Moringa Oleifera Leaf Research Articles

Haematology and histology studies on Moringa oleifera leaves and bark extracts in streptozotocin-induced diabetic albino rats

Alterations in haematological parameters are frequently observed in individuals with diabetes mellitus (DM). Oxidative stress has been identified as a contributing factor in the pathogenesis of organ damage and haematological dysfunction associated with DM. As a result, the potential positive impact of Moringa oleifera, a medicinal plant known for its antioxidant properties, on haematological parameters and kidney function in diabetic rats was investigated. A total of thirty-five rats were divided into seven groups, each consisting of five rats. Group I was administered only distilled water, while groups II to VII were induced with a single dose of 60mg/kg streptozotocin intraperitoneally. Subsequently, groups II to VI were treated with 150 mg/kg Moringa oleifera leaf methanol extract, 150 mg/kg Moringa oleifera bark methanol extract, 300 mg/kg Moringa oleifera leaf, 300 mg/kg Moringa oleifera bark, and metformin, respectively. The results showed positive modulation to most of the haematology parameters evaluated except neutrophils and lymphocytes by the treatments. The leaf extract showed a more significant modulation of platelets than other treatments. The histology of the kidney showed normal structure for all the treatments. Moringa oleifera especially the leaf extract is promising in the treatment of DM

Growth performance, serum metabolites, plasma protein fraction, meat lipid profile and antioxidant activities of breast meat of broiler chicken fed diets containing Cinnamomum ceylon powder, Zingiber officinale powder and Moringa oleifera leafmeal supplem

An experiment was conducted to determine growth performance, serum metabolites, plasma protein fraction, meat lipid profile and antioxidant activities of meat of broilers as affected by Cinnamomium ceylon, Zingiber officinale and Moringa oleifera supplementation. A total of 112 day old chicks were allotted into four treatments replicated four times with seven birds per replicate in a completely randomized design (CRD). Basal diets were formulated for broiler starters (0-28days) and finisher phase (29-56) days. The basal diets were divided into 4 diets : diet 1- control diet without supplementation; diet 2- contained 0.2% Cinnamomum ceylon supplementation (CCS); diet 3- contained 0.2% Zingiber officinale supplementation (ZOS), diet 4-contained 0.2% Moringa oleifera leafmeal (MOLM) supplementation. The experiment lasted for 56 days. Results showed an improvement in final liveweight, weight gain, feed intake and feed conversion ratio of birds on 0.2% MOLM. Birds on phytogenic feed additives have lower values of urea, cholesterol, glucose, high density lipoprotein and low density lipoprotein. Birds administered phytogenic supplements exhibited much greater plasma albumin levels. Low levels of meat cholesterol, triglycerides and low density lipoprotein were found in birds fed phytogenic feed additives while high levels of high density lipoprotein were recorded for birds on phytogenic feed additives. The supplementation of broilers diets with phytogenic feed additives increased the glutathione peroxidase and superoxide dismutase and decreased lipid peroxidation. It was concluded that supplementation of broiler diets with Moringa oleifera leaf meal enhanced body weight gain, antioxidant activities also reduced cholesterol level in both serum metabolites and meat.

Antibacterial efficacy of Moringa oleifera leaf extract against methicillin-Resistant Staphylococcus aureus isolated from a patient with wound abscesses in Shaafi Hospital, Mogadishu, Somalia

Antibacterial efficacy of Moringa oleifera leaf extract against methicillin-Resistant Staphylococcus aureus isolated from a patient with wound abscesses in Shaafi Hospital, Mogadishu, Somalia

Impact of Maternal Moringa oleifera Leaf Supplementation on Milk and Serum Vitamin A and Carotenoid Concentrations in a Cohort of Breastfeeding Kenyan Women and Their Infants.

Background: Childhood vitamin A deficiency leads to increased morbidity and mortality. Human milk is the only source of vitamin A for exclusively breastfed infants. Dried Moringa oleifera leaf powder (moringa) is a good food source of provitamin A and other carotenoids. Its effect during lactation on human milk vitamin A and carotenoid content is unclear. Objectives: Our objective was to investigate the effect of maternal moringa consumption on human milk retinol and carotenoid concentrations and maternal and infant vitamin A status. Methods: We conducted a 3-month pilot single-blinded cluster-randomized controlled trial in breastfeeding mother-infant pairs (n = 50) in Kenya. Mothers received corn porridge with (20 g/d) or without moringa with complete breast expressions and maternal and infant serum collected at enrollment (infant <30 days old) and 3 months. Milk was analyzed for retinol and selected carotenoids; maternal/infant serum was analyzed for retinol binding protein (RBP). Results: 88% (n = 44) pairs completed milk and serum samples. Four mothers (9%) had vitamin A deficiency (RBP <0.07 µmol/L); 11 (25%) were vitamin A insufficient (VAI; RBP <1.05 µmol/L). Alpha-carotene concentration in milk was higher in the moringa than the control group at baseline (p = 0.024) and at exit (least squares means, LSM, 95%CI µg/mL 0.003, 0.003-0.004 moringa vs. 0.002, 0.001-0.003 control, n = 22/cluster; p = 0.014). In mothers with VAI, alpha-carotene was higher in the moringa group than controls at exit (LSM, 95%CI µg/mL 0.005, 0.003-0.009 moringa, n = 3, vs. 0.002, 0.000-0.004 control, n = 8, p = 0.027) with no difference at baseline. Milk carotenoids did not correlate with vitamin A status (serum RBP) in infants or mothers. Conclusions: Maternal moringa consumption did not impact concentration of milk vitamin A and resulted in limited increase in milk carotenoids in this cohort.

Xylanase and phytase supplementation of Moringa oleifera diets: effects on the performance, egg quality and blood profile of laying hens

Context Moringa oleifera leaves present enormous potential as an alternative feedstuff for laying hens. The utilisation of the leaves can be improved by exogenous enzymes owing to the presence of non-starch polysaccharides and phytate. Aims This study evaluated the influence of Moringa oleifera leaf meal (MOL) in diets supplemented with xylanase and phytase on laying hen performance and egg quality at peak production. Methods In total, 288 Dekalb White laying hens (32 weeks old) were distributed in a completely randomised design, following a 2 × 4 factorial arrangement (with or without MOL × 4 enzyme supplementations). Each treatment comprised six repetitions, each containing six hens. The control diet was based on ground corn and soybean meal, and the treatment diets were supplemented with 5% MOL, followed by three enzyme additives (xylanase, phytase and a xylanase + phytase blend). Key results The enzyme supplementation in MOL diets positively influenced egg production (P = 0.012), egg weight (P = 0.021) and egg mass (P = 0.009). The birds offered xylanase-supplemented diets had higher feed intakes (P = 0.015) than did those fed other diets. Moringa oleifera increased the albumen height (P &lt; 0.001) and Haugh unit (P &lt; 0.001), whereas MOL and phytase improved eggshell thickness (P &lt; 0.001) and the candling score (P = 0.049), compared with treatments without phytase supplementation. MOL and enzyme supplementation improved yolk colour intensity (L, A and R parameters) and decreased plasma total cholesterol and LDL cholesterol concentrations. The inclusion of 5% MOL in diets did not impair laying performance. However, when MOL diets were supplemented with exogenous enzymes, there was an increase in egg weight. Conclusions MOL can be included at 5% in layer diets without affecting performance. When used in combination with xylanase and phytase, they improve performance, intensify yolk colour, and improve shell thickness, candling score, as well as serum biochemical profile. Implications These findings suggest that the combination of dietary MOL + xylanase + phytase can improve performance and egg quality in laying hens at peak production.

Moringa Oleifera leaf-based zinc oxide nanoparticles: Green synthesis, characterization, and their antibacterial investigations

In the current study, an extract from Moringa Oleifera (MO) leaves was utilized for synthesis nanoparticles (ZnO) in an easy-to-use and ecologically friendly manner. Different ratios of MO leaf extraction (1:1, 1:3, 1:5, and 1:7, 1:9) were used to synthesize the nanostructured ZnO. The synthesized ZnO NPs were examined using an array of analytical methods, including energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), UV–Vis absorption spectroscopy, and antibacterial investigations. The synthesized MOL: ZnO NPs confirmed the hexagonal wurtzite structure, as demonstrated by XRD analysis. FT-IR demonstrated that the presence of distinct functional groups was required for the reduction of the metal ion into MOL: ZnO NPs. High purity and crystalline EDX has been used to identify the intense and narrow measurements of the zinc and oxygen found in the MOL: ZnO NPs. After being examined for antibacterial activity, the produced MOL: ZnO NPs showed the highest zones of inhibition against both gram negative Pseudomonas and gram positive Bacillus. The results show that ZnO-based NPs aided by leaf extract of Moringa Oleifera are the promising materials for a variety of applications such as food package applications and advanced forensic applications.

Moringa oleifera, Cyclea barbata, Centella asiatica Leaves Extract Intervention Ability to Improve Fetus Development and Blood Protein Levels in Malnutrition Pregnant Model Mice

Background: Pregnant women experiencing chronic energy deficiency are at risk of giving birth to low birth weight and premature (stunted) babies. Objectives: In an effort to prevent stunting, the study investigates the effects of a 1:1:1 ratio of Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extract on fetal growth and blood protein levels. Methods: This research used a laboratory experimental approach with 25 adult female Mus musculus Balb/C mice as a model of malnourished pregnancy, was divided into 5 groups: pre-test, positive control, negative control, placebo and test group. Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extracts are administered to the test group and positive control in 1:1:1 ratio. Results: The study results indicated that in pregnant mice without malnutrition receiving a mixture of Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extracts, the average fetal body length was 9.7% higher and their weight was 7.4% heavier than pregnant mice with experiencing chronic energy deficiency. Conclusions: The administration of Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extract can contribute to improving fetal development and blood protein levels. However, its effectiveness is more pronounced in normal pregnancies than in pregnancies with chronic energy deficiency as it can prevent stunting.

Moringa oleifera leaf extract ameliorates collagen degradation via the inhibition of MMP‐3 expression in UVB‐induced rats

Prolonged exposure to high‐intensity UVB induces the formation of reactive oxygen species (ROS) in skin tissue, triggering an increase in matrix metalloproteinase‐3 (MMP‐3) enzyme production and leading to collagen degradation. Moringa oleifera (MO) contains bioactive compounds known for ROS‐scavenging and anti‐inflammatory properties. However, the precise molecular mechanism of action remains unclear, requiring the inhibition of MMP‐3 activation and regulation of collagen deposition. This study aims to elucidate the potential effect of MO leaf extract‐based gel in restoring collagen deposition by reducing MMP‐3 activation in UVB irradiate‐induced collagen loss in rats. This study employed a completely randomized design, comprising four groups: a healthy group without UVB radiation, a negative control group subjected to UVB radiation and receiving a placebo, and two treatment groups exposed to UVB radiation with 5% or 10% moringa leaf extract‐based gel (MO‐5% or MO‐10%), respectively. Results showed that MO‐5% and MO‐10% significantly reduced MMP‐3 gene expression and increased collagen density compared to the negative control group (p &lt; 0.05). Moringa oleifera leaf extract ameliorates collagen degradation by inhibiting MMP‐3 expression in UVB‐induced rats, suggesting its potential as a pharmacological and cosmetic agent for UVB‐induced skin damage.

Enhanced supercapacitive performance in Cu doped Sn3O4 synthesized by a simple extract route

The synthesis of nanomaterials using plant extracts is of great importance for a sustainable, chemical-free future. In this study, multivalent Sn3O4 doped with Ni and Cu were synthesized by a simple, low-cost method using Moringa oleifera leaf extract at low temperature and investigated for supercapacitor applications. Various characterizations indicate that the synthesized samples crystallize in a triclinic space group and have a larger surface area of 109.2 m2/g, which is advantageous for electron and ion transport. Electrochemical studies were carried out by cyclic voltammetry, galvanostatic charge/discharge, impedance spectroscopy and cycle life measurements in 6 M KOH electrolyte. Cu-doped Sn3O4 showed the highest capacitance of 608 C/g at a current density of 1 A/g. An asymmetric device built with it delivered an energy density of 31.5 Wh/Kg at a power density of 1400 W/Kg and retained 85 % capacity even after 6000 continuous cycles. The results show that extract-based synthesis of multivalent oxides is beneficial not only for a sustainable environment but also for achieving superior capacity.

Green Synthesis of Carbon Nanodots (CNDs) Moderated by Flavonoid Extracts from Moringa oleifera Leaves and Co-Doped Sulfur/Nitrogen (NS - CNDs - Fla) and Their Potential for Heavy Metals Sensing Application.

Carbon nano dots (CNDs) has received a lot of attention in recent years due to their potential use in various kinds of applications. Many conventional chemical methods have been used to synthesis CNDs, but these processes have several drawbacks, such as hazardous by products, harsh chemicals, and high costs. To overcome these issues, green chemistry has an importance in the development of CNDs. Thus, it is essential to explore green synthesis approaches for production the CNDs. In this context, a novel precursor using flavonoid extraction from Moringa oleifera leaves is proposed. Moringa oleifera is the "magic tree" which contains many bioactive compounds such as flavonoids, especially in its leaves. Natural flavonoids and glycosides with specific components of structure can bind to metal ions. The content of bioactive compounds such as total flavonoids found in Moringa oleifera leaves is 172.10mg/g. In addition, carbon (47.34%), nitrogen (51.67%), and sulfur (0.99%) are also the main composition. Then, the carbon nanodots (CNDs) synthesized in this research were moderated by flavonoid extract from Moringa oleifera using the hydrothermal method. The technique is easy, ecologically friendly, and requires neither specialized device or reagents. In practice, the CNDs produced are almost circular, with an average diameter of 3.49nm. Furthermore, we enhanced synthesis CNDs - Fla with heteroatoms of nitrogen (N) and sulfur (S). They display a broad excitation-emission spectrum, excitation-dependent emission, and extraordinary fluorescence. Therefore, the synthesis of CNDs using flavonoids from Moringa oleifera as precursors would be a great potential candidate for the development of novel types of heavy metals sensing.

An Integration of UPLC-Q-TOF-MS, GC-MS, Electronic Nose, Electronic Tongue, and Molecular Docking for the Study of the Chemical Properties and Flavor Profiles of Moringa oleifera Leaves

Moringa oleifera leaves (MOLs) have gained significant attention due to their nutritional and biological activity. Therefore, this study aimed to examine its flavor characteristics and underlying compositions. In this study, we used ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), gas chromatography-mass spectrometry (GC-MS), electronic nose, electronic tongue, and molecular docking to comprehensively investigate the chemical properties and flavor profiles of MOLs. UPLC-Q-TOF-MS and GC-MS were instrumental in identifying the 20 non-volatile and 19 volatile constituents of MOLs, respectively. The electronic nose and electronic tongue systems provided an objective evaluation of the sweet, bitter, and spicy attributes and flavor characteristics of MOLs. Concurrently, molecular docking was employed to elucidate the material basis of flavor profiles. It revealed that glucosinolates and flavonoids are probably the key components for the bitter taste of MOLs. The sweet taste may be attributed to glucosinolates and flavonoids. The spicy scent appears to be linked to the presence of glucosinolates and alkaloids. The integration of these techniques confers a thorough understanding of the chemical composition and sensory properties of MOLs. These findings have significant implications for innovative applications in the food industry as well as pharmaceuticals and agriculture sectors; furthermore, they contribute towards enhancing the perception of Moringa oleifera as a valuable natural resource.

A Comparative Analysis of Flavonoid and Phenolic concentration from Diverse Varieties of Moringa oleifera Leaves Utilizing Methanol and Ethanol

Having its leaves abundant in nutrients such as vitamins and minerals, the moringa plant is highly regarded because of its nutritious and industrial usefulness. Despite its widespread utilisation, little is understood concerning the way various Moringa oleifera cultivars vary with regard to their phenolic and flavonoid concentration, particularly when extracting the compound utilising various solvents. To attempt to throw insight into the ways that various solvent types and environment variables affects the phytochemical compounds of Moringa oleifera leaves, this research investigation compared the efficacy of ethanol and methanol in removing phenolics as well as flavonoids from the leaves. The information gathered through this research could assist to identify the most appropriate methods for extraction that will maximize the medical and nutritional advantages associated with Moringa oleifera. New leaves of five different types of Moringa oleifera had been bought from the Winfred Thomas Agricultural Research Station in Alabama A&amp;M University. Upon being pulverised and dried, the resulting leaves had been preserved at room temperature. The leaves were immersed in a mixture of 70% ethanol as the solvent and 80% methanol all through the extraction method. The extracted samples had been then stirred, filtered, and freeze-dried. Following that, High-performance liquid chromatography was the technique employed to know the overall concentration of phenolic and flavonoid compounds. Descriptive and statistical analysis were performed using box plots, scatter plots, bar plots, and t-tests to evaluate the extent or degree to which both solvents removed phenolic and flavonoid compounds and estimated the total quantity of phenolic and flavonoids in the different cultivars. The findings from the descriptive study revealed the Nigerian variety had an increased phenolic content when extracted using methanol—roughly 822.3 µg/ml—than when removed using ethanol (80.6 µg/ml). The Indian type, on the contrary, had phenolic concentrations of 814.3 µg/ml as compared with 647.3 µg/ml in ethanol, revealing an important distinction in preference for methanol. The Nigerian cultivar possessed an increased extraction effectiveness of 1253.12 µg/ml using ethanol as opposed to methanol (1083.52 µg/ml) for flavonoids. The t-test results proved that though the difference was not statistically significant (t(28) = 0.608, p = 0.382), the average phenolic content obtained with methanol (M = 798.60, SD = 23.567) was slightly more than that obtained via ethanol (M = 790.60, SD = 45.183). In a similar vein extraction with methanol showed a smaller average flavonoid concentration (M = 1068.40, SD = 128.641) compared to the extraction with ethanol (M = 1129.40, SD = 166.018); nonetheless, this difference was not statistically significant (t(28) = 1.125, p = 0.334). The scatter diagram revealed a significant linear connection with both phenolic and flavonoid content, having a significant positive correlation (Pearson correlation coefficient = 0.95). Although some modifications depending on the variety, the research reveals that methanol and ethanol serve as suitable solvents for phenolic and flavonoid compound extraction from Moringa oleifera leaves. The usefulness of these developments in enhancing methods of extraction in manufacturing industries needs to be explored deeper.

The physicochemical, functional, and pasting properties of Moringa oleifera leaf powder from different leaf stalk colors

ABSTRACT Moringa oleifera (MO) leaf powder (MOLP) is functional, and its composition affects its use. The MO tree has different colors i.e. white, green, and red. This study aimed to evaluate MOLP physicochemical, functional, and pasting properties based on the leaf stalk color as the basis for their suitable use in food products. Leaf stalk color slightly affect characteristics, with typical high protein, dietary fiber, and ash, but low starch and fat. MOLP has high soluble dietary fiber, high amylose starch, and significant amounts of phenolics and flavonoids. Chlorophyll is the abundant pigment followed by β carotene. MOLP is rich in essential amino acids with methionine is a limiting essential amino acid. MOLP has almost equal saturated and unsaturated, and rich in essential fatty acids. Low starch and high fiber affect pasting properties, exhibiting low viscosity and gelatinization temperature, but high stability. Low solubility of MOLP is a challenge to improve.

Hermetic effect of Moringa oleifera leaf extract mitigates salinity stress in maize by modulating photosynthetic efficiency, and antioxidant activities

Salinity poses a significant constraint to cereal productivity particularly in arid and semiarid regions. The application of allelochemical has shown promising results in mitigating the intensity of abiotic stresses. A pot experiment was conducted to assess the efficacy of different concentrations of aqueous allelopathic extract derived from moringa leaves in mitigating the adverse impacts of salinity on the germination and growth of maize cultivars via seed priming. The study involved three variables: two cultivars of maize, ‘Pioneer 30Y87’ (salt tolerant) and ‘Pioneer 30T60’ (salt sensitive) e seed priming with moringa leaf extract (MLE) at varying concentrations of 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, and hydro-priming as control; and different salinity levels of 0, 6, and 12 dS m-1. Salinity had a negative impact on the germination process, leading to delayed and suboptimal growth of seedlings. Additionally, salinity reduced the synthesis of photosynthetic pigments (20-50%), photosynthesis, transpiration, internal carbon, and stomatal conductance. Further, MLE also improved the antioxidant activities (catalase: CAT and peroxidase: POD) by 22-56% which reduced the hydrogen peroxide production. Moreover, ‘P-30Y87’ exhibited favorable performance in terms of better germination, growth, photosynthesis and antioxidant activities. The application of moringa leaf extract (3%) resulted in a more notable hermetic effect in elevating salinity stress thereby enhancing germination, growth, photosynthesis and antioxidant activities. In the conclusion, application of MLE (3%) is a promising approach to mitigate the adverse impacts of salinity by improving germination, growth, photosynthesis and antioxidant activities.

Antifungal activity of moringa (Moringa oleifera) leaf extracts against major plant pathogens

Antifungal activity of moringa (Moringa oleifera) leaf extracts against major plant pathogens

The Effects of Moringa Leaf Extract on the Growth and Microbial Load of Boiler

The adverse effect of microbes in poultry production, especially broiler chicken remains a major concern for poultry breeders. This study determined the effects of Moringa leaf (Moringa Oleifera) extract on the performance and microbial loads of broiler birds. A total of 25 broiler chicks were used and separated into 5 groups in an iron pen with 5 chicks in each group. Group 1 (control, with no treatment), group 2 (received a treatment of 62.5mg/L), group 3 (received a treatment of 125mg/L), group 4 (received a treatment of 250mg/L), group 5 (received a treatment of 500mg/L). After 4 weeks of treatment, the faeces from each group, the control group inclusive were collected for microbial analysis. The result showed that the Moringa Oleifera leaf extract at all doses recorded non-significant difference in the parameters analysed among the groups. It was also noticed that the disparities of the results were great. Some as much as 11.5 and 168. 37 standard error of mean. Moreover, Group 3, receiving 125mg/L, exhibited a substantial reduction in E. coli, salmonella, total bacteria count, and pseudomonas activities compared to other groups. Group 3 had the highest water consumption. Amongst the treatment group, group 3 had the highest increase in weight. In conclusion, Moringa leaf extract effectively lowered the microbial load in broiler chicks at the treatment of 125mg/L without any adverse effects. Keywords: Moringa Oleifera, Moringa leaf, microbial load, broiler birds.

Moringa oleifera and Allium sativum in broiler nutrition: carcass traits, biochemical characterization, and economic analysis

To ensure the productivity and economic viability of broiler chicken, this study was designed to determine the impact of Moringa oleifera leaf powder (MLP) and Allium sativum bulb powder (ABP) utilization on carcass trait, biochemical characterization and economic value of broiler chickens. Two hundred and forty (n = 240), 8-day-old (Cobb 500) broiler chickens were randomly allotted to 5 treatments with 4 replicates (12 birds/replicate) in a completely randomized design (CRD). The birds were randomly allotted into five groups: T1: diet without MLP and ABP, T2: diet with MLP replaced soybean meal (SBM) at 1%, T3: diet with MLP replaced SBM at 3%, T4: diet with MLP replaced SBM at 1% + 0.1% ABP and T5: diet with MLP replaced SBM at 3% + 0.3% ABP respectively. Each formulated diet was fed ad libitum from the 2nd to 8th week of age. Tannins, phenols, saponins, flavonoids, and reducing sugar were present in MLP and ABP. The percentage dressed weight on the 27th day in T2 and T3 were (P < 0.05) higher than in T1, but comparable (P > 0.05) to those in T4 and T5. On the 56th day, the breast weight in T5 was (P < 0.05) higher than in T1 and the rest of the treatments. Most of the microbes that were mainly identified from the ceca of birds fed dietary treatments were Staphylococcus species, and Streptococcus species. Production cost was significantly (P < 0.05) reduced in T3 (₦1, 843.76) than in T1 (₦1, 905.86) and the rest of the treatments. Therefore, it is economically wise to feed broiler chicken with MLP and ABP, but most economical when a fed diet with MLP replaces SBM at 1% and a diet with MLP replaces SBM at 3%.

EFFECT OF MORINGA (Moringa oleifera) LEAF EXTRACT ON IN VITRO INHIBITION OF Candida albicans BIOFILM

Background: Oral candidiasis is an opportunistic infection in the oral cavity. This infection is caused by the fungus Candida albicans. The most important virulent attribute of this fungus is its ability to form biofilms, which can adhere to mucosa, epithelial lining, organs, prostheses or dentures. The formed biofilm is resistant to antifungal drugs. Objectives: The purpose of this study was to determine the effect of Moringa (Moringa oleifera) leaf extract on the inhibition of Candida albicans biofilm formation in vitro. Methods: This type of research is experimental study using post tests with control group design. Fungus used in this study was Candida albicans ATCC 10231. Determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were conducted by microdilution method. Inhibition test of C. albicans biofilm formation was carried out using the polystyrene microplate assay method. Concentration of extracts used in this study were 12.5%, 6.25%, 3.13% and 1.57%. Inhibitory activity of biofilm was measured using a crystal violet (CV) assay. Results: The results showed that MIC of Moringa leaf extract against the fungus C. albicans was at a concentration of 6.25%. Moringa leaf extract starting at a concentration of 3.13% already has had ability to inhibit formation of C. albicans biofilm. Conclusion: It can be concluded that Moringa leaf extract can inhibit formation of Candida albicans biofilm, so it can be developed as an alternative herbal ingredient to prevent oral candidiasis.EFFECT OF MORINGA (Moringa oleifera) LEAF EXTRACT ON IN VITRO INHIBITION OF Candida albicans BIOFILM

Antibacterial, antioxidant and anticoagulant activities of different aged Moringa oleifera leaf extracts and application in natural serum product

Antibacterial, antioxidant and anticoagulant activities of different aged Moringa oleifera leaf extracts and application in natural serum product

Assessing the usefulness of Moringa oleifera leaf extract and zeatin as a biostimulant in enhancing growth, phytohormones, antioxidant enzymes and osmoprotectants of wheat plant under salinity stress

Assessing the usefulness of Moringa oleifera leaf extract and zeatin as a biostimulant in enhancing growth, phytohormones, antioxidant enzymes and osmoprotectants of wheat plant under salinity stress