Multiple Cultures Research Articles

NIMG-86. DISSEMINATEDCRYPTOCOCCUS NEOFORMANS MIMICKING CENTRAL NERVOUS SYSTEM PROGRESSION OF DIFFUSE LARGE B-CELL LYMPHOMA

Abstract A 57-year-old male with stage IV diffuse large B-cell lymphoma (DLBCL), non-germinal center subtype, underwent 6 cycles of polatuzumab, rituximab, cyclophosphamide, doxorubicin, prednisone, and pegfilgrastim (Pola-R-CHP) then presented to the hospital with progressive confusion, double vision, hearing loss, and lower extremity weakness. MRI of the brain and spinal cord with and without IV contrast revealed extensive leptomeningeal enhancement along the brain, several cranial nerves, cervical and thoracic spinal cord with enhancement of the cauda equina. Cerebrospinal fluid (CSF) analysis studies revealed 41 nucleated cells/mm3, protein to 264 mg/dL, and an initial cryptococcal antigen titer of 1:1280; serial lumbar punctures peaked at an opening pressure of 39.5 cm H2O before improvement. Flow cytometry was negative on two CSF studies. Multiple cultures grew Cryptococcus neoformans before sterilization of CSF culture on day 13. He received induction therapy for 4 weeks with amphotericin B and flucytosine. Flucytosine treatment was limited due to cytopenias on day 9 and continued induction with amphotericin B and fluconazole. Fluconazole was switched for voriconazole based on minimal inhibitory concentrations by broth dilution, however, the drug was undetectable on therapeutic drug monitoring 2 weeks later and switched back to fluconazole. Subsequent positron emission tomography revealed ongoing hypermetabolic thoracic, mediastinal, and hilar lymph nodes compared to PET scans prior to chemotherapy for which he underwent transbronchial fine needle aspiration concerning for Cryptococcus involvement. He remained on fluconazole 800mg/day and MRI 11 months after the initial presentation demonstrated ongoing but slightly improved leptomeningeal enhancement. Due to persistent symptoms despite sterile CSF cultures, he underwent a three-day course of IV methylprednisolone 1000mg with some incomplete improvement in symptoms. The opening pressure normalized and the cryptococcal antigen titer decreased to 1:20 about 11 months after initial treatment. This case highlights the risk of opportunistic infections like cryptococcal meningitis in patients undergoing chemotherapy.

Do bilinguals have an emotional advantage? An exploratory study on the interplay of language, culture and emotion

ABSTRACT Developing emotional competence during childhood is critical because it is a significant resource for building psychological well-being, social relationships and even school achievement. Language plays an important role in fostering emotional competence among monolingual children but for bilingual children, the relationship between culture, language and emotions is significantly more complex. Although bilinguals may have more emotion concepts due to their engagement in multiple languages and cultures, this potential may only materialise under specific conditions. In this study, we investigate whether ethnic minoritised bilingual children’s emotional competence (i) differs from that of majority monolingual children based on their language proficiency profile and (ii) is shaped by their exposure to the majority culture, independent of their language competencies. Using a sample of primary school children (aged 10–12; N = 924) living in Belgium, the results of our quantitative analyses suggest that being fluently bilingual is beneficial to fostering emotional competence for minoritized children. However, Belgian socialisation efforts, exposure to Dutch media and demographic proxies of the degree of cultural exposure were not significantly related to emotional competence. Our results contradict contemporary deficit views surrounding bilingualism and suggest important policy implications for educational professionals.

A Corpus-Based Evaluation of English Readers’ Aesthetic Reception of the Shandong-English Translation in Red Sorghum

In the context of world literature, target readers’ aesthetic reception of literary works has shifted from single culture to multiple cultures and has become a crucial factor in social-cultural communication. Shandong dialect, previously misidentified as vulgar expressions in literary research, has proved to have specific aesthetic values as explored in this research. This paper aims to analyse reasons for aesthetic criticism and find solutions to enhance the aesthetic acceptability of Shandong-English translation in Red Sorghum among English readers. Data were collected from different channels, including scholarly critiques, book reviews, blog posts by literary critics, and online forums. A corpus-based methodology is utilized to examine the aesthetic acceptability and reasons for aesthetic criticism by employing a re-framed aesthetic reception theory. Results showed greater frequency of aesthetic acceptance in book reviews than in online forums and blog posts by literary critics, with 53.7 per cent aesthetic acceptance versus 39.7 per cent aesthetic criticism from a total 529 sample data, excluding 6.6 per cent neutrality. Furthermore, book reviews showed a greater tendency towards aesthetic acceptance (64.3%) than online forums (51.5%) and blog posts by literary critics (33.3%). Lastly, the reasons for aesthetic criticism were mainly demonstrated in cultural disparity and defamiliarization of Shandong dialectal expression. The paper concludes by underscoring the significance of shifting from a text-oriented tendency to readers’ expectancy, initiative interaction, and social consciousness in the interaction with literary works. The study contributes reconstructed theory and practical insights for translators to balance the original cultural references and acceptability among English readers.

Clinical application of targeted next generation sequencing in detecting rifampicin and rifabutin resistance in tuberculosis patients

To evaluate the clinical value of targeted next generation sequencing (tNGS) in diagnosing rifampicin and rifabutin resistance in tuberculosis patients. In this retrospective cohort study, 119 culture-positive Mycobacterium tuberculosis (MTB) strains from tuberculosis patients in Shenzhen Third People's Hospital from 2020 to 2023 were collected, then tNGS was performed to detect mutations of rpoB gene. Fourteen different types of rpoB gene mutation were detected in 46 mutation MTB strains, including 43 resistance related mutations and 3 synonymous mutations at codon 529. Using the phenotypic drug susceptibility results of rifampicin and rifabutin as the reference standard, the sensitivities of tNGS for detecting resistance to rifampicin and rifabutin were 100%, and the specificities were 96.2% and 89.4% respectively, therefore, tNGS showed good diagnostic performance. Mutations at positions 531 and 526 of rpoB were highly associated with resistance to rifampicin and rifabutin. Moreover, the results of tNGS from the clinical specimens were consistent with those from the corresponding culture strains. tNGS analysis was performed on 83 MTB strains from 18 patients with multiple positive cultures. The results showed that 2 patients with no mutations in the initial MTB strains were subsequently detected with rpoB gene mutation and their phenotypic drug susceptibilities changed from sensitive to resistant. In summary, using tNGS to detect rpoB mutations can reduce false positive results caused by synonymous mutations, and have satisfactory performance for the diagnosis of rifampicin and rifabutin resistance. tNGS can directly detect clinical sputum samples, and also can be used to dynamically monitor the molecular resistance profiles of MTB, therefore it has extremely broad clinical application prospects.

Prevalence of Premature Ejaculation: A Narrative Review of National and Cultural Differences

The prevalence of premature ejaculation (PE) across different cultures and nationalities has long been a topic of debate, with early studies suggesting that men in such regions as the Middle East and Asia experience higher rates. However, the validity of these claims has been questioned due to methodological inconsistencies and the lack of comparative studies that apply uniform diagnostic criteria across different populations. This narrative review aims to analyze the existing literature to assess whether justifiable support for cultural or national differences regarding PE prevalance truly exists. The review focused on empirical studies spanning from 1995 to 2024 that included men from multiple nationalities or cultures. Studies on patient or specialized populations or that assessed only ejaculation latencies (EL) were excluded. Three categories of studies were reviewed: (1) studies including multiple nationalities within a single analysis, (2) studies from different countries that used an identical method to determine PE status, and (3) studies employing varied methodologies to determine PE status. The review found that differences in PE prevalence across cultures are more likely due to methodological variations than true cultural or national differences. While some trends, such as slightly higher rates in Latin American and Asian countries, were noted, no consistent patterns emerged. Studies using standardized methods, like the PEDT, generally showed prevalence rates between 5% and 15%, with no clear outliers across regions. Thus, current evidence does not support significant cultural or national differences in PE prevalence. Methodological inconsistencies, such as reliance on self-report and non-standardized diagnostic criteria, likely contribute to perceived differences. Future research should focus on using standardized methodologies and include more studies from underrepresented regions, such as Latin America and sub-Saharan Africa.

Assessing the measurement invariance of Free Will and Determinism Plus scale across four languages: a registered report.

Free will is assumed to be the core of an individual's self-concept. Belief in free will has been studied extensively and was found to be correlated with many behavioural and psychological outcomes. Although developed and validated in the West, the Free will and Determinism Plus (FAD-Plus) scale has been translated, used, and interpreted as a measurement of free will beliefs in multiple cultures. However, the cross-cultural measurement invariance of FAD-Plus has not been examined. Given the cultural differences in understanding the concept of 'free will', items of FAD-Plus may have different interpretations in different cultures, which may compromise its cross-cultural measurement invariance. To provide empirical evidence for the lack of cross-cultural measurement invariance, we collected data in China and analyzed these data together with open datasets of FAD-Plus in three other languages: Japanese, French and English. We only found partial measurement invariance between the Chinese and English datasets, as well as the Japanese and English datasets. These results provided the first assessment of cross-cultural measure invariance of FAD-Plus. We discussed the potential implications of the current study for future studies in the field.

Erysipelothrix rhusiopathiae-associated bloodstream infection in a patient with systemic lupus erythematosus: a case report and literature review

Introduction. Systemic human infections caused by Erysipelothrix rhusiopathiae have been increasingly reported especially within immunocompromised hosts and those with significant occupational exposure to livestock and aquatic animals. We report a case of E. rhusiopathiae bacteraemia in a patient with systemic lupus erythematosus (SLE) and present a literature review on clinical outcomes and microbiologic diagnosis for this organism. Case presentation. A 43-year-old female patient was reporting a 1-month history of intermittent fevers. She recently increased her immunosuppression medication for her underlying SLE on the advice of her rheumatologist. The patient sustained a finger laceration from butchering cattle meat 2 weeks after the onset of her initial symptoms, with worsening index finger swelling and increased febrile episodes. Two weeks post-injury, multiple blood cultures were drawn, and each isolated Gram-positive bacilli. Given her recurrent intermittent fevers, there was a concern for ongoing infection, and therefore, intravenous vancomycin was started with prompt referral to an outpatient parenteral antibiotic therapy clinic. The Gram-positive bacillus was confirmed as E. rhusiopathiae via matrix-assisted laser desorption/ionization-time of flight analysis. Given intrinsic resistance to vancomycin, vancomycin was switched to intravenous ceftriaxone as targeted antimicrobial therapy for 2 weeks. Reassuringly, there was no echocardiographic evidence of infective endocarditis, warranting the prolonged treatment course. Post-treatment, she remained symptom-free with the resolution of joint symptoms and fevers. Conclusion. Our report illustrates a case of E. rhusiopathiae bacteraemia from an immunodeficient host, with prompt microbiologic diagnosis and intervention with appropriate antimicrobial coverage. Literature reflects the rarity of this infection, predilections to specific susceptible hosts and the importance of raising awareness of zoonotic infections.

Efficient Simulation of Viral Transduction and Propagation for Biomanufacturing.

The design of biomanufacturing platforms based on viral transduction and/or propagation poses significant challenges at the intersection between synthetic biology and process engineering. This paper introduces vitraPro, a software toolkit composed of a multiscale model and an efficient numeric technique that can be leveraged for determining genetic and process designs that optimize transduction-based biomanufacturing platforms and viral amplification processes. Viral infection and propagation for up to two viruses simultaneously can be simulated through the model, considering viruses in either the lytic or lysogenic stage, during batch, perfusion, or continuous operation. The model estimates the distribution of the viral genome(s) copy number in the cell population, which is an indicator of transduction efficiency and viral genome stability. The infection age distribution of the infected cells is also calculated, indicating how many cells are in an infection stage compatible with recombinant product expression or viral amplification. The model can also consider the presence of defective interfering particles in the system, which can severely compromise the productivity of biomanufacturing processes. Model benchmarking and validation are demonstrated for case studies of the baculovirus expression vector system and influenza A propagation in suspension cultures.

Global characterization of somatic mutations and DNA methylation changes during vegetative propagation in strawberries.

Somatic mutations arise and accumulate during tissue culture and vegetative propagation, potentially affecting various traits in horticultural crops, but their characteristics are still unclear. Here, somatic mutations in regenerated woodland strawberry derived from tissue culture of shoot tips under different conditions and 12 cultivated strawberry individuals are analyzed by whole genome sequencing. The mutation frequency of single nucleotide variants is significantly increased with increased hormone levels or prolonged culture time in the range of 3.3 × 10-8-3.0 × 10-6 mutations per site. CG methylation shows a stable reduction (0.71%-8.03%) in regenerated plants, and hypoCG-DMRs are more heritable after sexual reproduction. A high-quality haplotype-resolved genome is assembled for the strawberry cultivar "Beni hoppe." The 12 "Beni hoppe" individuals randomly selected from different locations show 4731-6005 mutations relative to the reference genome, and the mutation frequency varies among the subgenomes. Our study has systematically characterized the genetic and epigenetic variants in regenerated woodland strawberry plants and different individuals of the same strawberry cultivar, providing an accurate assessment of somatic mutations at the genomic scale and nucleotide resolution in plants.

Western Style Eastward: The Derivation of Architectural Art and Design in Harbin (1898-1945)

Harbin s architectural design is known for its fusion, with Western design providing its outer skeleton and Chinese culture giving it its inner blood. Harbin has attracted the world s attention with its unique architectural design style since the 19th century. From the early Russian and Japanese colonial period, European and Japanese styles were introduced. Harbin s architectural design history has not only witnessed the changes of an era, but also demonstrated the fusion and symbiosis of multiple cultures. In the context of globalisation, how can Chinese art participate in the process of globalisation with a unique Chinese consciousness and visual image, how to face up to the existence of exotic transplanted cultures, and how to incorporate foreign design language symbols into Chinese design methodology? These are the questions that need to be solved urgently. Harbin s architectural design is not only a display of architectural art, but also a microscopic mirror of the progress of human civilisation and cultural exchange. Through in-depth study of the philosophical thinking, cultural interactions and aesthetic concepts behind its design, we can not only have a better glimpse of the cultural concepts of urban landscape, but also have a deeper understanding of the development of human civilisation and the future direction of Chinese architectural design.

Urban Historiography and Graphic Reconstruction of a Historic Area in Valencia, Spain

The conservation of cultural heritage is a well-established fact recognized by public administrations, the scientific community, and society at large. Understanding this heritage strengthens the historical memory of communities. However, there is a type of heritage that, as it disappears or evolves over time, cannot be physically recovered: the urban fabric of historic cores. This article aims to implement a system for integrating historical urban data into a Geographic Information System (GIS) to enable the graphic recovery of urban evolution within a territory. This system facilitates the storage of alphanumeric and graphic data in a centralized database, providing a holistic view of the transformation process of a historic center’s landscape. This case study focuses on an area in the historic center of Valencia, a territory influenced by multiple cultures since the city was founded by the Romans. Each culture has altered the spatial structures within the area. Georeferenced documents from historical archives, historical cartography, and the scientific literature were utilized for this study. The results have been integrated into the current cartography of Valencia in the GIS, producing plans of successive urban stages through the overlay of historical maps and current cartography.

HeritageRoots: Developing Interconnected Immersive Experiences for Cultural Preservation and Education

In this paper, we present technical implementation details, describe the virtual world procedural generation pipeline, and discuss potential uses in cultural preservation, museum exhibits, and education of HeritageRoots, an innovative software infrastructure designed for preserving indigenous narratives and oral histories. HeritageRoots consists of two interconnected systems - (1) a web-based knowledge graph (KG) data collection and management system which stores, connects, and presents indigenous traditional knowledge (ITK) in the form of narratives, myths, and testimonies from multiple cultures and languages; (2) a virtual environment rendering pipeline which utilizes large language models (LLM), Unity3D, and Meta Quest software development kit (SDK) to procedurally generate virtual worlds from the KG data. Preliminary system evaluation with Ecuadorian Kichwa narratives showed promising results in (1) academic community interest and support, (2) the possibility of developing an infrastructure capable of connecting indigenous narratives with modern scientific knowledge via KGs and (3) procedurally generating virtual worlds from KG.

Development of efficient and scalable regeneration tissue culture method for Cannabis sativa

Large scale production of uniform disease-free plants is crucial for Cannabis sativa biotechnology. Existing micropropagation protocols rely heavily on shoot multiplication from existing meristems via direct organogenesis. Such protocols do not allow multiplication of plant material through continuous sub-culturing. Protocols that use indirect regeneration are usually not efficient enough and have very low multiplication rates. In the present study, an efficient protocol that uses a combination of direct organogenesis and callogenesis to induce multiple shoot development cultures is developed. Callogenesis was induced from various explants cultured on the media having various combinations of thidiazuron (TDZ) and naphthaleneacetic acid (NAA); best callogenesis and shoot regeneration was achieved from hypocotyl explants cultured on TDZ 0.4mgl-1 NAA 0.2mgl-1. Hypocotyls with cotyledonary node and shoot apical meristem were significantly better for shoot regeneration than explants without it. Shoots obtained from multiple shoot cultures were successfully rooted and then acclimatized under greenhouse conditions to develop into adult cannabis plants.

Fièvre Q chronique. Revue de la littérature à propos d’un cas de spondylodicite à hémocultures négatives

IntroductionFever is a cosmopolit zoonosis due to Coxiella burnetii. The diagnosis of chronic Q fever can be really misleading. The growth of this bacterium is difficult and blood cultures are often negatives. Case presentationWe rapport here the case of a 69-year-old man presenting with an alteration of his general condition and low back pain. He suffered from a well-controlled HIV infection and lower limb arteriopathy treated with a cross-femoral bypass. A computed tomography scan revealed a L3–L4 abscessed spondylodiscitis but multiple blood cultures remained sterile, and the transthoracic echocardiography was normal. PET scan showed a hypermetabolism on L3–L4 vertebrae but also indicated an intense uptake of the cross-femoral bypass. C. burnetii serology was in favour of a chronic Q fever. The management of this chronic Q fever needed a multidisciplinary discussion. Three months after the treatment initiation, C. burnetii serology was reduced by a titer and has stabilized 6months to a year. ConclusionChronic Q fever and mostly osteoarticular diseases are difficult to diagnose. We have to evoke the diagnosis of osteoarticular chronic Q fever in case of insidious inflammatory syndrome, negatives blood cultures spondylodiscitis especially when associated to endocarditis or vascular infection, and in case of spondylodiscitis with a granulomatous histology without Mycobacterium tuberculosis. Although there are many complementary tests (PET scanner, PCR), serology remains the cornerstone of diagnosis.

First detection of recombinant variant of African swine fever virus in the Russian Federation (brief communication)

As part of extensive molecular and genetic research into African swine fever virus isolates circulating in Russia, a recombinant variant with a mosaic genome structure has been identified. The one that caused an outbreak on a pig farm in the Primorsky Krai, in 2023. The characterized strain ASFV/Primorsky_2023/DP-4560.Recdemonstrates hemadsorption, active propagation in porcine primary macrophage cell culture, 99.9917% identity with the first recombinant isolates from the People’s Republic of China, recovered in 2021. Recombination sites included 79 open reading frames homologous to genotype II isolates; 49 ones homologous to genotype I and 12 mixed ones. Testing biomaterial from dead pigs in real-time polymerase chain reaction showed no changes in sensitivity or specificity, despite significant genetic distinctions between the recombinant and genotype II isolates that are enzootic to the Russian Federation. However, in 2023, D. Zhao et al. reported on high virulence of the virus related variants as revealed by the challenge tests in domestic pigs. Given the accelerating rates of AFSV molecular evolution in the East Asian countries (China, Vietnam and the Far Eastern regions of Russia), it is required to improve control measures, general and specific prevention, national and international surveillance over the economically significant animal disease.

Abstract B049: Oncolytic virotherapy as an effective tool to synergistically enhance therapeutic efficacy of standard systemic treatments for pancreatic cancer

Abstract Background: Standard therapies provide marginal benefits in overall survival and leading immunologic agents have proven ineffective in the immunologically cold environment of pancreatic ductal adenocarcinoma (PDAC). Current standard of care chemotherapies experience demonstrable resistance in the clinical setting with primary and secondary resistance frequently leading to poor outcomes. Oncolytic virus (OV) therapies are emerging as an avenue of therapeutic potential with vesicular stomatitis virus (VSV) providing an onco- selective platform with potential for rapid replication and minimal outside virulence. The aim of this project is to determine if the addition of OV to the standard systemic treatments would result in a synergistic improvement of the therapeutic efficacy, and investigate the potential mechanisms involved. Methods: A VSV variant containing the Morreton G protein inserted into a wild-type VSV backbone (VMG)was propagated and purified for in vitro and in vivo studies. In vitro studies investigating oncolytic potential and viral kinetics of VSV in combination with FOLFIRINOX (FFX) and Gemcitabine Paclitaxel (GP) therapies were compared to monotherapy in multiple cell culture formats including 2D Monolayer, 3D Spheroid, and ongoing investigation into patient derived organoids. Proteomics analysis of monotherapies along with combination therapy was performed on in vitro cultures. Ongoing in vivo studies are investigating the therapeutic potential of concurrent treatments. Results: Cytotoxicity assay studies of equivalent multiplicities of infection (MOI) demonstrated increased cytotoxicity of combination therapy (Percent viable 10.2% ± 1.3%) compared to virus alone (30.7% ± 1.9%) or FFX alone (84.4% ± 6.6%) in Hs766T PDAC cell lineAsPC-1 cell line demonstrated similar effects with minimal cytotoxicity from FFX alone (100% ± 5%) with comparable resistance to virus alone (69.9% ± 1.8%) in comparison to combination therapy (40.3% ± 1.0%). Additional cell lines including Panc-01 and MiaPaCa2 provided similar results. Treatment of formed spheroids with combination therapy resulted in reduction in overall size of spheroid along with increased Viral GFP marker fluorescence, in combination group, compared to monotherapy with each treatment. Proteomic analysis investigating concomitant treatment with both standards of care produced unique expression profiles. Multiple pathways including cell cycle regulation, apoptosis, MAPK signaling, PI3K-Akt signaling, ErbB signaling showed significant regulatory changes in protein expression across all treatment groups. Conclusion: VSV oncolytic virus in conjunction with standard of care therapy demonstrates a potentially effective approach for synergistic enhancement of the therapeutic efficacy against PDAC. Ongoing research is investigating the immunomodulatory and metabolic effects of both monotherapies in conjunction with combination therapy to further determine the synergistic mechanisms involved and how they can be further utilized to overcome treatment resistance in PDAC. Citation Format: Conner Hartupee, Amirsalar Mansouri, Joycelynn Coleman-Barnett, Dana Adamcova, Dorota Wyczechowska, Bolni Marius Nagalo, Mulu Tesfay, Jovanny Zabaleta, Luis Del Valle, John West, Jiri Adamec, Omeed Moaven. Oncolytic virotherapy as an effective tool to synergistically enhance therapeutic efficacy of standard systemic treatments for pancreatic cancer [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Pancreatic Cancer Research; 2024 Sep 15-18; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2024;84(17 Suppl_2):Abstract nr B049.

Importance of Media Composition and Explant Type in Cannabis sativa Tissue Culture.

Producing uniform Cannabis sativa (Cannabis) for medicinal/recreational flower production through sexual propagation has been problematic, leading to dominance of clonal propagation from "mother plants" in the cannabinoid industry, which also faces significant limitations. Cannabis tissue culture (TC) methods have been developed to overcome these challenges, but the long-term health and maintenance of Cannabis explants in TC have been largely overlooked in previous studies. The current study focused on the development of an efficient and optimized micropropagation protocol covering the entire process, with a specific focus on the health and performance in the multiplication stage. Multiplication media were formulated hormone-free to avoid longer-term vitrification issues, resulting in single-main-shoot cultures rather than multiple-shoot cultures. This instigated the use of stage II explant types different from the standard shoot tips previously used for multiple shoot cultures. Multiplication media were further improved from the basal salt composition via nitrogen and calcium additives. The optimized protocol was used on eight diverse Cannabis cultivars to test its applicability across various genetic backgrounds. Results indicated that the protocol was effective for conservation purposes across all cultivars and achieved good long-term multiplication rates for some but not all. The outcomes of this study mark a significant stride towards an efficient Cannabis TC methodology ready for more comprehensive industrial applications.

Acknowledging multiple ‘masculine’ organisational identities: Moving beyond the singular model of workplace safety culture to a multiple safety cultures perspective

Acknowledging multiple ‘masculine’ organisational identities: Moving beyond the singular model of workplace safety culture to a multiple safety cultures perspective

Research on the Implementation Path of Integrating Ethnic Traditional Culture into the Art Curriculum of Pre-school Education in Guangxi

In the context of the new era, with the deepening of educational reform and China's increasing emphasis on national culture, cultural heritage and educational innovation have become important topics for social development. Culture is the soul of a nation, carrying its historical memory and values. With the acceleration of globalisation, cultural diversity and cultural confidence have become particularly important. Guangxi province, as a place where Chinese ethnic minorities live, is rich in ethnic cultural resources, and these resources have great potential value in preschool education. The purpose of this paper is to explore the effective implementation path of the integration of traditional culture of ethnic minorities in Guangxi and the art curriculum of preschool education, with a view to promoting the inheritance and development of ethnic culture in Guangxi while enriching the content of preschool education and enhancing the cultural literacy of young children. By introducing the traditional culture of ethnic minorities into the art curriculum of preschool education, the content of early childhood education can be enriched, and the cognition and respect of multiple cultures can be promoted.

Leveraging the science of early life predictability to inform policies promoting child health

Addressing the tremendous burden of early-life adversity requires constructive dialogues between scientists and policy makers to improve population health. Whereas dialogues focused on several aspects of early-life adversity have been initiated, discussion of an underrecognized form of adversity that has been observed across multiple contexts and cultures is only now emerging. Here we provide evidence for “why unpredictability?”, including: 1. Evidence that exposures to unpredictability affect child neurodevelopment, with influences that persist into adulthood. 2. The existence of a translational non-human animal model of exposure to early life unpredictability that can be capitalized upon to causally probe neurobiological mechanisms. 3. Evidence that patterns of signals in the early environment promote brain maturation across species. 4. The uneven distribution of unpredictability across demographic populations that illuminates a possible focal point for enhancing health equity. We then outline the potential of unpredictability in terms of the “what”; that is, how might the concept of unpredictability be leveraged to inform policy? We emphasize the importance of interdisciplinary and community partnerships to the success of this work and describe our community-engaged research project. Finally, we highlight opportunities for the science of unpredictability to inform policies in areas such as screening, immigration, criminal justice, education, childcare, child welfare, employment, healthcare and housing.