Background: Laparoscopic hysterectomy is popular today. Using a 3- to 4-port for laparoscopic hysterectomy. But laparoscopic hysterectomy can be done by using a single-modified glove port. It was found to have good performance and low price. Objective: To compare operative time and other surgical outcomes between single-glove port laparoscopic hysterectomy (SGPLH) and conventional multiport laparoscopic hysterectomy (MPLH). Methods: A retrospective study, data were extracted from medical records of patients diagnosed with benign gynecologic diseases and underwent laparoscopic hysterectomy at Prapokklao Hospital, Chanthaburi, Thailand from February 2016 to April 2021. Results: A total of 125 patients were divided into 2 groups 65 (52%) in the SGPLH group and 60 (48%) in the MPLH group. The operative time of SGPLH was significantly shorter than conventional MPLH (mean [SD], 96 [31.35] vs 161.4 [57.37] minutes; P < .001). There was significant difference between 2 groups in terms of previous abdominal surgery (P = .01). In multivariate regression analysis, independent factors correlated with operative time were type of laparoscopic operation (P < .001), additional procedure of bilateral salpingo-oophorectomy (P = .02), unilateral salpingo-oophorectomy (P = .01), and complication with bladder injury (P = .03). Conclusions: The operative time of SGPLH is shorter than the conventional MPLH. SGPLH is also feasible and economical for patients with symptomatic benign gynecologic diseases.