelia for A. loosii and C. miomboensis were loose and for C. heinemannianus were thin filaments. LEA proved to be a potential alternative medium for culturing MM species. Significance: A novel miombo tree extract medium was tested with three miombo mycorrhizal mushrooms. Our findings show the new medium to be a possible alternative to, but not as viable as, potato dextrose agar. The findings of this study widen the scope of use for the forest tree derived media and demonstrate the cultivability of miombo mycorrhizal mushroom species. Our findings improve the possibility of enhancing food security through culturing and possibly cultivating the less explored African mycorrhizal mushrooms.

Multi-property characterization of an experimental material composed of Pleurotus ostreatus mycelium and ash wood chips compared with glass wool and hemp wool

Biomaterials have a lot of potential for the construction industry because of their small environmental footprint and their regenerative capacity. Hemp is currently used as an insulation material in construction. The uniqueness properties of mushroom mycelium allow it to be used as a plant fiber binder in the design of bio-based insulation boards with white ash chips as aggregates. The goal of the present study is to design an organic material that will be 100 % compostable at the end of its useful life while being biologically inactive. The material is composed of the mycelium of Pleurotus ostreatus, an edible fungus, grown on rye grains and white ash chips. The objectives are to develop an experimental protocol for the culture of the material and to characterize it in comparison to glass wool and hemp wool, which are two proven insulators. Once the desired shape is obtained, the mycelium is inactivated by heat. The compressive strength, the conductivity, the thermal effusivity, the volume and the density, the thermal mass capacity and the acoustic properties were measured. The stress resistance was found to be up to 1330 times higher for the experimental material (Medium mycelium) than for glass wool; the thermal conductivity was between 0.0313 and 0.0379 W/(mK) for all materials; the porosity was greater than 75 % for all materials and the α coefficient varied between 0.8 and 1 for sound absorption. Mycelium materials have properties that need to be studied in greater details.

Green and sustainable biomaterials: Edible bioplastic films from mushroom mycelium

Scalable mycelium biomaterials with excellent biodegradable and mechanical properties are attracting widespread attention. However, the inherent brittleness of biomass materials greatly limits their applications in reality, such as packaging and flexibility. In this work, three mycelium films were produced by the capacity of mycelium to spontaneously form three-dimensional biopolymer networks with a toughness of 127.32 kJ/m3 (Ganoderma lucidum), 71.95 kJ/m3 (Auricularia polytricha), and 87.52 kJ/m3 (Pleurotus ostreatus), respectively. After glycerol treatment, the toughness of the films increased by more than 440%, and these films’ surfaces were made smooth and hydrophilic. It has been shown that the hydrogen bonding interactions between glycerol and polysaccharides as well as proteins in mycelium enhance the mechanical and hydration properties of mycelial films. The new strategy of using glycerol to improve the properties of mycelium films will advance on mycelium materials research.

Produksi Biomassa, Analisis Nutrisi dan Senyawa Bioaktif Jamur Grigit (Schizophyllum commune)

Schizophyllum commune, a wild edible mushroom, has gained significant attention due to its potential as a valuable source of biomass, nutrients, and bioactive compounds. This study aims to explore the production of mycelial biomass and analyze its nutrient and bioactive compound content to be applied as a raw material in food production. The Schizophyllum commune was cultivated in potato dextrose broth media with temperature (25, 30, 35, and 40oC) and pH treatments (3, 7, and 8) to determine the optimal conditions for biomass production. Mycelia biomass was harvested and extracted. Nutrient and bioactive compounds were analyzed. Results showed that 30oC and pH 7 give the highest biomass production. Proximate analysis revealed that the mushroom's mycelia biomass contained high protein, low fat, and fiber. The analysis also demonstrated a rich profile of bioactive compounds, including flavonoid and phenolic compounds, respectively1 2,32 ± 0,95 dan 415,72 ± 15,23 g/100 g mycelia dry weight. Schizophyllum commune exhibits promising prospects for mycelial biomass production.

First Record of the Edible Mushroom Lepista sordida in Western Algerian Forest: Nutritional Value and Physicochemical Parameters of Mycelial Culture.

The exploration of the western forests of Algeria led to the remarkable discovery of the first occurrence of Lepista sordida, an edible wild mushroom of significant culinary importance for the local community, traditionally consumed in its natural state. This discovery was made possible through the use of various methods, including macroscopic observations (revealing a violet color) as well as microscopic observations conducted using scanning electron microscopy (SEM), revealing a cylindrical shape with distinct contours. Additionally, molecular analyses were conducted. Genomic DNA was extracted from the mycelium, followed by DNA amplification using specific primers targeting the internal transcribed spacer region (ITS1 and ITS2). After PCR reactions and sequencing of the obtained amplicons, the nucleotide sequences of the mycelium were submitted to the GenBank database of NCBI with the assigned accession number: MZ928450.1. These sequences were subsequently used to construct the phylogenetic tree. Furthermore, an in-depth study of physicochemical parameters was undertaken to determine the optimal conditions for cultivating the mycelium of this edible wild mushroom, including pH, temperature, relative humidity, and light. Different temperatures were examined: 20, 25, 30, 35, 40, and 45 °C. The effect of pH on mycelium growth was studied using a PDA agar medium with buffered values of 4, 5, 5.6, 6, 7, and 8. Similarly, six levels of relative humidity were tested: 14, 50, 74, 80, 95, and 100%. A study on the impact of light on mycelium growth was conducted by exposing Petri dishes inoculated with PDA to a light intensity of 500 lux for 5, 10, 15, 20, and 24 h. The results clearly demonstrated that variations in these different physicochemical parameters significantly influenced mycelium growth. For the Lepista sordida strain, growth was favored at pH levels of 4, 5, 6, and 6, with no growth observed at pH 7 and 8. The optimal temperature range for mycelium growth of Lepista sordida was 20-25 °C, while no growth was observed at 30, 35, 40, and 45 °C. Relative humidity levels of 74, 80, and 95% showed no significant differences. Optimization of mycelium growth and primordia production in Lepista sordida were successfully achieved. Optimal conditions for the primordia phase were identified as 25 °C, with humidity ranging from 90 to 95%. A nutritional analysis of fresh sporophores was conducted using established analytical methods. Notably, the nutritional composition of Lepista sordida sporophores exhibited high significance for the following parameters: moisture content (67.23 ± 1.90%), ash content (9.35 ± 0.66%), fat content (3.25 ± 0.24%), protein content (17.22 ± 0.38%), and carbohydrate content (63.83 ± 1.23%).

Analysis of the activity of oxidative enzymes by multivariate regression in the presence of Mg<sup>2+</sup> oyster mushroom mycelium

Studies have been conducted to optimize the process of deep cultivation of the Oyster mushroom mycelium. The process of obtaining mycelium as a seed for cultivation of fruiting bodies of mushrooms has been improved. The effect of different concentrations of magnesium carbonate (Magnesium carbonates) on the growth characteristics of mycelium of the fungus Pleurotus ostreatus during deep cultivation has been studied. The dependence of enzyme activity on the concentration of metal in the nutrient medium of germinating mycelium of oyster mushrooms has been revealed. The method for determining the activity of catalase by spectrophotometric method has been adapted for the studied objects. For the first time the data on the activity of mycelium catalase and superoxide dismutase enzymes in the presence of magnesium carbonate additive have been obtained. It has been found that the application of magnesium carbonate in low concentrations has a positive effect on the growth of mycelial biomass of the fungus Pleurotus ostreatus, since with increasing concentration (Mg2+) a decrease in biomass growth rate and catalase activity has been observed, presumably due to the participation of magnesium in creating a certain ionic concentration at which catalase inactivation begins. The possibility of applying the method of multivariate regression in the form of the principal components analysis (PCA) has been studied. The redox state of Pleurotus ostreatus culture at the level of enzyme components of the antioxidant defense system during submerged cultivation of basidomycetes has been analyzed, which showed how the obtained variables with different measurement units are interconnected. The account graphs also clearly indicate the dependence of mycelial growth on the concentration of the additive used. The introduction of the cultivation conditions proposed in this work in the practice of mushroom production potentially contributes to a more successful resistance of macromycetes to biotic and abiotic stress. The results of the research are relevant to the development of the fundamentals of the science of fungi.

Whitening Activity of Acteoside from Stachys sieboldii Fermented with Hericium erinaceus Mycelia on Melanocytes

Skin whitening has recently renewed attention on Chinese herbal medicines with whitening activity for esthetic applications. Stachys sieboldii has been used as herbal medicine since ancient times and has the potential for development as a cosmetic material because of its astringent effect. In this study, with an aim to develop new functional materials with whitening effects, S. sieboldii water extracts were fermented with different mushroom mycelia. Fermented with Hericium erinaceus mycelia showed the strongest tyrosinase inhibition effect and the lowest melanin content. Thus, H. erinaceus mycelia, the most potent inhibitor of melanogenesis, was used for large-scale fermentation and fractionated. The ethyl acetate fraction, which had the strongest whitening activity, was separated and purified using HPLC. Finally, the single compound was isolated and identified as acteoside, which has promising whitening activity. Acteoside inhibited melanin synthesis and intracellular tyrosinase activity in a dose-dependent manner. The effects of acteoside on the expression of TYR, TRP-1, TRP-2, and MITF were analyzed using Western blot analysis, which showed that acteoside reduced the protein in a dose-dependent manner. Our findings reveal the potential applicability of S. sieboldii extract fermented with H. erinaceus mycelia and its useful component, which is an acteosid, for skin lightening and the treatment of pigmentation.