Momordica Dioica Research Articles

Enhancement of nutritional, functional, and bioactive properties of Dhokla mix through incorporation of spine gourd (Momordica dioica) powder: A comprehensive analysis

Enhancement of nutritional, functional, and bioactive properties of Dhokla mix through incorporation of spine gourd (Momordica dioica) powder: A comprehensive analysis

Evaluation of Beta-cell Regeneration Property of Methanolic Extract of Momordica Dioica in Wistar Rat Model

Background: Despite the existence of various medications for diabetes, many individuals still struggle with the condition due to the potential side effects associated with current treatments. Traditional healers have long relied on medicinal plants with potential antidiabetic properties. One such plant is Momordica dioica, although its traditional use lacks experimental studies to support its efficacy in managing diabetes. Among them, Momordica dioica is one however there are no experimental studies on beta-cell regeneration that support the current traditional uses. Objective: The main aim of the current research is to identify Momordica dioica's methanolic extract's capacity for beta cell regeneration. Methods: Air-dried Fruit of Momordica Dioica was extracted using methanol, and acute oral toxicity studies were performed on methanolic extract in accordance with OECD guideline version 18. By using the HPTLC method, the Active Constituent in charge of the activity was verified. The HPTLC method was used to confirm active constituents that were responsible for the activity. The pancreatic beta cell regeneration as well as Fasting blood glucose level was used to evaluate the anti-diabetic effect. Single-dose STZ-induced type 2 diabetes mellitus was used to evaluate anti-antidiabetic activity. Results: Momordica dioica’s methanolic extract with doses of 100mg/kg and rest combination with standard showed a significant (p < 0.0001) anti-diabetic effect. Maximum anti-diabetic effects at doses as HE100mg/kg and all combinations were compared to standard and observed from Day 21 induction of diabetes by Streptozotocin; total tested doses of the extract inhibited the destruction of beta-cell significantly (p < 0.0001, p < 0.001). Conclusion: The existence of berberine, a quaternary ammonium salt of the alkaloid benzyl-iso-quinoline present in the extract, had a potential anti-diabetic effect and beta-cell regeneration property which supports the ancient beliefs.

Effect of Drying Methods and Temperature on Physical Properties of Spine Gourd (Momordica dioica Roxb.)

The present investigation entitled “Effect of Drying Method and Temperature on Physical Properties of Spine Gourd (Momordica dioica Roxb.)” was conducted at the Post Harvest Technology Laboratory, Department of Horticulture, Rajasthan College of Agriculture and Department of Processing and Food Engineering, College of Technology and Engineering, MPUAT, Udaipur, Rajasthan during August to December, 2023. The study consisted of 4 different drying methods (sun drying, tray drying, heat pump drying and fluidized bed drying), pretreatment (water blanching) and different temperatures (50°C, 60°C and 70°C). The experimental design used for this study was completely randomized design with three replications. The various physical observations were also recorded after drying. Among different types of drying methods and temperatures, water blanched and dried at 60°C tray dryer was found best with maximum dehydration ratio (0.174) and recovery % (17.43).

Chemo-profiling and exploring therapeutic potential of Momordica dioica Roxb. ex Willd. for managing metabolic related disorders: In-vitro studies, and docking based approach

Ethnopharmacological relevanceMomordica dioica Roxb. ex Willd. (M. dioica Roxb.) a nutritious and therapeutic property rich crop of Cucurbitaceae plant family. In various folklore medicine including Ayurveda fruits are used to treat several metabolic related disorders i.e., hyperglycemia, hyperlipidemia, diabetes, obesity etc. Furthermore, traditionally it is used to treat fever, inflammation, ulcer, skin diseases, haemorrhoids, hypertension and also employed as cardioprotective, hepatoprotective, analgesic, diuretic. Aim of the studyThis study focuses to explore the therapeutic potential of Momordica dioica Roxb. ex Willd. through in-vitro and in-silico approach for managing hyperlipidemia, hyperglycemia and related metabolic disorders along with its phytochemical profiling for quality evaluation and validation of traditional claim. Materials and methodsThe present study was carried out on hydroalcohol extract of dried leaf and fruit of Momordica dioica. In-vitro antioxidant potential using DPPH and Nitric oxide scavenging assay along with in-vitro enzyme inhibitory potential against α-amylase, α-glucosidase, and pancreatic lipase enzymes was studied. The bioactive metabolites were identified from the most potent bioactive extract by analysis with LC-QTOF-MS and also studied their role to lessen the metabolic related disorder through in-silico approaches. ResultsThe results confirmed that the fruit extract is more active to possess antioxidant and prominent enzyme inhibition potential compared to the leaf. Sixteen identified metabolites in M. dioica Roxb. fruits may be responsible for the therapeutic potential related to metabolic related disorder. The in-silico study of the identified phytomolecules against α-amylase, α-glucosidase and pancreatic lipase showed significant docking scores ranging from −9.8 to −5.5, −8.3 to −4.8 and −8.3 to −6 respectively. ConclusionThe current study illustrated that M. dioica Roxb., a traditionally important plant is potential against metabolic related disorders. Phytocomponents present in the fruit extract may be responsible for antioxidant as well as the enzymes’ inhibitory potential. Thus, fruits of M. dioica Roxb. will be useful as alternative therapeutics for treatment of hyperlipidemia, hyperglycemia and related metabolic disorders.

Momordica dioica: A medicinal plant, its benefits and pharmacological activity

Momordica dioica is a cucurbitaceous climbing creeper (commonly known as kakrol, spiny gourd or teasle gourd). It is native to Asia with widely distributed in India and Bangladesh also. It is used as preventive and curative agent for various diseases. It has also significant nutritional value as vegetable over thousands of years. This review aims to take an attempt to evaluate the pharmacological properties and benefits of Momordica dioica according to the view of traditional medicinal plant based on treatment including. Momordica dioica is considered as an underutilized vegetable, although having significant presence of certain compounds containing higher nutritional value than many frequently consumed vegetables. Different plant parts of Momordica dioica provide a numbers of phyto-constituents like alkaloids, glycosides, steroids, triterpenoids, flavonoids, ursolic acid, vitamins, minerals and rich in fibre. They have potential to cure asthma, excessive salivation and inflammation caused by lizards, snake bite, elephantiasis, fever, mental and digestive disorders. It is used to maintain skin health.

Development of female specific SCAR marker in spine gourd (Momordica dioica Roxb. Ex. Wild) and its validation

Momordica dioica is a dioecious vegetable crop belonging to the family Cucurbitaceae. Fifty RAPD primers were used to analyze the male and female DNA bulks consisting of six females and six male genotypes of Momordica dioica respectively and 23 of them have differentiated the reproductive DNA bulks. One of the primers, OPB07, amplified a female reproductive bulk specific fragment of 326 bp (OPB07326) and was also present exclusively in all female genotypes used to prepare the reproductive bulk. This unique fragment OPB07326 was cloned and sequenced and was developed into female specific SCAR marker MdFSOPB07255. This SCAR marker has also amplified the unique female-specific fragment of 255 bp in all the six female genotypes used for the preparation of the bulk. The existence of this female-specific fragment in M. dioca genome was also affirmed by southern hybridization of DIG-labeled probe of OPB07326 with EcoRI restricted profile of all the six female genotypes. Further, the MdFSOPB07255 marker was successfully employed to identify eight female genotypes out of ten M. dioica genotypes with unknown reproductive feature. Thus, MdFSOPB07255 could be utilized as an efficient and reliable DNA marker for early differentiation of reproductive type at the sapling stage.

A Review on Effect of Bio-fertilizers and Chemical Fertilizers on Growth, Yield and Quality of Dioecious Cucurbits

No vegetable group in India is as diverse as the cucurbits. They are adapted to diverse soil and climate condition and contribute significantly to total vegetable production. Dioecious cucurbits cultivation merge with trellising, staking, mulching, application of biofertilizers and growth regulators hold immense potential and promise for future vegetable production and market in our country. Biofertilizer can be defined as a substance which contains living organisms which, when applied to seed, plant surface, or soil, colonize the rhizosphere or interior of the plant, and promote growth by increasing the supply or availability of primary nutrients to the host plant. Biofertilizers are an chief component of integrated nutrient management system for sustainable agriculture and along with chemical fertilizers. Studies suggest that cultivation of dioecious cucurbits along with chemical and organic fertilizers gives immense result on account of yield and productivity. Our country possesses a rich diversity like spine gourd, ivy gourd, bitter gourd, sweet gourd, pointed gourd and has ideal climate and soil to cultivate these cucurbits. Mostly these miner cucurbits are largely grown or wild gathered and sold in the market by small and marginal farmers. Hence promotion of these cucurbits holds key to ensuring livelihood security and income generation for to the tribal’s, marginal and small farmers in remote rural regions. Dioecious cucurbits mainly possess different important chemical compounds which can be used in nutraceuticals industries. These cucurbits has the potential to act as a source of income generation for rural farmers as they have immense market potential. These cucurbits need strong research support for better production and utilization.

Upcycling Surinam cherry and spine gourd fruit waste: development of anthelmintic jelly candies using fruit extracts.

The online version contains supplementary material available at 10.1007/s13197-024-05967-5.

Organic Farming: The New Success Mantra for Sustainable Agriculture

Organic farming avoids investment in costly chemicals and pesticides. Mrs Neerukonda Satyanarayana adopted organic farming from the year 2015-16. He attended various trainings organized by the Department of Agriculture and Krishi vigyan Kendra, Venkataramannagudem. After that he prepared 14 types of organic inputs, bio fertilizers, bio pesticides on his farm. Banana, Papaya, Vegetables (Brinjal, Tomato, chilli and Spine gourd) and Black gram crops were cultivated in 10 acres area by using the organic inputs. He got a net income of Rs.3 lakhs /ac from Vegetable cultivation. In the case of bananas, he got 1.5 lakhs /ac net income. For Black gram, Rs. 35000/ac net income, and for papaya, 1.5 lakhs.

Amelioration of economically important traits through mutagenesis in spine gourd (Momordica dioica)

Mutation breeding can sometimes be endeavored along with conventional breeding efforts, resulting in the discovery of some economically useful genetic changes. Evaluation of 96 first-generation mutant germplasm lines of spine gourd (Momordica dioica Roxb.) was done through morphological descriptors and molecular makers. Correlation coefficient analysis, path analysis and cluster analysis supplemented by molecular marker-based population structure analysis helped in understanding the population behavior and identification of high-yielding lines. The yield was found to be highly positively correlated with days to first flowering, leaf width, single fruit weight and number of fruits per plant. Also, the maximum positive direct effect on yield was shown by single fruit weight followed by the number of fruits per plant and leaf width. The population structure analysis revealed 4 clusters of population. Few high-yielding lines were identified which approximately yielded 1.5 times more than the checks. Further refinement in these mutant lines through breeding programs coupled with in-depth molecular analysis can lay the foundation of the release of some excellent spine gourd varieties in the future.

Evaluation of Anti-Inflammatory and Analgesic Property of Methanolic Extract of Momordica Dioica in Wistar Rat Model

Background: Since current medications frequently cause potentially serious side effects, countless individuals continued to have pain and inflammation despite the accessibility of treatments. Ancient practitioners have used a variety of herbal remedies with analgesic and anti-inflammatory properties extensively. Momordica Dioica is one of them, but no experimental research has been done in support of this traditional use. Objective: The purpose of the research was to assess the analgesic and anti-inflammatory properties of methanolic Momordica Dioica fruit extracts. Methods: Dryed by air According to OECD guideline the version eighteen, the fruit of Momordica Dioica is extracted with the solvent methanol as well as an acute oral toxicity study was done for the methanol-based extract of Momordica Dioica. The Active Constituent responsible for the Activity was confirmed by HPTLC method. The hot plate method was employed to assess the analgesic efficacy of centrally mediated analgesia.The ability to reduce inflammation was assessed using a carrageenan-induced paw oedema test. Results: Momordica dioica's methanolic extract inhibits the ceiling result during activity. The Methanolic Extract of Momordica dioica (MEMD) extract demonstrated a significant (p 0.0001) analgesic effect in the hot plate method at doses of 100mg/kg and in all combinations with standard. Maximum anti-inflammatory effects were seen in carrageenan-induced paw edema starting 2-4 hours after induction, and all tested doses of the extract significantly inhibited the carrageenan-induced inflammation (p 0.0001, p 0.001). The presence of a pentacyclic terpenoid i.e Ursolic acid present in the extract had potential analgesic and anti-inflammatory activity which supports the traditional claim.

Effect of different seed treatments to promote germination in spine gourd (Momordica dioica Roxb.)

Effect of different seed treatments to promote germination in spine gourd (Momordica dioica Roxb.)

Morphological and physiological changes (variation) in colchicine induced tetraploids of spine gourd (Momordica dioica Roxb.) in comparison to their diploid counterparts

Morphological and physiological changes (variation) in colchicine induced tetraploids of spine gourd (Momordica dioica Roxb.) in comparison to their diploid counterparts

Estimation of Ursolic Acid in Methanolic Extract of Momordica dioica by HPTLC Method

Estimation of Ursolic Acid in Methanolic Extract of Momordica dioica by HPTLC Method

Protein and Total Free Amino Acid Content Evaluation of Improved and Local Varieties/Germplasm of Spine Gourd Momordica dioica Roxb. (Family - Cucurbitaceae)

Spine gourd (Momordica dioica Roxb., 2n = 2X= 28) is a nutritionally rich, perennial and dioecious cucurbit with good assortment of adaptability. Varieties of spine gourd improved germplasm/varieties collected from farmers' field and were sown in Completely Randomized Design (CRD) with Three replications at Acharya Narendra Deva University of Agriculture and Technology Ayodhya, Uttar Pradesh, India during monsoon season 2020. Biochemical characteristics of spine gourd analysis revealed that maximum Protein content (3.09mg/100g) was found in variety Kheri-3,1. Total free amino acid content (6.90mg/100g) was found in variety NDMD-2. Minimum protein content was found in the variety Arka Bharat (2.94mg/100g) and Total Free Amino acid content was reported in the variety Ambika-K-13,6 (6.27mg/100g). Based on observation, variety Kheri-3,1 and NDMD-2 were found superior in both Protein and Total Free Amino acid parameters followed by germplasm Arka Bharat and Ambika-13,6 respectively.

Evaluation of Teratogenic Effect of Methanolic Extract of Fruits of Momordica dioica In Rat Model

Background: Teras- ‘Monster’ and Genesis- ‘Producing.’ Teratogenecity is the ability to cause fetal abnormalities when administered to the pregnant mother at any stage of pregnancy. Momordica dioica, commonly known as spiny gourd, is a species of flowering plant in the Cucurbitaceae family. It has commercial importance and is exported as well as used locally as a vegetable in all regions of India. Objective: The main objective of this research was to evaluate the teratogenic activity of methanolic extract of fruits of Momordica dioica in rat model. Methodology: The dose selection was made based on acute toxicity studies. For evaluating teratogenicity, the pregnant animals were divided into 5 groups and drug administration was done from day 6-17 of gestation. All groups were subjected to laparotomy and then were sacrificed for teratogenic examination which involves physical parameters, morphological analysis and histopathological studies of the foetuses. Results: The rats treated with methanolic extract of fruits of Momordica dioica did not show any teratogenic activity which was evident by observing the physical parameters, morphological analysis and histopathological study. Conclusion: From the results we can conclude that methanolic extract of fruits of Momordica dioica administered at a dose of 250, 500, and 1000mg/kg bodyweight was not teratogenic.

Design, Optimization and Characterization of Newer Herbal Tablet Containing Momordica dioica Extract

Design, Optimization and Characterization of Newer Herbal Tablet Containing Momordica dioica Extract

Development and Validation of Analytical Method for Estimation of Berberine in Herbal Extract of Momordica Dioica

Background:While several anti-diabetic medications have been found to lower blood glucose levels, there are numerous negative effects connected with the use of currently marketed anti-diabetic medications.However, there are medicinal plants with anti-diabetic effects with low or no side effects. Among the Cucurbitaceae family of plants, Momordica dioica is a dioecious climbing herb. It contains many phytoconstituents.One of it is a quaternary ammonium salt of benzyl-isoquinoline alkaloid called as berberine exhibiting a wide variety of pharmaceutical properties.In the current investigation, the activities of berberine for its anti-diabetic potential in the Momorica Dioica plant.The goal of the recent effort is to establish and validate an HPTLC method that is quick, accurate, exact, and specific for determining berberine from herbal extract of Momordica dioica. Methodology/Conclusions/significance: For a quick examination to determine the amount of berberine, a (HPTLC) process was created as well as validated.On HPTLC aluminium plate 60 F254,precoated with silica gel chromatographic separation was accomplished using the solvents: methanol ,ethyl acetate, toluene and formic acid (2:4:3:0.5). A wavelength of 348 nm was used for detection. Rf was found to be 0.44±5% for berberine. There was linearity for berberine in the 400ng/band concentration range.The LOD and LOQ was found to be0.0096ng/band and 0.0293ng/ band for Berberine.The average berberine recovery rate was (0.041).The method's precision, accuracy, linearity, robustness, and specificity were all authenticated in accordance with ICH guidelines. The discovered approach can be used to regularly analyse Berberine in herbal formulations for quality control.

Efficacy of score deviation method as a novel sensory evaluation technique for the identification of optimal mixed vegetable soup formulations

This work demonstrates the formulation, characterization and identification of optimal soup mixes through a structured methodology which equally prioritized both nutritional and sensory aspects. To achieve this, a novel sensory evaluation technique named as Score deviation method was designed. This method was implemented in conjunction with the conventional 9-point hedonic scale method to achieve optimal soup formulations with increased confidence. A total of forty soups formulated with various combinations of summer and winter vegetable produces were evaluated for the sensory properties by a semi-trained panel of 15 subjects utilizing both the methods. All the developed formulations were compared with a commercial soup product which was taken as a control for the study. Finally, a few summer and winter vegetable formulations were screened based on the deviation index values obtained through score deviation method and sensory scores obtained through both the methods. Deviation index values which indicated the extent of uniformity among the sensory parameters played a key role in better screening of the formulations. Lastly, among all summer vegetable soups, with high sensory scores, lowest deviation index and high nutritional composition, formulation with 1 g spine gourd, 1 g sponge gourd, 0.5 g yardlong bean, 2.5 g corn flour and 5 g spice mix was identified as the best soup mix. Among all winter vegetable soups, formulation with 1 g ash gourd, 1 g bottle gourd, 0.5 g squash, 2.5 g corn flour and 5 g spice mix was identified as the best soup mix.

Ethnobotanical and Phytochemical Study of members of Cucurbitaceae from Ralegaon Region-A Review

The plant species belonging to Cucurbitaceae family have a worldwide distribution, but most species can be found in tropical and subtropical countries. A number of the plants belonging to this family have reported important and biological pharmacological activities. Cucurbitaceae member plants are also in use in the folk medicinal system of India. The members of the Cucurbitaceae are annual or perennial herbaceous plants having climbing habit with characteristic tendrils. Plants of this family have many medicinal and nutritional benefits. Therefore, it is important to find out the Ethnobotanical uses in rural region of Ralegaon area and containing active agents possessing pharmacological activity in plants. In this study, we have documented some of the important plants viz., Citrullus lanatus, Lagenaria siceraria, Bryonia lacinosa, Cucumis sativa, Coccinia grandis, Cucurbita pepo, Momordica dioica, Momordica charantia, Cucumis callosus, and Luffa acutangular located in Ralegaon region.