Monocyte-derived Chemotactic Factor Research Articles

Lucigenin-induced chemiluminescence in human neutrophils in the process of chemotactic migration measured in a modified Boyden chamber.

To the question of whether an oxidative metabolism of neutrophils occurs in the process of chemotactic migration, we have already demonstrated that formyl-peptide and zymosan-activated serum effectively induced lucigenin-dependent chemiluminescence (CL) in a specially devised Boyden chamber. In the present study we confirmed this and, furthermore, demonstrated that superoxide dismutase partially suppressed the neutrophil chemotaxis to formyl-peptide and concanavalin A, suggesting the participation of superoxide in a chemotactically migrating mechanism. However, monocyte-derived chemotactic factor did not cause any light emission, irrespective of its chemotactic activity. Based on these results, neutrophil chemotactic factors seem to be divided into two types, that is, oxidative metabolism-related and nonrelated. To the former group of chemotactic factors a premature activation of oxidative metabolism in neutrophils may start even at the initial stage of chemotaxis, and these primed cells may respond with a respiratory burst to higher concentrations of the chemoattractant itself or to other chemical mediators present at the site of inflammation.

Function of phagocytes in experimentally induced colitis in rabbits. II. The Shwartzman phenomenon in the colon.

Adhesion, phagocytosis, chemotactic and random migration, nitroblue tetrazolium dye reduction of peritoneal exudate neutrophils and macrophages, fibrinogen level, gelation of soluble fibrin and serial dilution protamine sulfate test were investigated in 115 New Zealand white rabbits with experimentally induced Shwartzman phenomenon in the colon, and in control animals. The results presented in this report demonstrated impairment of chemotactic migration of phagocytes in the presence of endotoxin. The depression was dose-dependent and less marked when neutrophils were stimulated with monocyte-derived chemotactic factor or with casein, than with complement-derived chemotactic factor. The prolonged depression of chemotactic migration of neutrophils and macrophages in rabbits with colitis, however, did not affect the healing time of the ulcers in the colon.