Moringa Oleifera Seed Extract Research Articles

Enhancing the coagulation process for the removal of microplastics from water by anionic polyacrylamide and natural-based Moringaoleifera

The existence of microplastics (MPs) in water is a significant global concern since they have the potential to pose a threat to human health. Therefore, there is a need to develop a sustainable treatment technology for MPs removal, as the conventional methods are inadequate to address this problem. Coagulation is a typical process in treatment plants that can capture MPs before releasing them into the environment. In this work, the removal behaviors of polyamide (PA), polystyrene (PS), and polyethylene (PE) MPs were systematically investigated through coagulation processes using aluminum sulfate (Al2(SO4)3) and Moringa oleifera (MO) seeds extract. Subsequently, the coagulation performance of Al2(SO4)3 was improved by the separate addition of anionic polyacrylamide (APAM) and naturally derived MO. Results showed that Al2(SO4)3 in combination with APAM had better performance than Al2(SO4)3 or MO alone. In the Al2(SO4)3+APAM system, the removal efficiencies were 93.47%, 81.25%, and 29.48% for PA, PS, and PE MPs, respectively. Furthermore, the effectiveness of the Al2(SO4)3 and MO blended system was approximately similar to the Al2(SO4)3+APAM system. However, the required amount of Al2(SO4)3 was decreased to 50% in the Al2(SO4)3+MO system compared to the optimal dosage in the Al2(SO4)3 system alone. The combination of 40 mg/L of Al2(SO4)3 and 60 mg/L of MO resulted in removal efficiencies of 92.99%, 80.48%, and 28.94% for PA, PS, and PE MPs, respectively. The high efficacy of these enhanced methods was due to the synergic effects of charge neutralization and agglomeration adsorption, which were validated through zeta potential assessments and visual analysis using scanning electron microscopy (SEM) images. In the case of experimental conditions, initial pH had little impact on removal efficiency, while NaCl salinity and stirring speed directly affected MPs removal. Consequently, this research took a step toward finding a green strategy to remove MPs from water systems.

Statistical evaluation of the coagulation-flocculation process by using Moringa oleifera seeds extract to reduce dairy industry wastewater turbidity

The use of coagulant aqueous extract obtained from M. oleifera seeds in the integration of coagulation/flocculation processes and membrane separation proved to be an environmentally friendly alternative for wastewater treatment from the dairy industry. Three saline solutions, KCl, MgCl2 and CaCl2, were tested to extract the active component from Moringa seeds. The greatest statistical effect on turbidity removal was attributed to the initial pH of the effluent, according to the t-Student at the 5 % significance level. The maximized turbidity and color removals were 96.8 and 90.3 %, respectively, at pH 11 and 300 mg L−1 of the coagulant extracted with CaCl2 0.9 mol L−1. Nanofiltration indicated that previously flocculated organic matter particles were reversibly retained by the membrane. Total fouling was only 35 %, resulting in 98 % recovery of the initial water flow, and a reduction in color, turbidity, COD, calcium, and hardness of 99.5; 98.9; 89.1; 85.5 and 85 %, respectively.

The Potential of Moringa oleifera Seed in Water Coagulation-Flocculation Technique to Reduce Water Turbidity

Using a safe and easy-to-apply economic flocculant to replace alum is necessary to expand and enhance the water quality in rural regions where traditional water treatment is unavailable. The seed of Moringa oleifera is locally available in large volumes and is feasibly economical. A compatibility and applicability research of the easily accessible local Moringa oleifera seed extract (MOSE) was conducted to examine its potential utilization to lessen various degrees of water turbidity at 30 ± 2 °C. The study concerns the optimum dosage of MOSE to give high turbidity removal efficiency in the water. Experiments were carried out for nine turbidity samples: 5, 10, 15, 30, 50, 70, 100, 200, and 300 NTU. The turbidity removal efficiency of up to 89% was achieved for high initial turbid water 300 NTU. However, for low turbid water, its potential efficiency declined. The results demonstrate that the de-oiled extract is substantially more efficient than the crude extract in agglomerating colloidal particles of low initial turbidity samples and achieved up to 76% removal rate. The results of the ANOVA confirmed that the regression model was significant at (p < 0.05) for residual turbidity after the treatment process. The study also demonstrates that a pseudo-first-order kinetic model matched well the nature of the removal of colloidal particles with MOSE in water to form flocs. The study also indicates that the characteristics of water treated with crude MOSE produce little change in electrical conductivity and salinity. However, the hardness and alkalinity of water decreased significantly depending on the initial hardness and the amount of MOSE applied (p < 0.05).

Antimicrobial Activity of Moringa oleifera Tea Leaves and Seeds Concentrated in Di Ethanol against E. coli Isolated from Ostrich Feces

Ostriches are frequently infected with viral, fungal, and bacterial diseases. This disease does not require airborne transmission and does not involve the respiratory system. Objective: To determine the antimicrobial activity of Moringa oleifera tea leaves and seed against bacteria in Ostrich feces. Methods: Fecal samples were collected from captive ostriches at the W.A Apparel factory. E. coli was isolated after the samples were inoculated on EMB. The antimicrobial activity of Moringa oleifera seeds and tea leaves was investigated. The antimicrobial activity of Ostrich feces was tested against E. coli. Results: The results showed that tea extract had no antimicrobial activity against E. coli. Moringa oleifera seeds extract prepared in ethanol on the other hand, were effective against E. coli. Conclusion: Moringa oleifera seeds (di ethanolic extract) have the potential to be effective against E. coli.

Evaluation of the Impacts of Potassium Bicarbonate, Moringa oleifera Seed Extract, and Bacillus subtilis on Sugar Beet Powdery Mildew.

Powdery mildew disease, caused by Erysiphe betae, is one of the most threatening diseases on sugar beet plants worldwide. It causes a great loss in the root yield, sugar percentage, and quality of produced sugar. In the current study, we aimed to evaluate the susceptibility of 25 sugar beet cultivars to infection with powdery mildew disease under Egyptian conditions. Moreover, we evaluated the impacts of three eco-friendly materials, including potassium bicarbonate (KHCO3; at 5 and 10 g L-1), Moringa oleifera seed extract (25 and 50 g L-1), and the biocontrol agent, Bacillus subtilis (108 cell suspension) against E. betae in two successive seasons 2020 and 2021. Our findings showed that there were significant differences between these 25 cultivars in their susceptibility to the disease under study. Using the detached leaves technique in vitro, B. subtilis showed strong antifungal activity against E. betae. Moreover, both concentrations of KHCO3 and moringa seed extract significantly reduced the disease severity. Under field conditions, tested treatments significantly reduced the severity of powdery mildew disease and prevented E. betae from producing its conidiophores and conidia. Scanning electron microscope examination of treated leaves demonstrated the presence of the decomposition of fungal hyphae, conidiophores, conidia, and the occurrence of plasmolysis to fungal cells and spores on the surface of the leaves. Furthermore, these treatments greatly improved the percent of sucrose and soluble solids content, as well as the enzymatic activity of peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. It is noteworthy that treatment with moringa seed extract gave the best results, followed by potassium bicarbonate, then B. subtilis cell suspension. Generally, it is recommended to use the substances used in this research to combat powdery mildew to minimize or prevent the use of chemical fungicides harmful to public health and the environment.

Study on the Hypolipidemic Effect of Moringa oleifera Seeds Based on Zebrafish Hyperlipidemia Model

Objective: Investigated the hypolipidemic effect of alcoholic extract of Moringa oleifera seeds using the zebrafish hyperlipidemia model.&#x0D; Methods: Using 0.3% egg yolk solution to feed zebrafish larval to construct the hyperlipidemia model. These larval fish in the model were treated with an extract of Moringa oleifera seeds for 2 days. Then, the tail vessels were observed after oil red O staining, and the accumulated optical density of lipids was analyzed. The body length, triglyceride (TG), and cholesterol (TC) levels were measured. Result: The accumulated optical density of lipids in the tail vessels of larval fish fed with 0.3% egg yolk was significantly higher than that of the blank group. The contents of TG and TC were also increased, indicating that the hyperlipidemia model was successfully established. After treatment with different concentrations of Moringa oleifera seed extract (10 μg/ml, 15 μg/ml, and 20 μg/ml), the staining attachment surface of vascular oil red O in the tail of hyperlipidemic zebrafish was significantly reduced by 42.8%, 63.3%, and 80.6% respectively. TG was reduced by 9.89%, 20.41%, and 26.40%. TC was reduced by 9.85%, 23.93%, and 33.80%. The data of each group were statistically significant (P&lt;0.01).&#x0D; Conclusion: Moringa seeds had a significant hypolipidemic effect on zebrafish and had no significant effect on the skeletal growth length of zebrafish.

Effect of Moringa oleifera Seed Extract Administered through Drinking Water on Physiological Responses, Carcass and Meat Quality Traits, and Bone Parameters in Broiler Chickens

The efficacy of Moringa oleifera seed extract (MSE) in broiler nutrition is unknown. Thus, this study evaluated the effect of administering MSE through drinking water on physiological responses, carcass and meat quality traits, and bone parameters in Cobb 500 broilers. One-day-old male chicks (n = 250, 58.1 ± 0.23 g live-weight) were randomly allotted into five treatments of five replicates. The treatments were: negative control with drinking water only (NC); a positive control with 5 g probiotic (containing Aspergillus oryzae, Bacillus subtilis, Enterococcus faecium, and Lactobacillus acidophilus; bacterial count of 5 × 108 CFU/g) added into 1 L of drinking water (PC), 60 mL MSE/L drinking water (MSE60); 90 mL MSE/L drinking water (MSE90); and 120 mL MSE/L drinking water (MSE120). The MSE groups promoted higher (p &lt; 0.05) carcass weights than the PC and NC control groups. Spleen weights quadratically responded to MSE levels. Gizzard weight, duodenum length, acidic goblet cell count (GCC), and total duodenal and ileal GCC linearly increased with MSE levels. Tibia weight, bone breaking strength, and tibia calcium showed linear increases whereas tibia length and tibiotarsal index showed linear decreases with MSE levels. It can be concluded that the administration of MSE via drinking water improved carcass weights, intestinal morphology, and some meat and bone quality parameters of the birds.

Moringa oleifera seeds extract improves the quality of treated wastewater of Byad-Elarab plant, Beni-Suef, Egypt

Moringa oleifera seeds extract improves the quality of treated wastewater of Byad-Elarab plant, Beni-Suef, Egypt

Mechanistic Wound Healing and Antioxidant Potential of Moringa oleifera Seeds Extract Supported by Metabolic Profiling, In Silico Network Design, Molecular Docking, and In Vivo Studies.

Moringa oleifera Lam. (Moringaceae) is an adaptable plant with promising phytoconstituents, interesting medicinal uses, and nutritional importance. Chemical profiling of M. oleifera seeds assisted by LC-HRMS (HPLC system coupled to a high resolution mass detector) led to the dereplication of 19 metabolites. Additionally, the wound healing potential of M. oleifera seed extract was investigated in male New Zealand Dutch strain albino rabbits and supported by histopathological examinations. Moreover, the molecular mechanisms were investigated via different in vitro investigations and through analyzing the relative gene and protein expression patterns. When compared to the untreated and MEBO®-treated groups, topical administration of M. oleifera extract on excision wounds resulted in a substantial increase in wound healing rate (p < 0.001), elevating TGF-β1, VEGF, Type I collagen relative expression, and reducing inflammatory markers such as IL-1β and TNF-α. In vitro antioxidant assays showed that the extract displayed strong scavenging effects to peroxides and superoxide free radicals. In silico studies using a molecular docking approach against TNF-α, TGFBR1, and IL-1β showed that some metabolites in M. oleifera seed extract can bind to the active sites of three wound-healing related proteins. Protein–protein interaction (PPI) and compound–protein interaction (CPI) networks were constructed as well. Quercetin, caffeic acid, and kaempferol showed the highest connectivity with the putative proteins. In silico drug likeness studies revealed that almost all compounds comply with both Lipinski’s and Veber’s rule. According to the previous findings, an in vitro study was carried out on the pure compounds, including quercetin, kaempferol, and caffeic acid (identified from M. oleifera) to validate the proposed approach and to verify their potential effectiveness. Their inhibitory potential was evaluated against the pro-inflammatory cytokine IL-6 and against the endopeptidase MMPs (matrix metalloproteinases) subtype I and II, with highest activity being observed for kaempferol. Hence, M. oleifera seeds could be a promising source of bioactive compounds with potential antioxidant and wound healing capabilities.

Effect of Moringa oleifera seed extract on antimicrobial activity and in vitro fertilization ability of cryopreserved ram semen.

Cryopreservation has adverse effects on the post-thaw sperm quality due to oxidative stress and the presence of bacteria. To minimize such effects, plant extracts have been included in the composition of the semen diluents. The objective of the present study was to evaluate the antimicrobial and antioxidant effects of Moringa oleifera seed extract (MOSE) on cryopreserved ram semen, as well as its impact on in vitro fertilization. Semen from six hair rams was treated with five treatments before cryopreservation: Control (without any antibiotic), Standard (conventional antibiotic), 1.0, 10.0, and 50.0mg/ml of MOSE. Post-thawing sperm characteristics were evaluated by the computer-assisted semen analysis. Antimicrobial activity was assessed by counting colony-forming units (CFU) and the antioxidant capacity by the ferric reducing antioxidant power method. A heterologous in vitro fertilization technique was implemented to measure the fertilization rate. Progressive and rapid motility, membrane and acrosome integrity, and active mitochondria were higher (p < .05) in the 10.0mg/ml treatment compared with Standard after thawing. All M. oleifera treatments showed inhibition of CFU. The antioxidant capacity of M. oleifera seed extract was higher in the 10.0 and 50.0mg/ml treatments. Fertilization rate (cleavage percentage) was higher (p < .05) in the 10.0mg/ml (82.9 ± 10.0) and Control (82.5 ± 9.9) treatments compared with Standard (73.7 ± 9.1). The addition of 10.0mg/ml of MOSE to ram semen inhibits the development of microorganisms and improves sperm characteristics and the in vitro fertility of the semen.

Assessment of moringa oleifera seeds extract effect on the adverse effects of levofloxacin drug induced liver injury

The current study aimed to know the protective role of the cold aqueous extract of Moringa seeds against the toxic damages caused by levofloxin in male albino rats by studying biochemical parameters. 24 male white rats were randomly divided into four groups, comprising (6 animals per group). The first group (G1) as a control group was dosed orally and daily with physiological solution for 30 days, the second group (G2)) was dosed with levofloxacin at a concentration of 10 mg / kg of body weight, the third group (G3) was dosed with aqueous extract of Moringa oleifera seeds at a concentration of 350 mg / kg The fourth group (G4) was dosed with aqueous extract of Moringa oleifera seeds 350 mg/kg 4 hours before the administration of levofloxacin at a concentration of 10 mg/kg body weight. Male rats in all study groups were administered orally via the dosing tube, with one dose per day for 30 days. . Blood samples were collected in the four groups after one month of dosing to measure the level of Malona dialdehyde and glutathione, as well as the levels of liver enzymes aspartate transaminase (AST), alanine transaminase (ALT) and alkalinephosphatase (ALP).

Ileum Histoarchitecture Following Aspartame Consumption: Assessment of Actions of Moringa oleifera Seed Extract

This study aims to determine the effect of Moringa oleifera seed extract on ileum histoarchitecture following aspartame consumption. Twenty-seven adult male Albino Wistar rats were used for the study. They were divided into nine groups comprising of: group A (control), administered distilled water; group B, given 0.4 ml (low dose) aspartame; group C, given 1.5 ml (high dose) aspartame; group D1, given 0.6 ml (low dose) aspartame for the first three weeks followed by 1.5 ml (low dose) ethanolic extract of M. oleifera seed on the fourth week; group D2 given 0.5 ml (low dose) aspartame for the first three weeks followed by 1.30 ml (low dose) n-hexane extract of M. oleifera seed on the fourth week; group E1, given 1.5 ml (high dose) aspartame for the first three weeks and on the fourth week 2.5 ml (high dose) ethanolic extract of M. oleifera seed; group E2, given 1.5(high dose) aspartame for the first three weeks and on the fourth week 3.0 ml (high dose) n-hexane extract of M. oleifera seed; group F1 given 1.4ml (high dose) aspartame followed by 2.8ml (high dose) ethanolic extract of M. oleifera seed for four weeks; group F2, given 1.3 ml (high dose) aspartame followed by 2.5 ml (high dose) n-hexane extract of M. oleifera seed for four weeks. Weight measurement of rats was taken once a week throughout the twenty eight days duration of the study. Following anaesthetization, their ileum were harvested and fixed in 10 % buffered formalin. Hematoxylin and Eosin (H&amp;E) staining technique was adopted for routine histological procedure. Data were expressed as mean ± standard error of mean (M ± SEM) and subjected to one-way analysis of variance (ANOVA). At 95 % confidence interval, P is significant (P&lt; 0.05). There was a significant increase (P= 0.05) in body weight of both the control and the experimental groups as the experiment progressed. Histological findings revealed that aspartame both in low and high doses has disruptive effects on the histoarchitecture of ileum and high dose ethanolic extract of M. oleiferia seed has the capacity to ameliorate the effects. Hence, caution is needed in aspartame consumption.

Evaluating the Efficacy of Moringa oleifera Seed Extract on Nutrient Digestibility and Physiological Parameters of Broiler Chickens

Moringa oleifera seed extract (MSE) contains phytochemicals that can improve chicken production and health. However, the amount that can be orally administered to the birds is unknown. Thus, this study investigated the optimum level of MSE, administered through drinking water, on nutrient digestibility, growth performance, and haematological and serum biochemical parameters in broilers. A total of 250, one-day-old Cobb 500 male broilers (58.11 ± 0.23 g live-weight) were randomly allocated into five treatments with five replicates of 10 birds each. The treatments were: a negative control involving drinking water only (T1) and positive controls with 5 g multi-strain probiotic/L water (T2), 60 mL MSE/L water (T3), 90 mL MSE/L water (T4), and 120 mL MSE/L water (T5). Crude protein and neutral detergent fibre digestibility linearly decreased, whereas overall water intake and weight gain in one-week-old birds increased linearly (p &lt; 0.05) as MSE levels increased. T1 promoted higher (p &lt; 0.05) overall feed intake and feed conversion ratio than the other treatment groups. All haemato-biochemical parameters were within the range for healthy birds, from which a maximum MSE dosage was determined to be 94.75 mL/L. It can be concluded that oral administration of MSE improved overall feed utilisation efficiency of the chickens.

Protective effect of aqueous of Moringa Oleifera seeds extract against levofloxacin drug-induced liver damage in male white rats

Objective The goal of this study is to find out how effective Moringa seed (Moringa oleifera) aqueous Extract is in protecting liver tissue from oxidative stress caused by levofloxacin drug . Methods: 24 male albino rats were placed into four groups at random, with six rats in each group being treated for 30 days: Group 1 was a control group, whereas Group 2 was given levofloxacin at a dosage of 10 mg/kg orally by gavage. , The third group received aqueous extracts of Moringa seeds 650 mg/kg, while the fourth group received aqueous extracts of Moringa seeds 650 mg/kg dose before 4 hours of receive orally at a 10 mg/kg per day dose . Results: The albino rat was considerably protected from levofloxacin-induced liver damage when given the aqueous extract Moringa seeds orally at a concentration of 650 mg/kg body weight. The action of the extract also protected the liver damage caused by the medication, which was indicated by congestion central vein degeneration hepatic cell, necrosis hepatic cell, irregular hepatocyte organization, dilatation and congestion of portal vein , Expandation of sinusoids and increased infiltration of inflammatory cells on histological examination.

Moringa oleifera seed extract assisted electrocoagulation process for efficient direct dye removal from textile wastewater: Modelling, optimisation and techno-economic study

ABSTRACT This work reports the results of coupling Moringa oleifera seed extract (MOSE) with electrocoagulation (EC) for the remediation of Direct Red 80 azo dye (DR80) from contaminated synthetic aqueous solution and real textile wastewater. The electrochemical treatment was carried out using a pair of aluminium electrodes in monopolar-parallel mode. EC runs determined using Box-Behnken design under the response surface methodology (RSM) for three parameters including current density, pH, and operation time. The EC optimal conditions were current density 0.9 mA/cm2, pH 3, and time 10 min. The maximum experimental DR80 dye removal of 89.37% was close to the model prediction (76.4%). The kinetic study of the EC system represented that the removal of dye obeyed the first-order kinetic model. To improve dye removal efficiency from treated wastewater under optimal conditions, 2.5 mL/L of MOSE was added and the efficiency enhanced to above 98%. SEM and microscopic images of the sludge produced showed that the flocs formed in the hybrid system were denser and thicker than the conventional EC. The economic study revealed that switching from the EC to hybrid process reduced energy and electrode consumption from 0.015 to 0.008 kWh/m3 and 0.016 to 0.014 kg/m3, respectively, for 98% DR80 dye removal. MOSE has a substantial effect on the EC performance. Therefore, the EC/MOSE coupling process is recommended for the treatment of coloured wastewater due to its high efficiency, short operation time, and electrode and energy consumption costs.

Optimization of the production of local cheese from cow milk processed with the seed ofMoringa oleifera

This study was carried out to optimize the production process and assess the quality parameters and shelf life of cheese product obtained from cow's milk using Moringa oleifera seeds extract as a coagulant. Optimum processing conditions were determined using response surface methodology (RSM). The optimum conditions established showed 1.5% concentration of M. oleifera seed extract and 55°C coagulation temperature. Cheese made was subjected to physicochemical, sensory, and microbiological analyses and compared with cheese produced using Calotropis procera extract as a coagulant (CPC1). Shelf life studies were carried out by subjecting them to storage at ambient and refrigeration temperatures for 10 days. Results showed that cheese produced using M. oleifera seed extract as a coagulant (MOC) had the highest protein and fat contents of 28.14% and 17.68%, respectively. The sensory attributes were improved and it had the longest shelf life of 8 days at ambient temperature (28 ± 2°C). This study showed that optimization of the cheese production resulted in cheese with better proximate characteristics, sensory attributes, and longer shelf stability. Novelty impact statement This research showed that Moringa oleifera seeds extract has better coagulating and antioxidant properties when compared with the conventional extract (Calotropis procera) used in the production of local cheese. The cheese produced with the former had a better yield, proximate characteristics, sensory attributes, and longer shelf stability. Basically, the preservative ability of this natural extract is very commendable as it does not have any side effects unlike other synthetic preservatives used in dairy products and is also readily available.

Influence of Graded Dose of Moringa oleifera Seed Extract Administered Orally on Testicular Pathology, Gonadal and Extra Gonadal Sperm Reserves of Wistar Rats Experimentally Infected with Trypanosoma brucei brucei

BACKGROUND: Moringa oleifera seeds are known for their high protein and vitamin content. Antioxidants are abundant in these seeds. Aqueous extraction was done. After that, an acute toxicity test was performed. OBJECTIVES: The purpose of the study was to see how a graded dose of M. oleifera aqueous seed extracts altered testicular pathology, gonadal and extragonadal sperm reserves in Wistar rats infected with Trypanosoma brucei brucei. METHODS: The rats were A, B, C, D, and E were randomly assigned to five groups with group E serving as the control group. The rats in Groups A to D were inoculated intra-peritoneal with 1× 106 virulent T. brucei brucei, and they were held for one week to demonstrate clinical signs before starting the extract therapy. Every day at 10:00 a.m., the rats were given treatment for five weeks with (75, 100, 125, and 150) mg/kg of M. oleifera aqueous seed extract for groups A, B, C, and D respectively. While the control group E received 0.5 mL/kg of water. For hema-tological indices, blood samples were collected every Monday between 10:00 and 11:00 a.m. All of the rats were humanely sacrificed at the end of the six-week experiment, and their gonadal and extragonadal sperm stores were collected, tested, and processed for histopathology. RESULTS: After treatment, the rats' gonadal and extragonadal sperm reserves (groups A to E) showed a substantial increase (213±1.1a; 221±2.1; 250±0.0c; 259±2.6d; 295±2.6e) × 106 and (115±1.1; 160±2.1; 153±0.0; 167±2.6; 120±0.6) × 106 respectively, compared to control group at P < 0.05 level. Sperm concentration of the right epididy-mis (60.0±1.1a; 90.2±2.1b; 96.5±0.0c; 98.7±2.6d; 69.4±0.6e) × 106 were significantly higher compare to the left epididymis (55.0±1.1; 69.8±2.1; 56.5±0.0; 68.3±2.6; 50.6±0.6) × 106. The PCV (%) and WBC (103/μL) levels in groups A, B, and C were significantly greater following infection than that in group E. Infection with T. brucei at weeks 2 and 3 shows poor semen characteristics, thereafter the semen quality has improved. CONCLUSIONS: Moringa oleifera aqueous seeds extract has drastically abridged the impact of trypanosomosis and enhanced the semen quality of the experimental rats.

Moringa Oleifera Seed Extract Concomitantly Supplemented with Chemotherapy Worsens Tumor Progression in Mice with Triple Negative Breast Cancer and Obesity

Triple negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype with limited treatment options. Obesity and insulin resistance are associated with a worse prognosis in those with TNBC. Moringa oleifera (moringa) is a tropical edible plant used for both food and medicinal purposes and found to have anti-obesity and anti-cancer effects in vitro and in preclinical models. The anti-cancer effects of moringa seed extract alone and in combination with chemotherapy were evaluated in immunocompromised female mice with diet-induced obesity bearing MDA-MB-231-derived xenograft tumors. Moringa supplementation protected against high-fat diet- and chemotherapy-induced increases in fasting glucose and improved insulin sensitivity. Moringa supplementation alone did not attenuate tumor growth relative to chemotherapy alone, and in combination worsened tumor progression. Moringa supplementation alone reduced angiogenesis, but this effect was abrogated in combination with chemotherapy. Moringa supplementation may be an effective strategy to improve metabolic health in mice with obesity and TNBC and reduce angiogenesis in tumors, but may have a negative interaction when used as a concurrent complementary therapy. Caution should be taken when considering the consumption of moringa seed extracts while receiving chemotherapy for breast cancer treatment. Further investigations of alternative timings of moringa therapy are warranted.

"Evaluation of Antiurolithiatic Potential of Moringa Oleifera Seed Extract"

Urolithiasis is a debilitating condition in which one or more calculi are formed in any part of the urinary system and the stones...

Protective Effect of Moringa Oleifera Seeds Extract on Toxicity In Liver And Kidney Induced By Acetampride .

The present study aimed to distinguish light on the protective of Moringa oleifera seeds dried ethanolic extract on the toxicity in liver and kidney induced by acetamprid pesticide which widely produced and used for agricultural treatment in Egypt. Physico-chemical parameters and identification of fatty acid composition of Moringa oleifera seeds oil were elvaluated. In addition , the biological effects of bioactive components of seeds dried extract at concentration 200 mg/kg b.w. oil as additives into the diets on kidney and liver functions , lipid profile ,serum glucose levels , hematological parameters and histopatholgical examination of experimental rats were evaluated. From the obtained results , it could be noticed that the total phenolic acid , flavonoids and tannin contents in Moringa oleifera seeds of the dried ethanolic extract were 44.25±1.13 , 114.36±0.72 and 20.77±1.10 ug/g , respectively. Total antioxidant capacity of dried ethanolic extract of Moringa oleifera seeds was found to be 143.29±0.37 , 178.10±2.18 , 189.18±2.06 and 222.47±0.81 at 50 , 100 , 150 and 200 ug/ml . Also, the results showed that the Moringa oleifera (MO) seeds oil extract was dominated by the high percentage of total unsaturated fatty acid (80.29%) than its saturated (19.71%). On the other hand , the results showed that there were significant increasment of biochemical parameters such as serum urea and creatinine (as function of kidney) , as well as ALT and AST activity also albumin and total protein (as function of liver) , total cholesterol , triglyceride and serum glucose levels of the experimental rats. These values were found significantly decreased in all treatments rats fed diets containing MO seeds oils extract at dose of 200 mg /ml b.w. as compared with the group treated by acetampride (toxicated group).The results of the effects of MO seeds extract on hematological parameters of intoxicated rats caused significant decreasments in total white blood cells counts (WBCs) . While, MCV , MCH and MCHC were significantly unchanged relative to those of different from the control group but haemglobin (Hb) and red blood cells (RBCs) was increased. Histopathological studies confirmed chemical analysis results. Finally, from the obtained results and histopathological examination , it could be concluded that the administration of MO seeds dried extract of ethanolic oils might improve the liver and kidney functions in the present experimental rats.