Mushroom Fruiting Research Articles

Preparation of Mycelium Pulp from Mushroom Fruiting Bodies

Preparation of Mycelium Pulp from Mushroom Fruiting Bodies

Impact of Substrate Volume on Oyster Mushroom Fruiting Bodies Production

Impact of Substrate Volume on Oyster Mushroom Fruiting Bodies Production

THE EFFECTS OF TEMPERATURE AND MOISTURE STRESS CONTENT ON THE EXTENSIVE CULTIVATION OF THE OYSTER MUSHROOM

Oyster mushroom (Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm.) is involved in the destruction of dead wood which is the main place of settlement of several living organisms. After humification, dead wood also becomes an important component of forest soils. The purpose of the research is to study temperature and moisture conditions of extensive cultivation of oyster mushrooms on various wood substrates. To accomplish this goal, the following tasks were set: to determine the amount of effective stress temperatures and moisture content of substrates and their influence on the appearance of fruiting bodies of the oyster mushroom; to study the features of the extensive cultivation of oyster mushrooms on the tree stumps of cutover areas, in the hollows of broadleaved trees, using brushwood, on inoculated log sections in trenches, on log sections of dead dry trees. The oyster mushroom fruiting bodies collected in the suburban forest formed the basis of the experiment on the inoculation of different types of substrates. The inoculation of the log sections was carried with a relative air humidity of 85%, the process of overgrowing lasted from 2 to 3 months. The edaphic-climatic factors of different types of space - closed and open - were taken into account. The amount of effective temperatures necessary for the development of fruiting bodies has been determined. It was found that the intensive growth of the mushroom fruiting bodies begins after a sharp cooling (a drop in night temperatures to 4-8 ° C), which causes a stressful state of the mushroom. The amount of the effective stress temperatures is 4.6 ° C. Long-term phenological observations have revealed that the amount of the effective temperatures in the spring is 76.4 ° C, and in the autumn - 59.4 ° C. The duration of fruiting of oyster mushroom on log sections of hardwoods is 14 days in spring and 17 days in autumn longer than on softwoods blocks. It was found that the biomass of the fruiting bodies is directly dependent on the thickness of the log section, and, therefore, it is recommended to use log sections ranging from 35 cm to 50 cm in diameter and up to 30 cm in length.

Polysaccharides from Mushroom Fruiting Bodies Improve Insulin Resistance by Inhibiting Lipid Deposition Via Autophagy Enhancement

Insulin resistance is the main cause of type 2 diabetes. Polysaccharide is one of the main active components in mushrooms and has been reported that certain mushroom polysaccharides have beneficial effects against type 2 diabetes. However, the structural features and mechanisms of polysaccharides with hypoglycemic activity have not been elucidated. In this paper, six alkali-soluble total polysaccharides, six neutral polysaccharides and six acidic polysaccharides were extracted and purified from mushrooms fruiting bodies of different species. The effects of these polysaccharides on improving insulin resistance were compared using high fatty acids and glucose-induced hepatocytes. Among them, the neutral polysaccharide AAMP-N of Armillaria mellea fruiting body showed the most significant activity, suggesting that galactoglucan is the main active domain. AAMP-N inhibited intracellular fat accumulation and enhanced insulin sensitivity by activating autophagy. Autophagy is an essential lysosomal degradation pathway implicated in the development of type 2 diabetes. Consistent with the in vitro results, AAMP-N enhanced hepatic autophagy, lowered blood glucose, and modulated lipid metabolism in db/db mice. In addition, AAMP-N also protected damaged pancreatic islets in mice. Our results demonstrated for the first time a role of natural polysaccharides from mushrooms in the improvement of insulin sensitivity and metabolism, and provided basis for the development of mushroom polysaccharides as hypoglycemic healthy food. Funding Statement: This work was supported by National Natural Science Foundation of China (No. 31872674), Jilin Province Pharmaceutical Industry Development Fund (No. 20180311068YY), Jilin Province Development and Reform Commission (2018C047-2), and Fundamental Research Funds for the Central Universities (No. 2412017FZ018). Declaration of Interests: The authors have declared no conflicts of interest. Ethics Approval Statement: All experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals of Northeast Normal University.

Agar plate assays using dye-linked substrates differentiate members of Tricholoma sect. Caligata, ectomycorrhizal symbionts represented by Tricholoma matsutake

Tricholoma matsutake is an ectomycorrhizal fungus that produces the prized mushrooms “matsutake” in association with the Pinaceae. Other species of Tricholoma sect. Caligata are also ectomycorrhizal symbionts that produce a variety of “quasi-matsutake” mushrooms. Here we developed agar plate assays using the dye-linked substrates azurine-crosslinked (AZCL) hydroxyethyl cellulose and AZCL-amylose to differentiate T. matsutake strains and related species based on their polysaccharide-degrading activities. This method may be useful for screening strains that adapt well to spawn cultivation for mushroom fruiting.

Record breaking mushroom yields in Spain

Abstract Despite the assumption that mushroom fruiting is dependent on climate conditions, recent changes in temperature and precipitation regimes in Mediterranean-type ecosystems have opened new questions about how climate changes impact epigeous sporocarps yields. Here, we described the epigeous fungal sporocarp yield anomalies together with temperature and precipitation fluctuations that have triggered atypical fungal production peaks during the last 20 years in different forest ecosystems of Mediterranean Spain. We used the largest standardized, spatio-temporal epigeous sporocarp collection dataset available in Mediterranean-type forest ecosystems and climates. Two clear positive yield anomalies were found in 2006 and 2014, representing more than 270% and 210% increase, respectively, in comparison with time-series mean annual fungal yields. Late-summer-early-autumn precipitation was the most influential variable triggering these extreme mushroom production peaks in comparison with temperature. This suggests that fungal fruiting is sensitive to late-summer-early-autumn precipitation pulses, producing discrete yield pulses when conditions are optimal, which are interspersed among periods of limited water resource availability and lower yields.

Content of Purine in Mushroom Fruiting Bodies and Mycelia

Content of Purine in Mushroom Fruiting Bodies and Mycelia

Introducing Mushroom Fruiting Patterns from the Swiss National Poisons Information Centre.

Changes in the ecology of macrofungi are poorly understood, not only because much of their life cycle is hidden belowground, but also because experiments often miss real-world complexity and most fruitbody inventories are limited in space and time. The National Poisons Information Centre ‘Tox Info Suisse’ provides countrywide 24hours/7days medical advice in case of poisonings since 1966. Here, we introduce a total of 12,126 mushroom-related phone calls that were received by Tox Info Suisse between 1966 and 2014. This indirect source of mycological information is dominated by the families of Boletaceae (11%), Agaricaceae (10%) and Amanitaceae (8%), which account for ~30% of all cases. Mushroom fruiting patterns revealed by the Poisons Centre inventory statistically resemble changes in fungal phenology, productivity and diversity as reflected by the Swiss National Data Centre ‘SwissFungi’. Although the newly developed Tox Info Suisse dataset provides an innovative basis for timely environmental research, caution is advised when interpreting some of the observed long-term changes and autumnal extremes. Uncertainty of the new record relates to possible data incompleteness, imprecise species description and/or identification, as well as the inclusion of cultivated and non-indigenous mushrooms. Nevertheless, we hope that the Tox Info Suisse inventory will stimulate and enable a variety of ecological-oriented follow-up studies.

ChemInform Abstract: Chemistry, Nutrition, and Health‐Promoting Properties of Hericium Erinaceus (Lion′s Mane) Mushroom Fruiting Bodies and Mycelia and Their Bioactive Compounds

AbstractReview: 139 refs.

Chemistry, Nutrition, and Health-Promoting Properties of Hericium erinaceus (Lion's Mane) Mushroom Fruiting Bodies and Mycelia and Their Bioactive Compounds.

The culinary and medicinal mushroom Hericium erinaceus is widely consumed in Asian countries, but apparently not in the United States, for its nutritional and health benefits. To stimulate broader interest in the reported beneficial properties, this overview surveys and consolidates the widely scattered literature on the chemistry (isolation and structural characterization) of polysaccharides and secondary metabolites such as erinacines, hericerins, hericenones, resorcinols, steroids, mono- and diterpenes, and volatile aroma compounds, nutritional composition, food and industrial uses, and exceptional nutritional and health-promoting aspects of H. erinaceus. The reported health-promoting properties of the mushroom fruit bodies, mycelia, and bioactive pure compounds include antibiotic, anticarcinogenic, antidiabetic, antifatigue, antihypertensive, antihyperlipodemic, antisenescence, cardioprotective, hepatoprotective, nephroprotective, and neuroprotective properties and improvement of anxiety, cognitive function, and depression. The described anti-inflammatory, antioxidative, and immunostimulating properties in cells, animals, and humans seem to be responsible for the multiple health-promoting properties. A wide range of research advances and techniques are described and evaluated. The collated information and suggestion for further research might facilitate and guide further studies to optimize the use of the whole mushrooms and about 70 characterized actual and potential bioactive secondary metabolites to help prevent or treat human chronic, cognitive, and neurological diseases.

Habitat configuration affects spatial pattern of β diversity of insect communities breeding in oyster mushrooms

Theory predicts that spatial structure can mediate interactions that affect species diversity in a patchy environment. A rarely considered effect of spatial structure on biodiversity is the interplay of spatial habitat arrangement with species interactions at multiple spatial scales. We investigated how spatial habitat arrangement and predation mediate the assembly of the larval communities of fungivorous insects breeding in the oyster mushroom, Pleurotus ostreatus (Jacq. ex Fr.) P.Kumm in a North American woodland. In a two‐way factorial design, we varied the spatial arrangement of mushroom clumps (‘clustered', ‘patchy', and ‘uniform'; 3 levels) crossed with predator exclusion (access allowed or not; 2 levels) to study their joint effects on patterns of α, β and γ diversity of the fungivorous insect communities. Partitioning diversity into these three components suggested that neither spatial nor predation treatments significantly affected α, β and γ diversity. We found that an intermediate inter‐clump distance (i.e., the ‘patchy' treatment) increased spatial autocorrelation in insect community composition within experimental blocks, particularly in the mushrooms to which predators had access. The spatial structuring in β diversity indicates that the arrangement of mushroom clumps can structure β diversity of fungivorous larval communities through direct effects on the species themselves (e.g., increased aggregation and habitat choice of ovipositing females of fungivorous insects), as well as effects mediated through the presence and behavior of predators (e.g., spatially structured selective foraging by predators acting as a filter on which species were in the clumps). The naturally patchy nature of mushroom fruiting may transform the spatial pattern of β diversity by altering the behavior of ovipositing females, or by weakening the negative effect of larval competition.

Unraveling environmental drivers of a recent increase in Swiss fungi fruiting

Disentangling biotic and abiotic drivers of wild mushroom fruiting is fraught with difficulties because mycelial growth is hidden belowground, symbiotic and saprotrophic supply strategies may interact, and myco-ecological observations are often either discontinuous or too short. Here, we compiled and analyzed 115417 weekly fungal fruit body counts from permanent Swiss inventories between 1975 and 2006. Mushroom fruiting exhibited an average autumnal delay of 12days after 1991 compared with before, the annual number of fruit bodies increased from 1801 to 5414 and the mean species richness doubled from 10 to 20. Intra- and interannual coherency of symbiotic and saprotrophic mushroom fruiting, together with little agreement between mycorrhizal yield and tree growth suggests direct climate controls on fruit body formation of both nutritional modes. Our results contradict a previously reported declining of mushroom harvests and propose rethinking the conceptual role of symbiotic pathways in fungi-host interaction. Moreover, this conceptual advancement may foster new cross-disciplinary research avenues, and stimulate questions about possible amplifications of the global carbon cycle, as enhanced fungal production in moist mid-latitude forests rises carbon cycling and thus increases greenhouse gas exchanges between terrestrial ecosystems and the atmosphere.

Isolation and characterization of differentially expressed genes in the mycelium and fruit body of Pleurotus ostreatus

The fruiting body of one of the most widely cultivated mushrooms, the oyster mushroom (Pleurotus ostreatus) is highly interesting, both commercially and scientifically. In the present study, we performed comparative proteomic profiling of P. ostreatus at two unique developmental stages; mycelium and fruit body, using two-dimensional gel electrophoresis (2-DE). Seven hundred fourteen (714) spots were detected and 29 spots (showing a high level of difference in their expressions) were identified by tandem mass spectrometry and basic local alignment search tool (BLAST) searching of an expressed sequence tag (EST) database of P. ostreatus. Among them, six proteins (putative fatty acid oxygenase, heat shock sks2, PriA homologue, Ap-1 like transcription factor YAP7, mung bean seed albumin, and C2H2 Zinc finger domain protein) and one protein (peroxisomal biogenesis factor 6) showed increased expression levels at the fruiting process and the mycelial stage, respectively. Through reverse transcriptase-polymerase chain reaction analysis, priA homologue and AP-1 like transcription factor yap7 showed gradually increased expression from mycelia to fruit body, whereas putative fatty acid oxygenase and heat shock protein sks2 were expressed only in the fruit body. These results provide useful information for future studies of mushroom development of P. ostreatus. Keywords : Developmental stage, mushroom fruiting, Pleurotus ostreatus , protein, two-dimensional gel electrophoresis African Journal of Biotechnology Vol. 12(24), pp. 3790-3796

천마버섯(Armillaria gallica )의 생태학적 서식지 조사

Armillaria gallica was ecologically surveyed to investigate its relationship with Gastrodia elata in cultivation areas of Korea in 2012. In the observation made around October 17 in the area of Namyangju (Gyeonggi), Sangju (Gyeongbuk) and Gimcheon (Gyeongbuk) in Korea, the fruit bodies of A. gallica were consistently found near the cultivation areas of Gastrodia elata across these cultivation areas. Since the temperature and rainfall have been considered as important factors of fruiting of A. gallica, we checked the temperature and rainfall around two weeks ago of mushroom fruiting. The average temperature of all cultivation areas was (the lowest temp.: , the highest temp.: ) and the annual rainfall was 0.5~1.0mm on October 10 which can be compared to 2.5~4.5mm on October 17. Fruiting bodies were generally developed at weedy field in which G. elata were cultivated five years ago. The field was inclined 15 degrees, east-facing and consists of loam. The fruit bodies were formed on both sides of a small ditch between the Gastrodia elata cultivation fields and neighboring fields, and the areas were common weeds and moisture at all times. The fruiting bodies were growing above the soil rather than oak wood. Gregarious, but also occurs to 1-5 fruiting bodies occur sporadically. Results observed in detail the growing areas, some fruit bodies were developed along the black and thick rhizomorph formation.

A Thirty-Year Survey Reveals That Ecosystem Function of Fungi Predicts Phenology of Mushroom Fruiting

Mushroom fruiting, the reproduction of fungi, has broad implications for forest health, terrestrial biomass turnover, and global carbon cycle. However, little is known about the difference in phenology and environmental drivers of mushroom fruiting between functional guilds, e.g., ectomycorrhizal (ECM) mutualists and saprotrophs (SAP). There is a remarkable difference between ECM and SAP fungi in their available carbon sources and lifecycles, and thus these fungal groups are likely to differ in fruiting phenology. We analyzed intra- and inter-annual phenological patterns of mushroom fruiting throughout the year using a long-term census dataset of mushroom-forming fungi in a Japanese oak forest in which a total of 11,923 mushroom counts (668 species) were recorded during monthly intervals from 1982 to 2011. ECM fungi showed a unimodal seasonal fruiting peak from mid-summer to early autumn; litter-decomposing fungi showed moderate fruiting peaks from early summer or early autumn, and the phenology of wood-decomposing fungi varied considerably among the genera. Each functional group was controlled by a different set of external factors; temperature and rainfall increased ECM fungal fruiting, but key factors substantially differed among the genera of litter- and wood-decomposing fungi in taxon-specific ways. Our results suggest that fungal fruiting phenology may be affected by the seasonality of carbohydrate availability. The highly scheduled reproduction of ECM fungi may reflect temperature-dependent increases and drought-induced decreases of photosynthetic activity in host plants rather than improved growth conditions for fungi during the summer. We argue that the way a fungus obtains carbohydrates may explain a substantial fraction of the fruiting phenology, which may make a differential contribution to the community structure of fungus-associated organisms and terrestrial biomass turnover based on fungal functional groups.

Climate change effects fruiting of the prize matsutake mushroom in China

Climate change affects various facets of life but there is little data on its effects on wild mushroom fruiting. Yunnan Province in China is a rich source of wild mushrooms and has experienced a temperature rise over recent decades. This has resulted in warmer temperatures but the impacts of these changes on mushroom production lack documentation. We collected data on the fruiting of the highly prized matsutake mushroom (Tricholoma matsutake) in West Yunnan, China over an 11 year period from 2000 to 2010. Fruiting phenology and productivity were compared against the driving meteorological variables using Projection to Latent Structure regression. The mushrooms appeared later in the season during the observation period, which is most likely explained by rising temperatures and reduced rain during May and June. High temperature and abundant rain in August resulted in good productivity. The climate response of matsutake production results from a sequence of processes that are possibly linked with regulatory signals and resource availability. To advance the knowledge of this complex system, a holistic research approach integrating biology, ecology, genetics, physiology, and phytochemistry is needed. Our results contribute to a general model of fungal ecology, which can be used to predict the responses of fungi to global climate change.

Antioxidant Activity of Crude Polysaccharides from Edible Fresh and Dry Mushroom Fruiting Bodies of Lentinus sp. Strain RJ-2

Antioxidant Activity of Crude Polysaccharides from Edible Fresh and Dry Mushroom Fruiting Bodies of Lentinus sp. Strain RJ-2

Lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids

Solid waste from anaerobic digestion of litter from the commercial production of broiler chickens has limited use as fertilizer. Its disposal is a major problem for digester operators who are seeking alternative use for anaerobic digester solids, also referred to as solid waste (SW). The use of SW as substrates for the cultivation of Pleurotus ostreatus strain MBFBL400 was investigated. Lignocellulolytic enzymes activity, substrate utilization, and mushroom yield were evaluated in ten different substrate combinations (SCs) containing varying amounts of solid waste, wheat straw, and millet. Nutritional content of mushrooms produced on the different substrates was also determined. Substrates containing 70-80% wheat straw, 10-20% SW, and 10-20% millet were found to produce the highest mushroom yield (874.8-958.3 g/kg). Loss of organic matter in all SCs tested varied from 45.8% to 56.2%, which had positive correlation with the biological efficiency. Laccase, peroxidase, and carboxymethylcellulase (CMCase) activities were higher before fruiting, whereas xylanase showed higher activities after mushroom fruiting. SW increased the nutritional content in mushrooms harvested, and the combination of wheat straw and SW with millet significantly improved mushroom yield. Our findings demonstrated the possibility of utilizing anaerobic digester solids in mushroom cultivation. The application of SW as such could improve the financial gains in the overall economy of anaerobic digester plants.

Ergosterol and Water Changes in Tricholoma matsutake Soil Colony during the Mushroom Fruiting Season

The purpose of this study is to understand spatio-temporal changes of active fungal biomass and water in Tricholoma matsutake soil colonies during the mushroom fruiting season. The active fungal biomass was estimated by analyzing ergosterol content at four different points within four replicated locations in a single circular T. matsutake colony at Ssanggok valleyin the Sogri Mt. National Park in Korea during 2003 to 2005. The four points were the ahead of the colony, the front edge of the colony and 20 cm and 40 cm back from the front edge of the colony. Ergosterol content was 0.0 to 0.7 μg per gram dried soil at the ahead, 2.5 to 4.8 μg at the front edge, 0.5 to 1.8 μg at the 20 cm back and 0.3 to 0.8 pg at the 40 cm back. The ergosterol content was very high at the front edge where the 7. matsutake hyphae were most active. However, ergosterol content did not significantly change during the fruiting season, September to October. Soil water contents were lower at the front edge and 20 cm back from the front edge of the colony than at the ahead and 40 cm back during the fruiting season. Soil water content ranged from 12 to 19% at the ahead, 10 to 11% at the edge, 9 to 11% at the 20 cm back and 11 to 15% at the 40 cm back. Our results suggest that the active front edge of the T. matsutake soil colony could be managed in terms of water relation and T. matsutake eetomyeorrhizal root development.

Mushroom fruiting and climate change

Many species of fungi produce ephemeral autumnal fruiting bodies to spread and multiply. Despite their attraction for mushroom pickers and their economic importance, little is known about the phenology of fruiting bodies. Using approximately 34,500 dated herbarium records we analyzed changes in the autumnal fruiting date of mushrooms in Norway over the period 1940-2006. We show that the time of fruiting has changed considerably over this time period, with an average delay in fruiting since 1980 of 12.9 days. The changes differ strongly between species and groups of species. Early-fruiting species have experienced a stronger delay than late fruiters, resulting in a more compressed fruiting season. There is also a geographic trend of earlier fruiting in the northern and more continental parts of Norway than in more southern and oceanic parts. Incorporating monthly precipitation and temperature variables into the analyses provides indications that increasing temperatures during autumn and winter months bring about significant delay of fruiting both in the same year and in the subsequent year. The recent changes in autumnal mushroom phenology coincide with the extension of the growing season caused by global climate change and are likely to continue under the current climate change scenario.