In endurance sports, cycles of high training volume are implemented in order to optimize aerobic and movement economy adaptations. This leads to transient imbalances between training loads and recovery contributing to the onset of fatigue and sometimes illness in highly trained athletes. The well-established interdependence between substrate depletion, hormonal and immune functions elicits usually a immunodepression state as response to hard training periods. PURPOSE: To evaluate the effects of two subsequent training cycles of different load quantitative and intensity characteristics on the response of systemic and mucosal immunity to a maximal swimming bout. METHODS: 6 well-trained male swimmers (18±2.2 yrs; 177±6.3 cm; 67±5.3 Kg) performed an incremental maximal step test (7×200 m front crawl) in 3 moments of the season: M1 - after a recovery microcycle, M2 - after a 5 week period of aerobic overload (volume increased by 20%) and M3 - after 8 weeks of progressively decreasing volume and higher intensities. Blood and saliva samples were collected before (6:30 a.m.) and 5 min after, for the determination of leukocyte and total lymphocyte and subpopulations (CD3+, CD4+, CD8+, CD19+ and CD16+) counts by flow cytometry; serum immunoglobulin A (IgA) concentration by cytometric bead array and salivary IgA (s-IgA) concentrations by ELISA. s-IgA secretory rate (sr-IgA) was calculated from s-IgA values. ANOVA for repeated measures was used for the assessment of training effect. The level of significance was set at p<.05. RESULTS: At rest, CD16+ (NK) cells and IgA decreased significantly from M1 to M2, recovering to initial levels at M3. The acute response (% change) of total lymphocytes, CD3+, CD4+ and CD16+ remained stable from M1 to M2 but was elevated at M3, contrarily to the CD4+/CD8+ ratio, which decreased significantly in M3. CONCLUSIONS: In spite of some signs of impaired immune capacity after the hard training period in what concerns the rest values, values after maximal exercise, even of CD16+ cells, showed high systemic responsiveness. Training load volume seems to be the main determinant of immune changes, with overall recovery of initial levels at M3. During this training cycle it was observed a stimulation of systemic immunity and the maintenance of mucosal immunity.
Read full abstract