Multiple Lines Of Evidence Approach Research Articles

Occurrence and trends of historical tropical cyclone rainfall on near-coastal regions of Australia

Extreme rainfall driven by tropical cyclones (TCs) has profound effects on Australian coastlines at both local and regional scales. Here, we develop methods for comparing TC-driven widespread and localised rainfall on three broad coastal regions of tropical Australia (west, north and east). Trends, average recurrence intervals (ARIs) and the fractional contribution of TC rainfall are explored in three historical datasets: Australian Gridded Climate Data (AGCD), ECMWF Reanalysis (ver. 5, ERA5) and the Bureau of Meteorology Atmospheric high resolution Regional Reanalysis for Australia (ver. 1, BARRA1). Results for trends and ARIs between the different datasets are generally inconsistent and also differ between regions, partially owing to the short-term temporal records of some of the data as well as inconsistencies in extreme values between datasets. By contrast, there is a general agreement between all datasets on the fractional contribution of TC rainfall, signalling an increase in recent years. This result is considered together with the trend towards fewer TCs occurring in this region over recent decades, indicating a trend towards increased rainfall intensity per TC on average, assuming steady landfall rates. The methods developed here can be applied easily to other data types such as regional climate model experiments, facilitating a multiple lines of evidence approach that incorporates both observational-based and model-based data. This research is intended to help provide new methods and guidance for identifying trends in TC-driven extreme rainfall, relevant for enhanced planning and adaptation to the impacts of these extreme weather systems.

A Multiple Lines of Evidence Approach to Demonstrate Effectiveness of PFAS Remediation Technologies

AbstractSignificant investments have been made to develop treatment technologies, particularly destructive approaches, for a variety of per‐ and polyfluoroalkyl substances (PFAS) that are present in groundwater, surface water, and other environmental media. A multiple lines of evidence approach were developed to assist regulators, funding agencies, and practitioners in evaluating PFAS treatment technology performance. This article describes each of three lines of evidence that a technology is effective: (1) decrease in target PFAS concentrations is observed; (2) PFAS treatment transformation products are identified and quantified; and (3) a treatment mechanism is proposed that is consistent with previous studies and supported by data. Other considerations are also described to inform conclusions about knowledge gaps and priorities for future testing. Collectively, these lines of evidence and other considerations help communicate the complexities of PFAS treatment, strengthen research plans, standardize technology evaluator reviews, and inform realistic expectations of PFAS treatment technologies.

Environmentally relevant concentrations of chemically complex shale gas wastewater led to reduced fitness of water fleas (Daphnia carinata): Multiple lines of evidence approach

Shale gas hydraulic fracturing generates flowback waters that pose a threat to aquatic organisms if released into the environment. In order to prevent adverse effects on aquatic ecosystems, multiple lines of evidence are needed to guide better decisions and management actions. This study employed a multi-disciplinary approach, combining direct toxicity assessment (DTA) on the water flea Daphnia carinata and LC-MS metabolomics analysis to determine the impact of a major ion salinity control (SC) and a cumulative flowback shale gas wastewater (SGW) from a well in the Beetaloo Sub-basin, Northern Territory, Australia. The exposures included a culture water control, simply further referred to as ‘control’, SC at 1% and 2% (v/v) and SGW at 0.125, 0.25, 0.5, 1% and 2% (v/v). The results showed that reproduction was significantly increased at SGW 0.5%, and significantly decreased when exposed to SC 2%. SGW 2% was found to be acutely toxic for the D. carinata (< 48-h). Second generation (F1) of D. carinata exposed to 0.125–1% SGW generally saw reduced activity in four oxidative biomarkers: glutathione S-transferase, lipid peroxidation, reactive oxygen species, and superoxide dismutase. At the metabolomics level, we observed significant changes in 103 metabolites in Daphnia exposed to both SGW and elevated salinity, in comparison to the control group. These changes indicate a range of metabolic disturbances induced by SGW and salinity, such as lipid metabolism, amino acid metabolism, nucleotide synthesis, energy production, and the biosynthesis of crucial molecules like hormones and pigments. These multiple lines of evidence approach not only highlights the complexities of SGW's impact on aquatic ecosystems but also underscores the importance of informed decision-making and management practices to safeguard the environment and its inhabitants.

Diagnosing microbiologically influenced corrosion at a crude oil pipeline facility leak site – A multiple lines of evidence approach

In crude oil transmission pipelines, some parts of the system receive minimal or no fluid flow (‘dead legs’), which can on occasion lead to under deposit corrosion wherein a mixture of sediments, hydrocarbons, water, and microorganisms collectively contribute to an integrity failure. However, the microbial contribution to this phenomenon in crude oil transmission pipelines is poorly characterized. To better understand this process, we used a multiple lines of evidence approach to examine a dead leg failure event using sludge samples collected from at andnear the leak location and from non-corroded areas on the same pipe segment. Chemical analyses revealed elevated levels of acetate, total iron, and FeCO3 at the leak locations compared to non-corroded areas. Electrochemical techniques showed increased evidence for corrosion in leak site samples compared to non-corroded samples, while corrosion coupon analysis revealed many localized pits of up to 20 μm deep in leak location samples compared to fewer pits that were up to 4 μm deep from the non-corroded sample after incubating the samples for 30 days. 16S rRNA gene analysis revealed sulfide-producers, acetogens, and methanogens in all collected samples. The lack of detection of FeS as a corrosion product, no significant differences in sulfate and sulfide concentrations across all samples, and elevated levels of acetate at the leak location suggested that acetogens and/or fermentative bacteria contributed to this pipeline leak. This study highlights the use of a multiple lines of evidence approach that can be used to garner evidence to determine the contribution of MIC to failures in dead leg pipeline segments.

The dark art of interpretation in geomorphology

The process of interpretation, and the ways in which knowledge builds upon interpretations, has profound implications in scientific and managerial terms. Despite the significance of these issues, geomorphologists typically give scant regard to such deliberations. Geomorphology is not a linear, cause-and-effect science. Inherent complexities and uncertainties prompt perceptions of the process of interpretation in geomorphology as a frustrating form of witchcraft or wizardry — a dark art. Alternatively, acknowledging such challenges recognises the fun to be had in puzzle-solving encounters that apply abductive reasoning to make sense of physical landscapes, seeking to generate knowledge with a reliable evidence base. Carefully crafted approaches to interpretation relate generalised understandings derived from analysis of remotely sensed data with field observations/measurements and local knowledge to support appropriately contextualised place-based applications. In this paper we develop a cognitive approach (Describe-Explain-Predict) to interpret landscapes. Explanation builds upon meaningful description, thereby supporting reliable predictions, in a multiple lines of evidence approach. Interpretation transforms data into knowledge to provide evidence that supports a particular argument. Examples from fluvial geomorphology demonstrate the data-interpretation-knowledge sequence used to analyse river character, behaviour and evolution. Although Big Data and machine learning applications present enormous potential to transform geomorphology into a data-rich, increasingly predictive science, we outline inherent dangers in allowing prescriptive and synthetic tools to do the thinking, as interpreting local differences is an important element of geomorphic enquiry.

Multiple lines of evidence for predator and prey responses to caribou habitat restoration

How to mitigate the impacts of anthropogenic habitat alteration and restore ecological processes has become an imperative question facing applied ecology. One high-profile example in Canada is boreal woodland caribou, which are declining across North America largely due to anthropogenic habitat alteration and associated changes to predator-prey dynamics. Habitat restoration is increasingly being implemented to recover habitat, as is mandated by federal law. But given the extent of the disturbance and the cost to conduct restoration, evaluating the effectiveness of restoration treatments is needed for effective recovery of caribou populations. We evaluated the effectiveness of silvicultural treatments to reduce predator (wolf and bear) and prey (moose and caribou) use of linear features using a multiple lines of evidence approach. All four species were less likely to be present at treated sites than untreated sites, and daily photo capture rates of moose and wolves when present at treated sites also declined; though effect sizes were typically small. Complimenting the camera-based results, individual moose, bears, and wolves monitored with GPS collars also showed a decline in the use of treated linear features, particularly those with higher intensity treatments, though the response was non-significant. The reduction in the use of treated lines did not scale-up into a significant decline in overall line-use within the treatment area. While we found more evidence than not supporting that animals reduced use of the restoration sites, our study highlights the complexity of monitoring and evaluating the success of habitat restoration. Understanding long-term responses is imperative to ensure habitat restoration is effective.

Decoupling wastewater impacts from hydrogeochemical trends in impacted groundwater resources

In the urban environment, anthropogenic activities provide numerous potential sources of contamination, which can often lead to difficulties in identifying the processes impacting groundwater quality (natural and anthropogenic). This is particularly relevant at Wastewater Treatment Plants (WWTPs) that are often subject to changes in land use and composition of contaminant sources over time and space, as well as multiple potential hydrogeochemical interactions. To help address this issue, we demonstrate how long-term time-series analysis of major ions and key contaminants of concern, which are routinely collected by WWTP operators, can be analysed using hydrogeochemical plotting tools, multivariate statistics and targeted isotopic analysis, to provide a means of better characterising key hydrogeochemical influences and anthropogenic inputs. Application of this approach to a WWTP in south-eastern Australia indicated that anthropogenic impacts were the primary driver influencing the local hydrogeochemical environment and groundwater quality. However, secondary processes, including mineral (particularly calcite) dissolution, ion exchange and possible dedolomitisation, as well as natural degradation/transformation of contaminants were also important. Long-term, time-series analysis of trends in NO3-N, NH4-N, Ca2+, SO42−, HCO3− and K+ in conjunction with the other lines of evidence, allowed for enhanced separation between individual contaminant sources, particularly when paired with a detailed site history and Conceptual Site Model (CSM). This indicated that off-site agricultural impacts post-date most site derived impacts, and to date, have not significantly added to the identified contaminant plume. The outcomes of this work have significant global application in the identification, assessment, and control of environmental and health risks at complex sites and show how significant value (rarely obtained) can be derived from the analysis of routine monitoring datasets, particularly when analysed using a multiple lines of evidence approach.

A weight-of-evidence approach for identifying potential sources of untreated sewage inputs into a complex urbanized catchment.

The Hawkesbury-Nepean River (HNR) is the largest catchment in the Sydney region and is undergoing unprecedented population growth. The HNR system receives a mix of anthropogenic inputs such as treated sewage, stormwater and agricultural runoff. Combined, these can diminish the ecological system health and pose potential concerns to human health. Of particular concern are inputs of untreated sewage, that can occur due to a range of different reasons including illegal point source discharges, failure of the sewerage network, and overloading of wastewater treatment plants during storm events. Here, we present findings of an intensive assessment across the HNR catchment where we used a weight-of-evidence (WOE) approach to identify untreated sewage contamination in surface waters against the background of treated effluent and diffuse inputs during post high flow conditions. Total nitrogen and phosphorus concentrations were used to assess treated effluent and diffuse inputs, and microbial analysis, including both culture-based traditional methods for E.coli and enterococci and qPCR analysis of Bacteroides and Lachnospiraceae, were used to assess raw sewage contamination. Despite a background of diffuse inputs from recent high flow events and the influence of treated wastewater, we found no gradient of faecal contamination along the HNR system or its tributaries. We observed two sites with evidence of untreated sewage contamination, where the human markers Bacteroides and Lachnospiraceae qPCR copy numbers were high. The biological and chemical approaches suggested these latter two hotspots originate from an industrial runoff source and possibly from a dry weather sewage leak. Our findings demonstrate the potential of a WOE approach in the assessment of human faecal signal in an urban river that can also pinpoint small sources of contamination as a strategy that can reshape the way monitoring is performed and the chemical end-points chosen to provide pertinent information on the potential risks to aquatic system health.

Industry-collected target strength of high seas orange roughy in the Indian Ocean

Abstract Visually verified in situ target strengths (TS) are the state of the science for determining the conversion from acoustic echo-integration surveys to biomass. Here, we show how these measurements can be made by high seas fisheries during normal operations using a net-attached acoustic optical system (AOS) without specialized personnel on board. In situ TS were collected from ∼45 cm standard length (SL) orange roughy (Hoplostethus atlanticus) in the southern Indian Ocean at 38 and 120 kHz. We use a multiple lines of evidence approach to demonstrate that the previous TS–SL equation developed for ∼10 cm smaller fish in Australia and New Zealand is not suitable for the larger orange roughy and instead propose new TS–SL equations. Our findings show that biomass estimates at 38 kHz will be reduced by ∼58% when using this new TS–SL compared to the existing TS–SL for smaller fish. This highlights the error of extrapolating TS–SL equations outside the measurement range. For this high sea region, the net-attached AOS represented a practical cost-effective method to obtain measurements and provide a result that could be used to inform the management of the stocks. We suggest that this method would be useful in all deep-water fisheries to monitor the TS of the fish for a range of environmental and ontogenetic conditions.

Multiple Bioassays and Targeted and Nontargeted Analyses to Characterize Potential Toxicological Effects Associated with Sediments of Masan Bay: Focusing on AhR-Mediated Potency.

An enhanced, multiple lines of evidence approach was applied to assess potential toxicological effects associated with polluted sediments. Two in vitro bioassays (H4IIE-luc and Vibrio fischeri) and three in vivo bioassays (microalgae: Isochrysis galbana and Phaeodactylum tricornutum; zebrafish embryo: Danio rerio) were applied. To identify causative chemicals in samples, targeted analyses (polycyclic aromatic hydrocarbons (PAHs), styrene oligomers (SOs), and alkylphenols) and nontargeted full-scan screening analyses (FSA; GC- and LC-QTOFMS) were performed. First, great AhR-mediated potencies were observed in midpolar and polar fractions of sediment extracts, but known and previously characterized AhR agonists, including PAHs and SOs could not fully explain the total potencies of samples. Enoxolone was identified as a novel AhR agonist in a highly potent sediment fraction by use of FSA. Enoxolone has a relative potency of 0.13 compared to benzo[a]pyrene (1.0) in the H4IIE-luc bioassay. Nonylphenols associated with membrane damage that influenced the viability of the microalgae were also observed. Finally, inhibitions of bioluminescence of V. fischeri and lethality of D. rerio embryos were strongly related to nonpolar compounds. Overall, the present work addressed assay- and end point-specific variations and sensitivities for potential toxicities of mixture samples, warranting a significant utility of the "multiple lines of evidence" approach in ecological risk assessment.

Application of a multiple lines of evidence approach to document natural attenuation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in groundwater

This study utilized innovative analyses to develop multiple lines of evidence for natural attenuation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in groundwater at the U.S. Department of Energy’s Pantex Plant. RDX, as well as the degradation product 4-nitro-2,4-diazabutanal (NDAB; produced by aerobic biodegradation or alkaline hydrolysis) were detected in a large portion of the plume, with lower concentrations of the nitroso-containing metabolites produced during anaerobic biodegradation. 16S metagenomic sequencing detected the presence of bacteria known to aerobically degrade RDX (e.g., Gordonia, Rhodococcus) and NDAB (Methylobacterium), as well as the known anoxic RDX degrader Pseudomonas fluorescens I–C. Proteomic analysis detected both the aerobic RDX degradative enzyme XplA, and the anoxic RDX degradative enzyme XenB. Groundwater enrichment cultures supplied with low concentrations of labile carbon confirmed the potential of the extant groundwater community to aerobically degrade RDX and produce NDAB. Compound-specific isotope analysis (CSIA) of RDX collected at the site showed fractionation of nitrogen isotopes with δ15N values ranging from approximately −5‰ to +9‰, providing additional evidence of RDX degradation. Taken together, these results provide evidence of in situ RDX degradation in the Pantex Plant groundwater. Furthermore, they demonstrate the benefit of multiple lines of evidence in supporting natural attenuation assessments, especially with the application of innovative isotopic and –omic technologies.

Chlorinated ethene plume evolution after source thermal remediation: Determination of degradation rates and mechanisms

The extent, mechanism(s), and rate of chlorinated ethene degradation in a large tetrachloroethene (PCE) plume were investigated in an extensive sampling campaign. Multiple lines of evidence for this degradation were explored, including compound-specific isotope analysis (CSIA), dual C-Cl isotope analysis, and quantitative real-time polymerase chain reaction (qPCR) analysis targeting the genera Dehalococcoides and Dehalogenimonas and the genes vcrA, bvcA, and cerA. A decade prior to this sampling campaign, the plume source was thermally remediated by steam injection. This released dissolved organic carbon (DOC) that stimulated microbial activity and created reduced conditions within the plume. Based on an inclusive analysis of minor and major sampling campaigns since the initial site characterization, it was estimated that reduced conditions peaked 4 years after the remediation event. At the time of this study, 11 years after the remediation event, the redox conditions in the aquifer are returning to their original state. However, the DOC released from the remediated source zone matches levels measured 3 years prior and plume conditions are still suitable for biotic reductive dechlorination. Dehalococcoides spp., Dehalogenimonas spp., and vcrA, bvcA, and cerA reductive dehalogenase genes were detected close to the source, and suggest that complete, biotic PCE degradation occurs here. Further downgradient, qPCR analysis and enriched δ13C values for cis-dichloroethene (cDCE) suggest that cDCE is biodegraded in a sulfate-reducing zone in the plume. In the most downgradient portion of the plume, lower levels of specific degraders supported by dual C-Cl analysis indicate that the biodegradation occurs in combination with abiotic degradation. Additionally, 16S rRNA gene amplicon sequencing shows that organizational taxonomic units known to contain organohalide-respiring bacteria are relatively abundant throughout the plume. Hydraulic conductivity testing was also conducted, and local degradation rates for PCE and cDCE were determined at various locations throughout the plume. PCE degradation rates from sampling campaigns after the thermal remediation event range from 0.11 to 0.35 yr−1. PCE and cDCE degradation rates from the second to the third sampling campaigns ranged from 0.08 to 0.10 yr−1 and 0.01 to 0.07 yr−1, respectively. This is consistent with cDCE as the dominant daughter product in the majority of the plume and cDCE degradation as the time-limiting step. The extensive temporal and spatial analysis allowed for tracking the evolution of the plume and the lasting impact of the source remediation and illustrates that the multiple lines of evidence approach is essential to elucidate the primary degradation mechanisms in a plume of such size and complexity.

Integrative assessment of sediment quality in acidification scenarios associated with carbon capture and storage operations

Efforts to stem global change include the application of new technological developments that aim to reduce atmospheric carbon dioxide (CO2) through the carbon capture and storage (CCS) of this greenhouse gas in stable geological structures. In this paper, we assess the potential risks related to the application of CCS technology and the acidification of aquatic ecosystems through CO2 enrichment. We use the multiple lines of evidence approach (LOEs) to characterize the effects of the acidification with contamination in aquatic sediments. We highlight and discuss the effects of acidification on the LOEs including contamination and mobility of contaminants in sediments, toxicity, macrobenthic community structure, in situ effects, and bioaccumulation–biomagnification processes. We further assess the results of acidification on the toxicity of organic contaminants such as antibiotics or illicit drugs like the freebase form of cocaine (crack). The main goal of using the LOE approach is to distinguish between adverse effects that are associated with contaminants and those related to acidification by enrichment of CO2 as a result of CCS technology. Previous assessments were not designed or conducted to incorporate an integrative point of view, nor did they employ a weight of evidence approach (WOE) in risk characterization and management of CCS operations and other situations related to acidification by enrichment of CO2 in the aquatic ecosystem. Based on the findings of this review, the WOE can identify the effects of the acidification on the different LOEs used for sediment quality: contaminant mobility, the adverse effects in organism under laboratory and field conditions, and the bioaccumulation–biomagnification of contaminants. The main strength in using the WOE is the ability of this method to discriminate between LOE responses associated with contamination by different organic and inorganic substances from those related to CO2 acidification itself. The WOE will significantly improve the risk assessment in areas affected by potential leakages of CO2 during CCS operations.

A multiple lines of evidence approach for identifying geologic heterogeneities in conceptual site models for performance assessments.

Model-based decision making is commonly used in performance assessments to assure water resource protection for both human health and the environment for hundreds of years into the future. To make decisions regarding aquifer protection against potential contamination, a conceptual site model (CSM) describing the hydrodynamic behavior needs to account for subsurface heterogeneities in sufficient detail. When site-specific data are sparse, larger-scale geologic descriptions are adopted with the consequence of losing small-scale features (at the cm scale) that can control contaminant transport. In this study, a multiple lines of evidence approach is used to construct vadose zone CSMs based on an evaluation of several types of data, including geologic logs, borehole moisture content and concentration data, geophysical spectral gamma logging data, and groundwater concentration data for a tank farm at the Hanford Site in southeastern Washington State. The resulting CSMs of the unsaturated zone represent a synthesis of what is known about flow and transport processes at the site-scale and maintain consistency with knowledge that has been accumulated at the regional scale. Through a process of extensive data analyses, a systematic approach is described to create an evidence base that supports the evaluation and development of CSMs. Numerical models are then used to evaluate the impact that smaller-scale heterogeneities have on contaminant transport through the vadose zone for a performance assessment on waste tank closure. Together, the field data and the numerical experiments suggest that although small-scale features close to source releases can have an impact on horizontal spreading, overall there is a relatively minor impact on transport for the site under study as evaluated by differences in peak fluxes and arrival times for historical leak events, and for potential releases resulting from waste tank closure. Use of alternative CSMs, developed through careful examination of available characterization and monitoring data, provides confidence that geologic heterogeneities do not impact contaminant transport behavior significantly enough to alter the assessment of risk for closure at this site.

Vapor intrusion evaluation for redevelopment of former industrial facilities

Former industrial facilities in our cities are finding new life as they are repurposed for use as nonindustrial commercial or even residential spaces. Often, these buildings are brownfield properties, complicating redevelopment because of past industrial uses that led to soil and/or water contamination at the site. In case site contaminants include volatile chemicals (VCs), concerns about air quality at the site may arise. VCs can vaporize from the subsurface and migrate through soil to outdoor air or into indoor spaces of overlying buildings, where they may accumulate.Vapour intrusion is a relevant problem especially at sites where building modernization and revitalization is to be carried out, as environmental investigations may be complicated by the structure of the built environment, and the contamination may remain undetected. Moreover, upgrading structures to meet building codes and energy conservation requirements can create tight buildings that may enhance the effects of vapor intrusion.For accurately predicting whether indoor air quality is being or will be adversely affected by subsurface contaminations a multiple lines of evidence approach should be used. Many issues can in fact introduce uncertainty in predicting indoor air concentrations related to vapor intrusion, including i) sampling and analytical methods ii) modelling of fate & transport from subsurface into building, iii) indoor/ambient background sources.This work refers about the risk management strategy at a site in Milan (Italy) where soil remediation (excavation and off-site disposal) left a residual volume of soil polluted with petroleum-derived hydrocarbons next to redeveloped buildings. Furthermore, the site was also affected by an extended plume of chlorinated solvents in groundwater from an unknown source, likely external to the site. Indoor air, outdoor air, crawl-space air and soil gas samplings were carried out to collect robust information for evaluating decisions points in the vapor intrusion process. Despite few ambient air and crawl-space measurements resulted in episodic high values, as a general trend, the average outdoor and indoor concentrations did not differ significantly from the background values, suggesting other sources than soil pollution were affecting the quality of the air at the site. Although not strictly necessary in terms of time-averaged health risk, a mitigation system of the residual soil contamination was, however, installed to prevent future uncontrolled exposure.

Assessment of potential mercury toxicity to native invertebrates in a high-gradient stream.

This study evaluated potential effects of mercury (Hg) on benthic macroinvertebrates in the South River, Virginia, USA. The study used a multiple lines of evidence approach, including spatially and temporally matched sediment chemistry, biological, and toxicological information (Sediment Quality Triad), exposure and effect analysis in bulk and interstitial sediment and interstitial water, and critical body residue analysis. Ten-day Chironomus dilutus and Hyalella azteca toxicity tests established site-specific no-effect concentrations (NOEC) at 18.9 μg/g total Hg (THg) and 102 ng/g methylmercury (MeHg). However, the benthic community at these locations was impaired, with lower mayfly and caddisfly composition. Few locations had concentrations of THg and MeHg that exceeded the NOEC in bulk or interstitial sediment. The THg concentrations in interstitial water were far below concentrations expected to reduce survival in benthic invertebrates, and only a low percentage of samples exceeded sublethal (growth) low-effect concentrations (LOEC) for THg or MeHg. The THg concentrations in invertebrate tissue did not exceed the NOEC or LOEC in the South River, and MeHg concentrations exceeded the LOEC at all locations, including those with no evidence of benthic impairment, illustrating the uncertainty associated with this line of evidence. Finally, statistical modeling that evaluated diversity of sensitive invertebrate species as a function of Hg exposure, geomorphological parameters, and physicochemical variables indicated that physicochemical and geomorphological parameters were most predictive of benthic community; where Hg was indicated, we were unable to distinguish between models with or without interstitial water Hg. Overall, the lines of evidence indicate that Hg, while clearly toxic to invertebrates at sufficiently high exposure concentrations, is not negatively impacting invertebrate communities in the South River. This study illustrates the difficulty of assessing risks to invertebrates using traditional tools of risk assessment and identifies critical gaps in knowledge that complicate the management of Hg risk. Integr Environ Assess Manag 2019;00:000-000. © 2019 SETAC.

Methods and Advances in the Forensic Analysis of Contaminated Rivers

Trace metals and metalloids are a common and persistent form of riverine (river) contamination and are derived from a wide variety of sources, including mining and milling operations, industrial activities, urban runoff, agricultural chemicals, and atmospheric pollution, among a host of others. Documentation of trace metal sources and dispersal pathways in riverine ecosystems is essential to mitigate their potentially harmful effects to human and ecosystem health and is often required from a legal (environmental forensic) perspective to assess liability for the costs of remediation. Unfortunately, documenting the sources and source contributions of trace metals in rivers has proven difficult, time-intensive, and costly. Herein, a four-component, interdisciplinary framework is proposed to efficiently identify the sources and source contributions of trace metals in alluvial sediments where multiple natural and/or anthropogenic sources exist. The components include (1) the analysis of the river’s alluvial stratigraphic architecture and geomorphic history, (2) the temporal correlation of geochemically characterized alluvial deposits to potential anthropogenic trace metal sources, (3) the analysis of the spatial variations in selected geochemical parameters, and (4) the use of geochemical and/or isotopic tracers to quantitatively estimate the contributions of trace metals from the defined natural and anthropogenic sources. The four components arenotintended to be exhaustive; the framework may require modification following multiple lines of evidence approach, in which additional methods and data are added to the investigation until there is confidence that all trace metal sources and their contributions have been effectively defined.

Sediment quality assessment in tidal salt marshes in Northern California, USA: An evaluation of multiple lines of evidence approach

Sediment quality assessment in tidal salt marshes in Northern California, USA: An evaluation of multiple lines of evidence approach

Sediment quality in a metal-contaminated tropical bay assessed with a multiple lines of evidence approach

A sediment quality assessment was performed near to the main industrial source of metal contamination in Sepetiba Bay, Brazil, which represents one of the worst cases of trace metal contamination reported for coastal areas. Acute and chronic toxicity tests, benthic fauna community analysis and metal bioavailability evaluations were applied to identify risks to the benthic community. Significant amphipod mortality was observed close to the major pollution source and lower copepod fertility was observed for all stations. Equilibrium-partitioning and biotic-ligand models to predict pore water metal toxicity, which were based on acid-volatile sulfide (AVS) and organic carbon fraction (fOC) normalization approaches, suggested that metals are not likely to be available in sediment pore water. However, Cd, Pb and Zn concentrations were mainly (>50%) weakly bound to sediments, suggesting high potential bioavailability. Linking the chemical results with ecotoxicological responses, we observed that sediment-feeding organisms presented acute and chronic toxicities that were positively correlated to the metal concentrations in the sediments. Additionally, benthic fauna composition was dominated by tolerant species, revealing a trophic structure response to environmental contamination. These results reinforce the necessity of a multiple lines of evidence approach to establish sediment quality and to support environmental management decisions that are based on observed effects and potential extrapolation scenarios into the future.

Fingerprinting of Weathered Oil Residues from the Deepwater Horizon Oil Spill: The Importance of Multiple Lines of Investigation

ABSTRACT 2017-189 The crude oil released from the Macondo Well, also known for its location in Mississippi Canyon area as the MC252 well during the Deepwater Horizon Oil Spill, entered an environment already containing a complex mixture of hydrocarbons from both natural and anthropogenic sources, many of which have closely related chemical profiles. To understand the impact of the released oil in offshore areas, a method was needed to distinguish MC252-related hydrocarbons from other sources. A multiple lines of evidence approach was developed to identify weathered MC252 oil in offshore sediments in the Gulf of Mexico. Chemical data for alkanes, PAH, petroleum biomarkers and metals were combined with spatial, temporal, and observational information to examine the fingerprints for more than 4,000 sediment samples collected over the span of five years. The unique conditions of the Gulf of Mexico (GOM), with many natural petroleum seeps and tepid seas, provided an ideal environment to support microbial degradation of petroleum. As a result of these conditions, the initial fingerprint of the MC252 was rapidly and extensively altered in the environment including depletion of petroleum biomarkers, usually assumed to be recalcitrant and often used in ratios to identify petroleum residues. Revised biomarker match criteria were defined to account for biodegradation within this fraction. Applying this methodology to the offshore sediment data from the GOM provided a comprehensive understanding of the distribution of the MC252 oil in offshore sediment and an understanding of the various transport pathways which conveyed the oil to the sediments.