Diseases Of Musculoskeletal System Research Articles

DIAGNOSTICS OF POSTURAL DISORDERS IN CHILDREN USING COMPUTER PHOTOMETRY

Actuality of the research. Literary and statistical data show that the level of morbidity among children in Ukraine remains high. Important to note that musculoskeletal system and connective tissue diseases take the fifth place among the morbidity structure, while the postural disorders and scoliosis remain the most urgent problem in school­age children. The aim of study is to check the computer photometry method efficiency for postural disorders diagnostics in school­age children with different levels of motor activity. Research methodology. In order to improve the dynamic control over the posture state, to increase the study objectification, to reduce the examination time and simplify the statistical material processing, a software complex for diagnosing posture disorders using the computer photometry method was used. Results. It was found that 25% of examined school­age children had postural disorders. Most often, postural disorders were observed within the age of 10­13 years, while in girls, postural disorders were registered 10% more often than in boys. Systematic exercise does not automatically guarantee the correct posture formation. In children­athletes, more pronounced indicators of angular anthropometric parameters asymmetry were registered, as a result of asymmetric back muscles load distribution. Children who did not exercise were more prone to kyphotic posture developing. Conclusions. As instrumental diagnostics, the computer photometry method has high level of informativeness and reliability, helps to maximize the data objectification and is effective in postural disorders detecting during as screening and as mass examinations.

Current challenges for therapy of comorbid patients: a new look at celecoxib. A review

The use of non-steroidal anti-inflammatory drugs (NSAIDs) for a wide range of diseases is increasing, in part due to an increasing elderly population. Elderly patients are more vulnerable to adverse drug reactions, including side effects and adverse effects of drug-drug interactions, often occurring in this category of patients due to multimorbidity and polypharmacy. One of the most popular NSAIDs in the world is celecoxib. It is a selective cyclooxygenase (COX)-2 inhibitor with 375 times more COX-2 inhibitory activity than COX-1. As a result, celecoxib has a better gastrointestinal tract safety profile than non-selective NSAIDs. Gastrointestinal tolerance is an essential factor that physicians should consider when selecting NSAIDs for elderly patients. Celecoxib can be used in a wide range of diseases of the musculoskeletal system and rheumatological diseases, for the treatment of acute pain in women with primary dysmenorrhea, etc. It is also increasingly used as part of a multimodal perioperative analgesia regimen. There is strong evidence that COX-2 is actively involved in the pathogenesis of ischemic brain damage, as well as in the development and progression of neurodegenerative diseases, such as Alzheimer's disease. NSAIDs are first-line therapy in the treatment of acute migraine attacks. Celecoxib is well tolerated in patients with risk factors for NSAID-associated nephropathy. It does not decrease the glomerular filtration rate in elderly patients and patients with chronic renal failure. Many meta-analyses and epidemiological studies have not confirmed the increased risk of cardiovascular events reported in previous clinical studies and have not shown an increased risk of cardiovascular events with celecoxib, irrespective of dose. COX-2 activation is one of the key factors contributing to obesity-related inflammation. Specific inhibition of COX-2 by celecoxib increases insulin sensitivity in overweight or obese patients. Combination therapies may be a promising new area of treatment for obesity and diabetes.

Features of the biological development of schoolchildren in the Moscow region with different levels of health

BACKGROUND: The study of the health status of children and the dynamics of the physical and biological development of schoolchildren remains an urgent scientific issue both in our country and worldwide. The lifestyles of modern children and adolescents change based on regional standards for physical and biological development. AIM: To analyze the dynamics of puberty in modern schoolchildren depending on their health status and update biological development standards of schoolchildren in the Moscow region. MATERIALS AND METHODS: In the 2020s, an observational single-center cross-sectional study of the health status and physical and biological development of schoolchildren aged 12–16 years was performed in Moscow. Physical and biological development was assessed in each age and sex group according to standard methods using certified anthropometric instruments to measure height and weight. RESULTS: The study involved 508 schoolchildren with different levels of health, who were divided into groups 1 (n=107, 21.1%), 2 (n=208, 58.7%), and 3 (n=103, 20.2%). Diseases of the eye and its adnexal apparatus (24%) were prevalent functional abnormalities, and musculoskeletal system and connective tissue diseases were found in 20% of the sample. The level of biological development in groups 1 and 2 in comparison with group 3 did not differ significantly (р 0.05); however, later development of secondary sexual characteristics tended to occur in schoolchildren with chronic diseases. Significant (р=0.038) differences in the age at menarche in girls were found depending on the level of physical development. Thus, when developing standards for biological development, data of schoolchildren in groups 1 and 2 must be considered. CONCLUSION: The results of this study clearly demonstrated the prevalence of functional abnormalities and chronic diseases of various nosologies among schoolchildren in the Moscow region. The results of the study provided an update on the standards for the biological development of schoolchildren in Moscow.

The impact of ageing mechanisms on musculoskeletal system diseases in the elderly.

Ageing is an inevitable process that affects various tissues and organs of the human body, leading to a series of physiological and pathological changes. Mechanisms such as telomere depletion, stem cell depletion, macrophage dysfunction, and cellular senescence gradually manifest in the body, significantly increasing the incidence of diseases in elderly individuals. These mechanisms interact with each other, profoundly impacting the quality of life of older adults. As the ageing population continues to grow, the burden on the public health system is expected to intensify. Globally, the prevalence of musculoskeletal system diseases in elderly individuals is increasing, resulting in reduced limb mobility and prolonged suffering. This review aims to elucidate the mechanisms of ageing and their interplay while exploring their impact on diseases such as osteoarthritis, osteoporosis, and sarcopenia. By delving into the mechanisms of ageing, further research can be conducted to prevent and mitigate its effects, with the ultimate goal of alleviating the suffering of elderly patients in the future.

The study of marketing opportunities for the use of the raw material of feverfew (Tanacetum parthenium L.) in the pharmacotherapy of musculoskeletal system diseases

Aim. To form a modern idea of the market structure and trends in the consumption of drugs for the pharmacotherapy of rheumatoid arthritis, to determine the possible potential for use of plant raw material of feverfew (Tanacetum parthenium). Materials and methods. The study was conducted using the structural analysis, logical and graphical methods, and marketing analysis methods. Results. It was found that in the pre-war period the market for drugs affecting the musculoskeletal system developed quite vigorously – its growth rate in the period of 2020-2021 ranged from 16 to 29 % in natural units and from 20 to 30 % in monetary terms. According to the structure of producing countries, the market was significantly import-dependent. The market for synthetic drugs was quite saturated, represented by various dosage forms – tablets, powder for suspension, injection solutions (ampoules), granules, capsules, sachets, alcohol solutions, oil solutions, suppositories, oral suspensions, transdermal patches, gels, ointments. The research was conducted taking into account the study of marketing opportunities for the use of the raw material of Tanacetum parthenium in the pharmacotherapy of diseases of the musculoskeletal system since this raw material had certain advantages compared to synthetic analogs. It should be noted that the market of herbal medicines for the treatment of the musculoskeletal system diseases or the prevention of their manifestations is very limited and is represented by a minimum number of product items and one representative of the medicinal flora – Symphytum officinale. In addition, 90 % of the market for this group of drugs belongs to foreign manufacturers. Although research of the market for biological supplements indicates a certain demand for products based on the medicinal plant raw material. Conclusions. Based on the trends identified, we can state the positive development of the market for drugs that affect the musculoskeletal system, the prospects for the development of drugs based on the medicinal plant raw material, including Tanacetum parthenium.

Particularities of the bone tissue regenerative properties of the proximal part of the tibia in patients with rheumatoid arthritis with multi-plane knee deformities

Background. Rheumatoid arthritis (RA) has no equal among the musculoskeletal system diseases in terms of the severity of joint damage. Knee joint damage was noted in 65–70 % of RA patients and it is the most common cause of disability. The purpose was to study the indices of osteogenic activity of stromal (mesenchymal) stem cells (MSCs) of the articular bone ends of the knee joint in patients with secondary knee osteoarthritis (KOA) due to RA in the presence of multi-plane deformations. Materials and methods. The studied material was samples of cancellous bone of the anterior and posterior parts of the plateau of the proximal part of the tibia and spongiosa from a depth of 1 cm and 3 cm from the articular surface, namely from the proximal epimetaphysis of the tibia, removed during knee arthroplasty of patients with secon­dary KOA due to RA and in patients with primary KOA in the presence of multi-plane deformations. Using the method of cloning colony-forming units of fibroblasts (CSF) of bone tissues, 38 patients were examined, including 9 patients with RA and 29 patients with primary KOA with damage to the knee joint in the presence of multi-plane deformations. Results. The total number of nucleated cells in 1 cm3 of spongiosa of the front part of the tibial plateau was 2.3 times lower compared to the parameter of this index in the posterior part (p < 0.05). The characteristics of spongy tissue in terms of the number of nucleated cells and the content of CSF in 1 cm3 from a depth of 3 cm from the articular surface of the proximal epimetaphysis of the tibia of patients with RA were almost 3 times higher compared to the indices of patients with primary KOA (p < 0.05). Conclusions. Changes in the renovation processes of bone tissue in patients with secondary KOA due to RA and in patients with primary KOA in the presence of multi-plane deformations are unidirectional, but their expressiveness is greater in patients with RA. At a depth of 3 cm from the articular surface of the proximal epimetaphysis of the tibia, the conditions for secondary fixation of the leg of the knee joint replacement are much better, all other conditions being equal, so the "survival" of the endoprosthesis with an elongated leg will be greater.

Incidence of the musculoskeletal system diseases in children and the organization of specialized care in Saint Petersburg

BACKGROUND: The high prevalence of musculoskeletal system and connective tissue diseases, effects on the quality of life of children, and disability level are among the main medical and social problems.
 AIM: To assess the morbidity rates and provision of medical care to St. Petersburg children with musculoskeletal diseases between 2017 and 2021.
 MATERIALS AND METHODS: Data on the incidence of musculoskeletal diseases in children of St. Petersburg were analyzed based on the Federal statistical observation forms submitted by the St. Petersburg State Budgetary Institution Medical Center for Information and Analysis, collections of N.N. Priorov National Medical Research Center for Traumatology and Orthopedics of the Ministry of Health of the Russian Federation “Trauma, orthopedic morbidity, the state of trauma and orthopedic care for the population,” edited by Acad. S.P. Mironov, RAS, and data from the Federal State Statistics Service.
 RESULTS: In Saint Petersburg, the morbidity rate of musculoskeletal and connective tissue diseases from 2017 to 2021 was 13%, excluding 2020, which was most likely due to the introduction of measures aimed at counteracting the spread of COVID-19 and the suspension of planned outpatient specialized care. The number of orthopedic beds for children decreased by 58, whereas the availability of beds for children remained at the same level and amounted to 4.3 beds per 10,000 children, which exceeded the indicator for the Russian Federation (1.0). The number of children with these disabilities increased 1.4-fold. Deaths of children due to musculoskeletal and connective tissue diseases were recorded in 2019 and 2020.
 CONCLUSIONS: The results of the 5-year observation period of musculoskeletal and connective tissue diseases in children in St. Petersburg revealed increasing incidence because of the increase in the number of children newly diagnosed with these diseases.

Impacts of β-1, 3-N-acetylglucosaminyltransferases (B3GNTs) in human diseases.

Glycosylation modification of proteins is a common post-translational modification that exists in various organisms and has rich biological functions. It is usually catalyzed by multiple glycosyltransferases located in the Golgi apparatus. β-1,3-N-acetylglucosaminyltransferases (B3GNTs) are members of the glycosyltransferases and have been found to be involved in the occurrence and development of a variety of diseases including autoimmunity diseases, cancers, neurodevelopment, musculoskeletal system, and metabolic diseases. The functions of B3GNTs represent the glycosylation of proteins is a crucial and frequently life-threatening step in progression of most diseases. In this review, we give an overview about the roles of B3GNTs in tumor, nervous system, musculoskeletal and metabolic diseases, describing the recent results about B3GNTs, in order to provide a research direction and exploration value for the prevention, diagnosis and treatment of these diseases.

The methodological quality of systematic reviews regarding the Core Outcome Set (COS) development

BackgroundThe Core Outcome Measures in Effectiveness Trials (COMET) working group proposed core outcome sets (COS) to address the heterogeneity in outcome measures in clinical studies. According to the recommendations of COMET, performing systematic reviews (SRs) usually was the first step for COS development. However, the SRs that serve as a basis for COS are not specifically appraised by organizations such as COMET regarding their quality. Here, we investigated the status of SRs related to development of COS and evaluated their methodological quality.MethodsWe conducted a search on PubMed to identify SRs related to COS development published from inception to May 2022. We qualitatively summarized the disease included in SR topics, and the studies included in the SRs. We evaluated the methodological quality of the SRs using AMSTAR 2.0 and compared the overall quality of SRs with and without protocols using the Mann-Whitney U test.ResultsWe included 175 SRs from 23 different countries or regions, and they mainly focused on five diseases: musculoskeletal system or connective tissue disease (n = 19, 10.86%), injury, poisoning, or certain other consequences of external causes (n = 18, 10.29%), digestive system disease (n = 16, 9.14%), nervous system disease (n = 15, 8.57%), and genitourinary system disease (n = 15, 8.57%). Although 88.00% of SRs included randomized controlled trials (RCTs), only a few SRs (23.38%) employed appropriate tools to assess the risk of bias in RCTs. The assessment results on the basis of AMSTAR 2.0 indicated that most SRs (93.71%) were rated as ‘’critically low’’ to ‘’low’’ in terms of overall confidence. The overall confidence of SRs with protocols was significantly higher than that without protocols (P <.001). Compared to the SRs with protocols on Core Outcome Measures in Effectiveness Trials (COMET), SRs with protocols on PROSPERO were of better overall confidence (P = .017).ConclusionThe overall quality of published SRs regarding COS development was poor. Our findings emphasize the need for researchers to carefully select the disease topic and strictly adhere to the requirements of optimal methodology when conducting a SR for the establishment of a COS.

Ethnobotanical study of Zhuang medicinal herbs of Ardisia: variety systematization, traditional uses, phytochemistry, pharmacology, clinical application, and toxicity.

This study aimed to systematize the Zhuang medicinal herbs of Ardisia (ZMHA) in China, to clarify the traditional use in Zhuang medicine and the dynamics of international research on phytochemistry, pharmacology, clinical application, and toxicity. There are 25 species of ZMHA, approximately 938 compounds from the different part, including triterpenoids, phenolics, volatile oils, etc. Pharmacological activity studies have also shown that this genus has anti-tumour, anti-inflammatory, anti-oxidant, anti-bacterial, anti-microbial, etc., and significant effects on respiratory, digestive, urinary, and musculoskeletal system diseases without toxic side effects. The Ardisia has a medicinal history of nearly a thousand years, mainly for treating diseases of the injuries, musculoskeletal, and symptomatic system in Zhuang medicine. Some plants, such as A. crenata, A. gigantifolia, and A. japonica, are also commonly used in folk Zhuang medicine formulas, to treat musculoskeletal, injury, respiratory, and urinate systems disease. These diseases are related to inflammation. These could provide a new direction for future new drug development research. Therefore, species identification and resource investigation should be strengthened, and conducted in vitro mechanism, in vivo pharmacology, clinical efficacy, and toxicology studies and establish a perfect quality standard system.

Understanding to Concept of Jarajanya Vyadhi (ageing related diseases) w.s.r. to Sandhigatavata (osteo arthritis)

As age advances Vata Dosha increases in an individual. This increasing Vata trigger and accelerates Dhatu Kshaya (depletion of tissues) and Bala Kshaya (diminished the body strength). Hence advancing age makes man prone to many diseases especially degenerative disorders. Asthis (bones) are the main seat of Vata Dosha and Sandhis (joints) is the main seat of Kapha Dosha. Vata Dosha is responsible for different body movements like Prasaarana and Akunchana of Sandhi (flexion and extension of joints), whereas Shleshaka Kapha located in Sandhi (joints) is provides nutrition, protection and to minimize the friction during the movement. Sandhigatavata is Vata pradhana vyadhi. It is quite similar to osteoarthritis, a degenerative joint disease stand in modern medical science. Sandhigatavata is Vata dominating disorder which result in inflammatory and degenerative changes in joints. Osteoarthritis is a disease of musculoskeletal system, affecting the joints mostly in elderly population and in weight bearing joints (particularly knee joint). The current standard modern medical pharmacological management of osteoarthritis includes the administration of analgesics and non-steroidal anti-inflammatory drugs (NSAIDs). However, their use neither provides adequate and significant relief in symptoms nor deceleration in disease process. Therefore, Vata shamaka aushadhis along with local application e.g. Abhyanga, different form of Swedana are beneficial. As Sandhigatavata is ageing related heath condition, Rasayana chikitsa can also be helpful in the management of Sandhigatavata (Osteoarthritis). This article enlighten the knowledge about Jarajanya vyadhi especially Sandhigatavata.

Polyphenols as Potential Antioxidants for the Treatment of Intervertebral Disc Degeneration.

Intervertebral disc degeneration (IDD) is a common musculoskeletal system disease, which is one of the most important causes of low back pain. Despite the high prevalence of IDD, current treatments are limited to relieving symptoms, and there are no effective therapeutic agents that can block or reverse the progression of IDD. Oxidative stress, the result of an imbalance between the production of reactive oxygen species (ROS) and clearance by the antioxidant defense system, plays an important role in the progression of IDD. Polyphenols are antioxidant compounds that can inhibit ROS production, which can scavenge free radicals, reduce hydrogen peroxide production, and inhibit lipid oxidation in nucleus pulposus (NP) cells and IDD animal models. In this review, we discussed the antioxidant effects of polyphenols and their regulatory role in different molecular pathways associated with the pathogenesis of IDD, as well as the limitations and future prospects of polyphenols as a potential treatment of IDD.

Respiratory Tract Diseases with Musculoskeletal System Interaction: A Scoping Review

Objective: Respiratory diseases and musculoskeletal disorders are significant causes of morbidity and mortality worldwide, especially among older adults and immunocompromised individuals. Although current guidelines encourage a multidimensional approach to diagnosis and treatment, the interaction between these disease categories has not been adequately investigated. This scoping review aims to provide an overview of current research on diseases related to both respiratory and musculoskeletal disorders and identify gaps for future studies.&#x0D; Methods: Using the PRISMA extension to scope the reviews, we focused on randomized controlled trials and cohort studies published in the last five years. A total of 2003 abstracts were identified in databases such as Scopus, WoS, PubMed, Medline, Cinahl, OpenAIRE and EBSCO. After removing 287 duplicates, 1716 articles were screened based on title and abstract. A total of 470 full-text articles were assessed for eligibility, resulting in 11 articles meeting our inclusion criteria.&#x0D; Results: The review shows limited but influential studies investigating the intersection between respiratory and musculoskeletal diseases. The findings suggest that musculoskeletal disorders may negatively affect respiratory functions and vice versa. Several studies have demonstrated potential biomarkers, the importance of sleep quality, and associations with multimorbidity.&#x0D; Conclusion: Although limited to 11 articles, this review highlights the importance of a more detailed understanding of the interactions between the musculoskeletal system and respiratory diseases. This may inform future diagnostic and treatment strategies. However, the limited number of studies in this area indicates that more research is needed, especially which contains interaction mechanism of musculoskeletal and pulmonary pathologies.

The potential role of stem cells-derived extracellular vesicles in the treatment of musculoskeletal system diseases.

The therapeutic potential of stem cells-derived extracellular vesicles (EVs)has shown a great progress in the regenerative medicine. EVsare rich in a variety of bioactive substances, which are important carriers of signal transmission and interactions between cells, and they play an important role in the processes of tissue repair and regeneration. Several studies have shown that stem cells-derived EVsregulate immunity, promote cell proliferation and differentiation, enhance bone and vascular regeneration, and play an increasingly important role in musculoskeletal system. This review aimed to describe the biological characteristics of stem cells-derived EVsand discuss their potential role in the therapy of musculoskeletal system diseases.

Temperature change between neighboring days and hospital admissions in China

The short-term temperature fluctuation caused by global climate change is one of the risk factors affecting public health. Exploring the association between temperature fluctuation and diseases, which has received relatively limited research attention, can contribute to enhancing awareness of health risks and taking timely measures for health risk alert and management. Therefore, this study aims to investigate the relationship between temperature change between neighboring days (TCN) and hospitalizations, identify diseases sensitive to extreme TCN, and evaluate the related disease burden. We collected meteorological and hospitalization data from 2014 to 2019 in 23 sites of China to explore the impact of TCN on hospitalizations. We first quantified site-specific associations between TCN and hospitalizations and then conducted meta-analysis to pool the results, to assess the relative risk of extreme TCN for susceptible diseases, and to estimate the related disease burden attributed to TCN. Stratified analyses were undertaken by age, sex, and disease type. Results showed that all-cause hospital admission was significantly linked to TCN. A negative TCN (below −1.9 °C) in the cool season and a positive TCN (above 1.0 °C) in the warm season increased the risk of hospitalization. People aged 15–64 years, men, and patients with musculoskeletal system or connective tissue diseases were more sensitive to extremely negative TCN during the cool season. People aged over 65 years, men, and patients with respiratory diseases were more sensitive to extremely positive TCN during the warm season. The attributable fraction to all-cause hospitalization from negative TCN in the cool season was 2.05% (95% CI: −0.90%, 4.53%) and from positive TCN in the warm season was 5.79% (95% CI: 2.98%, 8.31%). Circulatory diseases in the cool season and respiratory diseases in the warm season had the highest disease burden. Our findings indicate that awareness of TCN and its health risks should be promoted and evidence-informed policies are needed to reduce the risk of TCN.

An evaluation of pediatric emergency cases in the first year of the COVID-19 pandemic: A single-centre experience

AimNurses responsible for the care of children in the emergency department must be aware of the case profile of the pandemic era, better prepare for developing crises, and identify preventive services. The study aims to determine the pediatric emergency cases admitted during the pandemic and compare them to those during the pre-pandemic period. MethodsThe current study was designed as a single-center, retrospective, cross-sectional, and descriptive research. All patients aged between 0 and 18 who presented to the pediatric emergency department and had electronic patient records were included in the research population. The data were gathered over two periods: the pre-pandemic period from 2019 to 2020 and the pandemic period from 2020 to 2021. A total of 145,591 patient records were analyzed. ResultsThe number of admissions declined by 51.6% during the pandemic, and a noticeable difference in the reasons for emergency department visits (p = 0.001) was detected. The pandemic saw a decrease in the cases of respiratory, gastrointestinal diseases, and ear, nose, and throat problems. In contrast, there was an increase in the cases of musculoskeletal system diseases, infections, falls, traumas, and neonatal problems. ConclusionThe number of pediatric emergency cases decreased significantly during the pandemic, and compared to the pre-pandemic period, some changes in these cases were observed. Practical implicationsParticular attention should be given to ensuring that mothers of newborn infants receive adequate nursing care. Nurses working in the emergency department should keep their knowledge up-to-date regarding the changing health issues specific to the pandemic period.

Negative effects of the COVID-19 pandemic on the musculoskeletal system and depression in healthcare professionals.

During the COVID-19 pandemic, it is thought that healthcare workers are most exposed to musculoskeletal disorders. However, there are limited studies in the literature examining the musculoskeletal disorders among healthcare workers during the COVID-19 pandemic. The aim of this study was to examine musculoskeletal problems and psychological disorders in healthcare personnel working at Dicle University Faculty of Medicine and Research Hospitals during the COVID-19 pandemic and to come up with solutions for rehabilitation. The Cornell Musculoskeletal Disorder Questionnaire (CMDQ) was used to assess musculoskeletal problems in the past week. Additionally, Beck depression inventory (BDI) was used to evaluate the psychological state of the participants. Questions on demographic characteristics, habits, the presence and localisation of musculoskeletal system (MSS) diseases, severity and duration of pain, chronic diseases, trauma history and working conditions were included in the questionnaire prepared by the researchers. The study included 74 nurses, 42 residents, 26 specialists, 24 technicians, 16 dentists, 12 physiotherapists, and 26 other allied health personnel. Dentists had the highest total CMDQ score (160.73) whereas resident doctors had the lowest total CMDQ score (98.33). Low back, neck and back pain were the most common MSS problems. BDI was highest in nurses and 70.27% of the nurses were women. The total workplace ergonomics score was found to be 25.91%, and the visual analogue scale (VAS) was highest in nurses (6.72). While MSS pain is concentrated on the waist, neck and back regions in all healthcare workers. We concluded that the uncertainty associated with the pandemic, delay in preventive measures such as vaccination and medication, rapid and strong transmission of the disease and increasing number of deaths have led to an increase in stress, depression and burnout among healthcare workers.

Plasma-derived extracellular vesicle surface markers CD45, CD326 and CD56 correlate with the stage of osteoarthritis: a primary study of a novel and promising diagnostic tool of the disease

Recently, there is a growing interest in the research based on extracellular vesicles (EVs) which represent paracrine factors secreted by almost all cell types. Both, normal and pathological cells are able to release various types of EVs with different physiological properties, functions and compositions. EVs play an important role in intercellular communication, mechanism and tissue repair. Moreover, EVs could help not only in the treatment of diseases but also in their diagnostics. This work focused on the evaluation of the potential of EVs being used as biomarkers for the diagnosis of osteoarthritis (OA) based on a comparison of the composition of EVs separated from platelet-poor plasma (PPP) of healthy donors and OA patients at different stages of OA. OA is established as a complex syndrome with extensive impact on multiple tissues within the synovial joint. It is a chronic disease of musculoskeletal system that mainly affects the elderly. Depending on the use of the Kellgren–Lawrence classification system, there are four grades of OA which have a negative impact on patients' quality of life. It is very difficult to detect OA in its early stages, so it is necessary to find a new diagnostic method for its timely detection. PPP samples were prepared from whole blood. PPP-EVs were separated from 3 groups of donors—healthy control, early stage OA, end-stage OA, and their content was compared and correlated. EVs from PPP were separated by size exclusion chromatography and characterized in terms of their size, yield and purity by NTA, western blotting, ELISA and flow cytometry. Detection of surface markers expression in EVs was performed using MACSPlex approach. Inflammatory and growth factors in EVs were analysed using MAGPix technology. Our study confirmed significant differences between EVs surface markers of patients and healthy controls correlating with the age of donor (CD63, CD31 and ROR1) and stage of OA (CD45, CD326 and CD56), respectively. Circulating EVs have been under extensive investigation for their capability to predict OA pathology diagnosis as potential targets for biomarker discovery. Taken together, obtained results indicated that PPP-EVs surface markers could be used as potential biomarkers in the early diagnosis of OA.

State of health of Russian adolescents

The goal is to assess the state of health in 15–17 years old adolescents and justify the main medical and social directions for its protection.&#x0D; Materials and methods. An analysis based on the official statistics data in morbidity, disability, and mortality of the adolescent population in the Russian Federation for the period 2012–2022 was carried out. &#x0D; Results. It has been noted a stagnation of the level of general and primary morbidity in adolescents, in the structure of which the majority were respiratory diseases. Among the general morbidity causes the first places were occupied by diseases of eyes, musculoskeletal system, injuries, poisoning, and other external causes, among the primary morbidity causes are injuries, poisoning and other external causes, diseases of the skin and subcutaneous tissue, eyes. There was a significant reduction in the incidence of socially significant diseases (sexually transmitted infections, mental disorders associated with alcohol consumption, drug addiction, substance abuse, tuberculosis). The prevalence of disability in adolescents significantly exceeds that of the entire child population, and has a steady upward trend. In the structure of the general disability causes the first rank places were occupied by mental disorders and disorders of behaviour, diseases of the nervous, endocrine systems, and congenital anomalies. There is a significant decrease in teenage deaths, but at a pace slower than in the child population as a whole. In the structure of the main causes of mortality in 15–17 years children the vast majority were injuries, poisoning and other external causes, among which cases of damage with uncertain intentions, suicide, road accidents, drowning, and poisoning dominated.&#x0D; Conclusion. The problem of preserving and promoting the health and life of adolescents requires an integrated approach to its solution within the framework of special federal and regional programs. It is necessary to carry out targeted coordinated actions of federal executive bodies, services of the state and municipal health care system, social protection and education, executive authorities of the subjects of the Federation, local governments, scientific, and public organizations.

ОСОБЕННОСТИ ДИНАМИКИ И СТРУКТУРЫ ПРОФЕССИОНАЛЬНОЙ ЗАБОЛЕВАЕМОСТИ В РЕСПУБЛИКЕ ТАТАРСТАН

Introduction. In their labor activities, employees are exposed to some hazardous and harmful factors of the working environment, of which the key ones are physical factors, such as noise or local/general vibration; physical and functional overloads of the musculoskeletal system; aerosols of predominantly fibrogenic action; and dust. The core indicator characterizing the occupational risk of a health problems is occupational morbidity. Aim. Studying the time profile and structure of occupational morbidity in employees of the Republic of Tatarstan. Material and methods. Occupational morbidity over the years 2007-2021 was analyzed. Over the period under research, occupational illnesses were diagnosed in 2,546 employees of 130 occupational categories. Results and discussion. The highest rates of occupational morbidity were recorded among employees of agricultural and mechanical engineering enterprises and companies. About 60% of occupational diseases were diagnosed in those pursuing 7 professions. What stands out is the similarity of occupational pathologies in assembly workers and riveters, fettlers, and farm-machine operators, who were diagnosed with vibration syndrome and occupational deafness. Occupational diseases of respiratory and musculoskeletal systems were additionally diagnosed in fettlers. In milkmaids, the occupational diseases of musculoskeletal system take the leading place. Polymorbidity of occupational pathology is most pronounced in machine milking operators followed by tractor drivers and fettlers. Conclusion. It is necessary to ensure early occupational pathology diagnoses in routine health screenings and timely referrals to the occupational pathology center in order to perform causal relationship assessments between disease and occupation.