Mulberry Species Research Articles

The Effect of Phenolic-Rich Extracts of Rubus fruticosus, R. ulmifolius and Morus nigra on Oxidative Stress and Caco-2 Inhibition Growth.

Currently, a clear interest has been given to berries due to their richness in active metabolites, including anthocyanins and non-coloured phenolics. Therefore, the main aim of the present work is to investigate the phenolic profile, antioxidant abilities, and antiproliferative effects on normal human dermal fibroblasts (NHDF) and human colon carcinoma cell line (Caco-2) cells of phenolic-rich extracts from three red fruits highly appreciated by consumers: two species of blackberries (Rubus fruticosus and Rubus ulmifolius) and one species of mulberry (Morus nigra). A total of 19 different phenolics were identified and quantified by HPLC-DAD-ESI/MSn and HPLC-DAD, respectively. Focusing on the biological potential of the phenolic-rich extracts, all of them revealed notable scavenging abilities. Concerning the antiproliferative properties, R. fruticosus presented a cytotoxic selectivity for Caco-2 cells compared to NHDF cells. To deeper explore the biological potential, combinations with positive controls (ascorbic acid and 5-fluorouracil) were also conducted. Finally, the obtained data are another piece of evidence that the combination of phenolic-rich extracts from natural plants with positive controls may reduce clinical therapy costs and the possible toxicity of chemical drugs.

Elemental Characterization of Leaf Extracts of Three Different Species of Mulberry: Morus. alba L., Morus. nigra L. and Morus. indica L., Using Inductively Coupled Plasma -Atomic Emission Spectroscopy (ICP-AES)

The present study aimed to compare the elemental composition of leaf extracts from three different plant species of Mulberry, Morus alba L., Morus nigra L., and Morus indica L. The results showed that ten essential elements of biological importance for human metabolism, such as Copper, Chromium, Iron, Manganese, Sodium, Zinc, Calcium, Nickel, Lead, and Cadmium, were present in varying concentrations, well below the World Health Organization, (WHO's) daily intake limits. Mulberry leaves are an economical and readily available source of essential mineral elements vital for human health, which can be used to fortify functional foods, treat various diseases, and act as nutraceuticals. The information obtained could be used to determine the efficacy and dosage of herbal drugs manufactured from the leaf extracts of the Mulberry plant, making it a new contributor to food supplements and nutraceutical products.

Mulberry: A Multipurpose Plant

Abstract: Mulberry, a member of the Moraceae family, is regarded as a special plant because of its wide geographic distribution across continents, its adaptability to different cultivation methods, and the various uses of its leafy foliage. It has several benefits for environmental safety measures like soil erosion prevention, bioremediation of polluted sites, water conservation, and air quality improvement through carbon sequestration. The Mulberry is also employed as a medicinal plant to improve and enhance the quality of human life. The potential antibacterial, anti-inflammatory, antioxidant, antihyperglycemic, anti-cancer, and anti-tyrosinase inhibitory action of mulberry extracts from the leaf, stem, root, and bark have been documented. Mulberries are a great source of nutrients and phytochemicals with potent medicinal properties, making them a highly nourishing food for humans. The mulberry fruit is a key ingredient in many value-added products, such as jams, jellies, wines, and syrups, since it is high in carbohydrates, protein, and dietary fibre. Mulberry leaves are an excellent source for creating of protein-rich food products with built-in antioxidants. Although Mulberry is produced professionally for sericulture in many nations, its potential for use as a human food source is not well known. The review discusses the value of mulberry as a source for various food, cosmetics, beverage, and pharmaceutical industries. All of the crucial mulberry species were compiled in this review article to evaluate it as the perfect plant for ensuring a better future.

Thermochemical characterization of different mulberry species (Morus spp.) for bioenergy production

Thermochemical characterization of different mulberry species (Morus spp.) for bioenergy production

Minor millets based agroforestry of multipurpose tree species of Bhimal (Grewia optiva Drummond J.R. ex Burret) and Mulberry (Morus alba L.) for resource conservation and production in north western Himalayas – 10-year study

In a long term study 2009–2018 at ICAR-IISWC, Dehradun, the uniqueness of minor millets based agroforestry systems (AFSs) for effective soil water conservation and biomass production in foot hills of Himalayas were evaluated. The Morus alba (Ma) based AFS was found to be the most productive and profitable system producing 1.1 and 1.8 t ha−1yr−1 of finger millet and wheat grain, respectively besides carbon stocks of 22.9 t ha−1. Morus based agroforestry system produced the highest yield of green fodder and dry fuel wood at 5.1 and 4.5 t ha−1yr−1, respectively after 10 years of planting. The reduction in minor millets yield was offset by the additional production of tree fodder and dry fuel wood by the Multiparous trees (MPTs) in the AFSs.The minimum soil loss was recorded inMa + barnyard millet (BM) based AFS followed byMa + finger millet (FM) at 15.4 and 16.4 t ha−1yr−1 in comparison to cultivated fallow at 25.6 t ha−1. The traditional millets recorded the identical soil loss of 19.7 t ha−1 during the period. The MPTs land use recorded the soil loss of 22.4–22.8 t ha−1. Similarly, the lowest run off was recorded in millet-AFSs ranging from 23.0% to 25.6% than cultivated fallow without crops (38.9%). The highest soil and runoff conservation efficiency was observed in order of AFSs> sole millets > MPTs > cultivated fallow.Millet based AFS led to the improvements in soil physical properties and fertility status significantly.Ma + FM based AFS recorded favourable changes in bulk density (B.D.), water stable aggregates and organic carbon, OC, 0.73%. Grewia optiva + BM recorded the highest OC (0.75%) besides plant nutrients P (183.2%) and K (51.1%) over the cultivated fallow. Agroforestry systems of Ma+FM-wheat and Ma+FM-toria were the most productive and robust systems economically in respect of benefit: cost ratio and net present value.

Genome size, genetic diversity, and phenotypic variability imply the effect of genetic variation instead of ploidy on trait plasticity in the cross-pollinated tree species of mulberry.

Elucidation of genome size (GS), genetic and phenotypic variation is the fundamental aspect of crop improvement programs. Mulberry is a cross-pollinated, highly heterozygous tree eudicot, and comprised of huge ploidy variation with great adaptability across the world. However, because of inadequate information on GS, ploidy-associated traits, as well as the correlation between genetic and phenotypic variation hinder the further improvement of mulberry. In this present research, a core set of 157 germplasm accessions belonging to eight accepted species of Morus including promising functional varieties were chosen to represent the genetic spectrum from the whole germplasm collection. To estimate the GS, accessions were subjected to flow cytometry (FCM) analysis and the result suggested that four different ploidies (2n = 2x, 3x, 4x, and 6x) with GS ranging from 0.72±0.005pg (S-30) to 2.89±0.015pg (M. serrata), accounting~4.01 fold difference. The predicted polyploidy was further confirmed with metaphase chromosome count. In addition, the genetic variation was estimated by selecting a representative morphologically, diverse population of 82 accessions comprised of all ploidy variations using simple sequence repeats (SSR). The estimated average Polymorphism Information Content (PIC) and expected heterozygosity showed high levels of genetic diversity. Additionally, three populations were identified by the model-based population structure (k = 3) with a moderate level of correlation between the populations and different species of mulberry, which imply the effect of genetic variation instead of ploidy on trait plasticity that could be a consequence of the high level of heterozygosity imposed by natural cross-pollination. Further, the correlation between ploidies, especially diploid and triploid with selected phenotypic traits was identified, however, consistency could not be defined with higher ploidy levels (>3x). Moreover, incite gained here can serve as a platform for future omics approaches to the improvement of mulberry traits.

Blackberries and Mulberries: Berries with Significant Health-Promoting Properties.

Blackberries and mulberries are small and perishable fruits that provide significant health benefits when consumed. In reality, both are rich in phytochemicals, such as phenolics and volatile compounds, and micronutrients, such as vitamins. All the compounds are well-known thanks to their medicinal and pharmacological properties, namely antioxidant, anti-inflammatory, anti-cancer, antiviral, and cardiovascular properties. Nevertheless, variables such as genotype, production conditions, fruit ripening stage, harvesting time, post-harvest storage, and climate conditions influence their nutritional composition and economic value. Given these facts, the current review focuses on the nutritional and chemical composition, as well as the health benefits, of two blackberry species (Rubus fruticosus L., and Rubus ulmifolius Schott) and one mulberry species (Morus nigra L.).

Genome size, chromosome number variation and its correlation with stomatal characters for assessment of ploidy levels in a core subset of mulberry (Morus spp.) germplasm

The large size of the germplasm collection along with scanty information on their cytological and genome constitution have hindered well-planned breeding schemes in mulberry. To address the issue, a study was undertaken to investigate the variability in DNA content and genome size, chromosome number, ploidy and its relation with important stomatal characteristics among 162 mulberry germplasm collection. These germplasm comprise a core subset of 150 collections along with a representative collection of different mulberry species including the wild. Among the germplasm belonging to 16 species, we identified 122 diploids (2n = 28), 4 aneuploids (2n = 30), 13 triploids (2n = 42), 15 tetraploids (2n = 56), 7 hexaploids (2n = 84) and 1 dodecosaploid (2n = 308) based on the chromosome count. Most of the cultivated mulberries are found to be diploids. The mean nuclear 2C DNA content estimated by Flow cytometry, varied from 0.723 ± 0.006 pg (M. australis, 2n = 2x) to 7.732 pg (M. nigra, 2n = 22x). The 2C DNA content positively correlated with the ploidy status and stomatal length (r = 0.814, p < 0.001). Based on the 1Cx value, the study also suggests that the majority of the polyploid species have experienced genome downsizing in relation to their diploid progenitors. This study provides the most essential information on chromosome number, ploidy and DNA content to facilitate the utilization of a core subset of germplasm in the mulberry breeding program.

The gap-free genome of mulberry elucidates the architecture and evolution of polycentric chromosomes.

Mulberry is a fundamental component of the global sericulture industry, and its positive impact on our health and the environment cannot be overstated. However, the mulberry reference genomes reported previously remained unassembled or unplaced sequences. Here, we report the assembly and analysis of the telomere-to-telomere gap-free reference genome of the mulberry species, Morus notabilis, which has emerged as an important reference in mulberry gene function research and genetic improvement. The mulberry gap-free reference genome produced here provides an unprecedented opportunity for us to study the structure and function of centromeres. Our results revealed that all mulberry centromeric regions share conserved centromeric satellite repeats with different copies. Strikingly, we found that M. notabilis is a species with polycentric chromosomes and the only reported polycentric chromosome species up to now. We propose a compelling model that explains the formation mechanism of new centromeres and addresses the unsolved scientific question of the chromosome fusion-fission cycle in mulberry species. Our study sheds light on the functional genomics, chromosome evolution, and genetic improvement of mulberry species.

Composition of Proteins and Phenolics in the Leaves of Different Mulberry Species (Morus alba L., M. alba × rubra, M. australis Poir., M. nigra L.)

The leaves of the mulberry (Morus sp.) have a variety of medicinal, culinary, industrial and agricultural applications. In our study, we compared the protein and phenolic contents of different mulberry species (Morus alba L., M. alba × rubra, M. australis Poir., M. nigra L.) from the mulberry germplasm collection to determine species-specific differences. The possibility of using mulberries as animal feed and for pharmacological purposes was reviewed. Total phenols of all genotypes were analysed using the Folin-Ciocalteu method, while total protein content was determined using the Lowry's method. The individual phenols were analysed by high-performance liquid chromatography with UV/VIS detection. The total protein content ranged from 162.03 mg BSA/g DW (M. australis) to 239.42 mg BSA/g DW (M. alba). Significantly higher contents of total proteins were determined in the leaves of M. alba. The highest mean concentrations of total phenols (21.51 mg GAE /g DW), chlorogenic acid (18.05 mg/g DW), 4-caffeoylquinic acid (4.36 mg/g DW), 5-p-coumaroylquinic acid (2.01 mg/g DW), quercetin glycoside (0.74 mg/g DW) and kaempferol acetyl hexoside (4.42 mg/g DW) were determined in M. alba × rubra and M. nigra. In contrast, white mulberry (M.alba) genotypes contained on average the most rutin (2.63 mg/g DW) and quercetin-malonyl-hexoside (1.59 mg/g DW). It can be concluded that the leaves of the white mulberry are best suited as animal feed due to their high protein content, while the black mulberry and the hybrid M. alba × rubra have pharmacological potential due to their high phenolic content.

Black mulberry (Morus nigra L.) fruits: As a medicinal plant rich in human health-promoting compounds

Abstract Morus nigra, black mulberry, is the most attractive among mulberry species. The study aimed to determine human health-promoting content and antifungal activity in fruits of seven black mulberry genotypes grown in the Tortum district of Turkey. The genotypes exhibited significant differences in most of the human health-promoting content. Among individual sugars and organic acids, glucose (6.98–8.03 g/100 g fresh weight base) and malic acid (6.33–10.45 g/100 g fresh weight base) were predominant. The ascorbic acid content of genotypes was found between 18.13 and 26.77 mg/100 g fresh weight base, indicating that black mulberry fruits had moderate ascorbic acid content. Total phenolic content, total antioxidant capacity, and total anthocyanin contents in fruit extracts of seven black mulberry genotypes were also investigated. The results showed that the levels of the aforementioned parameters changed depending on genotypes. The total phenolic and total anthocyanin contents were in the range of 1,656–2,348 μg gallic acid equivalent (GAE)/g and 623–804 μg C3GE (cyanidin-3-glucoside equivalent)/g, respectively. The genotypes had antioxidant capacity between 17.41 and 3.86 μg/mL and between 10.08 and 14.11 μmol TE/g measured by the 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assay, respectively. Some of the genotypes (TOR-1 and TOR-2) had high antifungal activity particularly against C. albicans. It was determined that the people living in the region traditionally use mulberries as blood enhancer, immune system booster, and mouth lesion treatments to protect themselves against different types of cancers and against inflammation. The present results confirm that attractive fruits of black mulberry are a rich natural source of phenolic antioxidants and can contribute to the dietary intake of antioxidants, depending on genotypes.

A review on nutritional composition and effects of drying methods of different mulberry species

A review on nutritional composition and effects of drying methods of different mulberry species

Estimation of Some Phytochemical Compounds and Antioxidant Properties of Leaves from Different Mulberry Varieties Grown in Syria.

The study was conducted to compare the chemical composition and bioactive compounds of leaves from different mulberry species grown in Syria (Morus alba, Morus nigra, Morus rubra, and Morus nigra sp). The leaves were collected in July 2022 and their proximate composition (moisture, ash, lipids, and protein), total polyphenol content (TPC), ascorbic acid, and antioxidant activity, as measured by ferric reducing antioxidant power (FRAP), were investigated. The results showed that the moisture, ash, and fat content ranged from 61.84 to 71.17%, 12.87 to 17.61%, and 3.82 to 9.68% dry weight (DW), respectively. The phenolic concentration of leaves from four different mulberry cultivars varied significantly, from 164.9 (Morus alba) to 461.5 gallic acid equivalents mg/100 g DW (M. nigra). The antioxidant activity of mulberry leaves ranged from 122 to 166.3 meq ascorbic acid/100 g DW and was arranged as follows: M. rubra > M. nigra sp > M. nigra > M. alba. According to the findings, mulberry leaves could be used to create new food supplements, functional foods, and medical applications.

Screening and Structure-Activity Relationship for Selective and Potent Anti-Melanogenesis Agents Derived from Species of Mulberry (Genus Morus).

Tyrosinase is a multifunctional, copper-containing and rate-limiting oxidase that catalyses crucial steps in the melanogenesis pathway and is responsible for skin-pigmentation abnormalities in mammals. Numerous tyrosinase inhibitors derived from natural and synthetic sources have been identified as an objective for the development of anti-melanogenesis agents. However, due to side effects and lack of expected efficiency, only a small percentage of them are used for medical and cosmetic purposes. This critical review focuses on searching for novel active substances and recently discovered plant-derived anti-tyrosinase inhibitors from the Morus genus (Moraceae family). A detailed analysis of their structure-activity relationships is discussed. The information contained in this article is crucial for the cosmetics and medical industries, in order to show new directions for the effective search for natural anti-melanogenesis products (with satisfactory efficiency and safety) to treat and cure hyperpigmentation.

Полиморфизм отборной формы шелковицы в Северо-Западном регионе России

Morus alba L. is one of the fruiting mulberry species. Morphologically, mulberry is a fast-growing deciduous, perennial tree with a deep root system, growing in Central Asia, North India, Afghanistan, Iran, the Caucasus, Turkey, China and many other countries. This culture is valuable because of the high content of antioxidants. The object of study is a selected form of mulberry from the park-arboretum of the BIN RAS. grown in St. Petersburg, the selected form has an average fruit length of 17.56 mm, a width of 10.21 mm, a weight of 13.80 g and a sugar content of 12.5 Brix. It was found that the variability of the length and width of the leaf is at an average level - 20 and 25%, and the length of the petiole is at a low level (17%). The leaves of 89% of the sample are lobed, and the rest with whole blades. Keywords: SELECTIVE FORM, FRUITS, MULBERRY, GROWING

Transcriptome and metabolome analyses reveal the efficiency of in vitro regeneration by TDZ pretreatment in mulberry

Thidiazuron (TDZ) is a plant growth regulator that is widely used as a defoliant and cytokinin-like compound. Additionally, mulberry is an economically important crop. However, regeneration is difficult in most mulberry species, and the regulatory mechanism controlling the regeneration process remains unclear. In this work, an efficient in vitro shoot regeneration system for mulberry was established by TDZ application. The results showed that the optimum adventitious bud induction, 70.83% in tetraploid M. notabilis cv. Chuanbei1 (CB1) and 96.82% in M. abla cv. Yunhua (YH), was obtained by the shoot pretreatment method using petiolate leaves as explants. The high-frequency regeneration petiolate leaf explants of YH treated with TDZ (T8) and the control (CK) were subjected to multiomic analyses. The comparison of the TDZ-treated with the CK groups identified 494 upregulated differentially expressed genes (DEGs) and 293 downregulated DEGs. GO analysis indicated that these DEGs were related mostly to integral component of membrane, transcription factor activity, and phosphorelay signal transduction system. Metabolomic analysis identified 1,889 compounds, including 205 differentially accumulated metabolites (DAMs). Of these DAMs, the number of lipids and lipid-like molecules was highest and accounted for 27.8% of all DAMs. Transcriptome profile, metabolomic, and phytohormone integrated analyses revealed that TDZ rearranged adenine-type cytokinins (CTKs) and negatively regulated endogenous CTK signal transduction. Moreover, TDZ treatment enhanced auxin signal transduction by increasing the expression of auxin flux carrier genes and downstream regulated genes without changing auxin biosynthesis in mulberry. In summary, this study provides a new method for mulberry in vitro regeneration and contributes to a better understanding of the transcriptional and metabolic changes that occur in mulberry after TDZ treatment.

Chromosome-level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants

Multiple plant lineages have independently evolved sex chromosomes and variable karyotypes to maintain their sessile lifestyles through constant biological innovation. Morus notabilis, a dioecious mulberry species, has the fewest chromosomes among Morus spp., but the genetic basis of sex determination and karyotype evolution in this species has not been identified. In this study, three high-quality genome assemblies were generated for Morus spp. [including dioecious M. notabilis (male and female) and Morus yunnanensis (female)] with genome sizes of 301–329 Mb and were grouped into six pseudochromosomes. Using a combination of genomic approaches, we found that the putative ancestral karyotype of Morus species was close to 14 protochromosomes, and that several chromosome fusion events resulted in descending dysploidy (2n = 2x = 12). We also characterized a ∼ 6.2-Mb sex-determining region on chromosome 3. Four potential male-specific genes, a partially duplicatedDNA helicase gene (named MSDH) and three Ty3_Gypsy long terminal repeat retrotransposons (named MSTG1/2/3), were identified in the Y-linked area and considered to be strong candidate genes for sex determination or differentiation. Population genomic analysis showed that Guangdong accessions in China were genetically similar to Japanese accessions of mulberry. In addition, genomic areas containing selective sweeps that distinguish domesticated mulberry from wild populations in terms of flowering and disease resistance were identified. Our study provides an important genetic resource for sex identification research and molecular breeding in mulberry.

The Ethylene Response Factor ERF5 Regulates Anthocyanin Biosynthesis in 'Zijin' Mulberry Fruits by Interacting with MYBA and F3H Genes.

Ethylene promotes ripening in fruits as well as the biosynthesis of anthocyanins in plants. However, the question of which ethylene response factors (ERFs) interact with the genes along the anthocyanin biosynthesis pathway is yet to be answered. Herein, we conduct an integrated analysis of transcriptomes and metabolome on fruits of two mulberry genotypes (‘Zijin’, ZJ, and ‘Dashi’, DS, with high and low anthocyanin abundance, respectively) at different post-flowering stages. In total, 1035 upregulated genes were identified in ZJ and DS, including MYBA in the MBW complex and anthocyanin related genes such as F3H. A KEGG analysis suggested that flavonoid biosynthesis and plant hormone signaling transduction pathways were significantly enriched in the upregulated gene list. In particular, among 103 ERF genes, the expression of ERF5 showed the most positive correlation with the anthocyanin change pattern across both genotypes and in the post-flowering stages, with a Pearson correlation coefficient (PCC) of 0.93. Electrophoresis mobility shift assay (EMSA) and luciferase assay suggested that ERF5 binds to the promoter regions of MYBA and F3H and transcriptionally activates their gene expression. We elucidated a potential mechanism by which ethylene enhances anthocyanin accumulation in mulberry fruits and highlighted the importance of the ERF5 gene in controlling the anthocyanin content in mulberry species. This knowledge could be used for engineering purposes in future mulberry breeding programs.

Molecular Assessment of Established Clonal Propagated Mulberry (Morus nigra L.)

Mulberry (Morus Sp.) is one of the economically important trees cultivated for the tasteful fruits, its several pharmaceutically important chemicals potential, timber, cosmetic and in silk industry for its foliage, also in various molecular breeding applications. The origins of most cultivated mulberry varieties are believed to be in the area of China-Japan and in the Himalaya foothills. and it now has a very wide distribution range in Asia-Europe (from Korea to Spain, including China, India, Central Asia, and Near East); in Africa (North and East Africa) and in America (from the U.S.A. to Argentina, including Mexico, Central America, Colombia and Brazil). In Saudi Arabia, Mulberry grown well and spread in different places such as Taif Province (El Shafa region), Eastern region; Al-Ehsaa Province and some southern areas. Nodal explants of Morus nigra were clonally propagated in vitro for plant regeneration. Auxiliary shoot buds have been promoted in Murashige and Scoog (MS) media in a variety of cultural contexts. The largest number of shoots (13.00 ± 0.47) with an average length of 2.00 ± 0.47 cm was initially obtained from a medium containing 2.0 mg / L N6-benzyladin (BA) and 3% sucrose. Recurrent subcultures provided the highest number of seedlings (approximately 29.30) for excavation after the fourth passage. Seedlings were rooted in 1/2 MS medium supplemented with 1.0 mg / 1 indole-3-butyric acid (IBA). Successfully, about 90% of the plantlets acclimatized. Along with the determination of the genetic variations between three mulberry genotypes including two cultivated accessions (Morus alba) and one wild genotype (M. nigra) utilizing inter-simple sequence repeat (ISSR) markers. Genetic variation and phylogenetic relationship of mulberry germplasm collection have been studied. All ISSR markers used in this study revealed higher genetic diversity in the wild species comparing with cultivated species. ISSR matrices reported that the mean genetic similarity coefficient was 0.7677 for all mulberry genotypes. Although some differences have been observed, much similarity has been obtained in dendrogram topology. Cluster analysis of the ISSR using UPGMA software revealed that wild species were genetically distinct. The correlation coefficients of similarity for the ISSR used are statistically important. The Principal Coordinate Analysis (PCA) for ISSR data also supports its UPGMA clustering. The average number of genetic variations recorded in mulberry genotypes was 0.287±0.096. Dendrogram (Unweighted peer group method analysis) classifies mulberry accessions into two main groups; Admissions collected from the western area of Taif, and the other comprised two sub-clusters including one isolate, i.e., M nigra, a collection from Al shafa. Contains access to another sub-cluster southwest regions of Taif, which belong to Morus nigra wild growing. These accessions of mulberry were found to be genetically similar to north and southwest Taif Province. These results have significant implications for improving the mulberry germ plasma characterization, conservation and investigates of the genetic diversity among the mulberry species grown in Taif governorate and to establish a micro-propagation system as germplasm conservation to preserve the assets of local mulberry and thus develops an easy and effective method to identify native genotypes in a limited space and time frame.

Review on upregulation of glucokinase expression by selected plants and their phytoconstituents

Glucokinase is, an allosteric enzyme involved in glycolysis, is rapidly regulated in the liver through glucokinase regulatory protein and remains essential for blood glucose homeostasis. The present review provides brief information about the effects of a few selected plants and phytochemicals on human glucokinase activation and gene modulation. A thorough and relevant literature search from several scientific databases, comprising Google Scholar, Web of Science, Scopus and PubMed, was carried out. We highlighted the seven plants (Acorus tatariowii Schott, Allium hirtifolium Boiss, Apache red maize, Mulberry species, Pterocarpus marsupium, Sapium ellepticum, Mangifera indica) and their phytoconstituents ( Tatanans A-C, alliogenin , gitogenin , kaempferol , quercetin and shallomin,1-deoxynojirimycin,cyanidin-3-rutinoside,resveratrol,cyanidin-3-glucoside,oxyresveratrol, lupeol, alpha-tocopherol, uteolin-7-glucoside, amentoflavone, and Mangiferin) on human glucokinase enzyme activation and gene modulation. This review concluded that investigation of glucokinase activators of plant origin is the major research focus in the management of type 2 diabetes.