This article presents identification methods of the natural frequency of structures from single or multiple measurements in the frequency domain and its application to identify the natural frequencies of the offshore truss structure. The simulated measurements of structural vibrations in the time domain are determined by analyzing the finite element model of the offshore truss structure subjected to random dynamic loads using SAP2000 software. The frequency domain measurements are obtained by the fast Fourier transform of time domain measurements using MATLAB software. The identification method from the multiple combined frequency response function measurements can estimate more natural frequencies than using a single measurement. A study of the considered frequency range was also performed showing the accuracy of the identification methods compared with the finite element model. By subdividing the considered frequency range, it is possible to improve the accuracy of the obtained natural frequencies.