Molise National Park Research Articles

Estimating body condition of Apennine brown bears using subjective scoring based on camera trap photographs

The assessment of animal body condition has important practical and management implications for endangered wildlife populations. The nutritional condition of a population can be evaluated in a non-invasive way using photogrammetry techniques, avoiding direct manipulation. This study evaluates the utility of using body condition scoring (BCS) based on the visual assessment of subcutaneous fat and muscle from the body contour as a non-invasive method to quantify body condition in free ranging bears from camera trap photographs. Photographs of Apennine brown bears (Ursus arctos marsicanus), taken between 2007 and 2009 in the Abruzzo, Lazio and Molise National Park (PNALM, Italy), were used to evaluate the potential of this technique. BCS assessment was performed on 754 photographs representing 71 independent observations. Forty-eight of these photographs were selected to also score quantitative body ratios using a standardised measure of torso height. BCS varied seasonally, as expected by food availability and brown bear nutritional physiology, and it was also positively correlated to all three body ratios. Our findings indicate that BCS assessment is a good proxy for body condition, and that camera trap data can be effectively used to assess and monitor the nutritional condition of bear populations, such as the critically endangered one in central Italy.

The geodiversity of the Abruzzo, Lazio, and Molise National Park (Central Italy)

ABSTRACT This paper presents a novel geodiversity map of the Abruzzo, Lazio, and Molise National Park (Central Italy). The Park exhibits a wide range of geological-geomorphological environments of great scientific and touristic interest, offering unique landscapes and wild nature frequented by visitors year-around. All these features reflect a marked geodiversity and a valuable geoheritage, requiring protection and valorization activities. The geodiversity map that we present was realized following an integrated methodological approach, which includes and combines geological-geomorphological features, geoheritage elements, data from Park archives, and new field-based observations. In this framework, the map serves as a key outreach tool for the dissemination of the geological information of the area and for the promotion of its geodiversity through a modern, sustainable, and environmentally aware tourism in an highly frequented protected area.

Pedicularis rostratospicata subsp. marsica (P. Sect. Rostratae, Orobanchaceae), a New Subspecies from the Central Apennines (Italy).

The new subspecies Pedicularis rostratospicata subsp. marsica is here described based on morphological and molecular analyses. The new taxon is endemic to few localities of the Central Apennines within the Abruzzo, Lazio and Molise National Park (Central Italy). Pedicularis rostratospicata subsp. marsica can be distinguished from the other two currently accepted subspecies (subsp. rostratospicata and subsp. helvetica) by their taller stems, shorter petioles of basal and middle leaves, narrower blades of basal leaves, longer inflorescences with longer internodes and more flowers, and longer calyx lobes. Molecular analysis confirmed the autonomy of the new taxon. Furthermore, the conservation status assessment of the new subspecies according to IUCN categories and criteria is proposed and discussed, and an analytical key to the three subspecies of P. rostratospicata is presented.

Assessing forest accessibility for the multifunctional management of protected areas in Central Italy

Multifunctional forest management should provide the opportunity to create, conserve, modify or eliminate forest roads. Within protected areas, it is difficult to make a single assessment of the degree of accessibility to different forest areas, having to mediate among productive forestry, protection needs and other benefits deriving from forest stands. A GIS-based methodology, with the support of a Forest Information System (FIS) and available Forest Plans for the study area, were applied to create an accessibility map (based on the forest roads network) for the Abruzzo, Lazio, and Molise National Park (PNALM). Results were related to several FIS metadata, highlighting that accessibility in the study area was sufficient, but not optimal, in the productive management units, being rather poor in those where soil protection and biodiversity conservation are the main functions (only 38.8% of them were accessible). Forest roads density (28.5 m ha−1) was not homogeneously distributed within the study area and the ratio between forest road length (199.4 km) and planned forest surface (13,355.3 ha) is only 14.9 m ha−1. In contrast to what is commonly found in forest accessibility works, the innovative element of this study was the involvement of PNALM’s technical office in evaluating the results and exploring the opportunity to adopt a different policy for forest roads network management.

Forest Dynamic in the Italian Apennines

In recent decades, many mountain areas of the Mediterranean countries show spontaneous reforestation or densification due to depopulation and the consequent abandonment of traditional agricultural and pastoral activities, leading to the loss of open habitats. In this paper, dynamics of natural and semi-natural areas in the summit areas of the Abruzzo, Lazio e Molise National Park are investigated, highlighting changes that occurred from 1954 to present days. Historic Land cover maps have been produced by photo interpretation. A quantitative description of changes and habitats loss in relation to the socio-economic changes is provided. As expected, a forest surface expansion and an open areas decrease are observed similarly to many marginal mountains, where land abandonment and general forest/shrub recovery are the inevitable tendencies. An intense debate is still ongoing regarding the opportunity of rewilding, allowing the natural reforestation processes, versus the management of some areas, in order to preserve habitats and cultural traditional landscapes. For the EU biodiversity conservation policy to be effective, proper planning and management of interventions as well as public support and funding, become crucial when traditional activities are no longer profitable and viable for local inhabitants.

A checklist and areography of Buprestidae, Vesperidae and Cerambycidae (Coleoptera) in Abruzzo, Lazio and Molise National Park, Italy

The species of Buprestidae, Vesperidae and Cerambycidae in Abruzzo, Lazio and Molise National Park in Italy were studied based on literature data and original material. As a result, a list of 128 taxa was assembled: 49 buprestids, one vesperid and 78 cerambycids. These taxa belong to 21 areographical categories combined in seven complexes. The European complex occupies a dominant position (39.1%), followed by the Palaearctic (24.2%), Mediterranean (14.1%), Eurosiberian (10.2%), European- Iranoturanian (6.3%), Endemic (3.9%), and Cosmopolitan and Holarctic (2.3%) complexes. The Park has four endemic and one subendemic taxa.

Cost of Coexisting with a Relict Large Carnivore Population: Impact of Apennine Brown Bears, 2005-2015.

Simple SummaryEfficiently addressing human-large carnivore conflicts is a conservation issue of increasing relevance, especially in human-dominated landscapes where impact on rural economies generates negative attitudes towards large carnivores and their conservation. We quantified patterns of bear impact on farms and the costs of compensation from 2005 to 2015 in the Abruzzo Lazio and Molise National Park (central Italy), an historical stronghold of the relict and highly imperiled Apennine brown bear population, where the park authority has been adopting conflict management approaches since the 1960s. Although the compensation program is rather costly (1490 ± 589 €/bear/year), the park policy has been increasingly integrated with prevention incentives, managing to effectively avert further increases in bear damages during the study period. Concurrently, local residents generally share a positive attitude towards bears, and the number of illegally killed bears decreased in the last decade. Despite this, our findings indicate there is still room for improvement in local conflict management, and that a more efficient use of conservation funds would benefit from increased monitoring, integrated prevention, conditional compensation, and participatory processes. Lessons learned from areas of historical coexistence between humans and large carnivores provide critical insights to design successful management strategies in areas of recent and future recolonisation by large carnivores.Human-carnivore conflicts are a major conservation issue. As bears are expanding their range in Europe’s human-modified landscapes, it is increasingly important to understand, prevent, and address human-bear conflicts and evaluate mitigation strategies in areas of historical coexistence. Based on verified claims, we assessed costs, patterns, and drivers of bear damages in the relict Apennine brown bear population in the Abruzzo Lazio and Molise National Park (PNALM), central Italy. During 2005–2015, 203 ± 71 (SD) damage events were verified annually, equivalent to 75,987 ± 30,038 €/year paid for compensation. Most damages occurred in summer and fall, with livestock depredation, especially sheep and cattle calves, prevailing over other types of damages, with apiaries ranking second in costs of compensation. Transhumant livestock owners were less impacted than residential ones, and farms that adopted prevention measures loaned from the PNALM were less susceptible to bear damages. Livestock farms chronically damaged by bears represented 8 ± 3% of those annually impacted, corresponding to 24 ± 6% of compensation costs. Further improvements in the conflict mitigation policy adopted by the PNALM include integrated prevention, conditional compensation, and participatory processes. We discuss the implications of our study for Human-bear coexistence in broader contexts.

New and interesting records of jewel and longhorn beetles from Abruzzo, Lazio and Molise National Park, Italy (Coleoptera: Buprestidae and Cerambycidae)

The present article lists distributional data about 19 taxa, which are new records for the Abruzzo, Lazio and Molise National Park, respectively – 16 jewel and 3 longhorn beetles. Data about 4 Italian endemics (2 buprestids and 2 cerambycids) are listed, and the category of risk according to the Italian IUCN Red Lists is also indicated for each species (when available); among the listed species, 1 is Endangered, 1 is Near threatened and 27 are Least concern.

Correlates of parasites and pseudoparasites in wolves (Canis lupus) across continents: A comparison among Yellowstone (USA), Abruzzo (IT) and Mercantour (FR) national parks.

Little is known about the impact of infectious diseases on large carnivores. We investigated factors structuring the helminth and protozoan infections of wolves (Canis lupus) by using coprological analyses. Faecal samples (n = 342) were analysed from 11 wolf packs belonging to three different geographical and ecological settings in Italy (Abruzzo, Lazio e Molise National Park, PNALM: 4 packs, 88 samples), in France (Mercantour National Park, PNM: 4 packs, 68 samples) and in the U.S.A. (Yellowstone National Park, YNP: 3 packs, 186 samples). Parasites were found in 29.4%–88.6% of the samples and parasite taxa ranged from four to ten in each study area. Taeniidae (Taenia/Echinococcus), Sarcocystis spp. and Toxascaris leonina were most common in faecal samples from YNP, whereas Capillaria spp., Taeniidae and Uncinaria stenocephala were predominant in PNALM. We used generalised linear mixed models to assess the relationship between parasite infection or the number of parasite taxa and selected ecological drivers across study areas. Significant effects illustrated the importance of the ecological factors such as occurrence of free-ranging dogs, diet composition and wolf density, as well as the ancestry of the wolf populations, in shaping parasite-wolf communities. Additional investigations are needed to elucidate the impact of parasitic infections on wolf populations, as well as the role of anthropogenic factors in facilitating parasitic diffusion to apex predators.

Exploring the drivers of vascular plant richness at very fine spatial scale in sub-Mediterranean limestone grasslands (Central Apennines, Italy)

Secondary dry grasslands in Europe can host high levels of vascular plant richness at small spatial scales. However, in Southern Europe their biodiversity patterns are largely unexplored. In this work, we aim at: (i) estimating plant species richness patterns at very fine scales in montane dry grasslands, on limestone bedrock, in Abruzzo Lazio and Molise National Park (Central Apennines, Italy); (ii) assessing the most important physical and edaphic drivers of biodiversity patterns at multiple plot sizes. We used randomly placed nested-plot series where we measured alpha-diversity at three different plot sizes (1 m2, 0.1 m2 and 0.01 m2) and within-plot beta-diversity (as expressed by the slope of the species-area curve across plot sizes). Variable selection was performed by means of Random Forests. Relationships between selected variables and diversity measures were then assessed using Regression Trees, Linear and Generalized Linear Models. Overall, results pointed to topographically-controlled edaphic factors (soil pH and silt fraction) as the main drivers positively influencing alpha-diversity at all spatial scales, with a positive effect of rock cover and slope inclination at smaller spatial grains. Beta-diversity was positively influenced by rock cover. We suggest that high-pH, steep and/or rocky sites feature higher species richness because they lack competitive grass species. Our results are in agreement with previous works underlining the importance of less productive habitats for the conservation of secondary grassland biodiversity.

Systemic tuberculosis by MYCOBACTERIUM BOVIS in a free-ranging MARSICAN brown bear (URSUS ARCTOS MARSICANUS): a Case report

BackgroundMycobacterium bovis is known to have a wide host range and has been isolated from numerous free-ranging wildlife species, carnivores included. In bears, M. bovis has been previously reported only from a culture of pooled lymph nodes of a black bear (Ursus americanus) in the absence of lesions. The aims of this study were to describe gross and microscopic pathological findings of M. bovis tuberculosis in a deceased Marsican brown bear (Ursus arctos marsicanus).Case presentationIn March 2014, an adult female Marsican brown bear was found in the Abruzzo, Lazio and Molise National Park (Italy) showing severe non-specific clinical signs. The animal died soon after its discovery and the carcass was submitted to post-mortem examination to identify the cause of death. The bear was diagnosed with a severe Mycobacterium bovis infection, with both pathological and microbiological aspects suggesting ongoing generalization. A presumptive diagnosis of mycobacterial infection was initially made based on gross findings. Histopathology showed the presence of acid-fast bacilli in all sampled tissues along with poorly organized granulomatous lesions. Slow-growing Mycobacterium sp. was isolated from multiple organs (intestine, mesenteric lymph nodes, liver, spleen, lung and kidneys). The PCR and sequencing algorithm identified the Mycobacterium sp. isolate as M. bovis. Spoligotyping demonstrated that the M. bovis isolate belonged to spoligotype SB0120.ConclusionsThis is the first report of lethal M. bovis tuberculosis infection in a free-ranging brown bear. This pathogen could have serious adverse effects in an endangered relic population such as the Marsican brown bear. Stricter application of health regulations in force, surveillance of M. bovis infections in wild ungulates and carnivore scavengers, along with dismissal of supplementary feeding points intended for cattle or wildlife, are warranted to control the presence of bovine tuberculosis in wild and domestic animals in protected areas.

Local attitudes toward Apennine brown bears: Insights for conservation issues

Human‐carnivore coexistence is a multi‐faceted issue that requires an understanding of the diverse attitudes and perspectives of the communities living with large carnivores. To inform initiatives that encourage behaviors in line with conservation goals, we focused on assessing the two components of attitudes (i.e., feelings and beliefs), as well as norms of local communities coexisting with Apennine brown bears (Ursus arctos marsicanus) for a long time. This bear population is under serious extinction risks due to its persistently small population size, which is currently confined to the long‐established protected area of Abruzzo, Lazio and Molise National Park (PNALM) and its surrounding region in central Italy. We interviewed 1,611 residents in the PNALM to determine attitudes and values toward bears. We found that support for the bear's legal protection was widespread throughout the area, though beliefs about the benefits of conserving bears varied across geographic administrative districts. Our results showed that residents across our study areas liked bears. At the same time, areas that received more benefits from tourism were more strongly associated with positive feelings toward bears. Such findings provide useful information to improve communication efforts of conservation authorities with local communities.

Beyond home: Preliminary data on wolf extraterritorial forays and dispersal in Central Italy

Extraterritorial forays in wolves (Canis lupus) have rarely been documented, especially in human-modified landscapes of southern and central Europe. Integrating information on extraterritorial forays is currently enhanced by Global Positioning System (GPS) telemetry and contributes to our knowledge of the spatial dynamics of wolf populations. We hereby report GPS-revealed extraterritorial forays performed by 4 wolves in 3 packs in the Abruzzo, Lazio and Molise National Park (central Apennines, Italy, 2009–2010). Wolves engaged in extraterritorial forays almost exclusively during fall and winter, when they occurred on average every 22 days, for relatively brief periods (i.e., ≤4 days) and short distances (i.e., mean minimum travelled distance of 13.3 ± 7.2 SD km). Three extraterritorial forays were later revealed to be pre-dispersal movements, featuring longer duration and higher travelled distances than other extraterritorial forays and occurring 33–17 days prior to dispersal. We also anecdotally report two cases of dispersal, one involving an adult female that travelled 41.2 km to establish a new territory adjacent to her natal home range, and the other involving a yearling male that travelled a minimum of 422.2 km to the adjacent Majella National Park.

Determinants of home range size and space use patterns in a protected wolf (Canis lupus) population in the central Apennines, Italy

Large carnivores are amongst the most susceptible species to human activities, and human-modified environments pose a threat to carnivore conservation. Wolves (Canis lupus Linnaeus, 1758) in the central Apennines, Italy, have coexisted with humans since historic times and represent a good case study to assess their spatiotemporal response to anthropogenic factors. From 2008 to 2010, we investigated the spatial behavior of wolves (seven wolves in five packs and six floaters) in the Abruzzo Lazio and Molise National Park. Orographically corrected annual home ranges of resident wolf packs, estimated through the Brownian bridge movement model, averaged 104 ± 24 km2 (mean ± SD), whereas floaters used two- to fourfold larger areas (293.8–408.7 km2). We did not detect any seasonal effect on home range size, but home ranges were larger during the night and in areas of greater road density, especially during summer. By estimating core areas through an individual-based approach, we also revealed a habitat-mediated response to human presence and activity, as resident wolves preferentially established core areas at greater elevation and in the more forested and inaccessible portions of the home range.

Assessing spring frost effects on beech forests in Central Apennines from remotely-sensed data

In common beech forests the most damaging frosts are those that occur at the end of spring. At that time the fresh new leaves are at a vulnerable stage and risk to be readily killed by the freezing temperatures. The ability to identify late spring frost spatial dynamics is a key issue for understanding forest patterns and processes linked to such extreme event. The aim of this study is to detect, map and quantify the vegetation anomalies that occurred in the mono-specific beech forest of the Lazio, Abruzzo and Molise National Park (Italy) after an exceptional spring frost recorded on the 25th of April 2016. Results showed that, beech forests at lower elevations that had an early greening process were subject to spring frost damage (SFD pixels) and their productivity performance strongly decreased with respect to the previous 15 years; to the contrary the beech forests located at higher elevations did not suffer the spring frost effects (NSFD pixels) thanks to their delayed leaf unfolding phase. The duration of the effects of freezing stress for the SFD pixels was about two months, until the end of June, confirmed by Net Ecosystem Exchange measurements. This greening hiatus led to an average 14% loss of productivity compared to the previous 15 years. Elevation had a significant role on the probability of occurrence of SFD pixels. Productivity loss in SFD pixels was more severe at elevations in the range 1500–1700 m, on steeply terrains and North aspects. This study represents a step forward the systematic use of automated techniques to study areas subject to stress or anomalies from multitemporal satellite imagery and to identify break points and recovery of the greening process.

Brown bears in Central Italy: a 15-year study on bear occurrence

The Central Apennine brown bear population, that has been ascribed to the subspecies Ursus arctos marsicanus Altobello, 1921 and is endemic to Central Italy, actually survives as an isolated, small population potentially at high risk of extinction. The population of U. a. marsicanus is mostly located in Abruzzo, Lazio and Molise National Park (Central Apennines). However, the Central Apennine peripheral distribution area of this subspecies is less known and it is presumed that bear presence occurs at lower density. The purpose of this study is to provide a new contribution to the knowledge of bear presence in this peripheral area, in order to enable its protection in the Central Apennines. The monitoring of bear presence was carried out in Sirente Velino Natural Regional Park and surrounding areas (600 km2) from January 2000 to December 2014. Monitoring was conducted by collecting data along opportunistic survey trails in areas where the presence of bears had been previously confirmed by direct observations. A total of 237 records of bear presence were collected during the study period. Distribution of historic bear data reported for the period 1900–1991 for the study area (n = 61) and present data are compared. Data show a continuous presence of bears in the study area during the last 15 years. The monitoring program has also provided evidence of the stable presence of wintering bears and a more or less continuous presence of breeding bears in the study area, although observations of females with cubs were not available every year. Results led us to speculate about a wider than previously expected stable presence of brown bears in the peripheral Apennine range. These peripheral areas of bear distribution, both inside and outside protected areas, are crucial for conservation and more effort must be made to estimate bear occurrence outside the core range.

Seasonal and annual variation in the food habits of Apennine brown bears, central Italy

Abstract Only scanty and outdated knowledge is available on the food habits of the Apennine brown bear (Ursus arctos marsicanus) population, despite its critical conservation status. Based on 2,359 scats, collected from June 2006 through December 2009, we documented seasonal and annual variation in the diet of this bear population within its 1,294-km2 core distribution in Abruzzo, Lazio, and Molise National Park and its external buffer area in central Italy. Using correction factors to estimate digestible energy, we revealed substantial consumption of plant matter by bears, including herbaceous vegetation in spring (mean ± SD; 31.7% ± 25%) and early summer (19.0% ± 7%), a variety of naturally occurring berries in summer (56.5% ± 14%), and hard mast (66.9% ± 21%), largely supplemented by fleshy fruits (26.3% ± 18%), in the fall. Bears also consumed insects, mostly ants, in early summer (38.3% ± 7%), and wild ungulates in spring (10.2% ± 11%). Hard mast production strongly influenced year-to-year variation ...

Comparative survey of canine parvovirus, canine distemper virus and canine enteric coronavirus infection in free-ranging wolves of central Italy and south-eastern France.

Diseases likely affect large carnivore demography and can hinder conservation efforts. We considered three highly contagious viruses that infect a wide range of domestic and wild mammals: canine parvovirus type 2 (CPV-2), canine distemper virus (CDV) and canine enteric coronaviruses (CECoV). Infection by either one of these viruses can affect populations through increased mortality and/or decreased general health. We investigated infection in the wolf populations of Abruzzo, Lazio e Molise National Park (PNALM), Italy, and of Mercantour National Park (PNM), France. Faecal samples were collected during one winter, from October to March, from four packs in PNALM (n = 79) and from four packs in PNM (n = 66). We screened samples for specific sequences of viral nucleic acids. To our knowledge, our study is the first documented report of CECoV infection in wolves outside Alaska, and of the large-scale occurrence of CPV-2 in European wolf populations. The results suggest that CPV-2 is enzootic in the population of PNALM, but not in PNM and that CECoV is episodic in both areas. We did not detect CDV. Our findings suggest that density and spatial distribution of susceptible hosts, in particular free-ranging dogs, can be important factors influencing infections in wolves. This comparative study is an important step in evaluating the nature of possible disease threats in the studied wolf populations. Recent emergence of new viral strains in Europe additionally strengthens the need for proactive monitoring of wolves and other susceptible sympatric species for viral threats and other impairing infections.

Unexpected consequences of reintroductions: competition between reintroduced red deer and Apennine chamois

Reintroductions are commonly used to restore the local biological diversity and/or save threatened taxa. In human-altered landscapes, we may expect that reintroduced species affect taxa already present. In Abruzzo, Lazio and Molise National Park (central Apennines, Italy), a 30% decline in the abundance of ‘vulnerable’ Apennine chamois (2005: c. 650 individuals, 2010: c. 450 individuals) has been recorded, whereas red deer (reintroduced in 1972–1987: 81 individuals) have greatly increased (2010: > 2500 individuals). We investigated space and diet overlaps between red deer and Apennine chamois, and their effects on foraging behaviour of the latter. We also compared the composition of grasslands with that recorded when the former were absent. In 2010–2011, we found out: (1) a great space (> 75%) and diet (> 90%) overlap between deer and chamois; (2) a significant increase of unpalatable plant species and a decreasing trend of the nutritious, most grazed species by chamois, in respect to when deer were absent; (3) irrespective from vegetation type, a significantly reduced bite rate of adult female chamois in patches used also by deer, compared with areas without deer. Our results suggest a negative effect of red deer on the availability of nutritious plant species in summer–autumn, possibly because of grazing and physical damage on the grassland caused by trampling. Environmental conditions and access to high-quality forage in the warm season influence the winter survival of offspring of mountain ungulates. Our results indicate that interspecific overlap in resource use with an increasing, reintroduced population can threaten rare taxa. Reintroductions of potentially competing species should be avoided in areas where populations of threatened taxa exist.

Detection of an East European wolf haplotype puzzles mitochondrial DNA monomorphism of the Italian wolf population

Southern European wolves suffered from reiterated population declines during glacial periods and historically due to human persecution. Differently from other European wolf populations, a single mitochondrial DNA (mtDNA) control region haplotype (W14) has been so far described in the Italian wolves, although no intensive genetic sampling has ever been conducted in historical source populations from central and southern Italy. Using non-invasive genetic techniques, we report the occurrence of an unexpected mtDNA haplotype (W16) in the wolf population of the Abruzzo, Lazio and Molise National Park (PNALM), central Italy. This haplotype, detected in three out of 90 faecal samples from the PNALM, was previously reported in wolves from the North Carpathians, Slovakia and the Balkans only. Microsatellite analysis and molecular sex determination confirmed that the W16 samples belonged to three distinct wolves. Although alternative explanations can be formulated for the origin of this mtDNA haplotype in the otherwise monomorphic Italian wolf population, assignment procedures indicated the likely admixed ancestry of one W16 sample with East European wolves. Anthropogenic introgression with dogs has been detected in the Italian wolf population using nuclear DNA microsatellites, but no population-wide genetic survey had previously reported a mtDNA control region variant in Italian wolves. Our findings strongly suggest that, in addition to wolf×dog hybridization, captive-released wolves or wolf×dog hybrids may successfully interbreed with wolves in the wild, and that human-mediated introgression may occur even in well established protected areas.