Multigene Phylogenetic Analyses Research Articles

Identification and biological characteristics of Fusarium tobaccum sp. nov., a novel species causing tobacco root rot in Jilin Province, China.

Fusarium wilt of tobacco (FWT), caused by Fusarium spp., has emerged as a severe threat to tobacco production in China. In all, 132 isolates of Fusarium were isolated from tobacco and pathogenic to tobacco-causing FWT in Jilin Province, China. In this study, we identified 7 of 132 isolates as a novel species Fusarium tobaccum sp. nov. Zhao Xie & Jie Gao, using multi-gene phylogenetic analyses of translation elongation factor (tef1), β-tubulin (tub2), calmodulin (cmdA), and RNA polymerase II second largest subunit (rpb2) genes, along with subtle morphological differences. Isolates of F. tobaccum sp. nov. were clustered in a distinct branch in the maximum parsimony phylogenetic tree generated from the sequences of tef1-rpb2-tub2-cmdA and can be distinguished from closely related species F. cugenangense, F. callistephi, and F. elaeidis. The morphological characteristics of F. tobaccum sp. nov. are distinct from other Fusarium species. F. tobaccum sp. nov. exhibits abundant aerial mycelia and pigment production on potato dextrose agar (PDA), microconidia with 0-1 septa, and macroconidia with 2-5 septa on carnation leaf-piece agar (CLA), and produces abundant chlamydospores on Spezieller Nährstoffarmer agar (SNA) and CLA. The mycelia of F. tobaccum exhibited optimal growth at a pH of 7.1 and a temperature of 23.6°C. Sucrose and NaNO3 significantly promoted the mycelial growth of F. tobaccum. PD medium was optimal for total sporulation. However, the sporulation ratios of the macrospores of F. tobaccum in PD, SN, and CMC were relatively low (0.48%, 2.51%, and 2.16%, respectively). These findings provided valuable insights into the morphological and biological characteristics of F. tobaccum.IMPORTANCEFusarium wilt of tobacco (FWT) is a prevalent issue in tobacco-growing regions globally, leading to significant losses in yield and quality. This study identified F. tobaccum sp. nov., a novel species of Fusarium causing FWT in China. The identification was based on multi-gene phylogenetic analyses and morphological characteristics. The effects of temperature, pH, carbon source, nitrogen source, medium, and light on the mycelial growth of F. tobaccum sp. nov. were determined. These findings might contribute to future research on the pathogenic mechanisms of this novel species and the development of strategies to control FWT.

Koima, a new genus of nemacheilid loach from the Western Ghats, India (Cypriniformes: Nemacheilidae)

Koima, a new genus of nemacheilid loach, is described for Mesonoemacheilus remadevii Shaji 2002, and Nemacheilus monilis Hora 1921, both of which are endemic to the Western Ghats of southern peninsular India. Koima is distinguished from all other genera of family Nemacheilidae by its unique colour pattern comprising a yellowish-brown ground colour, single row of black spots on lateral line, all fins hyaline, and absence of a uniform banding pattern on dorsal side. Koima remadevii was initially identified as a species of Mesonoemacheilus Bănărescu & Nalbant 1982, from which it can be distinguished by the presence of a lower adipose crest, and an absence of a banding pattern on the dorsal side and on the caudal fin, while Koima monilis was initially identified as a species of Nemacheilus Bleeker 1863, from which it can be distinguished by the presence of a lower adipose crest, a narrow and deeply incised upper lip and having the fins hyaline. In the multi-gene phylogenetic analysis based on concatenated mitochondrial and nuclear gene sequences, Koima remadevii and K. monilis were recovered in a monophyletic group, sister to the endemic peninsular Indian nemacheilid, Schistura denisoni. Resolution of the identity of Koima remadevii and K. monilis contributes to our understanding of the phylogenetics of family Nemacheilidae, which is widely considered to be a polyphyletic group.

Three New Species and Five New Host Records from Chaetomiaceae with Anti-Phytopathogenic Potential from Cover Crops Astragalus sinicus and Vicia villosa

Cover crops, typically planted during off-seasons and requiring less agronomic manipulation, may provide abundant fungal resources. Certain species of Chaetomiaceae could serve as potential agents for controlling plant diseases and developing bioorganic fertilizers. Eight species from five genera of Chaetomiaceae were identified from healthy Astragalus sinicus and Vicia villosa, two major cover crops, through multigene phylogenetic analysis, morphological identification, and pairwise homoplasy index testing. The identified species comprise three new species: Achaetomium astragali, Subramaniula henanensis, and S. sichuanensis, as well as five known but new host record species: Botryotrichum murorum, Chaetomium coarctatum, C. pseudocochliodes, C. pseudoglobosum, and Collariella pachypodioides. Dual culture tests revealed that isolates of all eight Chaetomiaceae species exhibited antagonistic effects on multiple phytopathogens. Among the identified fungi, the NSJA2 isolate, belonging to C. coarctatum, exhibited significant relative inhibition effects on 14 out of 15 phytopathogens tested in this study, indicating its broad-spectrum antagonistic effects. Additionally, NSJA2 exhibited excellent salt tolerance. Overall, our study has identified multiple fungi with anti-phytopathogens potential, among which NSJA2 exhibits high potential for practical application. This finding paves the way for further exploration and exploitation of NSJA2 as a promising biocontrol agent.

Amphisphaeria hongheensis sp. nov. (Amphisphaeriaceae, Amphisphaeriales) from Yunnan Province, China

Yunnan Province encompasses the highest fungal diversity in China, and novel fungal species are regularly introduced. Several saprobic fungi were collected during surveys of fungi associated with woody plants in Yunnan Province, Southwestern China. The fungi samples were initially identified as Amphisphaeria. Multi-gene phylogenetic analysis based on combined datasets of LSU, ITS, rpb2, and tub2 regions and morphological characteristics confirmed that the samples represent a previously unidentified fungus belonging to Amphisphaeria, which is named A. hongheensis sp. nov. This paper provides a full description, illustrations, and a phylogenetic tree showing the new species' taxonomic placement.

Additions to the genus Kirschsteiniothelia (Dothideomycetes); Three novel species and a new host record, based on morphology and phylogeny.

During a survey of microfungi associated with forest plants, four specimens related to Kirschsteiniothelia were collected from decaying wood in Guizhou, Hainan and Yunnan Provinces, China. Kirschsteiniothelia species have sexual and asexual forms. They are commonly found as saprophytes on decaying wood and have been reported as disease-causing pathogens in humans as well. In this study, we introduce three novel Kirschsteiniothelia species (K.bulbosapicalis, K.dendryphioides and K.longirostrata) and describe a new host record for K.atra, based on morphology and multi-gene phylogenetic analyses of a concatenated ITS, LSU and SSU rDNA sequence data. These taxa produced a dendryphiopsis- or sporidesmium-like asexual morph and detailed descriptions and micromorphological illustrations are provided. Furthermore, we provide a checklist for the accepted Kirschsteiniothelia species, including detailed host information, habitat preferences, molecular data, existing morphological type, country of origin and corresponding references.

Catalogue of fungi in China 2. Ramaria from northern China

ABSTRACT The species of Ramaria are coralloid-fungi with intricately branched and vividly coloured basidiomata. There are many studies on the genus Ramaria in China, but molecular phylogenetic analysis is rarely employed. In this study, we performed a multigene phylogenetic analysis to identify Ramaria species in Shanxi Province of northern China. Phylogenetic analyses based on four loci, the internal transcribed spacer region of nuclear ribosomal DNA (ITS), the nuclear large subunit ribosomal DNA (nrLSU), ATPase subunit 6 (atp6), and mitochondrial small subunit ribosomal DNA (mtSSU), revealed 13 Ramaria species from our collections. Combined with morphological examinations, 12 of them were identified as new species, plus a new report to China. The thirteen species were described and illustrated in this paper.

Redefining phylogenetic placements of Hyphodiscosia: integrative taxonomic insights based on morphological and phylogenetic analyses

In conducting a survey on the diversity of lignicolous freshwater fungi across a north-south latitudinal gradient in the Asian region, two strains were collected from freshwater habitats. This species is characterized by forming pinkish sporulating colonies both on wood and in culture, presenting macronematous conidiophores that swollen at the apex, producing cylindrical, pink in mass, 1-septate conidia, each with two flexuous, filamentous appendages arising from the middle of the cells. These characteristics closely match the definition of Hyphodiscosia. Through multigene phylogenetic analysis, this study for the first time determines the phylogenetic position of Hyphodiscosia within Muyocopronaceae (Muyocopronales). This study provides detailed illustrations, updates on molecular data, and a morphological comparison with related genera within Muyocopronaceae, as well as discusses the validity of the currently accepted four species within Hyphodiscosia.

Trichoderma thailandense sp. nov. (Hypocreales, Hypocreaceae), a new species from Thailand

A new species of microfungus, described herein as Trichoderma thailandense was isolated from the pileus surface of oyster mushroom and upland black rice samples in Thailand. This species is described using morphological characteristics and molecular multi-gene analyses. Based on multi-gene phylogenetic analyses of the internal transcribed spacer (ITS) region of the rDNA, RNA polymerase subunit II gene sequences (rpb2), and translation elongation factor 1-α (tef1-α) gene revealed that the position of T. thailandense is well-supported within Harzianum clade of the genus Trichoderma, but distinct from other all other known species. A full description, illustrations and a phylogenetic tree showing the position of T. thailandense are provided.

New Occurrence of Nigrospora oryzae Causing Leaf Blight in Ginkgo biloba in China and Biocontrol Screening of Endophytic Bacteria

Ginkgo biloba is a multifunctional composite tree species that has important ornamental, economic, medicinal, and scientific research value. In October 2023, the foliage of G. biloba on the campus of Nanjing Forestry University exhibited leaf blight. Black-brown necrotic spots were observed on a large number of leaves, with a disease incidence of 86%. After isolating a fungus from symptomatic leaves, pathogenicity was tested to satisfy Koch’s postulates. Using morphological features and multi-gene phylogenetic analyses of an internal transcribed spacer (ITS), elongation factor 1-alpha (EF1-α), and beta-tubulin (β-tub), the isolates YKB1-1 and YKB1-2 were identified as Nigrospora oryzae. N. oryzae was previously reported as an endophyte of G. biloba. However, this study shows it to be pathogenic to G. biloba, causing leaf spots. Two endophytic bacteria were isolated from asymptomatic leaves of diseased G. biloba trees, and their molecular identification was performed using 16S ribosomal DNA (16S rDNA). GBB1-2 was identified as Bacillus altitudinis, while GBB1-5 was identified as Bacillus amyloliquefaciens. The screening and verification of endophytic bacteria provide a new strategy for the control of N. oryzae.

First Report of Alternaria alternata Causing Leaf Spot of Magnolia delavayi in China

Magnolia delavayi, known as Delavay's magnolia or Chinese evergreen magnolia, is an enchanting deciduous tree native to southwestern China (Xu et al. 2020). In March 2024, leaf spots were observed on M. delavayi plants at Qujing Normal University (25.527°N, 103.744°E), Qujing, Yunnan, China. About 80% of the trees (n=92) on campus were infected, with infected leaves accounting for about 10 to 30% of all leaves. The leaf spots exhibited a round or oval shape, with a yellowish-brown center and a brown outer ring. As the spots expanded, the leaves gradually dried up and fell off. Five symptomatic leaves were collected from the middle sections of five diseased plants. Tissue samples (5 × 5 mm) were excised from the lesion margins and subjected to surface sterilization in 75% ethanol for 30 seconds and 1% sodium hypochlorite for 60 seconds. They were then rinsed three times with sterile water and dried on sterilized paper. The samples were plated on potato dextrose agar (PDA) and incubated at 28 °C for 3 days. Developing colonies were retransferred to new PDA and purified by the hyphal tip technique (Senanayake et al. 2020). Two representative isolates (SYL1 and SYL2) were selected and confirmed to be the same species based on morphological characteristics and molecular identification. On PDA, the fungal colonies initially appeared grayish-white and turned dark gray over time with cotton-like aerial hyphae on the surface. The conidia are light brown, pear-shaped or rod-shaped, with 1 to 4 transverse septa and 0 to 1 oblique septa, and measured 5.4 to 12.7 × 10.4 to 25.7 μm (n = 50). Molecular identification was performed by partial sequencing of the internal transcribed spacer (ITS: ITS4/ITS5) region, glyceraldehyde 3-phosphate dehydrogenase (GAPDH:gpd1/gpd2), translation elongation factor 1-alpha (TEF1:EF1/EF2), and Alternaria major allergen (Alt a 1:Alt-for/Alt-rev) (Woudenberg et al. 2015). The resulting sequences of ITS (PP951882, PP951883), GAPDH (PP968942, PP968943), Alt a 1(PP968940, PP968941) and TEF1 (PP968938, PP968939) were deposited in GenBank. BLAST analyses revealed that the sequences from these two isolates showed 100% identity with those from Alternaria alternata strains for each gene. Additionally, a multigene phylogenetic analysis revealed a distinct group containing SYL1, SYL2 and other A. alternata strains with a posterior probability of 100%. The morphological and phylogenetic evidence collectively suggested that the two isolates belonged to A. alternata. Strain SYL1 was used for the pathogenicity test on three 5-year-old M. delavayi plants on campus. Three healthy leaves of each plant were punctured with a sterile needle and sprayed with 20 μl of spore suspension (1 × 106 conidia/ml). Similarly, another nine wounded leaves were sprayed with 20 μl of sterile water. All inoculated leaves were enclosed in plastic bags for 48 hours. Disease symptoms were observed on leaves inoculated with spore suspension after 7 days, while control plants remained healthy. This experiment was repeated three times with same results. To fulfill Koch’s postulates, A. alternata was reisolated from the inoculated leaves and identified based on morphological characteristics and ITS sequencing. To our knowledge, this is the first report of A. alternata causing leaf spot on M. delavayi in China. This research expands the known host range of this pathogen and provides relevant information to the design of disease management strategies.

Morpho-phylogenetic evidence reveals Pseudobionectria phoenicis gen. et sp. nov. (Bionectriaceae) from a mangrove habitat

A bionectriaceous ascomycete was collected from palm rachides in a mangrove habitat. Multi-gene phylogenetic analyses indicated this fungus belongs within the Bionectriaceae, forming a sister lineage to Septofusidium herbarum and distinct from other known genera. In this study, we proposed the new genus Pseudobionectria to accommodate the novel species P. phoenicis. Pseudobionectria is distinguished from other genera in Bionectriaceae by its superficial, globose, brownish-orange ascomata with a black crown that is flattened on the top and hyphal appendages that merge with the subiculum below. The asci are unitunicate, lacking a visible apical ring, and the ascospores are 1-septate, oblong-ellipsoidal to fusiform, hyaline, and exhibit spinose ornamentation. The establishment of this new genus is supported by morphological and phylogenetic evidence. Additionally, this paper provides a synopsis of the genera within Bionectriaceae, including information on the type species, morphological characteristics, lifestyles, molecular data and key publications.

Novel characterisation of Curvularia geniculata associated with leaf blight on Costus igneus in India

Costus igneus Nak, commonly referred as insulin plant or spiral flag, is a perennial herb belonging to the family Costaceae. Owing to its ability to regulate the blood sugar level, it is considered as a boon plant for diabetic patients, hence popularly known as the “sugar plant”. Although few foliar diseases have been documented on C. igneus, they generally occur in minor frequency under Indian conditions. However, in May 2022, a moderate to severe incidence of leaf blight was observed on insulin plants at University of Agricultural Sciences, GKVK, Bangalore, and nearby medicinal gardens. The affected leaves exhibited light brown lesions coalesced to form papery blight, gradually leading to complete drying of leaves. The current study aimed to identify, characterize, and document the fungal pathogen responsible for the disease, with a focus on its morphological, molecular, and biological characteristics to ensure accurate identification. Additionally, the study sought to determine optimal conditions for pathogen growth and sporulation to support the development of effective disease management strategies. The associated fungus was isolated, purified and identified as Curvularia geniculata through morphological and multigene phylogenetic analysis, using internal transcribed spacer (ITS) and large sub unit (LSU) regions of rDNA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Biological characterization revealed that cornmeal agar (CMA) at 30 °C and pH of 7 were ideal for better growth and abundant sporulation. These findings, highlighting the emergence of C. geniculata as a novel fungal pathogen infecting C. igneus in India, are crucial for developing effective disease management strategies to safeguard the medicinal properties of the plant.

New Helminthosporium (Massarinaceae, Dothideomycetes) and Nigrospora (Incertae sedis, Sordariomycetes) species associated with walnut (Juglansregia L.) in China.

Six collections of ascomycetes were obtained from samples collected from dead branches and leaves of Juglansregia in Guizhou and Yunnan provinces, China. By incorporating multigene phylogenetic analysis (ITS, LSU, rpb2, SSU, tef1-α, tub2) supplemented by morphological data, we establish two novel species, namely Helminthosporiumguizhouense and Nigrosporayunnanensis. In morphology, H.guizhouense can be distinguished from H.caespitosum by its narrower conidia (13-16 µm vs. 27.3-35.5 µm), and N.yunnanensis is characterized by black, globose conidia (16.2 × 14.4 µm). The phylogenetic results further substantiated them as novel taxa. The present study contributes to our comprehension of the range of fungi found in Juglansregia, thereby expanding our knowledge of the diversity of fungi within this host.

Foliar blight, leaf spot, and stem blackening diseases caused by Stemphylium beticola, Alternaria alternata, and Alternaria atra on dragon's head (Lallemantia iberica) in Iran

Dragon's head, Lallemantia iberica, is an oilseed crop adapted to be grown in cold-dry areas. In the growing season of 2022–23 a severe foliar blight, leaf spot, and stem blacking symptoms were observed in commercial fields in northern and northwestern Iran, leading to more than 50 % yield loss. Infected samples were collected and transferred to the laboratory. Following the isolation of fungal agents, five representative isolates were selected for further studies. Morphological studies, based on conidial shape, conidiation, and other mycological characteristics, were carried out on a pure culture established from a single spore. Furthermore, multigene phylogenetic analyses were performed using sequences of translation elongation factor 1-α (TEF1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), RNA polymerase II second largest subunit (RPB2), and Internal Transcribed Spacer (ITS) rDNA genes for Alternaria isolates, along with calmodulin (cmdA) for the Stemphylium isolate. The morphological and molecular studies identified the fungal isolates as Alternaria atra, Alternaria alternata, and Stemphyliium beticola. Pathogenicity tests on the commercial cultivar ‘Sara’ indicated that all isolates were pathogenic to dragon's head, but they differed significantly in aggressiveness. To the best of our knowledge, this is the first report of the association of these fungal species with an important foliar disease on this crop in Iran and worldwide.

Novel endophytic fungal species Pithoascus kurdistanensis producing morphine compounds

Papaver genus, commonly known as popies, is a valuable source of alkaloids used in medicine, including papaverine, morphine, codeine, and thebaine. We isolated six endophytic fungal isolates producing morphinan alkaloids from four Papaver species growing in Kurdistan Province, Iran. To do this, a 1:1 mixture of methanol and chloroform was used to extract fungal cultures. The contents of morphinan alkaloids in the extracts were subsequently determined using phase high-performance liquid chromatography. Among the morphinan alkaloid-producing fungal isolates, IRAN 4653C had the highest yield giving 23.06 (mg/g) morphine and 2.03 (mg/g) codeine when grown in potato dextrose liquid medium. The identity of this isolate was examined and recognized as a new fungal species named as Pithoascus kurdistanesis sp. nov. based on multi-gene phylogenetic analyses of ITS, TEF-1α, and TUB2 sequences data and morphological features. The morphinan-producing endophytic fungus and the isolated Pithoascus species from Papaver are being reported for the first time. Accordingly, this fungus shows promise as a new source of valuable compounds which is illustrated and introduced here as a new Microascaceae member belonging to Pithoascus from Kurdistan Province, Iran. Moreover, the morphinan productivity of P. kurdistanesis was further validated by gas chromatography-mass spectrometry (GC–MS).

Fusarium species associated with Euwallacea xanthopus in South Africa, including two novel species

ABSTRACT Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) are small wood-boring insects that live in an obligate symbiosis with fungi, which serve as their primary food source. Beetles residing in the genus Euwallacea have evolved a unique association with a clade of Fusarium that falls within the aptly named Ambrosia Fusarium Clade (AFC). The discovery of the invasive polyphagous shot hole borer, E. fornicatus, in South Africa, has heightened awareness of ambrosia beetles and their symbionts in the country. In this study, we investigated the Fusarium symbionts of three species of Euwallacea in South Africa, with a specific focus on those associated with E. xanthopus. Isolations of Fusarium strains from both living and dissected beetles yielded nearly 100 isolates. Using multigene phylogenetic analyses, these isolates were identified as six different Fusarium species. Fusarium hypothenemi and F. euwallaceae have previously been reported from South Africa. Fusarium pseudensiforme and Fusarium AF-6 are new records for the country. The remaining two species are new to science and are described here as F. rufum sp. nov. and F. floriferum sp. nov. Targeted fungal isolation from specific beetle body parts revealed that the AFC species collected were typically associated with the dissected beetle heads and helped us identify the likely nutritional symbiont of E. xanthopus. This study highlights the understudied diversity of fungal associates of ambrosia beetles present in South Africa.

Insights from multigene analysis: first report of a Southeast Asian Mosquito, Aedes (Mucidus) laniger (Diptera: Culicidae) on Jeju Island from Korea

BackgroundCertain mosquitoes are known as dominant vectors worldwide, and transmit infectious diseases. The expansion of mosquito habitats due to climate change and increased human activities poses a significant health threat by facilitating the spread of various non-native infectious diseases. This study focused on the detection of the Southeast Asian mosquito species, Aedes (Mucidus) laniger (Wiedemann, 1820) on Jeju Island, the southernmost region of the Republic of Korea (ROK), highlighting the potential risks associated with the spread of vector-borne diseases, particularly emphasizing the elevated likelihood of invasion by Southeast Asian mosquitoes.MethodsField surveys were conducted in August 2023 on Jeju Island. Adult mosquitoes were collected using BG-sentinel traps and identified to the species level using taxonomic keys. Morphological and molecular analyses were employed to confirm species designations. Molecular data, including mitochondrial and nuclear genes, were used for phylogenetic analysis, which was performed to compare and identify among recorded subgenera in ROK. Species distribution modeling for Ae. laniger was performed to predict potential habitats using R package ‘BIOMOD2’.ResultsThe two specimens of Ae. laniger were collected for the first time on Jeju Island. Morphological and molecular analyses confirmed the identity of this species within the subgenus Mucidus and validated the first record of this species in the ROK. We employed a simple multigene phylogenetic analysis to confirm a new mosquito record at the genus and subgenus levels, finally validating the consistency between morphological identification and molecular phylogenetic outcomes. Furthermore, we have updated the taxonomic keys for the genus Aedes in the ROK, and revised mosquito lists for Jeju Island, incorporating the inclusion of Ae. laniger. On the basis of species distribution modeling, the area of suitable habitat for Ae. laniger is expected to expand due to climate change, but this change did not appear to be meaningful in East Asia.ConclusionsThis case offers the first report of the Southeast Asian mosquito, Ae. laniger, in the ROK. The detection of this species on Jeju Island suggests the potential establishment of a breeding population their habitat and raises concerns about further expansion into the Korean Peninsula. Considering the annual occurrence of mosquito-borne disease cases in the Southeast Asia, it is essential to conduct monitoring not only in Jeju Island, where Ae. laniger has been identified, but also across the entire Korean Peninsula.Graphical

Multi-Gene Phylogenetic Analyses Reveals Heteroxylaria Gen. Nov. and New Contributions to Xylariaceae (Ascomycota) from China.

An in-depth study of the phylogenetic relationships of Xylaria species associated with nutshells of fruits and seeds within the genus Xylaria and related genera of Xylaceaecea was conducted in China. The multi-gene phylogenetic analyses were carried out based on ITS, RPB2, and TUB sequences of 100 species of 16 known genera in Xylariaceae around the world. Based on molecular phylogenetic analyses, morphological observations, and ecological habitats, a new genus, Heteroxylaria, is established to accommodate four new species, viz. H. cordiicola, H. juglandicola, H. meliicola, and H. terminaliicola, and four new combinations, viz. H. oxyacanthae, H. palmicola, H. reevesiae, and H. rohrensis. The genus is characterized by cylindrical stromata with conspicuous to inconspicuous perithecial mounds, surface black, having brown to dark brown ascospores with a germ slit, and it grows on nutshell of fruits. The combined ITS+RPB2+TUB sequence dataset of representative taxa in the Xylariaceae demonstrate that Heteroxylaria is grouped with Hypocreodendron but forms a monophyletic lineage. All novelties described herein are morphologically illustrated and compared to similar species and phylogeny is investigated to establish new genera and species.

Broadening the horizon of Indian mycobiota: two new species and one new generic record in Boletoid mushrooms (Boletaceae)

A combination of morphological studies and multigene molecular phylogenetic analyses unveiled two significant novel species of boletes namely Anthracoporus indohimalayanus sp. nov. and Boletus dhakuricus sp. nov. from the states of Sikkim and Uttarakhand, respectively. The first novel species, i.e. A. indohimalayanus is also a new generic record of the genus Anthracoporus from India, whereas the second novel species i.e. B. dhakuricus is a member of wild edible porcini mushroom. Both the taxa are described with their morphological details and molecular phylogenetic inferences.

Two new species of Nectriaceae (Hypocreales, Sordariomycetes) from Yunnan, China.

Nectriaceae is a highly diverse family, and members have a worldwide distribution, particularly in warm temperate to tropical regions. During the survey of fungal diversity in different habitats in Yunnan province, China, two new species isolated from soil and air respectively, namely Atractiumyunnanense sp. nov. and Nalanthamalaxishuangbannaensis sp. nov., were proposed based on morphological comparisons and the multi-gene phylogenetic analyses of combined ITS, LSU, rpb2, and tub2 sequence data. Phylogenetically, both species clustered in a monophyletic clade within Nectriaceae with strong support. A.yunnanense is characterized by synnematous conidiophores, pale olivaceous-green, clavate to oblong-ellipsoidal, multi-septate conidia, and pale olivaceous-green chlamydospores. N.xishuangbannaensis has acremonium-like or penicillium-like conidiophores and either obovate or ellipsoidal, cylindrical or fusiform conidia. Full descriptions, illustrations, and a phylogenetic tree showing the phylogenetic position of the two new species were provided.