Abstract This paper builds a cultivation system of innovation and entrepreneurship ability, and puts forward the corresponding cultivation mode and implementation path. At the same time, under the implementation conditions, a multiple linear regression model is used to explore the influence of the integration of aesthetic education on innovation and entrepreneurship ability. The results show that aesthetic interest, aesthetic perception, innovation consciousness and innovation and entrepreneurship ability cultivation are significantly positively correlated, with correlation coefficients of 0.039, 0.025, 0.12 respectively. Multiple covariate diagnosis shows that the model fit is good, the value of VIF is more than 3, and that the influence of aesthetic interest, aesthetic perception, and innovation consciousness on college students’ entrepreneurial intention is significant. In addition, regression analysis showed that the standardized regression coefficient of aesthetic interest on the cultivation of innovation and entrepreneurship was −0.113 (P<0.001), and the standardized regression coefficient of innovation awareness was −0.128, which indicated that aesthetic education had a significant favorable influence on the cultivation of innovation and entrepreneurship. The study verifies the effectiveness of the innovation and entrepreneurship ability cultivation system incorporating aesthetic education. It provides data support for implementing innovation and entrepreneurship education in colleges and universities.