Multipurpose Utilization Research Articles

Silk Proteins: Designs from Nature with Multipurpose Utility and Infinite Future Possibilities.

This is a Perspective on nature as a story-teller, where inputs of evolution drove the remarkable protein designs found in silks. This selection process has resulted in silk materials with novel chemistry and properties to support organism survival in nature, yet with newfound utility in everything from comic books and automobiles to medicine. With growing global concerns related to environmental health, silks also serve as an invaluable instructional guide to the future of sustainable material designs.

The formation of nicotine heterosis is mainly achieved by enhancing the nicotine transport capacity in hybrids

Nicotine exhibits obvious heterosis, which can be used to create Nicotiana tabacum L. (tobacco) varieties with varying nicotine content. However, the reasons for the formation of nicotine heterosis and its relationship to nicotine transport and accumulation remain unknown. This study conducted a comprehensive analysis of six tobacco hybrids with varying heterosis levels and their parent materials from various aspects, such as phenotype, physiology, and transcriptomics. The results showed that the direct path coefficient of transport heterosis to nicotine heterosis was highest in hybrids, at 0.98, and a highly significant positive correlation between the two. The plant height, thick stalk circumference, large flow of tissue fluid in the stalk, and high nicotine concentration of tobacco were the underlying factors that led to the strong nicotine transport capacity of hybrids. The formation of nicotine transport heterosis in hybrids was mainly influenced by non-additive gene effects (accounting for 89.93%), with over-dominant effects playing a dominant role (accounting for 58.79%). Among non-additive expression DEGs, nicotine transporter related multi antimicrobial extrusion protein, drug/metabolite transporter, ABC family transporter, and glutathione S-transferase were significantly upregulated in hybrid strains. The RT-qPCR results indicated that these genes related nicotine transport also exhibited heterosis at the expression level. Our results revealed that the formation of nicotine heterosis is mainly achieved by enhancing the nicotine transport capacity in hybrids. The results are not only beneficial for promoting the theoretical study of nicotine heterosis in tobacco and the breeding and utilization of hybrids, but are also of great significance for guiding nicotine production and promoting its multipurpose utilization.

GDF-15 improves the predictive capacity of Steatotic liver disease non-invasive tests for incident morbidity and mortality risk for cardio-renal-metabolic diseases and malignancies

Background & aimsNoninvasive tools (NITs) are currently used to stratify the risk of having or developing hepatic steatosis or fibrosis. Their performance and a proteomic-enabled improvement in forecasting long-term cardio-renal-metabolic morbidity, malignancies, as well as cause-specific and all-cause mortality, are lacking. Therefore, the performance of established NITs needs to be investigated in identifying cardio-renal-metabolic morbidity, malignancies, cause-specific and overall mortality and improve their performance with novel, proteomic-enabled NITs, including growth differentiation factor 15 (GDF-15), allowing multipurpose utilization. Methods502,359 UK Biobank participants free of the study outcomes at baseline with a 14-year median follow-up were grouped into three categories: a) general population, b) potentially metabolic dysfunction-associated steatotic liver disease (MASLD) population, c) individuals with type 2 diabetes mellitus. The investigated NITs include Aspartate aminotransferase to Platelet Ratio Index (APRI), Fibrosis 4 Index (FIB-4), Fatty Liver Index (FLI), Hepatic Steatosis Index (HSI), Lipid Accumulation Product (LAP), and metabolic dysfunction–associated fibrosis (MAF-5) score. ResultsAdding GDF-15 to the existing NITs led to significantly increased prognostic performance compared to the traditional NITs in almost all instances, reaching substantially high C-indices, ranging between 0.601 and 0.808, with an overall >0.2 improvement in C-index. Overall, with the GDF-15 enhanced NITs, up to more than seven times fewer individuals need to be screened to identify more incident cases of adverse outcomes compared to the traditional NITs. The cumulative incidence of all outcomes, based on the continuous value percentiles of NITs, is increasing exponentially in the upper quintile of the GDF-15 enhanced NITs. ConclusionsThe herein-developed GDF-15 enhanced indices demonstrate higher screening effectiveness and significantly improved prognostic abilities, which are reduced to practice through an easy-to-use web-based calculator tool (https://clinicalpredictor.shinyapps.io/multimorbidity-mortality-risk/).

Study on Maturity Indices and Effect of Growing Substrates on Seed Germination of Litsea glutinosa

Aim: Litsea glutinosa is a multipurpose, fast-growing tree widely exploited due to its multipurpose utilisation. Species reported low seed viability, hence generally propagated through vegetative methods. We studied the maturity indices of fruits and seeds to determine the optimal collection stage. Studied the effect of different growing substrates and Gibberellic acid (GA3) treatment on the germination percent (GP), mean germination time (MGT), germination value (GV), peak value (PV), and germination index (GI). Study Design: Study was conducted at Forest Tree Seed Laboratory, ICFRE-FRI. The change in fruit colour from green, dark brown purple, and dark blue stages was compared with the seed parameters, germination percent, mean germination time, germination value, etc. to obtain the most suitable stage for fruit and seed maturity. Seed germination experiment was conducted in Complete Randomized Design with four replications of 25 seeds for each treatment. Seeds were sown directly on different germination substrates, such as filter paper, sand, and vermiculite, without treatment and also with 0.05% GA3 pretreatment. Seeds were kept in the seed germinator at 25±1ºC with a 24-hour photoperiod and high relative humidity (RH > 90%). Results: The highest germination rate was observed in vermiculite with GA3 soaking . i.e., 80±3.26%, followed by vermiculite 68±8.64%. The lowest germination percent of 5±4.08% was observed in seeds plated on filter paper with GA3 soaking. No germination was recorded in the seeds plated on the filter paper. Conclusion: Most suitable germination medium to maximise the germination of L. glutinosa seed is vermiculite. Furthermore, the pretreatment of seeds in GA3 (0.05%) for 24 hours and sowing in vermiculite improves overall performance with respect to GP, MGT, GV, PV, and GI. In the context of maturity indices, the dark blue stage with a moisture content of around 50% is the right stage for the collection of seeds of the species. This stage is characterised by high GP, GV, and low MGT values as compared to other growth stages.

The multipurpose utilization of inorganic materials in adsorption treatment of wastewater and core-modification of color index pigment red 57:1

Purpose This study aims to realize the multipurpose use of inorganic materials in adsorption treatment of pigment wastewater and preparation of core-modified Color Index Pigment Red 57:1 (C.I. Pigment Red 57:1, PR 57:1). Design/methodology/approach In this paper, the inorganic materials (sepiolite and SiO2·nH2O) were used in both PR 57:1 production wastewater treatment and its core-modification. The inorganic material firstly adsorbed 3-hydroxy-2-naphthoic acid (bon acid) in the pigment wastewater to reduce chemical oxygen demand. Then, the inorganic material adsorbed with bon acid was reused to prepare core-modified PR 57:1. Findings In the pigment wastewater adsorption experiment, it was found that under pH = 3, the adsorption percentage of bon acid by inorganic material can reached up to 46.00%. The pigment characterization results showed that the core-modified PR 57:1 had a core-shell structure. Under UV light irradiation for 1 h, the core-modified PR 57:1 prepared with sepiolite and SiO2·nH2O showed total color difference ΔE value of 1.43 and 2.05, respectively, which was lower than that of unmodified PR 57:1 (ΔE = 2.89). In addition, the transmittance of pigment water suspension test results showed that the core-modified PR 57:1 showed better water dispersibility. Originality/value This paper attempts to develop a synergistic strategy based on the multipurpose use of inorganic materials in adsorption treatment of pigment wastewater and preparation of core-modified PR 57:1.

Application and Evaluation of the Antifungal Activities of Glandular Trichome Secretions from Air/Sun-Cured Tobacco Germplasms against Botrytis cinerea.

The secretions of the glandular trichomes of tobacco leaves and flowers contain abundant secondary metabolites of different compounds, such as cebradanes, labdanes, and saccharide esters. These secondary metabolites have shown interesting biological properties, such as antimicrobial, insecticidal, and antioxidant activity. In this study, 81 air/sun-cured tobacco germplasms were used as experimental materials. Quantitative and qualitative analyses of the glandular secretion components were conducted using ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF MS) and gas chromatography-mass spectrometry (GC-MS). The ethanol extracts of glandular trichomes from tobacco leaves and flowers were evaluated for antifungal activity against the fungus Botrytis cinerea using the mycelial growth rate method. Orthogonal Partial Least Squares (OPLS) analysis was then performed to determine the relationship between the trichome secretion components and their anti-fungal activity. The results showed significant differences among the antifungal activities of the tested ethanol extracts of tobacco glandular trichomes. The inhibition rates of the upper leaves and flower extracts against B. cinerea were significantly higher than those of the middle and lower leaves, and 59 germplasms (73.75% of the tested resources) showed antifungal rates higher than 50%, with four germplasms achieving a 95% antifungal rate at the same fresh weight concentration (10 mg/mL). The OPLS analysis revealed that the antifungal activity was primarily associated with alpha-cembratriene-diol (α-CBT-diol (Peak7)) and beta-cembratriene-diol (β-CBT-diol (Peak8)), followed by sucrose esters III (SE(III)) and cembratriene-diol oxide. These findings help identify excellent tobacco germplasms for the development and utilization of botanical pesticides against fungi and provide a theoretical reference for the multipurpose utilization of tobacco germplasms.

Operative Benefits of Residential Battery Storage for Decarbonizing Energy Systems: A German Case Study

The reduction in PV prices and interest in energy independence accelerate the adoption of residential battery storage. This storage can support various functions of an energy system undergoing decarbonization. In this work, operative benefits of storage from the system perspective, namely, generation cost reduction and congestion mitigation, are investigated. Germany is chosen as a case study due to its strong reliance on variable renewable energy. For the analysis, an economic dispatch model with a high spatial resolution is coupled with a pan-European transmission grid model. It is shown that the system’s generation costs are highest when the assets are used only to maximize PV self-consumption, and the costs are lowest when the storage also reacts to the market dynamics. This amounts to a 6% cost reduction. Both operation strategies result in an equal level of grid congestion and infrastructure loading. This is improved with a strategy that accounts for regional peak reduction as a secondary objective. The high congestion level emphasizes that grid expansion needs to keep pace with the generation and electrification expansion necessary to decarbonize other sectors. Lastly, policymakers should enable multipurpose utilization, e.g., via the introduction of market-oriented retail electricity prices with intervention options for grid operators.

Copper-Bisbenzimidazole Complexes as Biomimetic Catalysts in Organic Transformations

Abstract: Copper complexes have biological applications as well as catalytic importance in performing organic reactions. Besides finding utilities in metalloproteins, enzymes, or molecular magnetism, they have been used extensively as catalysts in the formation ofcarbon–carbon or carbon– heteroatom bonds, in addition to many other reactions. This has been achieved by the rational selection of the organic ligands with suitable coordination sites for attaining ideal geometries. The binding of one such organic ligand, bisbenzimidazole, with copper results in complexes with varied shapes, structures, and activities, enhancing their catalytic potential and hence useful applications. This review describes the catalytic applications of bisbenzimidazole based copper (II)/(I) complexes in various useful organic transformations. It also provides an in-depth knowledge of the mechanistic pathways of the transformations involving copper complexes and their multipurpose utility as catalysts, which may help in the development of improved copper catalysts for the future.

Reversible modulation of the local environment around metal centers bearing multifunctional carbenes

Abstract This Highlight Review provides examples of external-stimuli-driven reversible modulations of the spatial, electronic, and magnetic environment around metal centers that bear multifunctional N-heterocyclic carbenes (NHCs). In particular, recent reports on Lewis-base- or Lewis-acid-mediated reversible molecular transformations of Ni(0) complexes that bear N-phosphine-oxide-substituted imidazolylidenes (PoxIms) and the corresponding imidazolinylidenes (SPoxIms) are spotlighted. We also discuss the utility of using X-ray absorption spectroscopy to examine the synthesized (S)PoxIm–Ni complexes to gain spectroscopic insight into the changes in the local environment and electronic state of the metal centers induced by these reversible transformations. Overall, this review highlights the multipurpose utility of multifunctional NHCs as a key to designing and reversibly modulating the local environment of metal centers, which paves the way for accomplishing hitherto challenging molecular transformations and developing unprecedented reactivity of multimetallic compounds.

Diversity in morpho-pomological attributes and biochemical profiling of bael (Aegle marmelos (L.) Correa) genotypes of North-Western India

Bael is a medicinal cum fruit tree with multipurpose utility and propagated mostly through seeds. The present study aimed to assess and analyse the morpho-pomological and biochemical traits of eighty seedlings grown bael genotypes comparison with two commercial cultivars (NB-5 and NB-9) of bael. The significant differences were detected among the genotypes based on the measured morpho-pomological and biochemical traits. The morpho-pomological and biochemical traits of bael exhibited variation ranging from 6.17% to 133.65%. Trunk girth ranged from 29.50 to 63.40 cm and tree spread (N–S) varied 1.00–6.30 m. Fruit length ranged from 4.60 to 12.05 cm and fruit width ranged from 4.64 to 11.72 cm. Moreover, fruit weight ranged from 56.33 to 917.65 g and pulp percentage varied from 58.64 to 81.38%. Soluble Solid Content ranged from 25.90 to 36.77 0brix and ascorbic acid varied from 14.38 to 25.45 mg/100 g. Fruit length was positively correlated with fruit width, fruit weight, pulp percentage, seed length, seed diameter and number of seeds per fruit, while it was negatively correlated with fruit surface and total number of fruit per plant. Principal component analysis showed that 76.66% of the variability observed was explained by the 13 components. Ward cluster analysis using Euclidean distance classified the genotypes into two main clusters. These findings contribute to a better understanding of the diversity and relationships among the studied genotypes, aiding future breeding and selection programs for improved bael cultivation.

Enhancing the sustainable production of cost-effective ceramics with high strength using kaolin, sewage sludge and blast-furnace slag

Solid waste treatment remains an unavoidable environmental burden for modern cities. Currently, the multipurpose utilization of solid waste in construction and building materials is worth in-depth research as it furnishes high-value commercialization pathways with environmental and economic benefits for managing waste and addressing resource scarcity. This study explored the cost-effective ceramic fabrication from 40 % sewage sludge, 10 % blast-furnace slag and 50 % kaolin through in-situ sintering method, which enhanced the sustainable production of ceramic materials. The influences of firing temperature on the ceramic sintering effects, including mineral composition, micromorphology, pore structure and process performance, were systematically investigated. Results showed that the particle consolidation and sintering degrees of finished ceramics continuously increased as the firing temperature raised, presenting different surface, cross-sectional and pore structure characteristics. This was because increasing the firing temperature changed natures of the formed glass phase, such as content and viscosity. Moreover, variations in the ceramic structure would further affect its process performance. The ceramics prepared at 1250 °C were determined to have best firing effects with the densest structure and excellent performance, and the corresponding water absorption, bulk density, apparent porosity and compressive strength were 0.97 %, 2.32 g/cm3, 2 % and 70 MPa, respectively. Finally, comparisons with other researches confirmed that the developed ceramics possessed strong competitiveness in process performance. The presented study provided data support for the value-added application and sintering optimization of waste slag and sludge in ceramic production.

Stochastic Simulation of Construction Methods for Multi-purpose Utility Tunnels

The traditional method of installing underground utilities (e.g., water, sewer, gas pipes, electrical cables) by burying them under roads has been used for decades. However, the repeated excavations related to this method cause problems, such as traffic congestion and business disruption, which can significantly increase financial and social costs. Multi-purpose Utility Tunnels (MUTs) are a good alternative for buried utilities. Although the initial cost of MUTs is higher than that of the traditional method, social cost savings make them more feasible, especially in dense urban areas. Different factors, such as the specifications of utilities, the location of the MUTs, and the construction method, should be investigated to determine if MUTs can be an economical and practical alternative. The construction method is one of the most important factors to assess to have a successful MUT project and reduce its impact on the surrounding area. Simulation can be used to investigate the different construction methods of MUTs. In this paper, two Stochastic Discrete Event Simulation models depicting two MUT construction methods (i.e., microtunneling and cut-and-cover) are developed to analyze the duration and cost of the MUT projects. Also, 4D simulation models of these methods are developed for constructability assessment of these projects.

E-Scooter for Campus Mobility

In today’s world, the infrastructure of College and Industries are becoming large so if one has to travel or visit from one place to another we have to walk a long distance and sometimes it becomes very hasty and inconvenient. Sometimes after too many travelling on campus, it causes strain and pain in the body. So to travel these distances two-wheeled like Segway PT, were introduced. We developed whole newly designed product and this isReliable, Eco-friendly, a Compact vehicle for the campus. Its utilities are college campus, Airports, Industries, Recreational Parks, Sanctuaries, Museums, Palaces, Villas etc. So our project is on design and fabrication of two-wheel vehicle and also its multipurpose utility among the society. The concept of the model taken from children’s scooter bicycle. The complete body looks like a scooter bicycle in which platform is provided for standing and driving the powered scooter. This product is a battery powered and motor-driven vehicle.

Enhancement of the Growth and Quality of Soybeans Using Wasted Coir Substrates on Multi-purpose Utilization Land

Enhancement of the Growth and Quality of Soybeans Using Wasted Coir Substrates on Multi-purpose Utilization Land

Monocular Painless Vision Loss in the Emergency Department: Multipurpose Utility of Point-of-Care Ultrasound

BackgroundEye-related symptoms are a common presentation in the emergency department (ED). The cases range from simple viral conjunctivitis to trauma-related eye injuries. One pathological condition that could lead to vision loss is retinal artery occlusion (RAO). Evaluating a patient with an eye symptom requires thorough eye examination and advanced imaging in certain instances. Consultation with an ophthalmologist is also necessary for cases that require treatment recommendations and further testing. In the ED, point-of-care ultrasound (POCUS) is a commonly used diagnostic tool that can be used for ocular examination. Case ReportWe reported a case of a 60-year-old man who presented with painless partial right-eye vision loss. POCUS showed decreased flow in the right central retinal artery with an area of the pale retina seen on the image from the retinal camera, suggesting a possible branch RAO. Further examination with POCUS showed plaque formation at the carotid bifurcation, a potential cause of the patient's symptoms. Why Should an Emergency Physician Be Aware of This?Emergency physicians and other providers should be encouraged to use POCUS to diagnose eye symptoms accurately and promptly. Abnormal findings will prompt immediate specialty consult and early appropriate management. Our case and other reported cases highlight POCUS's reliability and rapid diagnostic ability.

Identification and Evaluation of Diterpenoids from Glandular Trichome Secretions of Air/Sun-Cured Tobacco Germplasm Resources

To explore the multipurpose utilization of tobacco germplasm resources, 80 representative air/sun-cured tobacco germplasms were used as experimental materials to determine the composition and content of the diterpenoids of glandular trichome secretions using ultra-performance liquid chromatography (UPLC). Correlation analysis, cluster analysis, and principal component analysis (PCA) were used to comprehensively evaluate 80 air/sun-cured tobacco germplasms. The results are as follows: (1) 11 chromatographic peaks (Peak1–Peak11) were detected by UPLC, and the coefficient of variation of their contents ranged from 26.3 to 143%. (2) Correlation analysis showed that alpha-cembratriene-diol (α-CBT-diol) and beta-cembratriene-diol (β-CBT-diol) had the highest correlation coefficients (0.97), showing a significantly negative correlation with cis-abienol and a significantly positive correlation with the other diterpenoids. (3) PCA showed that the cumulative contribution rate of the three principal components was 75.70%, and the diterpenoid-rich germplasms were ranked in the following order: X40 (Maiduo) > X48 (Jianpingpiaoba) > X58 (Mianzhu) > X54 (Shifangpipaliu) > X63 (Xuejia5) > X41 (Tangpeng) > X29 (OLOR) > X73 (Criollo) > X44 (Tiebanqing) > X70 (Nicaragua Changxin). The diterpenoid content of X36 was the lowest. These results provide excellent germplasms for plant-based pesticides and fragrance compounds and genetic studies of the diterpenoid metabolism.

Hydrodynamic performance of a self-protected hybrid offshore wind-wave energy system

The load-bearing capacity is a pivotal consideration in the design of offshore renewable energy structures. This paper aims to address the technical challenges associated with the additional wave loads caused by the integrated devices in a hybrid system for the multi-purpose utilization of coastal renewable energy. A self-protected hybrid wind-wave energy system is proposed, in which an oscillating water column (OWC) device is attached at a monopile foundation of an offshore wind turbine. In the meantime, the OWC is connected by a submerged horizontal perforated plate at its exterior shell, which is expected to minimize the wave loads on the system. The hydrodynamic performance of the system is investigated. A novel approach is developed to model the wave interaction with the hybrid system. This efficient approach removes the necessity of decomposing the wave-scattering field into diffraction and pressure-dependent radiation components. Detailed numerical computation is then conducted for both regular and irregular sea states. Various hydrodynamic properties related to the system, such as wave energy harvesting, wave force/moment, and free-surface elevation, have been evaluated. Numerical results manifest the feasibility of imposing a negligible effect on the high wave energy harvesting while reducing the high wave loads by manipulating the submerged horizontal perforated plate. The impact of the perforated plate on the dominant wave energy harvesting in the long-period region is found to be trivial. In contrast, it can affect the high bending moment in the short-period region, which causes a reduction of greater than 15%.

Transcriptomics integrated with metabolomics to characterize key pigment compounds and genes related to anthocyanin biosynthesis in Zanthoxylum bungeanum peel.

Zanthoxylum bungeanum is an important condiment with high economic value and its peel color is one of the main quality indexes. However, the key pigment compounds and related genes are still unclear affecting the quality control of the plants. In this study, the contents of four types of pigments were measured in Z. bungeanum and flavonoids were identified as the most important pigments. Based on the targeted flavonoid metabolomics of Z. bungeanum peels, 14 key pigment compounds were screened out from 152 flavonoids, among which cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside were the most critical compounds for peel color. They were further verified to be present in nine varieties of Z. bungeanum by HPLC fingerprints. The 14 compounds were all associated with flavonoid and anthocyanin biosynthesis pathways and the 39 differentially expressed genes related to these pathways were annotated and screened based on transcriptomics. The genes ZbDFR, ZbANS, and ZbUFGT were identified as three key genes for anthocyanin synthesis in Z. bungeanum peels. Further qRT-PCR results confirmed the reliability of transcriptomics and the accuracy of gene screening. Subsequent protein induced expression demonstrated that ZbANS and ZbUFGT were expressed after 12 h induced by IPTG while ZbDFR was expressed after 15 h. Further transient and stable transformation analysis confirmed that both anthocyanin content and the expression of ZbDFR were significantly increased in overexpression Z. bungeanum leaves and Nicotiana benthamiana. The functional effect of stable transformation of ZbDFR was more significant than that of transient transformation with a 7.67-fold/1.49-fold difference in total anthocyanin content and a 42.37-fold/12.32-fold difference in the expression of ZbDFR. This study provides new insights into the chemical composition and the molecular mechanisms of Z. bungeanum peel color and lays an effective foundation for the color quality control, multi-purpose utilization of Z. bungeanum and the creation of new germplasm.

Multi-objective optimization for selecting potential locations of multi-purpose utility tunnels considering agency and social lifecycle costs

Multi-purpose utility tunnels (MUTs) and the synchronized method of underground utility intervention are two methods that have the potential to reduce the maintenance intervention lifecycle cost (LCC) of underground utilities. However, when considering the total LCC of both methods, there is a tradeoff between agency and social costs. Currently, limited research quantifies the socioeconomic impact of both methods at a macro level, especially the long-term benefits of MUTs. By quantifying the socioeconomic impacts of implementing both MUTs and the synchronized utility interventions at the network level, the LCCs of both alternatives can be optimized and compared. The main objective of this research is to optimize the agency and social LCCs and network deterioration using multi-objective optimization for identifying the potential locations of MUTs. This selection involves modeling the agency and social cost savings and the network deterioration resulting from the implementation at the network and segment levels. Comparing the optimization results in identifying the street segments in a network where the LCC of implementing MUTs is less than the LCC of the synchronized utility intervention. While doing so, a multi-year plan for MUT location selection that optimizes the three objective functions and offers lifecycle savings is achieved. The results show that out of the 312 potential street segments selected for MUT implementation, 51 offered both agency and social LCC savings. This research can aid utility owners in understanding the socioeconomic effects of both methods of utility interventions as well as identifying streets where the implementation MUTs will have significant economic advantages.

Comparative genome and metabolome analyses uncover the evolution and flavonoid biosynthesis between Apocynum venetum and Apocynum hendersonii

Apocynum species have great application prospects in textile and phytoremediation of saline soil, are rich in flavonoids, and possess medicinal significance. Here, we report the draft genomes of Apocynum venetum and Apocynum hendersonii, and elucidate their evolutionary relationship. The high synteny and collinearity between the two suggested that they have experienced the same WGD event. Comparative analysis revealed that flavone 3-hydroxylase (ApF3H) and differentially evolved flavonoid 3-O-glucosyl transferase (ApUFGT) genes are critical for determining natural variation in flavonoid biosynthesis between the species. Overexpression of ApF3H-1 enhanced the total flavonoid content and promoted the antioxidant capacity of transformed plants compared to the wild-type. ApUFGT5 and 6 explained the diversification of flavonoids or their derivatives. These data provide biochemical insight and knowledge on the genetic regulation of flavonoid biosynthesis, supporting the adoption of these genes in breeding programs aimed at the multipurpose utilization of the plants.