Murrah Buffalo Heifers Research Articles

Relationship of external genitalia measurement with antral follicular count and estrus induction response in pre-pubertal Murrah buffalo heifers

Relationship of external genitalia measurement with antral follicular count and estrus induction response in pre-pubertal Murrah buffalo heifers

Comparative antimicrobial activity in Murrah Buffalo (Bubalus Bubalis) Heifers’ urine and urine distillate

Comparative antimicrobial activity in Murrah Buffalo (Bubalus Bubalis) Heifers’ urine and urine distillate

Heat ameliorative measures in Murrah buffalo (Bubalus bubalis) heifers during summer: effect on microclimate, thermal comfort, and behavior.

Water buffalo (Bubalus bubalis) is the mainstay of milk production in Asian countries including India. However, the hot climate of the country remains the biggest bottleneck to exploit the potential of this species. Therefore, a study was conducted to assess the effect of heat ameliorative measures on microclimate, thermal comfort, and behavior of Murrah buffalo heifers during summer. Buffalo heifers (n = 24) between the age of 15 and 20months with a mean body weight of 363.75 ± 11.27kg were randomly grouped into four treatments based on their age and body weight. The heifers kept in the shed without any cooling served as CON (control), while the animals of group CJ were tied with a cooling jacket. The buffalo heifers of the CJF group were subjected to the cooling jacket with forced air ventilation, whereas the intermittent (10min at 2-h intervals) sprinkling followed by forced air ventilation was practiced in group SF between 900 and 1800h throughout the experiment. The microclimatic variables were low in the sheds of groups CJ, CJF, and SF than the CON. The physiological responses such as rectal temperature, skin temperature, respiration rate, and pulse rate were reduced in groups CJ, CJF, and SF than the CON at 1400h. The serum cortisol was less in the CJF and SF than those of CON group. The animals of the CJ, CJF, and SF groups spent more time for feeding and rumination with less frequent longer bouts. The extended periods of lying followed by shorter standing and idling were observed in groups CJ, CJF, and SF when compared with the CON. It may be therefore concluded that cooling jacket and intermittent sprinkling in combination with forced air ventilation might be helpful in improving the micro-environment, thermal comfort, and behavior of buffalo heifers particularly under tropical climates during summer.

Functional Genes in Relation to Residual Feed Intake in Murrah Buffalo Heifers

Functional Genes in Relation to Residual Feed Intake in Murrah Buffalo Heifers

Effect of feeding concentrate mixtures containing varying levels of dried moringa (Moringa oleifera) leaves on haematobiochemical and sex hormonal parameters in Murrah buffalo heifers

A feeding trial was conducted for a period of 120 days for studying the haemato-biochemical and sex hormonal parameters in buffalo heifers fed with concentrate mixtures containing varying levels of dried moringa (Moringa oleifera) leaves. Fifteen Murrah buffalo heifers aged 18-26 months were selected and randomly allotted to three groups of five animals each and fed three dietary treatments, containing concentrate mixture (20 per cent CP and 68 per cent TDN) and ad libitum green grass. Dried moringa leaves were incorporated at 0, 10 and 20 per cent in the concentrate mixture for treatments T1 (control), T2 and T3 respectively. Comparison of serum biochemical values revealed higher serum glucose and oestrogen in buffalo heifers fed T3 when compared to those fed T1 and T2. The serum cholesterol values were significantly lower (p<0.05) for buffalo heifers fed T2 and T3 when compared to T1. Evaluation of the results obtained in the present study revealed that moringa leaves can be effectively incorporated up to 20 per cent in the concentrate mixture of buffalo heifers without any adverse effects on haemato-biochemical parameters with improved oestrogen levels indicating better reproductive performance.

Impact of incorporating Asparagus racemosus (Shatavari) into the diet on the growth performance of Murrah buffalo heifers (Bubalus bubalis)

Impact of incorporating Asparagus racemosus (Shatavari) into the diet on the growth performance of Murrah buffalo heifers (Bubalus bubalis)

Effect of lowering blood cortisol level along with progesterone priming on intensifying the secondary symptoms of oestrus in Murrah buffaloes during the hot summer months.

The present study sought to intensify oestrus symptoms in heat-stressed pre-pubertal Murrah buffalo heifers. The first experiment aimed at lowering the blood cortisol level. Twenty pre-pubertal buffalo heifers approximately 36-40months of age were randomly allocated to four groups of five buffaloes. Group Gly received 100 ml glycerol orally for 8 days (day 0 is the start of treatments), group Gly-E received intra-muscular injections of 500 mg tocopherol (vitamin E activity) and 150 mg sodium selenite on alternate days (four treatments) in addition to treatments received by group Gly, group Gly-E + B-Ch received intra-muscular injections of vitamin B-complex and choline daily for 8 days in addition to the treatments received by group Gly-E. The control group Co-1 did not received any treatments. Blood cortisol and glucose level was measured on day 7 post-treatment. The second experiment sought to test the effect of lowering the blood cortisol level and progesterone priming on the intensity of oestrus symptoms in heat-stressed pre-pubertal buffalo heifers. Eighty buffaloes were allocated into four groups of twenty buffaloes. P4 group received intra-vaginal progesterone CIDR for 5 days (day 0 is the day of start of treatment). Gly-E group received the same treatment as in the first experiment for 7 days (day 0-6) and P4 + Gly-E received the combination of P4 and Gly-E groups. The control group Co-2 did not receive any treatments; however, on day 6, all groups received intra-muscular injection of 2mg estradiol benzoate. The buffaloes were graded for oestrus symptoms on the basis of mucus discharge (A-scanty and B-copious), vulvar redness (slightly pink and moist-A and deep red and moist-B), ferning (nil or poor-A and good with arborization-B) and cellularity of cervical mucus (Type A goblet cells with round nucleus and abundant cytoplasm and Type B goblet cells with cylindrical nucleus and scanty cytoplasm). The blood glucose level (mean ± SEM) of Gly group on day 7 of treatment in experiment 1 was 58.3 ± 0.4mg/dl that was significantly greater than Co-1 (52.4 ± 0.4mg/dl), Gly-E (53.5 ± 0.8mg/dl) and Gly-E + B-Ch (52.7 ± 0.6mg/dl) groups; however, the difference was found to be non-significant among Co-1, Gly-E and Gly-E + B-Ch groups. The cortisol level (mean ± SEM) on day 7 in groups Gly (11.4 ± 0.6ng/ml), Gly-E (8.1 ± 0.5ng/ml) and Gly-E + B-Ch (7.5 ± 0.4ng/ml) was significantly lower than groups Co-1 (15.5 ± 0.3ng/ml), though the difference between groups Gly-E and Gly-E + B-Ch was non-significant and the level in group Gly-E significantly lower than Gly. Thus, the combination of vitamin E and selenium along with glycerol had the best effect in the reduction of blood cortisol level in heat-stressed buffaloes. In the second experiment the blood estradiol level on day 7 in all groups was around 40 pg/ml. Cortisol reduction (group Gly-E) improved oestrus symptoms (mucus discharge and ferning) compared to control, but when compared to group P4 showed non-significant difference with respect to mucus discharge, vulvar redness and ferning of cervical mucus. However, when cortisol reduction treatments were combined with progesterone priming (group P4 + Gly-E) oestrus symptoms were improved with respect to mucus discharge and ferning thus, combination treatment had synergistic effect. This effect was also observed with cellularity of cervical mucus, where combination treatment group P4 + Gly-E produced cervical mucus with significantly lower percentage of Type B cells compared to group P4 and group Gly-E. Thus, administration glycerol and vitamin E selenium combination reduces blood cortisol level and along with progesterone priming can be effective to improve the oestrus symptoms of buffaloes during the hot summer months.

Microclimate modification in Murrah buffalo (Bubalus bubalis) heifers during summer: Effect on intake, growth and hematobiochemistry.

The present investigation evaluated the effect of microclimate modification on feed intake, growth performance, and hemato-biochemical profile of Murrah buffalo (Bubalus bubalis) heifers during summer. Twenty-four buffalo heifers, between 15 and 20 months of age with an average body weight of 363.75±11.27kg, were randomly assigned to 4 groups based on their age and body weight. The heifers of the T0 (control) group were kept under the shed without any cooling treatment, while the animals in group T1 were tied with a cooling jacket. Buffalo heifers of group T2 were subjected to a cooling jacket with forced ventilation and animals in group T3 were treated with intermittent sprinkling (10min., at 2h intervals) and forced ventilation between 9.00 AM and 6.00 PM during the experiment. The ambient temperature inside the shed and core body temperature were reduced in groups T1, T2, and T3 compared to T0. Heifers had higher dry matter intake when subjected to cooling treatments T1, T2, and T3, whereas water intake was reduced in T2 and T3 groups. The animals in T2 and T3 groups attained higher average daily weight gain, while the feed conversion was better in the T3 group compared to T0. The hematological measures such as hemoglobin, total erythrocyte count, and total leucocyte count were found higher in T0. The serum glucose, sodium, and potassium levels increasedand alkaline phosphatase activity decreased in groups T1, T2 and T3 when compared with T0. It can be concluded that the provision of intermittent sprinkling and cooling jacket in combination with forced ventilation could improve the microclimate, which in turn could enhance the performance of Murrah heifers during hot summer days in the tropics.

Effect of Astaxanthin and Copper Supplementation on Growth, Immunity, Antioxidant, and Blood Biochemical Status of Growing Murrah Buffalo Heifers.

This study was aimed to explore the effect of astaxanthin (ASTX) and copper (Cu) supplementation on the growth, immunity, antioxidant, and blood biochemical status of growing Murrah buffalo heifers. Twenty-eight Murrah buffalo heifers were selected and randomly divided into 4 groups (n = 7) after blocking by body weight (BW) (129.86 ± 5.37kg) and age (9.05 ± 1.02months). The heifers were fed basal total mixed ration diet without supplementation (CON) or with ASTX (0.20mg/kg BW; AX), Cu (10mg/kg DM; CU), or ASTX + Cu (0.20mg/kg BW + 10mg/kg DM; AX + CU) for 90days of study period. The result showed that BW and dry matter intake (DMI) were significantly higher (P < 0.05) in AX + CU than that in other groups. The average daily gain (ADG) and feed conversion efficiency (FCE) were statistically higher (P < 0.05) in treatments than the values observed in CON. The feed conversion ratio (FCR) was reported significantly lower (P < 0.05) in the AX + CU group followed by AX, CU, and CON groups. The total leukocytes count (TLC), lymphocytes, and total immunoglobulin (TIG) were statistically higher (P < 0.05) in AX + CU groups than that found in other groups. However, neutrophil % decreased (P < 0.05) in the AX + CU group than its level in other groups. Superoxide dismutase (SOD), catalase (CAT), and total antioxidant (TAA) levels were observed higher (P < 0.05) in treatments supplemented with ASTX, Cu, or both than CON group. Thiobarbituric acid reactive substance (TBARS) concentration was lower (P < 0.05) in treatments than its level found in the CON group. Glucose level was higher (P < 0.05); however, non-esterifies fatty acid (NEFA) was lower (P < 0.05) in AX + CU than that in others groups. The level of cholesterol (CH), HDL cholesterol (HDL-CH), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were reported lower (P < 0.05) in the AX + CU group followed by CU, AX, and CON groups. The copper (Cu) level was higher (P < 0.05) in CU and AX + CU than AX and CON groups. The result of the present study indicated that the supplementation of ASTX, Cu alone, or their combination improved the growth, immunity, antioxidant status, and liver function of growing heifers.

Differential expression of miRNAs and related mRNAs during heat stress in buffalo heifers

The present experiment was aimed to study differential expression of miRNAs and related mRNAs during heat stress (HS) in buffalo heifers. Twelve Murrah buffalo heifers aged between 1.5 and 2.0 years, weighting between 250 and 300Kg were randomly assigned into two equal groups. The animals were kept in the psychrometric chamber under Thermo-neutral (TN; THI=72) and HS (THI=87-90) conditions for 6h every day between 1000 and 1600h for 21 days. The blood sampling was done at 1500h on 15th day of the experiment and physiological parameters viz. pulse rate (PR), respiratory rate (RR) and rectal temperature (RT) were recorded at 1500h on day -5, -3, -1, 0, +1, +3, +5 with respect to blood sampling. PBMCs were used for extraction of miRNAs and total RNA; and first strand cDNA was synthesized. qPCR was performed for relative gene expression studies. Physiological, hematological (erythrocytic indices), biochemical (triglycerides, urea, ALT, AST, LDH), redox (SOD, ROS) and endocrine parameters (T4) altered significantly (P<0.05) during HS as compared to TN. Out of eight targeted miRNAs only four were expressed in buffalo heifers. The relative expression of bta-mir-142, bta-mir-1248 and bta-mir-2332 was significantly (P<0.05) up-regulated whereas expression of bta-mir-2478 was significantly (P<0.05) down-regulated during HS as compared to TN. The relative expression of the predicted target genes i.e. HSF1, HSP60, HSP70, HSPA8 and HSP90 were significantly (P<0.05) up-regulated whereas HSF4 expression was significantly (P<0.05) down-regulated during HS as compared to TN. It can be concluded that a THI of 87-90 could lead to a moderate HS in buffalo heifers. Differential expression studies of miRNAs and related mRNAs in present study deciphers the role of miRNAs in the heat tolerance in buffalo heifers.

Higher antral follicular count is associated with body weight in peri-pubertal Murrah buffalo heifers

AMH had no correlation with AFC and BW, but AFC was correlated with BW in high antral follicular group heifers. Furthermore, this study shows that AMH exhibited a trend with AFC in peri-pubertal buffalo heifers.

Environmental factors affecting reproductive performance of Murrah buffaloes

ABSTRACT Buffalo is the backbone of the farmer’s economy in Indian dairy industry. Data from 288 Murrah buffalo female calves maintained at the Livestock Research Center, Karnal, Haryana, born in the period between 2000 and 2009 were used for this study. Data were analysed using least-square procedures. Results indicated that season and period of birth had significant (P < 0.05) effect on reproductive performance of Murrah buffalo heifers. However, there was no significant effect of birth weight on different reproductive performance. The phenotypic parameter estimates indicated that the season and birth weight of a calf can be used as a selection tool for improvement of reproductive traits considering its high positive phenotypic correlations with succeeding reproductive traits.

Effect of multi-nutrient liquid supplement on serum minerals and hormone profile in buffalo heifers

Eighteen Murrah buffalo heifers (average BW of 290±2.5 kg) were randomly distributed into three groups of six each in an experiment based on randomized block design to investigate the effect of partial replacement of concentrate mixture with molasses based multi-nutrient liquid supplements (MMLS) on serum mineral and reproductive hormonal profile. During 240 d of experimental period, all animals were supplied with green forages (2 kg DM/d) and wheat straw ad libitum. Animals in group T1 (control) were fed a concentrate mixture (maize 40, wheat bran 40, soyabean meal 17, mineral mixture 2, and salt 1%) to meet their nutrient requirements. However, 20% of the concentrates were replaced with MMLS and MMLS plus in groups T2 and T3, respectively. Blood was collected on d 0, 120 and 240 days of experiment to evaluate serum mineral and reproductive hormonal (progesterone and estradiol) profile. Serum concentrations of Ca, P, Fe and Mn were similar among the groups. Serum concentration of Cu and Zn was higher in groups T2 and T3. Concentrations of progesterone and estradiol were similar among the groups, but showed an increasing trend on day 240. It was concluded that partial replacement of concentrate mixture with MMLS and MMLS plus improved Cu and Zn status without any adverse impact on other minerals and reproductive hormone profile.

English

English

Effects of melatonin supplementation prior to Ovsynch protocol on ovarian activity and conception rates in anestrous Murrah buffalo heifers during out of breeding season

Buffalo heifers have tendency to show anestrus during summer season. Melatonin has been used for correcting summer dependent anestrous via inducing resumption of ovarian activity. Therefore, the investigation was conducted to compare efficacy of melatonin for induction of estrus and conception rate with Ovsynch protocol in summer anestrous Murrah buffalo heifers. Thirty, summer anestrous Murrah buffalo heifers were selected and divided into two groups- treatment (n = 20; 12 melatonin implants) and control (n = 10; no treatment). On day 28 post-implant insertion, animals of both the groups were subjected to Ovsynch protocol. Blood sampling and ovarian ultrasonography were conducted to measure plasma melatonin, progesterone concentration and ovarian dynamics, respectively. No animal in either group showed estrus during first 28 days post-implant insertion. However, estrus induction rate was 100% after Ovsynch protocol in both groups. As compared to controls, treatment group exhibited higher (p < 0.05) plasma melatonin on days 1, 4, 8, 15, 22 and 28 post-melatonin, with highest concentration on day 4. The progesterone concentration increased (p < 0.05) on days 15 and 22 post-melatonin treatment. The treatment group had larger (p < 0.05) preovulatory follicle on day of AI, subsequently developed larger (p < 0.05) corpus luteum and higher plasma progesterone concentrations by day 12 post-AI as compared to the control group. The overall conception rate was 50 and 20% in treatment and control groups, respectively. In conclusion, melatonin treatment along with Ovsynch protocol improved the luteal profiles as well as the conception rate in buffalo heifers when compared with animals treated with Ovsynch protocol alone during summer season.

Quantification of methane emissions from Murrah buffaloes fed different energy diets during various temperature humidity index periods in a tropical environment

The objective of the present study was to quantify the enteric methane emission in Murrah buffalo heifers at high (summer) and low (winter) temperature humidity index (THI) period fed different energy level diets. Thirty-six growing Murrah buffalo heifers of average bodyweight (158.51 ± 16.5 kg) were distributed into three groups of six animals each separated based on their bodyweight and fed for the period of 120 days each during summer (high THI, 78–85) and winter (low THI, 50–61). The animals were fed on three different levels of metabolisable energy (ME) content and the Control ration (T1) having ME content according to ICAR (2013) and T2 and T3 were having 115% and 85% ME than the Control respectively, in total mixed-based ration. The SF6 tracer gas technique was used to quantify the enteric methane emission by the animals. Methane emission (g/day) of Control and the high energy (T1 and T2) group was lower (P &amp;lt; 0.05) than the low energy (T3) fed group in both seasons. Methane losses as percentage of gross energy intake was lower (P &amp;lt; 0.01) during the winter season. However, in the low energy treatment group (T3) at both seasons these values are higher than the IPCC recommended value (6.5%) for calculation of national inventory of greenhouse gas emission from enteric sources. In between season average daily gain (kg) was higher (P &amp;lt; 0.01) in the winter season and among the treatment groups it was higher (P &amp;lt; 0.01) in the high energy group (T1, T2). Higher (P &amp;lt; 0.01) digestibility of dry matter, organic matter, neutral detergent fibre and acid detergent fibre was reported in the Control and high energy-fed group. Whereas in the summer season digestibility of dry matter, organic matter, crude protein and acid detergent fibre was higher (P &amp;lt; 0.01) than in the winter season. It can be concluded that energy levels significantly (P &amp;lt; 0.05) affect methane emissions and was lower in the Control and high energy-fed group (T1 and T2). However, while quantifying methane emission in changing THI period at different seasons it did not show any significant variation.

Buffalo heifers selected for lower residual feed intake have lower feed intake, better dietary nitrogen utilisation and reduced enteric methane production.

This study was conducted to evaluate the utilisation of the residual feed intake (RFI) as a feed efficiency selection tool and its relationship with methane emissions. Eighteen Murrah buffalo (Bubalus bubalis) heifers were fed ad libitum with total mixed ration (TMR) for 120days. Based on linear regression models involving dry matter intake (DMI), average daily gain (ADG) and mid-test metabolic body size (MBW0.75 ), heifers were assigned into low and high RFI groups. The RFI varied from -0.09 to +0.12kg DM/day with average RFI of -0.05 and 0.05kg DM/day in low and high RFI heifers respectively. Low RFI heifers ate 11.6% less DM each day, yet average daily gain (ADG) and feed utilisation were comparable among low and high RFI groups. Low RFI heifers required significantly (p<.05) less metabolizable energy for maintenance (MEm) compared to high RFI heifers. Apparent nutrient digestibility showed non-significant difference (p>.05) among low and high RFI groups. Although the nitrogen balance was similar among heifers of low and high RFI groups, nitrogen metabolism was significantly higher (p>.05) in high RFI heifers. Comparison of data from heifers exhibiting the low (n=9) and high (n=9) RFI showed that the low RFI heifers have lower enteric methane production and methane losses than high RFI heifers. In conclusion, results of this study revealed that selection of more efficient buffalo heifers has multiple benefits, such as decreased feed intake and less emission of methane.

&lt;b&gt;Chemical-physical characteristics of buffalo (&lt;i&gt;Bubalus bubalis&lt;/i&gt;) meat subjected to different aging times

The aim of this study was to evaluate the effects of different aging times on the meat characteristics from young Murrah buffaloes slaughtered at 20–24 months of age (experiment I; n=10) and Murrah buffalo heifers slaughtered at 32-36 months of age (experiment II; n=10), with the purpose of determining the best aging time to tenderize meat from both experiments. Samples of the longissimus thoracis muscle from buffaloes slaughtered at each age were aged for 7, 14, and 21 days (0±1°C). After this period, analysis of cholesterol, pH, cooking loss, shear force, myofibrillar fragmentation index, meat color, and fatty acid profile was performed. Aging greatly improved the tenderness (p &lt; 0.05) but caused a change in color (p &lt; 0.05), which, even without difference between the treatments for subjective perception of the color (ΔE; p &gt; 0.05), was considered very noticeable to the human eye. Furthermore, aging reduced the polyunsaturated fatty acids (p &lt;0.05) and did not affect the levels of cholesterol (p &gt; 0.05) in the meat of young buffalo and buffalo heifers of advanced age. Based on the evaluated meat characteristics, concludes that seven days of aging are sufficient for older buffalo heifers, but 21 days of aging are required for the younger male group.

Residual Feed Intake, Digestibility of Nutrients and Efficiency of Water Utilizations in Murrah Buffalo Heifers

A metabolism trial was conducted for d to determine residual feed intake RFI measure of the difference between actual feed intake and expected feed requirement dry matter intake DMI digestibility and metabolism of nutrients at the end of a days d of a feeding trial with Murrah buffalo heifers kg plusmn and plusmn d of age Preliminary selection of heifers as efficient and less efficient was done by regressing actual DMI on predicted DMI g head d using the model DMI g kg BW predicted DMI g kg BW and RFI categories were sorted out by regressing mean DMI for the days feeding period on BWG and mid test MBW using the model Yi BWG g d mid test MBW kg ei where Yi DMI regression intercept and regression coefficients on BWG and mid test MBW respectively and ei uncontrolled error or RFI heifers with ve RFI values are less efficient and those with ndash ve RFI values efficient Differences were not statistically significant The actual dry matter DM crude protein CP digestible organic matter DOM and total digestible nutrient TDN intakes of RFI I heifers plusmn plusmn plusmn and plusmn kg head d respectively were not different from those of RFI II plusmn plusmn plusmn and plusmn kg head d respectively RFI II heifers digested DM OM and NDF plusmn plusmn and plusmn respectively better than those of RFI I heifers plusmn plusmn and plusmn respectively and thus RFI II had higher N balance plusmn head d than that of RFI I heifers plusmn g head d Water loss per unit of water intake WI in feces of RFI II plusmn was lower than that of RFI I heifers plusmn and improved amount of water available for RFI II for metabolism as a result of which water loss in urine proportionally increased in RFI II plusmn WI than in RFI I heifers plusmn WI Water loss per unit of DMI in feces plusmn vs plusmn DMI and urine plusmn vs plusmn DMI for RFI I vs RFI II respectively followed similar trend as that of water loss per unit of WI Mainly because of small sample size differences in nutrient intakes between RFI I and RFI II heifers were not significant however RFI II with similar feed intake had higher N balance digestibility of nutrients and level of water utilization and could be selected as being more feed efficient heifers

Anti-Müllerian hormone and antral follicular count in early and delayed pubertal Murrah buffalo heifers

The present study was designed to determine the relationship between Anti-Müllerian hormone (AMH), antral follicular count (AFC) and body weight (BW) in early and delayed pubertal buffalo heifers. Out of 72 heifers screened ultrasonographically, 19 heifers were selected based on age, BW and attainment of puberty for the present investigation. They were divided into two groups (Group I, n=12; Group II, n=7). Group I heifers (age: 30–40 months; BW: 370–468kg) showed no signs of puberty whereas Group II heifers (age: 18–29 months, BW: 279–318kg) attained puberty. Total number of all visible antral follicles >1mm were counted and cyclicity status was monitored at monthly interval. Single blood sample was collected for estimation of serum AMH concentration once the heifers were adjudged cyclic by the presence of corpus luteum. We found lower AMH concentration in delayed pubertal heifers (0.099±0.006ng/ml) as compared to early pubertal heifers (0.17±0.03ng/ml) but, difference was not significant (p=0.0788). Likewise, AMH showed non-significant correlation with AFC and BW in both groups. But, there was significant relationship between BW and AFC in delayed (r=0.79; p=0.00) and early (r=0.86; p=0.01) pubertal heifers. In conclusion, this study indicates non-significant difference between AMH and AFC in early and delayed pubertal heifers. Moreover, AMH had no correlation with AFC and BW, but BW was highly correlated with AFC in both study groups.