In order to solve the problem of low analysis accuracy in the traditional method of interactive effect analysis of English spoken in classrooms in colleges and universities, a mobile learning-based method of the interactive effect analysis of spoken English is proposed. By analysing the characteristics of teacher-student interaction in a flipped classroom, a mobile learning app was constructed. To obtain the relevant information about the teacher-student interaction effect, an evaluation system of the teacher-student interaction in a college English-flipped classroom based on mobile learning was established. Combined with the fuzzy comprehensive evaluation method and multi-level gray evaluation method, the interactive effect of flipped classroom in college English-speaking was analysed according to the evaluation results. The experimental results show that the accuracy of the proposed method is up to about 99%, and the analysis time is about 4 s.