Multiple Rings Research Articles

Role of Orphan ParA Proteins in Replication and Cell Division in Rhodococcus erythropolis PR4.

Bacteria have a very well-regulated mechanism for chromosome segregation and cell division. This process requires a large number of complex proteins to participate and mediate their functionality. Among these complex proteins, ParA and ParB play a vital role for the faithful segregation of chromosome. In Rhodococcus erythropolis PR4, besides the essential parAB operon, there are three orphan copies of parA genes. Here, we report that the orphan ParA2 and ParA3 have distinct roles in the cell cycle. The disruption of the orphan parA2 or parA3 gene resulted in elongated cells. Multiple septal rings and mislocalised septa were observed in ΔparA3 and ΔparA2 mutants, respectively. The subcellular localization of ParA2 revealed a distinct ring- and ribbon-like structure. On the other hand, orphan ParA3 was localized slightly away from the poles. The orphan ParA proteins were found to interact with ParB, the strongest interaction was observed with ParA2. Further, asynchronous replication initiation was observed in ΔparA3 mutants suggesting its role in replication. This is the first report demonstrating the distinct roles of orphan parA genes from Rhodococcus.

Retrosynthetic Pathways of Decahydrofluorene-Alkaloid Microascones B

Microascones B is a compound that can be a candidate for antibacterial material and is discovered and obtained from marine-derived fungus with complex 12- or 13- membered ring structures. Since no one has provided the forward synthesis and retrosynthesis pathway, we have introduced a possible retro-synthesis pathway in this work. The retrosynthesis of this complicated compound can be done in several steps into starting materials. Some of the core retrosynthesis strategies contain the analysis of di-oxygenation patterns to break bonds retro-synthetically by applying aldol reactions and Grignard as well as anion addition. Moreover, the Diels-Alder reaction is also one of the core reactions to break those multiple rings in this retrosynthesis pathway

Hybrid Type I and II Polyketide Synthases Yield Distinct Aromatic Polyketides.

Bacterial aromatic polyketides are compounds with multiple aromatic rings synthesized by bacterial type II polyketide synthases (PKSs), some of which have been developed into clinical drugs. Compounds containing aromatic polyketides synthesized by hybrid type I and type II PKSs are extremely rare. Here, we report the discovery of a gene cluster encoding both modular type I and type II PKSs as well as KAS III through extensive bioinformatics analysis, leading to the characterization of the hybrid polyketide, spirocycline A. The structure of spirocycline A is rare among all aromatic polyketides, featuring a unique starter unit and four spirocycles and forming a dimer. Biosynthetic studies indicate that the starter unit of this molecule is synthesized by type I PKS in collaboration with two trans-acting ketoreductase (KR) and enoylreductase (ER). It is then transferred by KAS III to the type II PKS system, which synthesizes the tricyclic aromatic polyketide backbone. The subsequent formation of the spirocycle and dimerization are carried out by four redox enzymes encoded in the gene cluster. Overall, the discovery of spirocycline A provides a new approach for identifying novel aromatic polyketides and offers potential enzymatic tools for the bioengineering of these hybrid polyketides.

High‐Yielding Nanobelt Formation by Chirality‐Assisted Synthesis

Shape‐persistent Conjugated nanobelts are fascinating synthetic targets. However, in most cases these are made in low overall yields by applying strategies of macrocyclization followed by (multiple) ring fusion reactions for nanobelt formation. Here, we describe the high yielding synthesis of enantiopure chiral nanobelts in 84% yield by applying chirality‐assisted synthesis (CAS). The chiral nanobelts were investigated by UV/vis and CD spectroscopy. By DFT calculations the aromaticity of these structures is discussed.

High-Yielding Nanobelt Formation by Chirality-Assisted Synthesis.

Shape-persistent Conjugated nanobelts are fascinating synthetic targets. However, in most cases these are made in low overall yields by applying strategies of macrocyclization followed by (multiple) ring fusion reactions for nanobelt formation. Here, we describe the high yielding synthesis of enantiopure chiral nanobelts in 84% yield by applying chirality-assisted synthesis (CAS). The chiral nanobelts were investigated by UV/vis and CD spectroscopy. By DFT calculations the aromaticity of these structures is discussed.

PAMAM-Based Polymeric Immunogenic Cell Death Inducer To Potentiate Cancer Immunotherapy.

Immunogenic cell death (ICD) has been widely employed to potentiate cancer immunotherapy due to its capability to activate the anticancer immune response. Although various ICD inducers have been described, the development of synthetic materials with intrinsic ICD-inducing competency has rarely been reported. Herein, we identify a derivative of the fourth generation polyamidoamine (PAMAM) modified with multiple seven-membered heterocyclic rings, G4P-C7A, as a robust ICD inducer. G4P-C7A evokes characteristic release of damage-associated molecular patterns in tumor cells and induces efficient dendritic cell maturation. Mechanistic studies suggest that G4P-C7A can selectively accumulate in the endoplasmic reticulum and mitochondria to generate reactive oxygen species. G4P-C7A-treated tumor cells can work as potent vaccines to protect against secondary tumor implantation. Either local or systemic injection of G4P-C7A alone can effectively inhibit tumor growth by eliciting robust antitumor immune response. The combination of G4P-C7A with immunotherapeutic antibodies such as anti-PD1 (aPD-1) and anti-CD47 (aCD47) further potentiates the antitumor effect in either CT26 or 4T1 tumor model. This study offers a simple but effective strategy to induce ICD to boost cancer immunotherapy.

Spatially Varying Effect Mechanism of Intermodal Connection on Metro Ridership: Evidence from a Polycentric Megacity with Multilevel Ring Roads

Understanding the spatially varying effect mechanism of intermodal connection on metro ridership helps policymakers develop differentiated interventions to promote metro usage, especially for megacities with multiple city sub-centers and ring roads. Using multiple datasets in Shanghai, this study combines Light Gradient Boosting Machine (LightGBM) with Shapley additive explanations (SHAP) to explore these effects with the consideration of the built environment and metro network topology. Results show that the collective impacts of intermodal connection are positive, not only within the main city but also alongside the main commuting corridors, while negative effects occur in the peripheral area. Specifically, bike sharing trips increase metro ridership within the inner ring of the city, while bus services lower metro usage at stations alongside the elevated ring roads. Parking facilities enable metro usage at city sub-centers, and the small pedestrian catchment area increases metro riders alongside the main commuting corridors. Empirical findings help policymakers understand the effect mechanism of intermodal connection for stations in different regions and prioritize customized planning strategies.

Classification of multiplication modules over multiplication rings with finitely many minimal primes

Abstract A classification of multiplication modules over multiplication rings with finitely many minimal primes is obtained. A characterization of multiplication rings with finitely many minimal primes is given via faithful, Noetherian, distributive modules. It is proven that for a multiplication ring with finitely many minimal primes every faithful, Noetherian, distributive module is a faithful multiplication module, and vice versa.

Deep-Saddle-Shaped Nanographene Induced by Four Heptagons: Efficient Synthesis and Properties.

The construction of multiple heptagonal rings in nanographene is the key step for obtaining exotic carbon nanostructures with a negative curvature and intriguing properties. Herein, a novel saddle-shaped nanographene (1) with four embedded heptagons is synthesized via a highly efficient one-shot Scholl reaction from a predesigned oligophenylene precursor. Notably, a quadruple [6]helicene intermediate was also obtained and isolated by controlling the Scholl reaction conditions. Interestingly, the single crystal structures of 1 display a saddle geometry induced by the four embedded heptagons, resulting in a deep curvature with a width of 16.5 Å and a depth of 8.0 Å. Theoretical calculations at the molecular level suggest a weak antiaromatic character of the heptagons in 1. Remarkably, compound 1 exhibits dual fluorescence from S1 and S2. The deep-saddle-shaped geometry in 1 defines host-guest interactions with fullerenes, which were explored in titration experiments and by theoretical methods. The resulting 1@C60 are stable and are subject to an electron transfer from photoexcited 1 to C60. Our current study underscores the influence of heptagon rings on the photophysical, self-assembly, and electron-donating properties of NGs.

Unidirectional and hierarchical on-chip interconnected architecture for large-scale hardware spiking neural networks

Spiking Neural Networks (SNNs) exhibit the strong capability to address spatiotemporal dynamic problems. Recent research has explored the hardware SNN systems to solve the spatiotemporal problems in real-time. The Network-on-Chip (NoC) is an effective scheme for building large-scale hardware SNNs. However, for the existing NoC-based hardware SNNs, large area overhead and hardware power are consumed by their interconnections, because of complex topologies and router structures. Therefore, in this work a novel Unidirectional and Hierarchical on-Chip Interconnected Architecture (UHCIA) is proposed to address this problem. The proposed UHCIA mainly combines the novel hybrid topology of unidirectional multiple loops and rings, and uses a deflection router technique. Experimental results show that compared to other works, the UHCIA achieves ∼23.6X of area reduction and ∼6.4X of power reduction, with high system throughput and biological real-time computations.

CHALLENGE IN TREATING OPTIC NEURITIS IN PATIENT WITH CEREBRAL TOXOPLASMOSIS RELATED TO IMMUNOCOMPROMISED CONDITION

Introduction: This study aimed to discuss the prognosis of optic neuritis in a patient with cerebral toxoplasmosis related to an immunocompromised condition. Case Report: A 28-year-old female complained of blurred vision and double vision 2 weeks before admission, accompanied by headache, low extremities weakness, dysphonia, and dysphagia. Visual acuity of both eyes was 6/30 and worsened to 6/60 in a couple of days. Anterior segment examination revealed anisocoria pupil, and RAPD was positive in the left eye. Funduscopic examination showed a blurry margin and hyperemia of the optic nerve head. There was impaired eye movement, indicating oculomotor and abducens nerve palsies. High titer of IgG Antibody Toxoplasma (264) and very low titer of CD4 (<50) with non-reactive HIV rapid test were found in laboratory findings. Multiple ring enhancement was shown in MRI finding. Unfortunately, patient passed away during hospitalization due to respiratory failure. Discussion: Toxoplasmosis-associated optic neuritis is rare and usually becomes potentially serious. Although parasite detection by microscopy and bioassay is considered the gold standard for the diagnosis of toxoplasmosis, clinical diagnosis relies more on serological examination methods. The high proportion of deaths from this disease is mostly caused by late diagnosis of the infection. Conclusion: Optic neuritis that accompanied by systemic disorders needs special attention and comprehensive treatment among multidisciplinary divisions, especially in the immunocompromised condition. Early detection and primary prevention are important to improve prognosis and survival rate.

Identification and Functional Analysis of Novel Long Intergenic RNA in Chicken Macrophages Infected with Avian Pathogenic Escherichia coli.

Avian pathogenic E. coli (APEC), a widespread bacterium, results in serious economic losses to the poultry industry annually, and it poses a threat to human health due to the contaminated retail poultry meat and eggs. Recently, it has been demonstrated that long non-coding RNAs played important roles in regulating gene expression and the animal immune response. This study aimed to systematically explore the function of the novel long intergenic non-coding transcript, lincRNA-73240, upon APEC infection. A bioinformatics analysis indicated that lincRNA-73240 had no coding ability and a relative stable secondary structure with multiple hairpin rings. Moreover, the RT-qPCR results showed that lincRNA-73240 was highly expressed in lungs, heart, liver, spleen, cecum tonsils, thymus, ileum, bursa of Fabricius, harderian gland, and muscles in comparison to the cerebrum. Additionally, overexpression of lincRNA-73240 can promote the expression levels of inflammation, apoptosis, autophagy, and oxidative stress-related genes, as well as the production of reactive oxygen species (ROS), malondialdehyde (MDA), and nitric oxide (NO) upon APEC infection, which lead to cellular injury and apoptosis. These findings collectively establish a foundation for the study of the biological function of chicken lincRNA-73240 and provide a theoretical basis for further research on the molecular mechanisms of the chicken immune response.

Multiple stimuli-responsive low-molecular-weight organogel with room temperature phosphorescence for dynamic anti-counterfeiting

Pure organic luminogens with stimuli-responsive room temperature phosphorescence (RTP) have drawn much attention due to their promising applications in dynamic anti-counterfeiting. In this work, we designed a simple organic molecule with rigid chemical structure (m-FBCM), and all-atom molecular dynamics simulation indicated that it was a potential organogelator. Moreover, the molecule comprises multiple carbonyl units and aromatic rings, which could promote the intersystem crossing process. Our experiments revealed that m-FBCM could form stable gel in DMSO, promoted by balanced intermolecular π-π interactions. Green afterglow was observed at room temperature in the gel state with lifetime 41.6 ms, ascribed to its RTP. The afterglow became invisible at 45 °C and could recover upon cooling to room temperature. Moreover, the N-acylation reaction between m-FBCM xerogel and vapor of volatile primary amine could occur in a few minutes without additional catalyst, providing an orange afterglow. The thermal-responsive and chemical-reponsive properties impart the RTP gel promising application in dynamic anti-counterfeiting.

A Technique of Multiple Corneal Allogeneic Ring Segments Prepared From a Single Corneal Graft: A Case Series.

To describe a novel technique for preparing multiple corneal allogeneic ring segments (CAIRS) from a single corneal graft using femtosecond laser technology. This is a case series of 10 eyes from 10 patients with keratoconus who underwent FS-assisted CAIRS implantation using corneas from 4 donors at the Hospital Foundation Adolphe de Rothschild-Noémie de Rothschild institute. A preoperative and postoperative examination was performed at 1 day, 1 week, and 1 month. Anterior segment OCT and corneal tomography with aberrometric and pachymetric analyses were performed at each visit. Visual, refractive, and topographic parameters were extracted. The thickness and width of implanted CAIRS were analyzed. Patients were classified according to keratoconus severity: group A (maximal keratometry Kmax <75D) and group B (Kmax >75D). At 1 month postoperatively, both groups A and B showed a significant decrease in mean keratometry by 4.78 ± 1.57D and 12.87 ± 4.62D, respectively. Total and higher order aberrations decreased by 5.66 ± 4.55 and 0.65 ± 1.54 in group A and by 9.45 ± 9.15 and 0.49 ± 1.39 in group B, respectively. The corrected distance visual acuity improved by 4.8 ± 1.7 lines in group A. Visual improvement was not significant in group B. One eye in group B exhibited acute rejection and required explantation. FS-assisted multiple CAIRS implantation using a single corneal graft maximizes the utilization of viable corneal tissue. CAIRS implantation is an effective and biocompatible therapeutic alternative, particularly in cases of moderate to advanced keratoconus with Kmax <75D.

Switchable and Chemoselective Arene Hydrogenation for Efficient Late Stage Applications.

The incorporation of three-dimensional structures into drug molecules has demonstrated significant improvements in clinical success. Late-stage saturation of drug molecules provides a direct pathway for this transformation. However, achieving selective and controllable reduction of aromatic rings remains challenging, particularly when multiple aromatic rings coexist. Herein, we present the switchable and chemoselective hydrogenation of benzene and pyridine rings. The utility of the protocol has been comprehensively investigated in diversified substrates with the assistance of a fragment-screening technique. This approach provides convenient access to a diverse array of cyclohexane and piperidine compounds, prevalent in various bioactive molecules and drugs. Furthermore, it discloses promising avenues for applications in the late-stage switchable saturation of drugs, facilitating an increase in the fraction of sp3-carbons which holds the potential to enhance the medicinal properties of drugs.

Radio Images inside Highly Magnetized Jet Funnels Based on Semianalytic GRMHD Models

By performing general-relativistic radiative transfer calculations, we show the radio images of relativistic jets including highly magnetized regions inside jet funnels, based on steady, axisymmetric, and semianalytic general-relativistic magnetohydrodynamics models. It is found that multiple ring images appear at the photon frequency of 230 GHz for nearly pole-on observers, because of the strong light-bending effect on photons generated at the separation surface, which is the boundary between the inflow and outflow flows in the jet funnel. A bright teardrop-shaped component, which extends from the bright rings of the separation surface, also appears in the counterjet region. The diameter of the brightest outermost ring originated from the counterjet is ∼60 μas, which is consistent with the ringlike images of M87 at 86 GHz observed with GMVA, the Atacama Large Millimeter/submillimeter Array, and the Greenland Telescope, whose ring diameter is ∼64−8+4μas . The thinner and smaller-diameter rings are exhibited when the black hole spin magnitude is higher. These morphological features are expected to appear without being prominently affected by the detailed magnetohydrodynamic plasma parameters of our general-relativistic ideal magnetohydrodynamic (GRMHD) jet model, since the location of the separation surface is mainly regulated by the black hole spin. Our GRMHD model and the emission features of the images in the horizon-scale, highly magnetized jet funnel may be tested by future observations, e.g., the next-generation Event Horizon Telescope and the Black Hole Explorer.

Investigation of an Active Focusing Planar Piezoelectric Ultrasonic Transducer.

Ultrasonic focusing transducers have broad prospects in advanced ultrasonic non-destructive testing fields. However, conventional focusing methods that use acoustic concave lenses can disrupt the acoustic impedance matching condition, thereby adversely affecting the sensitivity of the transducers. In this paper, an active focusing planar ultrasonic transducer is designed and presented to achieve a focusing effect with a higher sensitivity. An electrode pattern consisting of multiple concentric rings is designed, which is inspired by the structure of Fresnel Zone Plates (FZP). The structural parameters are optimized using finite element simulation methods. A prototype of the transducer is manufactured with electrode patterns made of conductive silver paste using silk screen-printing technology. Conventional focusing transducers using an acoustic lens and an FZP baffle are also manufactured, and their focusing performances are comparatively tested. The experimental results show that our novel transducer has a focal length of 16 mm and a center frequency of 1.16 MHz, and that the sensitivity is improved by 23.3% compared with the conventional focusing transducers. This research provides a new approach for the design of focusing transducers.

Design and fabrication of a porous prism film for display backlight applications

This study demonstrates a fabrication method of a porous brightness enhancement film (pBEF) that offers brightness enhancement, light diffusion, color shift reduction, and improved thermal stability. During the ultraviolet imprinting and solvent evaporation processes, the nano/submicron-sized air pores are generated within the polymer prism structure, and micropatterns spontaneously form on the prism surface. The inner pores ranging from 30 to 450 nm can effectively scatter light to mitigate color shift, which is caused by multiple internal reflections within the prism structure. The micropatterns have multiple rings formed one around another with 5–15-µm diameter on the prism surface improve visual quality. Moreover, the obtained functions are achieved in a single film solution, obviating the need for using multiple materials, and the fabrication process is relatively simple and fast as it is conducted under ambient conditions. When the pBEF is integrated into a liquid-crystal display backlight, it provides the brightness enhancement performance and comparable viewing angle distribution of a regular BEF combined with an additional diffuser (two films) and increases brightness by ∼8% compared to a bead prism (particle-based BEF). Additionally, it reduces the redshift (Δxy) from 0.1605 to 0.1415. Furthermore, the pBEF exhibits a lower coefficient of thermal expansion than the regular BEF.

Multicriteria GIS-based assessment of biomass energy and hydropower potentials in Nigeria

The deployment of hybrid renewable energy systems remains the quickest way to salvage the dwindling grid supply in Nigeria and improve energy access. The assessment of biomass and hydro potentials across the geopolitical zones of the country to determine the optimal locations for a Biogas fired thermal plant for energy generation is thoroughly addressed in this paper. Multiple Geographical Information System (GIS) approaches were used to assess the energy potentials of crop and forest residues for biomass, as well as waterbodies, and waterlines for a hydrothermal power plant. The study applies the inverse distance weighting and weighted overlay multicriteria decision analysis. Forest areas, waterbodies, distance from water sources and road accessibility were considered for locating the biomass and hydrothermal facilities within multiple ring buffer distances of 200 m–2000 m from the combined biomass and hydropower resources. The result shows four states with potentials to host biomass-hydrothermal power plants in Nigeria; Niger, Kaduna, Bauchi and Taraba states with biomass potentials of 831.9 tonnes/yr, 1173.27 tonnes/yr, 322 tonnes/yr and 739.48 tonnes/yr, respectively and inland waterways. It further shows that these plants can be optimally located within 2000 m from the source of the biomass and hydro resources. This paper recommends the utilization of these results to carry out feasibility studies and make informed decisions on the exploration of these resources for power generation. It also advocates for a control in the commercialization of forest residues to sustain the production from these hybrid plants and supports the Nigerian bio-energy policy that proposes to effectively utilize the Nigeria's non-fuelwood as substitute to felling of trees.

Detection of milk adulteration using coffee ring effect and convolutional neural network

A low-cost and effective method is reported to identify water and synthetic milk adulteration of cow’s milk using coffee ring patterns. The cow’s milk samples were diluted with tap water (TW), distilled water (DW) and mineral water (MW) and drop cast onto glass slides to observe coffee ring patterns. The area of the ring, total particle area and average particle diameter were extracted from these patterns. For each ring, the ratio of total particle area versus total ring area was calculated. The area ratio, regardless of water adulterants, follows an exponential model with respect to average particle diameter. Unlike TW, the ratio for DW and MW adulterated milk are clustered and classified together with respect to the particle diameter. These results were independent of dilution level and are used for adulterant classification. The ring of milk adulterated using synthetic milk gave multiple concentric rings, flower-like structures, and oil globules throughout the dilution level. An Alexnet model was used to classify water and synthetic milk adulterants in authentic milk. The trained model could achieve 96.7% and 95.8% accuracy for binary and tertiary classification respectively. These results enable us to distinguish synthetic milk from pure milk and segregate DW and MW with respect to TW adulterated milk.