In mixed C3/C4 grasslands of the southern Great Plains of North America, honey mesquite (Prosopis glandulosa Torr.) invasion facilitates the dominance of the C3 midgrass Texas wintergrass (Nassella leucotricha [Trin. & Rupr.] Pohl) to the detriment of warm-season grasses. Little is known about the viability of targeted grazing as a treatment method for reducing Texas wintergrass biomass and reproduction, particularly under mesquite canopies. We quantified the effects of targeted grazing treatments (grazed once [G1], grazed twice [G2], and control) and mesquite canopy position (under canopy [UC] and outside of canopy [OC]) on Texas wintergrass basal area, total dry matter (DMY) per basal area, live DMY per basal area, dead DMY per basal area, leaf length, total number of reproductive culms per plant, number of fungus-infected reproductive culms per plant, and rate of reproductive culm development over 2 yr at two study sites. Targeted grazing reduced leaf length and biomass and slowed reproductive development of the wintergrass plants that cattle could more easily access. Environmental factors and differences in mesquite physiognomy at the two study sites likely interacted with grazing treatments, making it challenging to separate the impacts of targeted grazing treatments from other factors. Results suggest mesquite removal is likely necessary to increase cattle access to Texas wintergrass, particularly in stands with multistemmed trees.