Pharmaceutical effluents containing fluroquinolones (FQs) are one of the incipient organic impurities in aquatic environment. The ecosystem and human health were seriously affected from the remaining FQs in marine environment because of their toxicity. Hence, elimination of this FQs by emerging adsorbents with higher efficacy needs to be developed. This current research intends to developed a novel chemically modified jarul biochar/polypyrrole (CLSHB/PPy) composite via pyrolysis followed by sodium hydroxide modification and oxidative polymerization of pyrrole monomer to sequestrate ofloxacin (OFX) from aqua matrix. The characterization results of fabricated composite revealed that agglomerated spherical shape particles and their crystallinity nature can be act as an adequate adsorbent. Several sorption progression limitations are optimized which impact the interaction between CLSHB/PPy and OFX species. The optimum OFX eradication (%) of 82.42 ± 0.90 % by CLSHB/PPy was detected at pH 7.0, dose 1.0 g/L, and initial concentration 60 mg/L. Furthermore, Langmuir isotherm exhibited well fitted (R2 = 0.99) investigational data with an elimination ability of 50.02 ± 0.65 mg/g. Besides, kinetic analysis exposed that Pseudo second order model contribute the best suited kinetic. Thermodynamic study revealed that sorption progression of OFX onto CLSHB/PPy was both spontaneous and endothermic (∆H° ˃ 0). Moreover, CCD optimization exposed that investigational outcome (84.81 ± 1.02 %) are also adjacent to model integrated outcomes (84.12 ± 0.85 %), which indicates importance of RSM analysis. Stability analysis of composite (CLSHB/PPy) has been executed and exposed that the composite material can be effectively reutilized for OFX sorption after certain cycles.