Multidimensional Scaling Research Articles

Comparative time-series analyses of gut microbiome profiles in genetically and chemically induced lupus-prone mice and the impacts of fecal transplantation

Although the association between gut dysbiosis (imbalance of the microbiota) in systemic lupus erythematosus (SLE) is well-known, the simultaneous exploration in gut dysbiosis in fecal and different intestinal sections before and after lupus onset (at 2, 4, 6, 8, and 10 months old) resulting from the loss of inhibitory Fc gamma receptor IIb (FcGIIb) and pristane induction have never been conducted. Anti-dsDNA (an important lupus autoantibody) and proteinuria developed as early as 6 months old in both models, with higher levels in FcGRIIb deficient (FcGRIIb-/-) mice. Compared to the healthy control at 2 and 4 months, the lupus mice (both FcGRRIIb-/- and pristane) and healthy mice at 6 months old demonstrated an alteration as indicated by the Shannon alpha diversity index, highlighting influences of lupus- and age-induced dysbiosis, respectively. Non-metric multidimensional scaling (NMDS) revealed that the fecal microbiota of FcGRIIb-/- mice were distinct from the age-matched healthy control at all timepoints (at 6 month, p < 0.05), while pristane mice showed divergence at only some timepoints. Analyses of different intestinal sections revealed similarity among microbiota in the cecum, colon, and feces, contrasting with those in the small intestines (duodenum, jejunum, and ileum). Subtle differences were found between FcGRIIb-/- and pristane mice in feces and the intestinal sections as assessed by several analyses, for examples, the similar or dissimilar distances (NMDS), the neighbor-joining clustering, and the potential metabolisms (KEGG pathway analysis). Due to the differences between the gut microbiota (feces and intestinal sections) in the lupus mice and the healthy control, rebalancing of the microbiota using rectal administration of feces from the healthy control (fecal transplantation; FMT) to 7-month-old FcGIIb-/- mice (the established lupus; positive anti-dsDNA and proteinuria) was performed. In comparison to FcGRIIb-/- mice without FMT, FMT mice (more effect on the female than the male mice) showed the lower anti-dsDNA levels with similar fecal microbiome diversity (16s DNA gene copy number) and microbiota patterns to the healthy control. In conclusion, gut microbiota (feces and intestinal sections) of lupus mice (FcGRIIb-/- and pristane) diverged from the control as early as 4–6 months old, correlating with lupus characteristics (anti-dsDNA and proteinuria). The different gut microbiota in FcGRIIb-/- and pristane suggested a possible different gut microbiota in lupus with various molecular causes. Furthermore, FMT appeared to mitigate gut dysbiosis and reduce anti-dsDNA, supporting the benefit of the rebalancing gut microbiota in lupus, with more studies are warranted.

Participation and Well-Being of Chinese Mothers Who Have Children With Disabilities.

High stress and compromised mental health are well-documented among mothers of children with disabilities, but less is known about the factors that influence their participation and well-being, especially among Chinese mothers. Identifying these factors could lead to strategies to mitigate potential risks to maternal well-being. To explore the relationships among participation in health-promoting activities, perceived stigma, perceived support, and well-being of ethnic Chinese mothers who have children with disabilities. Cross-sectional survey. Community. Ethnic Chinese mothers (N = 261) with children with disabilities of any age residing in Australia, Singapore, or Taiwan. Health Promoting Activities Scale, Personal Wellbeing Index, Warwick-Edinburgh Mental Wellbeing Scale; Kessler Psychological Distress Scale, Parental Perceptions of Public Attitudes Scale, and Multidimensional Scale of Perceived Social Support. Moderate to strong correlations were found between mental well-being and all measures. The frequency of participation in health-promoting activities was a predictor of mental well-being (β = .21, p = .001). Perceived support was the strongest predictor of participation in health-promoting activities (β = .25, p = .005), mental well-being (β = .39, p < .001), and personal well-being (β = .45, p < .001). Perceived stigma predicted psychological distress (β = .32, p < .001) and mental well-being (β = -0.29, p < .001). Perceived support and stigma affected mothers' participation in health-promoting activities and well-being. Therapists can facilitate mothers' engagement in health-promoting activities and develop strategies to address stigma and enhance support. Plain-Language Summary: This research highlights factors that affect the well-being of ethnic Chinese mothers who have children with disabilities and provides cultural insights for occupational therapists who work with Chinese families. The article also encourages pediatric occupational therapists to address the occupational needs and well-being of mothers in parallel with therapy for their children. We discuss occupation-focused strategies, family-centered perspectives, and health promotion approaches in relation to improving the participation and well-being of ethnic Chinese mothers in their cultural context.

Vegetation community recovery on restored bottomland hardwood forests in northeast Indiana, USA.

Vegetation communities in restored bottomland hardwood forests in northeast Indiana were studied 6-21 years after restoration to assess progress toward restoration objectives. The study focused on four sites that were restored to compensate for resource injuries after contaminant releases. The restored sites were compared with four reference-site conditions, including crops (prerestoration condition), old field communities representing a no-management alternative, locally sampled second-growth mature forests, and forest community types described by the US National Vegetation Classification (USNVC), which represent ideal or defining conditions of recognized vegetation communities. Fixed-area plots provided data on field-sampled environmental variables, vegetation, soil, and hydrological conditions for crops, old fields, restored areas, and mature forests. The USNVC database provided quantitative data for three historically and geographically relevant reference forest community types for comparison with the sampled communities. Results of nonmetric multidimensional scaling based on species cover revealed clear gradients relating to site age and canopy development. Along those gradients, restored areas demonstrated increasing similarity to mature forest reference communities in terms of floristic composition. Specifically, the floristic quality of restored areas was significantly greater than that of crops and old fields. Furthermore, soil health measurements of physical, chemical, and hydrological conditions indicated significant improvements in restored site soils compared with prerestoration conditions represented by cropland soils. Descriptions and data from the USNVC provided ecological context for restoration target conditions and facilitated the assessment of restoration recovery along a trajectory from starting conditions to those target conditions. Descriptions by USNVC also helped identify deviations from the intended restoration objectives (e.g., invasive species recruitment) and potential adaptive management actions to return sites to their intended trajectories. Integr Environ Assess Manag 2024;20:1917-1938. Published 2024. This article is a U.S. Government work and is in the public domain in the USA. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Genetic Diversity and Population Structure Analysis of Pinus elliottii Germplasm Resources in Jiangxi Province

This study aimed to compare and assess the genetic diversity and trends among the introduced family provenance, first-cycle superior trees breeding provenance, and improved-generation superior trees breeding provenance of Pinus elliottii using EST-SSR markers. The goal was to provide a foundation for advanced genetic improvement and sustainable utilization of P. elliottii in Jiangxi Province. A total of 417 individuals were analyzed for their genetic diversity and population structure using 19 pairs of SSR markers. The analysis identified 103 alleles across all the samples, with an average of 5.421 alleles per locus. Compared to other coniferous species, P. elliottii exhibited a moderate to high level of genetic diversity (I = 0.862, He = 0.457). Analysis of the molecular variance (AMOVA) revealed that 97.90% of the genetic variation occurred within provenances, consistent with a low genetic differentiation coefficient (Fst = 0.016 &lt; 0.05) and high gene flow (Nm = 15.715) among provenances. In addition, analysis using STRUCTURE v. 2.3.4 software divided the 417 germplasm samples into two distinct groups, corroborating the results of the principal coordinates analysis (PCoA) and the unweighted pair group method with arithmetic (UPGMA) clustering analysis. Overall, the germplasm resources of P. elliottii exhibited rich genetic diversity, with the majority of the genetic variation occurring within provenances. For the genetic improvement of high-resin-yielding slash pines, breeding programs should prioritize populations with high genetic diversity while carefully selecting superior individuals from within those populations. These findings provide a solid foundation for breeding high-resin-yielding varieties and for future research on the sustainable utilization of these valuable resources.

법적 판단에서의 증거평가 차원: 다차원 척도법 및 펴기의 적용

The purpose of this study was to explore the dimensions that laypersons use to evaluate evidence presented in criminal cases and to determine whether the dimensions differ according to legal decision using rankings of the importance of evidence. Multidimensional scaling was performed using the proximity matrix generated from the entire data set, and it was found that laypeople are likely to evaluate evidence according to the legal party from which it was presented (Dimension 1), while Dimension 2 may reflect the sequence of events or the objectivity of the evidence itself. The multidimensional scaling configurations for the guilty and not guilty decision were similar to the configuration of the total data, but the clustering of the evidence and the meaning of the dimensions tended to be clearer in the guilty decision configuration. When comparing vector model configurations of multidimensional unfolding using ranking data, no evidence or dimension was observed to be particularly important to those who were pro-defendant, but a majority of those who were pro-prosecution people valued the defense's evidence. Although this study is exploratory and has limited generalizability to laypersons' evidence evaluation dimensions, it is expected to provide essential knowledge for systemic understanding of the jury decision-making process.

Perfectionism and Adolescent Athletes’ Burnout: The Serial Mediation of Motivation and Coping Style

Preventing and reducing adolescent athletes’ risk of burnout can help promote long-term sports participation, improve performance, and maintain psychological well-being. The present study examined the associations between perfectionism and burnout among Chinese adolescent athletes and the mediating role of motivation and coping styles. A total of 243 Chinese adolescent athletes (78% boys; Mage = 17.8; SD = 2.62) completed the Sport Multidimensional Perfectionism Scale for China, the Situational Motivation Scale22, the Coping Scale for Chinese Athletes, and the Athlete Burnout Questionnaire to assess perfectionism (strivings and concerns), coping styles (problem-focused and emotion-focused), motivation (intrinsic and amotivation), and burnout. Path analyses indicated that intrinsic motivation and coping styles (problem-focused and emotion-focused) serially mediated the relationship between perfectionistic strivings and burnout. Problem-focused coping mediated the relationship between perfectionistic concerns and burnout. These findings contribute to a model of the effect of perfectionism on adolescent athletes’ burnout, provide support for the self-determination theory, and suggest a feasible approach for mitigating burnout in this group.

Diversity Patterns of Bacteria in the Root Zone of Davidia involucrata Along an Altitudinal Gradient

Davidia involucrata has an ancient origin, representing a remnant from the paleotropical flora that thrived during the Tertiary period. Altitudinal gradient acts as a natural testing ground for studying climate change, and research on the distribution patterns of microorganisms along altitudinal gradients is crucial in understanding the adaptability of D. involucrata to climate change. In our study, we examined sample sites ranging from 1600 to 2200 m in elevation, which are part of the primary habitat zone for Davidia involucrata within the Xuebaoding National Nature Reserve. In 2021, field surveys were conducted across four altitudinal gradients (1600 m, 1800 m, 2000 m and 2200 m) of the D. involucrata distribution in the nature reserve. The sampling plots were set in each altitudinal gradient, and three representative and healthy mature trees were selected as sample trees for each plot. Rhizosphere soils were used to test the soil stoichiometry characteristics and root zone microbial communities. Our findings indicated pronounced differences in soil total carbon (TC) and total phosphorus (TP) content and C:P and N:P ratios between the four altitude sites (p &lt; 0.05). Analysis of the bacterial communities revealed higher richness (PD and Chao1 indexes) at ASL2000 and ASL2200 (high altitude) compared to ASL1600 and ASL2000 (low altitude) (p &lt; 0.05). Non-metric multidimensional scaling analysis demonstrated a distinct clustering of bacterial communities between the high and low altitudes (p &lt; 0.01). At the phylum level, Proteobacteria and Acidobacteria were predominant at high altitudes, while Actinobacteria and Chloroflexi dominated at low altitudes. The core microbiome, shared among all altitudes, comprised 377 genes. The analysis of differential abundance revealed notable disparities in the prevalence of certain bacterial genera with altitude, with Arthrobacter and Acidothermus experiencing the most pronounced shifts (p &lt; 0.05). This confirmed that environmental factors significantly influenced bacterial community structure and abundance. Spearman’s rank correlations revealed that both Chao1 and PD indices were positively correlated with elevation, TC, and TN, with Chao1 showing stronger relationships. Both indices were negatively correlated with MAT, while only Chao1 exhibited a significant negative correlation with pH. Linear regression analysis further confirmed the significant associations between Chao1 index and elevation, TN, MAT, and pH. Furthermore, redundancy analysis demonstrated that altitude (ASL) and TN were the primary factors shaping soil bacterial community composition, explaining 21.32% and 30.70% of the variance, respectively. Altitude significantly influenced microbial community structure (p = 0.003). Distinct microbial taxa showed specific associations with environmental gradients, suggesting niche specialization in response to soil conditions. These findings suggest that altitude influences soil nutrient characteristics and microbial community composition in the D. involucrata habitat, offering insights into the ecological factors affecting this endangered species.

Biofilms in modern CaCO3-supersaturated freshwater environments reveal viral proxies

Biofilms are mucilaginous-organic layers produced by microbial activity including viruses. Growing biofilms form microbial mats which enhance sediment stability by binding particles with extracellular polymeric substances and promoting growth through nutrient cycling and organic matter accumulation. They preferentially develop at the sediment-water interface of both marine and non-marine environments, and upon the growing surfaces of modern tufa and travertine. In this context, however, little is known about the factors, environmental or anthropogenic, which affect viral communities in freshwater spring settings. To explore this issue, geochemical and metagenomic data were subjected to multidimensional analyses (Principal Component Analysis, Classical Multidimensional Scaling, Partial Least Squares analysis and cluster analysis based on beta-diversity), and these show that viral composition is specific and dependent on environment. Indeed, waters precipitating tufa and travertine do vary in their geochemistry with their viruses showing distinct variability between sites. These differences between virus groups allow the formulation of a viral proxy, based on the Caudoviricetes/Megaviricetes ratio established on the most abundant groups of viruses. This ratio may be potentially used in analysing ancient DNA preserved in carbonate formations as an additional source of information on the microbiological community during sedimentation.

Genotyping-by-sequencing shows high genetic diversity in Corylus avellana germplasm resistant to eastern filbert blight

European hazelnut (Corylus avellana) is an anemophilous, dichogamous, self-incompatible tree nut species. It is native to a large portion of Europe, Turkey, and the Caucasus region, across which a wealth of plant genetic resources is present. The objective of this study was to evaluate the genetic diversity of a core set of C. avellana representing the world’s germplasm using genotyping-by-sequencing derived single nucleotide polymorphism (SNP) markers and to classify novel eastern filbert blight (EFB) resistant or tolerant accessions. Two-hundred-twenty-two accessions underwent next-generation sequencing (NGS) to generate SNP markers. From this, 1,250 SNP markers were used to construct a neighbor-joining (NJ) dendrogram and perform a STRUCTURE and discriminant analysis of principal coordinates (DAPC) analyses. In the dendrogram, five major groups were established, which generally corresponded to geographic origins of the plant materials studied. In STRUCTURE, support was found for groupings at (K) = 3, (K) = 6, and (K) = 10 populations, with the greatest Δ-(K) value occurring at (K) = 10. Although the three different analyses indicated slightly different solutions, the overall results were generally consistent from the standpoint of identifying similar accession groupings. For many of the accessions, recorded origins tended to correspond with their genetic grouping, although there was also evidence of intermixing and likely movement of plant materials. Interestingly, in all three analyses, a vast majority of the new accessions from the Republic of Georgia formed their own distinct group, highlighting this geographic region as a unique pool of C. avellana genetic resources. Overall, EFB resistant/tolerant accessions were placed across a wide range of genetic backgrounds. Thus, our results indicate EFB resistance/tolerance is present across a wide spectrum of C. avellana genetic resources, with the Georgian accessions representing a new and relatively unique germplasm pool that can be incorporated into hazelnut breeding programs.

Utilizing SSR-based core collection development to improve conservation and utilization of Corylus L. genetic resources.

Hazelnuts are traditional woody oilseed plants. Corylus L. resources are rich in variety and widely distributed in China. However, the identification of germplasm varieties and the selection of superior varieties remain quite limited. This study aimed to analyze the genetic diversity of 331 Corylus L. germplasms using 16 simple sequence repeat (SSR) markers. Based on this, 11 pairs of core primers were selected, a fingerprint database of germplasm resources was constructed, and a primary core collection was screened. The results indicated that these tested Corylus L. germplasms exhibited a high level of genetic diversity, with an average number of alleles (Na) per locus of 14.5 and a polymorphic information content of 0.777. The phylogenetic relationships among various hazelnut cultivars were characterized by complexity, and they were delineated into four distinct groups facilitated by genetic distance analyses. An SSR fingerprint database for 331 Corylus L. germplasms was successfully constructed using the 11 obtained core SSR markers to increase the discrimination efficiency. Ultimately, 127 primary core accessions of Corylus L. were selected. The retention rate for the observed Na and MAF (the minor allele frequency) in the primary core germplasm constructed based on a sampling proportion of 38.36% was 100% and 94.7%, respectively. Shannon's information index (I) was highly consistent between the core and original germplasms, indicating that the core germplasm could fully represent the genetic diversity of the original germplasm. Additionally, the principal coordinate analysis of the selected primary core germplasm was essentially consistent with that of the entire original germplasm, further supporting the broad representativeness of the core germplasm. This study provided a basis for precisely identifying and efficiently utilizing Corylus L. accession.

Social support and mental health outcomes in palestinian children victims of bullying and cyberbullying during the covid-19 pandemic: an exploratory investigation

BackgroundStudies have shown that COVID-19 negatively impacted mental health by increasing depression and anxiety. Additionally, bullying might deteriorate children's psychological functioning. Nevertheless, social support has been studied as a positive buffer for mental well-being.ObjectivesThus, our study aimed to examine the consequences of the COVID-19 outbreak on children’s mental health when they are a victim of bullying and cyberbullying. Moreover, we tested the effect of social support as a mediating variable. A sample of 141 children (63 boys and 78 girls) 9 to 13 years old (M = 10.31, SD = 2.26) who reported having experienced bullying participated in the research. All were Palestinians living in Israel, attending primary schools.MethodsData was collected using the following measures, the Multidimensional Bullying Victimization Scale (MBVS), the Adolescents Cyber-Victimization Scale (CYBVICS), the Strengths and Difficulties Questionnaire (SDQ), a questionnaire measuring the effect of COVID-19 on mental health outcomes, the Revised Child Anxiety and Depression Scale (RCADS), and the Multidimensional Scale of Perceived Social Support (MSPSS). Structural equation modelling was applied to test the association between variables and mediating effects.FindingsA positive correlation between Fear of COVID-19 and traditional bullying, cyberbullying, depression, and anxiety were found. Moreover, social support was negatively correlated with traditional forms of bullying and cyberbullying. Also, statistically significant differences between traditional bullying, cyberbullying, depression, and parents' difficulties emerged from parental marital status.Conclusionsfindings might help victims of bullying by training teachers and guiding parents to construct intervention plans to empower the social networks of children victims.

Validation of the Multidimensional Scale of Perceived Social Support among LGBT population in Serbia

Abstract Background Social support represents the belief that one is valued, cared for, and loved by others in a social network and a lack of it may lead to adverse outcomes such as a relapse into depression or emotional distress in physically ill patients. The Multidimensional Scale of Perceived Social Support (MSPSS) is an instrument aimed to measure an individual’s perception of how much he or she receives outside social support. This study aimed to examine the validity and reliability of the Serbian version of MSPSS in a sample of LGBT population in Serbia. Methods The cross-sectional study included 504 respondents, members of the LGBT population living in Serbia. The instrument used was the MSPSS as a self-report measure, containing twelve items rated on a 7-point Likert scale. MSPSS consists of three sub-scales: Family, Friends, and Significant Others. Exploratory (EFA) and confirmatory factor analyses (CFA) were performed with two independent random samples. Results The average score on the scale of social support was 5.58±0.84 (scale of social support by family 4.62±1.41, scale of social support by friends 5.93± 1.99, and scale of social support by the significant others 6.20±0.96, respectively). The Cronbach’s alpha for the entire social support scale was α = 0.772. The three-factor structure of the MSPSS was proved with EFA and CFA. The factor loadings varied between 0.630-0.817. The three factors explained a total of 52.68% of the variance (factor 1- 28.62%, factor 2. 13.16%, factor 3- 10,90%). The three-factor model was adopted during the CFA when the three-factor model CFI (comparative fit index) was 0.969. Conclusions The MSPSS has shown good reliability, internal validity and construct validity and can be used as a convenient measure of social support among the LGBT population. Both EFA and CFA yield a three-factor structure consistent with the theory underlying the measure’s development. Key messages • The results of the study suggest that the Multidimensional Scale of Perceived Social Support has acceptable psychometric properties for use with the LGBT population. • This study supports the use of the MSPSS for assessing community-based collaboration using the three-factor model among the LGBT population.

Effects of Cognitive Flexibility, Prosocial and Problem Behaviours, Parenting Style and Social Support on Social Adaptation Among Children With Leukaemia: A Structural Equation Modelling Approach.

Leukaemia children often encounter various social adaptation issues stemming from changes in their living environment. Identifying and optimising the factors associated with social adaptation is crucial in improving social adaptation in children with leukaemia. To investigate social adaptation in children with leukaemia and to clarify the impact pathway of cognitive flexibility, problem behaviour, prosocial behaviour, parenting style and social support on social adaptation. Using a convenience sampling method, 264 leukaemia children completed a cross-sectional survey. Data were collected using the Social Adaptation Assessment Scale for Children with Leukaemia, Strengths and Difficulties Questionnaire, Cognitive Flexibility Scale, Short-Egna Minnenav Barndoms Uppfostran and Multi-Dimensional Scale of Perceived Social Support. Structural equation modelling was used to test the influencing factors of social adaptation. Social support directly influenced social adaptation (β = 0.19, p < 0.01) and indirectly influenced social adaptation through prosocial behaviour (β = 0.06, p < 0.01) and problem behaviour (β = 0.18, p < 0.01). Prosocial behaviour (β = 0.17, p < 0.01) and problem behaviour (β = -0.61, p < 0.01) directly influenced social adaptation. Negative parenting directly influenced social adaptation (β = 0.12, p < 0.01) and indirectly influenced social adaptation (β = -0.23, p < 0.01) through problem behaviour. Our finding indicated that there are prominent social adjustment problems in the recovery process of children with leukaemia. These findings suggest that targeted education is needed to enhance social adaptation among children with leukaemia. The final model should serve as a framework to guide intervention research targeting social support and negative parenting to improve the social adaptation of children with leukaemia. During the recovery process, nurses need to regularly follow up on the children's social adaptation and encourage them to participate in social activities to increase social support while providing family guidance based on improving parenting styles. The subjects of this study were children with leukaemia aged 8-18 years. All authors read and approved the final manuscript.

Genetic Diversity and Association Analysis of Traits Related to Water-Use Efficiency and Nitrogen-Use Efficiency of Populus deltoides Based on SSR Markers

Populus deltoides is one of the primary tree species for bioenergy production in temperate regions. In arid/semi-arid northern China, the scarcity of water and nitrogen significantly limits the productivity of poplar plantations. The identification of relevant molecular markers can promote the breeding of resource-efficient varieties. In this study, 188 genotypes of P. deltoides from six provenances served as experimental material. Genetic differentiation analysis, analysis of molecular variance (AMOVA), principal coordinate analysis (PCoA), unweighted pair group method with arithmetic mean (UPGMA) clustering, and genetic structure analysis were performed using selected simple sequence repeat (SSR) markers. Based on these analyses, the association analysis of water-use efficiency (WUE) and nitrogen-use efficiency (NUE) were conducted using general linear model (GLM) and mixed linear model (MLM) approaches. The results showed that 15 pairs of SSR primers successfully amplified across all 188 individuals, with an average of 7.33 alleles (Na) observed per primer pair. The polymorphism information content (PIC) ranged from 0.060 to 0.897, with an average of 0.544, indicating high genetic diversity in the selected markers. The average inbreeding coefficient intra-population (Fis), inbreeding coefficient inter-population (Fit), and inter-population genetic fraction coefficient (Fst) values were 0.005, 0.135, and 0.132, respectively, indicating high heterozygosity, substantial inbreeding within populations, and moderate genetic differentiation, with an average gene flow (Nm) of 1.964, suggesting substantial gene flow between populations. Additionally, molecular variance was primarily within individuals (84.12%). Genetic structure analysis revealed four subgroups, with some degree of genetic admixture among the provenances. In the GLM model, 11 markers were significantly associated with five traits (p &lt; 0.05), with an average contribution rate of 15.82%. Notably, SSR132 and SSR143 were significantly associated with multiple traits (p &lt; 0.05). The MLM model identified two markers (SSR47 and SSR85) significantly associated with ground diameter (p &lt; 0.05) and one marker (SSR80) significantly associated with NUE (p &lt; 0.05). This study identifies loci associated with WUE and NUE, laying a foundation for future genetic improvement and marker-assisted breeding strategies in poplar.

The assessment of the perceived stress and the quality of life in the patients with myasthenia gravis: The mediating role of the psychological capital and social support

Background: Stress has been known as a risk factor for the onset and modification of autoimmune disorders such as myasthenia gravis (MG). However, the patients can control their stress and improve their quality of life (QOL) using some factors such as psychological and social support. Psychological capital and social support play a key role in decreasing stress and improving QOL in the patients with MG. Therefore, the present study aimed to assess the mediator role of the psychological capital and social support in perceived stress and QOL of the patients with MG. Methods: In the present cross-sectional study, 203 patients with MG, including 138 women and 65 men, were selected from Iran Myasthenia Gravis Association and Shariati Hospital, Tehran City, Iran, using available sampling. The selected patients completed the Myasthenia Gravis Quality of Life questionnaire (MG-QOL), Luthans Psychological Capital Questionnaire (PCQ), Multidimensional Scale of Perceived Social Support (MSPSS), and Cohen Perceived Stress Scale (PSS-14). To assess the mediator role of the psychological capital and the perceived social support in the relationshipwith the perceived stress and QOL of the patients with MG, structural equation modeling (SEM) was used. Results: There was a significant negative relationship between the perceived stress and the variables of psychological capital (including hope, resilience, optimism, and self-efficacy), perceived social support dimensions (including support from important people, support from family, and support from friends), and QOL dimensions (including social activity and mental health) (P &lt; 0.01). The results also showed that the perceived social support dimensions (including support from important people, support from family, and support from friends) and the variables of psychological capital (including hope, resilience, optimism, and self-efficacy) had a significant positive relationship with the QOL in patients with MG (P &lt; 0.01). The indirect effect of perceived stress on the QOL through social support and psychological capital was 0.16 and 0.15, respectively, which was statistically significant (P &lt; 0.05). Conclusion: The present study results show that a part of the shared variance between the conceptual circles of the perceived stress and QOL in patients with MG results from variability in the psychological capital and social support.

The Occurrence of Myxomycete Communities in Taxodium distichum Forests: Influence of Substrates and Seasons

Myxomycete communities in Taxodium distichum forests and the influence of substrates and seasons on myxomycete diversity were studied. In total, 80 substrates (40 living barks and 40 litters) were collected from the sample site in winter, spring, summer and autumn of 2023, followed by moist chamber cultures. A total of 733 records representing 34 myxomycete species in 21 genera were obtained. Arcyria cinerea and six other species were abundant (A). Six species were common (C), six species were occasional (O), and fifteen species were rare (R). Species diversity (H’ = 2.04 ± 0.20) of corticolous communities was significantly higher than that (H’ = 1.49 ± 0.31) of litter-inhabiting communities (p &lt; 0.05). Nonmetric Multidimensional Scaling (NMDS) analyses showed significant differences between these two communities (p &lt; 0.01). Species diversity was slightly higher in summer and autumn compared to winter and spring. NMDS analyses indicated no significant differences among seasonal communities. Redundancy Analysis showed that the corticolous species were closely related to wind speed (p &lt; 0.05), while litter-inhabiting species were mainly determined by temperature (p &lt; 0.05). The study revealed that substrates and seasons influenced myxomycete communities in T. distichum forests, with the communities being particularly affected by the substrates in which they live.

Comparative Analysis of Intestinal Morphology and Gut Microbiota of Spinibarbus sinensis Under Different Aquaculture Systems

Fish gut health is influenced by various factors, with the environment being a significant one. S. sinensis is a key aquaculture species in China, yet research on the impact of different aquaculture systems on its intestinal health remains limited. This study aims to explore the changes in intestinal morphology and gut microbiota of S. sinensis under two aquaculture systems. The juveniles of S. sinensis were divided into two groups and cultured in traditional ponds (CT) and an in-pond tank culture system (JY), with equal amounts of feed provided daily over a 72-day experimental period. The results showed no significant differences in growth performance metrics, including the specific growth rate, weight gain rate, hepatosomatic index, and viscerosomatic index between the two groups. In terms of intestinal morphology, the JY group villus width was significantly wider than the CT group, and the number of goblet cells in the CT group was significantly higher than that of the JY group (p &lt; 0.05), which suggested that the fish in the JY group may have better intestinal nutrient absorption capacity, while the water quality in the CT group may be worse. The 16S rRNA gene sequencing analysis of the gut microbiota showed that the JY group had a significantly higher Shannon index compared to the CT group (p &lt; 0.05), indicating greater species richness and evenness. Principal Coordinates Analysis (PCoA) revealed a distinct clustering of gut microbiota between the two groups. At the phylum level, the relative abundance of Fusobacteriota was significantly higher in the CT group, whereas Bacteroidota and Proteobacteria were significantly higher in the JY group (p &lt; 0.05). Furthermore, KEGG pathway predictions indicated differences in the potential metabolic capabilities of the gut microbiota between the two groups (p &lt; 0.05). Overall, this study is the first to conduct a comparative analysis of the growth performance, intestinal tissue morphology, and gut microbiota of S. sinensis under two different aquaculture systems, which has valuable implications for the further optimization of aquaculture practices.

Short-term effects of co-applied biochar and paper mill biosolids on soil microbial communities under field conditions

Biochar and paper mill biosolids (PB) are reported to improve soil fertility, crop growth and indirectly conditions for soil microbial communities. However, it is unclear how the co-application of these materials impacts soil microbial communities under field conditions. A study was initiated in 2018 in Québec, QC, Canada and fine roots and rhizosphere soil were sampled to determine the effects of co-application of wood biochar (0, 10, and 20 Mg dry wt. ha−1) and PB (0 and 30 Mg wet wt. ha−1) on the percentage of corn (Zea mays L.) and soybean (Glycine max L. Merr.) root colonization by arbuscular mycorrhizal fungi (AMF), soil microbial biomass phosphorus (MBP) and microbial (Bacteria, Fungi and AMF) diversity and community structure in a temperate loamy soil. Co-applying PB and biochar increased soil MBP compared with the control and biochar-only application. Applying PB alone or with biochar increased the level of root colonization by AMF compared with the control and biochar-only application in soybean but not in corn. Overall, biochar and PB application had no significant effect on bacterial and AMF diversity and community structure compared with the control. However, applying PB alone or with biochar decreased the fungal alpha diversity (Shannon and Simpson indices), affected several fungal taxa abundances and shifted the fungal community structure as indicated by the principal coordination analysis (PCoA). Our results provided an understanding on the short-term effects of co-applied wood biochar and PB on microbial communities of a temperate loamy soil under field conditions, as well as scientific bases for further investigation.

Jazz hands and jitters: Exploring valence and arousal dimensions with multidimensional scaling techniques

Utilizing the two-dimensional plane of valence and arousal with multi-dimensional scaling (MDS), the current study sought to better understand the structure of affect and the relationship between emotion items. The positive and negative affect schedule (PANAS) is the most frequently applied self-report measure of affect but has been criticized for only including high arousal emotions. A between-subjects, two-part experimental study was conducted. Participants (N = 548) were recruited online and asked to rate the similarity between one of two target words (anger: n = 271; calm: n = 277) and 143 emotion words. Our results provided evidence for the two-dimensional plane of valence and arousal, indicating that all four quadrants are important when studying affect. Overall, the current study found support for the activation-arousal theory of affectivity with MDS analyses. Emotion words clustered in all four quadrants of the two-dimensional plane, demonstrating the necessity of an affect measure that encompasses all four quadrants. Furthermore, our results demonstrated that the PANAS does not include items in the positive valence/low activation quadrant, however, a newly published measure was found to have increased item dispersion, with items included in all four quadrants of affect. Limitations and proposed future directions are discussed.

Multiple dimensions of immorality

ABSTRACT We conducted a four-part study to map out the conceptual space of a diverse set of immoral items, including those that are extreme and/or intergroup (e.g. child sex abuse, genocide, slavery), with the goal of identifying attributes spontaneously used in moral judgment. In Part 1, we identified 56 immoral items. In Part 2, participants completed a similarity-based card sort task of the 56 immoral items. Multidimensional scaling (MDS) indicated that three-dimensional space was needed to capture the perceived differences among the items. In Part 3, regression analysis indicated that perceived similarity among the immoral items related to their commonness, objectivity, forgivability, and legality. In Part 4, regression analysis indicated that the configuration of immoral items corresponded to the amount of anger and disgust the items elicited and items’ perceived harmfulness. We attempt to synthesize these results and answer questions about the roles of anger, disgust, and harm in moral judgment.