Moss Biomonitoring Research Articles

Moss Technique Used to Monitor the Atmospheric Deposition of Trace Elements on the Territory of the Ryazan Region, Russia

Instrumental neutron activation analysis in combination with the method of moss biomonitoring for the assessment of atmospheric deposition of heavy metals and other elements was applied for the first time in the Ryazan Region in Russia. Concentrations of 42 macro, micro and trace elements in 63 moss samples (Hylocomium splendens and Pleurozium schreberi, as well as Hypnum cupressiforme, Sciuro-hypnum sp., Plagiothecium sp. and Ptilium sp.), collected on a relatively uniform grid in the study area, were determined. On the basis of analytical results using GIS technologies, maps of the spatial distribution of heavy metals and other toxic elements were constructed for the Ryazan region. The method of multivariate statistical analysis (factor analysis) was used to identify the main sources of pollution—large industrial facilities located in this region. The obtained results were included in the Atlas of Atmospheric Deposition of Heavy Metals, which is published by the UN Commission on Long-Range Transboundary Air Pollution (UNECE ICP Vegetation).

Trends in Atmospheric Nitrogen Deposition in Macedonia Studied by Using the Moss Biomonitoring Technique

This study examined the nitrogen content in moss samples collected across Macedonia over a 15-year period (2005, 2010, 2015 and 2020) from 72 consistent sampling locations. The nitrogen content was determined using the Kjeldahl method, providing insight into the trends of atmospheric nitrogen deposition across different regions. Descriptive statistical analyses, including spatial distribution maps, were used to compare the temporal variations and regional nitrogen levels. In addition, box-plots (P25–P75) and whiskers (P5–P95) were constructed to provide a comprehensive view of the variability across different tectonic units and zones, allowing for an in-depth understanding of the spatial distribution of nitrogen across the country. The study revealed that the median nitrogen content in moss samples decreased from 1.21% in 2005 to 1.04% in 2015, followed by a slight increase to 1.07% in 2020. The highest nitrogen concentrations were consistently found in areas with heavy agricultural activities and high traffic volumes, indicating the direct impact of these anthropogenic factors. The comparisons across regions and geological zones also highlighted the substantial variation in nitrogen levels, reflecting the diverse environmental pressures in different parts of Macedonia. This long-term analysis not only offers valuable insights into the trends in nitrogen pollution but also underlines the necessity for targeted policy interventions, particularly in the regions where nitrogen levels remain persistently high.

Moss biomonitoring in areas affected by ashfalls of Shiveluch volcano (Kamchatka).

The moss bag technique was applied on Kamchatka to assess the effect of Shiveluch volcano on the air quality. Moss bags were exposed in settlements located at different distances from the volcano and affected by ashfall to varying degrees. The inductively coupled plasma optical emission spectrometry was used for the determination of the content of Al, Ba, Co, Cd, Cr, Cu, Fe, Mn, P, Pb, Sr, S, V, and Zn in both exposed and unexposed moss samples. Relative accumulation factor revealed enrichment of mosses exposed in the area affected by ashfall with Al, Cr, V, and Zn. Correlation and principal component analysis allowed to identify elements of volcanogenic, geogenic, and anthropogenic origin. Ecological risk values less than 150 indicate low potential ecological risk in the region. Active moss biomonitoring can be considered a cheap and efficient tool for in-depth study of the influence of volcanic activity on air quality on the peninsula.

Indoor moss biomonitoring proving construction-related pollution load from outdoors

Indoor pollution load in an office environment was investigated during ongoing construction works in the vicinity using the wet moss biomonitoring technique. Monitoring boxes were placed inside eight offices with mechanical ventilation located on three floors and with four different orientations to outdoor construction works for 8 weeks. Samples were collected every two weeks for two months, and the concentrations of selected elements: Al, As, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Pb, Si and Zn were determined. Total deposition (composition, number and size of particles) was determined in the samples too. Correlation analysis complemented by Principle Component Analysis (PCA) was performed using a compositional approach. Concentrations correlated significantly among crustal elements and among road dust elements. PCA identified three groups of elements – representing construction dust, traffic origin and indoor origin, while those associated with construction dust had the highest contribution. The elevation (floor) affected the composition of the samples the most, the orientation to the pollution sources was secondary. Construction dust was most dominant on the 1st floor; samples from the sheltered sites were negatively associated with construction dust elements; here, the finest particles were also observed. Starting at 6 weeks of exposure, construction dust elements started to dominate in samples. Construction dust is a serious pollution problem, and its infiltration from the outdoors is apparent even in mechanical-ventilated indoor environments. Moss indoor monitoring technique was proven to be a suitable cost-effective alternative to automatic air samplers in determining the outdoor-originating pollution loads indoors.

Moss Biomonitoring in the Evaluation of Air Pollution in the Tver Region, Russia

Moss Biomonitoring in the Evaluation of Air Pollution in the Tver Region, Russia

One year of active moss biomonitoring in the identification of PAHs in an urbanized area—prospects and implications

Classical monitoring of air pollution provides information on environmental quality but involves high costs. An alternative to this method is the use of bioindicators. The purpose of our work was to evaluate atmospheric aerosol pollution by selected polycyclic aromatic hydrocarbons conducted as part of annual active biomonitoring (“moss-bag” technique) with the use of three moss species: Pleurozium schreberi, Sphagnum fallax, and Dicranum polysetum. The gas chromatography-mass spectrometry (GC–MS) was utilized to determine certain 13 polycyclic aromatic hydrocarbons (PAHs). Three seasonal variations in PAH concentrations have been observed as a result of the study. A fire on the toilet paper plant caused an increase of five new compounds: benzo(k)fluoranthene (BkF), benzo(a)pyrene (BaP), indeno(1.2.3)-cd_pyrene (IP), dibenzo(a.h)anthracene (Dah), and benzo(g.h.i)perylene (Bghi) in proximity after 8 months of exposure compared to previous months. The effect of meteorological conditions on the deposition of PAHs (mainly wind direction) in mosses was confirmed by principal component analysis (PCA). Dicranum polysetum moss accumulated on average 26.5% more PAHs than the other species, which allows considering its broader use in active biomonitoring. The “moss-bag” technique demonstrates its feasibility in assessing the source of PAH air pollution in a long-term study. It is recommended to use this biological method as a valuable tool in air quality monitoring.

Moss as a passive biomonitoring tool for the atmospheric deposition and spatial distribution pattern of toxic metals in an industrial city.

Anthropogenic pollution impacts human and environmental health, climate change, and air quality. Karabük, an industrial area from the Black Sea Region in northern Türkiye, is vulnerable to environmental pollution, particularly soil and air. In this research on methodological aspects, we analyzed the concentrations of six potential toxic metals in the atmospheric deposition of the city using the passive method of moss biomonitoring. The ground-growing terrestrial moss, Hypnum cupressiforme Hedw., was collected during the dry season of August 2023 at 20 urban points. The concentrations of Cr, Cu, Cd, Ni, Pb, and Co were determined in mosses by the ICP-MS method. Descriptive statistical analysis was employed to evaluate the status and variance in the spatial distribution of the studied metals, and multivariate analysis, Pearson correlation, and cluster analysis were used to investigate the associations of elements and discuss the most probable sources of these elements in the study area. Cd and Co showed positive and significant inter-element correlations (r > 0.938), representing an anthropogenic association mostly present in the air particles emitted from several metal plants. The results showed substantial impacts from local industry, manufactured activity, and soil dust emissions. Steel and iron smelter plants and cement factories are the biggest emitters of trace metals in the Karabük area and the primary sources of Cr, Cd, Ni, and Co deposition.

Mosses as bioindicators of air pollution with potentially toxic elements in the Burabay State National Natural Park, Kazakhstan.

The Burabay State National Natural Park is a national park of the great natural and historical values located in the north of Kazakhstan, which has been exposed in recent years to significant anthropogenic impact. The moss biomonitoring was performed in the Borovoye resort community, an important tourist destination in the national park, to identify the level of air pollution. Mosses collected at 29 locations were subjected to neutron activation analysis to determine 36 elements and additionally to ICP-OES to detect the level of Cu and Pb. Factor analysis was applied to check if there are any associations between identified elements and to link them with possible emission sources. According to contamination factor and pollution load indices the investigated area belongs to three classes of pollution: unpolluted, suspected and moderate. Potential ecological risk index calculated for selected elements revealed harmless risk to human health. The level of element obtained in Burabay State National Natural Park was compared with the data available for other national parks.

Moss biomonitoring of air quality linked with trace metals pollution around a metallurgical complex in Elbasan, Albania

Moss biomonitoring of air quality linked with trace metals pollution around a metallurgical complex in Elbasan, Albania

Using Factor Analysis To Find Source Atmospheric Pollution By Moss Technique

The aim of this investigation was to apply the moss biomonitoring technique using Barbula Indica moss and factor analysis to evaluate the possible sources of atmospheric pollution in the Thua Thien Hue province. The Barbula Indica moss samples were collected at sixteen sites in the areas of Thua Thien Hue province, the central Vietnam. The concentrations of thirty elements in the collected Barbula Indica moss samples were determined by neutron activation analyses at the reactor IBR-2 at the Joint Institute for Nuclear Research (JINR) in Dubna, Russian Federation. Factor analysis has been applied to the obtained concentrations to reveal the possible pollution sources. Rotated factor loadings suggested three factors, that could explained more than 84% of variability. Additionally, factor scores were calculated, that confirmed the presence of pollution sources in the corresponding sites.

Assessment of Air Pollution in Ulaanbaatar Using the Moss Bag Technique.

Active moss biomonitoring, the so-called moss bag technique, widely applied in many countries, for the first time, was applied to assess the air quality in Ulaanbaatar (Mongolia). Moss bags with Sphagnum girgensohnii Russow were exposed in triplicate in three different periods: December-February, March-May, and December-May at 13 governmental air quality monitoring stations located in the vicinity of thermal power plants and residential areas. The plant tissue content of Al, Ba, Co, Cd, Cr, Cu, Fe, Mn, P, Pb, Sr, S, V, As, and Zn was determined using inductively coupled plasma-optical emission spectrometry, and a direct mercury analyzer was used to determine the Hg content. The samples in residential areas and near thermal power plants that were exposed for 3months in winter and for 6months (winter to spring) were characterized by the highest accumulation of the elements. In the moss bags exposed during spring, maximum accumulation of the determined elements was noted in residential areas and near main roads. Regardless of the exposure time and duration, the highest accumulation of Al, Fe, and V was determined at Dambadarjaa air quality station located near a highway and of Hg near the Amgalan power plant. Significant differences in element accumulation between seasons were observed, thus, the accumulation of Al, Ba, As, Co, Cr, Fe, Pb, V, and Zn was higher in spring, while P and S had higher content in the moss samples exposed during winter. The accumulation of elements over the 6-month exposure period was 1.1-6.7 times higher than that of the 3-month periods. Thus, the 6-month exposure can be considered a reliable deployment period as it ensures an adequate signal in terms of enrichment of pollutants. Factor analysis was applied to highlight the association of elements and to link them with possible sources of emission. Three factors were determined, the first one included Al, As, Ba, Co, Cr, Fe, Mn, Pb, Sr, and V and was identified as a geogenic-anthropogenic, the second (Cu, P, and S) and third (Cd and Zn) factors suggested anthropogenic origin. The Relative accumulation factor and enrichment factor were calculated to evaluate the level of air pollution and possible element sources. Considerable contributors to air pollution were Zn, Fe, As, V, Cr, and Al, which may originate from airborne soil particles of crustal matter or transport, as well as coal combustion for heating and cooking.

Assessment of Atmospheric Deposition of Potentially Toxic Elements in Macedonia Using a Moss Biomonitoring Technique

This study aims to investigate the changes in atmospheric deposition trends in Macedonia, using a moss biomonitoring technique. This technique has been used to assess the content of potentially toxic elements in Macedonia in 2002, 2005, 2010, and 2015, within the framework of the International Cooperative Program on Effects of Air Pollution on Natural Vegetation and Crops. The content of 42 elements was analyzed using instrumental neutron activation analysis (INAA), inductively coupled plasma–atomic emission spectrometry (ICP–AES), and atomic absorption spectrometry (AAS), on 72 moss samples collected in the summer of 2015. The median values of the elements studied were compared with data from previous years and with median values obtained from comparable studies in Norway and the neighboring countries. Through factor and cluster analysis, three geogenic factors were identified: Factor 1, which includes the elements Al, Ce, Fe, Hf, La, Li, Na, Sc, Sm, Tb, Ti, Th, V, and U; Factor 4, which includes As, Cl, and I; and Factor 5, which includes the elements Ba and Sr. In addition, one geogenic-anthropogenic factor containing Co, Cr, and Ni (Factor 2), was identified, and one anthropogenic factor containing Cd, Pb, Sb, and Zn (Factor 3). The lead and zinc mines near the towns of Kriva Palanka, Probištip, and Makedonska Kamenica in the eastern region of the country, the former lead and zinc smelter in the town of Veles, and the ferronickel smelter near Kavadarci, have continuously had the greatest anthropogenic impact on the atmospheric deposition of potentially toxic elements during the time period of the study. In addition to the human influences, the lithology and the composition of the soil continue to play a significant role in the distribution of the elements.

15 Years of Moss Biomonitoring for Air Quality Assessment in Albania

15 Years of Moss Biomonitoring for Air Quality Assessment in Albania

Moss biomonitoring of the distribution of chemical elements in the air in the Prespa region, North Macedonia

Moss biomonitoring of the distribution of chemical elements in the air in the Prespa region, North Macedonia

Active moss biomonitoring of airborne potentially toxic elements in recreational areas of Moscow.

Active biomonitoring using the moss bag technique was applied to examine the atmospheric deposition of potentially toxic and other elements in recreational areas of Moscow. Moss bags with Sphagnum girgensohnii were placed in the territory of seven parks (Losiny Ostrov, Victory Park, Ostankino, Sokolniki, Izmailovo, Kuzminki-Lyublino, Tsaritsyno) at three locations in each park from June to September 2018. The content of 32 chemical elements: Na, Mg, Al, Cl, K, Ca, Sc, V, Mn, Fe, Co, Zn, As, Br, Rb, Sr, Mo, Sb, Cs, Ba, La, Sm, Tb, Hf, Ta, W, Au, Th, U, Cu, Pb, and Cd in moss samples was determined by instrumental neutron activation analysis and atomic absorption spectrometry. After a 3-month exposure period, high uptake of Sb, U, Th, Sm, La, Mo, Zn, Co, Fe, V, Sc, etc. was observed in some of the moss samples. The physiologically active elements Cl and K and alkaline elements Rb and Cs were depleted from the moss tissue during the exposure. The high accumulation of Zn, Pb, Cu, Co, V, and Sb in moss samples evidenced an anthropogenic impact on the parks, mainly associated with road traffic. To determine the level of pollution, a set of environmental indices was calculated: contamination factor (CF), enrichment factor (EF), total pollution index (TPI), and relative accumulation factor (RAF). The highest RAF values were obtained for Sb on the territory of all parks. According to EF, the samples were enriched in Al, Fe, U, Pb, Cd, Au, Sb, Th, and Ta. High CF values were obtained for sites in Losiny Ostrov, Izmailovo, Tsaritsyno, and Kuzminki-Lyublino located close to roads. According to TPI, the level of air pollution on the territory of the abovementioned parks varied from moderate to high. To identify the major sources of pollution, correlation analysis and principal component analysis were applied.

Assessment of the air quality in an industrial zone using active moss biomonitoring

The study aimed to evaluate the level of air contamination in the area around the Kosogorsky industrial enterprise in Tula, Russia, in the winter of 2021/2022. For the study, Pleurozium schreberi, Sphagnum fallax, and Dicranum polysetum mosses were used for the first time in the 3-month active biomonitoring (moss-bag technique). Heavy metals elements (Mn, Fe, Ni, Cu, Zn, Cd, and Pb) were determined using atomic absorption spectrometry. In addition, mercury concentrations were determined with an AMA254 apparatus. The study's results for Fe, Cu, Zn, Pb, and Hg confirm the statistical significance of the species' effect on the accumulation of these elements. Values of relative accumulation factor (RAF) > 1.00 at selected measurement points indicate that the site is contaminated as a result of industrial activities (mainly Mn and Fe). Wind direction influenced moss contamination at selected measurement points, which was confirmed by cluster analysis. In the future, long-term or different seasons biomonitoring studies should be conducted in this area.

Study on moss biomonitoring of atmospheric deposition of minor and trace elements in the region of Polog, North Macedonia

AbstractA study was conducted to investigate air deposition and to explore the natural or anthropogenic sources of contamination with potentially toxic elements (PTEs) in the region of Polog, North Macedonia, using moss samples as biomonitors of air pollution. The distribution of 22 elements was detected in 43 moss samples collected in the region. Multivariate statistical analysis was applied to the available data. Factor analysis identified five association factors, of which four factors, F1 (Cr, Li, V, Co, Fe, Al, Ni, and As), F2 (K, P, and Mg), F4 (Ag and Cu), and F5 (Ba, Sr, and Mn) were related to lithogenic sources and one factor F3 (Hg, Pb, Cd, and Zn) was related to mixed lithogenic and anthropogenic sources. The content of the elements of F1 is higher in the areas on Mount Šar (area without industrial and urban activities), where Precambrian and Paleozoic shales and Paleozoic sandstones predominate, indicating that their origin is mainly a natural phenomenon. The high contents of the elements from F2, F4, and F5 are also related to the lithology of the studied region. Thus, the high contents of elements from F2 are distributed in the western part of the area, where Precambrian and Paleozoic shists and Paleozoic sandstones dominate, and their contents correlate well with the contents of lithogenic elements in the soil samples from the same region. Elements of Factor 4 have higher contents in the southern part of the region, in areas with Paleozoic‐Mesozoic carbonates and Precambrian‐Paleozoic shales, while elements of Factor 5 occur in the regions with shales, Quaternary sedimentary rocks, and Precambrian‐Paleozoic shales, also indicating the lithogenic character of this factor. In addition to the lithogenic origin of the distribution of elements contained in F3 (Hg, Pb, Cd, and Zn), anthropogenic activities also have an influence on the increased content in the northeastern part of the region. This is due to dust emissions from the Silmak ferrosilicon smelter, as well as emissions from industry (steel smelters) and urban activities of the nearby capital Skopje.

Approach to Evaluate the Data of Moss Biomonitoring Studies: Preprocessing and Preliminary Ranking

This paper, dedicated to Acad. Florin Gheorghe Filip, at his 15th anniversary as the Chairman of the Information Science and Technology Section of the Romanian Academy, suggests an approach to describe the data obtained in biomonitoring studies using mosses, on the example of the Republic of Moldova. In total, 33 moss samples were collected on the territory of Moldova, the elemental composition of which was determined by neutron activation analysis and atomic absorption spectrometry. At the first stage of the work, a correlation analysis was carried out with the ranking of data in the order of decreasing total correlation, which made it possible to preliminarily reduce the number of elements to two factors. At the stage of data sorting, iron, the content of which in the environment can be associated with anthropogenic activity, was chosen as the element determining the rank. The next stage of work was data smoothing using a discrete cosine transform, for which the codes were rewritten and the algorithm was ported to the Excel-VBA environment, which is most suitable for preprocessing and graphical display of experimental data. Also, an algorithm was developed for determining the number of linearly independent (basis) vectors in which the matrix itself can be decomposed. Two principal roots were identified, of which the larger one is several thousand units, and three lower roots, which are less than unit were excluded, since their absolute values differ by two to three or more orders of magnitude downward from the eigenvalues of the influencing components. The paper is dedicated to Acad. Florin Gheorghe Filip, at his 15th anniversary as the Chairman of the Information Science and Technology Section of the Romanian Academy, and at his 75th anniversary.

Moss-bag technique as an approach to monitor elemental concentration indoors

The moss-bag technique has been used for many decades to monitor outdoor pollution. More recently, however, the method has been used to monitor indoor air pollution (IAP), as humans spend the majority of their time indoors. The purpose of the research conducted was to evaluate indoor air pollution using active moss biomonitoring. Pleurozium schreberi moss bags were exposed for two seasons (summer and winter), hanging over tile stoves and coal stoves. The selected elements: Al, Cu, Cd, Co, Pb, Zn, V, Ba, Cr, Fe, Mn, Sr, P, Ni, and S were determined by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES) and, for Hg, by a direct mercury analyzer. The study found the exposure season affected the concentrations of selected elements in 62.5% of cases, and their source was identified. The average concentrations of Co, Ba, Cr, and Sr were higher, and statistically significant, in winter, after a 12-week exposure period of the mosses, regardless of the type of heating or cooking stove owned. The higher phosphorus concentrations obtained in summer indicate physiological stress caused by unfavorable winter exposure conditions. In the future, the number of species used to assess indoor air pollution should be increased and the range of pollutants expanded, along with the identification of their sources, taking residents’ lifestyles into account.

Investigation of Elemental Deposition in Lamdong Province (Vietnam) by the Moss Biomonitoring Method and Neutron Activation Analysis

The results of an investigation of heavy metal air pollution in Lamdong province (Vietnam) using the moss biomonitoring method are presented in this paper. Barbula Indica moss samples were collected at 30 different locations in Dalat and Baoloc, two major cities of Lamdong province. The concentrations of 10 heavy metal and metalloid elements, Sc, V, Cr, Mn, Fe, Co, Zn, As, Hf, and Ta in the collected moss samples, were determined by the neutron activation analysis method using the nuclear reactor at the Nuclear Research Institute in Dalat. The results show that the air in Lamdong province may be polluted by Mn and is moderately polluted by Sc, V, Cr, Fe, Co, Zn, As, Hf and Ta. The main sources of heavy metal pollution in the Lamdong atmosphere may be soil dust, traffic emissions, industry, bauxite mining and refining, chemical fertilizers and pesticides, and combustion of coal and oil. By comparing heavy element concentrations in the moss from Lamdong with that of Hanoi, Thainguyen, and several European countries, it is found that heavy metal air pollution in Lamdong is much lower than in Hanoi and Thainguyen and much higher than in the European countries.