This study investigates the impact of Bacillus subtilis and PET fiber additions on the mechanical properties of alkali-activated composite mortars (AACMs). Blast furnace slag (BFS) is used as the primary binder, activated by sodium hydroxide (NaOH) and sodium silicate (Na2SiO3). Controlled curing conditions at 20 °C and 100 % relative humidity for 28 days were maintained. Comprehensive tests, including compressive strength, flexural strength, and scanning electron microscopy (SEM) analyses, were conducted. Results showed that AACMs incorporating Bacillus subtilis exhibited a 25 % increase in compressive strength and a 20 % increase in flexural strength compared to control samples. The addition of PET fibers further improved these properties by 10 % and 8 %, respectively. These findings highlight the potential of bio-enhanced alkali-activated materials in sustainable construction.