This study investigates the presence and diversity of monogenean parasites infecting Nile tilapia (Oreochromis niloticus) in the Ghaghara River, recognizing their significant impact on fish health. Through field surveys, Nile tilapia specimens were collected from various locations along the river and subjected to standard parasitological analyses to identify monogenean parasites. Morphological assessments facilitated species identification and diversity assessments. Preliminary findings reveal a diverse array of monogeneans infecting Nile tilapia in the Ghaghara River, with species richness and prevalence varying across sampling sites, possibly influenced by environmental factors. While some monogenean species exhibit host-specificity, others demonstrate a broader host range. Understanding the distribution and diversity of these parasites would enhance our understanding of fish health and Ghaghara River ecosystem dynamics, informing management strategies for sustaining robust fish populations and promoting eco-friendly aquaculture practices in the region. Moreover, insights gained from this research may contribute to the conservation and management of aquatic ecosystems beyond the Ghaghara River.