The enormous variety and specificity of cell functions observed in vivo are the result of multifaceted interplays occurring between adjacent cells or between cells and the extracellular environment. Arrays of signals whose presentation is tightly controlled in time and space regulate different aspects of cell behavior including differentiation, quiescence, proliferation and biosynthesis [1]. The integration of such a complex set of information within synthetic platforms has acquired large interest from the scientific community in order to fabricate biomaterials and devices that precisely control cell functions and fate in vitro. The field of functional biointerfaces is widely interdisciplinary, spanning from biochemistry, molecular and cell biology, microand nanotechnologies, material synthesis and functionalization. The successful translation of the scientific findings into the engineering of devices for practical biotechnological applications relies on integrating those disciplines. In this context, diverse but complementary contributions need to be integrated into new biomaterials, that is, multisignal patterning methodologies, multiscale modeling, advanced characterization and processing technologies. These should be optimized and match the desired biomedical or biotechnological applications. Thus, the concept of functional biointerfaces is rapidly evolving and constantly gaining new valences [2,3]. When a cell lands on a material surface, an integrin-mediated material recognition occurs and clusters of integrins assemble in the form of punctuate-wide nascent adhesions (<0.2 μm). If the surface characteristics are favorable, in terms of ligand stability and density, these adhesions can mature in larger focal complexes up to focal adhesions (FAs, 1.0–10.0 μm) [4]. The actomyosin cytoskeleton is anchored to the adhesion that provides a mechanical continuity with the extracellular environment by transmitting forces from the inside of the cytoplasm and vice versa. Therefore, patterned surface features, that is, topography, biochemical ligands or mechanical properties, with dimensions that interfere with the establishment and dynamics of the adhesion process, can control the mechanical identity of the cytoskeleton and such a pathway is usually referred to as the material–cytoskeleton crosstalk [5]. In this context, the capability of manipulating material properties at the nanoscale is key central. Surfaces can be nanoengineered in order to affect adhesion plaque size, orientation and shape, thus affecting the mechanical identity of the cell through its cytoskeletal assembly. Additionally, contractile forces generated by the cytoskeleton may alter nuclear stress state and shape, which has a direct influence on chromatin dynamics [6,7]. Therefore, chromatin might directly perceive material features through the FAs–cytoskeleton– Nanoengineered materials to control cell fate
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