The present study investigates the impact of glow discharge cold plasma-induced duoplasmatron ion source on the mechanical properties of multi-axial knitted structure reinforced glass fiber composites. Analysis of failure location and mode in bending tests was conducted using the acoustic emission method. The results reveal that the tensile and bending strength of the multi-axial structure reinforced glass fiber composites treated with oxygen/nitrogen plasma had increased by 21.84% and 37.28%, respectively. In contrast, when treated with oxygen/argon plasma, the corresponding enhancements were 17.47% and 24.16%, respectively. These results indicate that oxygen/nitrogen plasma treatment yields superior mechanical properties to oxygen/argon treatment. The accumulated acoustic emission energy in the multi-axial structure reinforced glass fiber composites subjected to oxygen/nitrogen and oxygen/argon treatments was 25.49% and 62.39% lower than untreated samples, respectively. The failure mode observed in the untreated composites was matrix cracking, whereas the composites treated with dual plasma exhibited interface debonding. The dual plasma modification enhanced fiber wettability and surface roughness, facilitating increased contact area between the fiber and matrix and promoting the overall bonding performance between the two components. Taken together, our findings suggest that duoplasmatron ion source treatment enhanced the interface bonding and mechanical properties of the multi-axial structure reinforced glass fiber composites.