Mold Formation Research Articles

Avoid Bruising of Tomatoes in Short Time

Cooling aluminum chambers have emerged as a promising solution for the storage of tomatoes, addressing the critical need to extend their shelf life while preserving quality post-harvest. This study delves into the efficacy of utilizing these chambers, aiming to optimize storage conditions for maximum preservation benefits. Through meticulous experimentation, various parameters such as temperature, humidity, and airflow dynamics within the chamber were meticulously examined. Additionally, the study scrutinized the influence of different packaging methods on tomato preservation efficacy. The findings underscore the remarkable capability of cooling aluminum chambers to regulate storage environments, effectively retard ripening processes and mitigating microbial proliferation. Lower temperatures within the chambers significantly curtailed the pace of tomato ripening, thus extending their shelf life appreciably. Moreover, meticulous humidity control within the chambers prevented moisture loss, curbing mold formation and decay. Furthermore, the study elucidated the profound impact of packaging methods on ethylene production and gas exchange, thereby influencing tomato quality during storage.

The effect of lemon juice addition and cold storage on the total phenolic content and antioxidant activity of cold mate tea

Although mate tea, made from the infusion of the Ilex paraguariensis plant, is typically consumed cold, it should be properly prepared, stored, and consumed within the appropriate time frame to fully appreciate the tea’s aroma and components. This study aimed to investigate the impact of adding lemon juice to mate tea and cold storage on the total phenolic content, antioxidant activity, and total mold and yeast count of the tea. It was hypothesized that the addition of lemon juice would increase the total phenolic content and antioxidant activity and extend shelf life. At the start of storage, the sample with 2% (v/v) lemon juice added showed the highest total phenolic content (14.06 ± 0.05 mg GAE/100 mL). The antioxidant scavenging activity of the tea samples was investigated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. While all the studied sample showed higher DPPH inhibition than the control group, the highest value was found for tea sample with 4% lemon addition on 3rd day (97.618 ± 1.429). No mold or yeast growth was observed in the control group and the samples with 2% and 4% lemon juice addition up to day 21. In the sensory evaluation, the tea that contained 4% lemon juice was the most preferred. In conclusion, if mate tea is prepared and stored appropriately, it can maintain its antioxidant capacity for an extended period without the formation of harmful molds or yeasts and any deterioration in its sensory properties.

Insight into the Effect of Methylglyoxal on the Conformation, Function, and Aggregation Propensity of α-Synuclein.

It is well-known that people suffering from hyperglycemia have a higher propensity to develop Parkinson's disease (PD). One of the most plausible mechanisms linking these two pathologies is the glycation of neuronal proteins and the pathological consequences of it. α-Synuclein, a key component in PD, can be glycated at its fifteen lysine. In fact, the end products of this process have been detected on aggregated α-synuclein isolated from in vivo. However, the consequences of glycation are not entirely clear, which are of crucial importance to understand the mechanism underlying the connection between diabetes and PD. To better clarify this, we have here examined how methylglyoxal (the most important carbonyl compound found in the cytoplasm) affects the conformation and aggregation propensity of α-synuclein, as well as its ability to cluster and fuse synaptic-like vesicles. The obtained data prove that methylglyoxal induces the Lys-Lys crosslinking through the formation of MOLD. However, this does not have a remarkable effect on the averaged conformational ensemble of α-synuclein, although it completely depletes its native propensity to form soluble oligomers and insoluble amyloid fibrils. Moreover, methylglyoxal has a disrupting effect on the ability of α-synuclein to bind, cluster and fusion synaptic-like vesicles.

Analysis of the technology of manufacturing a semi-finished part for a hollow part of a complex geometric form using the technology of hot volume stamping

Abstract. Background. Today, the actual issues in the production of metal products are the saving of materials and the productivity of production, as this has a significant impact on the cost of finished products. This especially applies to hollow products with a complex geometric shape. Such products include parts or semi-finished products that have hollow cavities and additional elements in the design in the form of flanges, or variable wall thickness along the height of the product. Such parts are widely distributed in mechanical engineering, aircraft construction and in the production of special purpose products, in particular in the field of ammunition production. Therefore, the manufacturing technology of such parts should ensure mass production and have an economic effect. Goal. By calculation, using the finite element method (FEM) in the DEFORM software environment, determine the option of stamping, from blanks of different diameters, a semi-finished product of a hollow part of a complex geometric shape using the technology of hot three-dimensional stamping, and analyze the proposed technological process of obtaining a semi-finished product. Methodology of implementation. Using MSE in the DEFORM software environment, simulation of hot three-dimensional stamping of a semi-finished product was carried out for a hollow part of a complex geometric shape made of blanks of different diameters. In this way, the option of obtaining a semi-finished product with a minimum number of transitions and a guaranteed possibility of implementing the technological process of mold formation was chosen. For the selected option, an analysis of the force modes of forming, normal stresses on the contact surfaces of the workpiece with the tool, and the stress-strain state of the deformed metal was performed. The results. A semi-finished product manufacturing technology for a hollow part of a complex geometric shape using hot three-dimensional stamping technology is proposed. This technology will be implemented in the production of the adapter housing part, which is used in the design of the 120 mm mine. By calculation, with the help of MSE in the DEFORM software environment, the variant of semi-finished product molding for the "Body-adapter" part was determined using the technology of hot three-dimensional stamping, and an analysis of the technological operation of semi-finished product molding was carried out. The temperature regime of the process, technological efforts, parameters of the stress-strain state of the deformed material, the distribution of normal stresses on the deforming tool are determined. The results of the conducted computer modeling make it possible to take into account the design features and select the necessary technological equipment for mass production. Conclusions. The technology for serial production of semi-finished products by hot volumetric stamping has been developed and substantiated. This technology made it possible to increase the rate of material utilization by two times compared to the technology of obtaining a part by mechanical processing from graded rolled steel.

Film Formation of Iodinated Latex Dispersions and Its Role in Their Antimicrobial Activity.

Waterborne coatings with intrinsic antibacterial attributes have attracted significant attention due to their potential in mitigating microbial contamination while simultaneously addressing the environmental drawbacks of their solvent-based counterparts. Typically, antimicrobial coatings are designed to resist and eliminate microbial threats, encompassing challenges such as biofilm formation, fungal contamination, and proliferation of black mold. Iodine, when solubilized using ethylene glycol and incorporated as a complex into waterborne latex dispersions, has shown remarkable antimicrobial activity. Here, we demonstrate the effect of the film formation process of these iodinated latex dispersions on their antimicrobial properties. The effect of iodine on the surface morphology and mechanical, adhesion, and antimicrobial properties of the generated films was investigated. Complete integration and uniform distribution of iodine in the films were confirmed through UV-vis spectrophotometry and a laser Raman imaging system (LRIS). In terms of properties, iodinated films showed improved mechanical strength and adhesion compared with blank films. Further, the presence of iodine rendered the films rougher, making them susceptible to bacterial adhesion, but interestingly provided enhanced antibiofilm activity. Moreover, thicker films had a lower surface roughness and reduced biofilm growth. These observations are elucidated through the complex interplay among film thickness, surface morphology, and iodine properties. The insights into the interlink between the film formation process and antimicrobial properties of iodinated latex dispersions will facilitate their enhanced application as sustainable alternatives to solvent-based coatings.

Resistance to Cassava Whitefly (Bemisia tabaci) among Eastern and Southern African Elite Cassava Genotypes.

Cassava whitefly, Bemisia tabaci, directly damages cassava leaves by feeding on phloem, causing chlorosis and abscission, leading to a yield loss of up to 50%. The pest also causes indirect damage through sooty mold formation. Most Ugandan cassava varieties resist cassava mosaic disease (CMD) and tolerate cassava brown streak disease (CBSD), but little is known about their response to whitefly infestation. The main objective of this study was to identify cassava genotypes with putative resistance to whitefly in Uganda. This was conducted on 24 improved cassava varieties in three agro-ecological zones during the second rains of 2016. Monthly data were taken for adult and nymph counts, whitefly and sooty mold damage, and CMD and CBSD severities from 2 to 9 months after planting (MAPs). The results show that the whitefly population is highly significantly (p < 0.000) amongst varieties across the three agro-ecological zones. Mkumba consistently supported the low adult numbers and nymphs. The findings demonstrate the potential of the improved cassava varieties as sources of whitefly resistance for sustainable management.

Predicting early mycotoxin contamination in stored wheat using machine learning

With continued global population growth and current rate of climate change, grain loss during storage remains a major contributor to postharvest losses of wheat (Triticum aestivum L.). Infection with mycotoxin leads to degradation or even discarding of stored grain, causing economic losses and risks to food security. Deep learning models have been used in the agricultural domain for detecting prevalent diseases or contamination; however, data scarcity remains a critical bottleneck for rapid implementation of computer vision in this field. Herein, a compact convolutional transformer (CCT)–based model was applied to classify contaminated wheat by deoxynivalenol (DON) and aflatoxins (AFB1, AFB2, AFG1, and AFG2), which was divided into three main classes: healthy, incipient, and contaminated. The classification was performed based on elevated CO2 respiration rate (≥31.20 ± 0.62 mg CO2 kg−1 h−1) and visual appearance of mold formation in initial and severe stage since the start the storage experiment. The proposed CCT model achieved an accuracy of 83.33%, with the contaminated class demonstrating the highest performance metrics, including precision (1.0), recall (0.90), and F1-score (0.95), followed by the healthy and incipient classes. At the same time, explicit classification between the healthy and incipient classes deserves further improvement because it is highly relevant for the timely detection of spoilage and prevention of proliferation of mycotoxins in stored wheat.

An environmentally friendly multifunctional soy protein adhesive composed of rosin acid derivative inspired from organic-inorganic hybrid structure

Soy protein adhesives have the advantages of low price and renewability but are limited by terrible bonding strength and poor water resistance, which restricts the practical application. Herein, inspired by the structure of mussels and organic-inorganic hybrid strengthening mechanism, a novel and bio-based soy protein adhesive (SPI-RA-CSA) based on rosin acid derivative and calcium sulfoaluminate was generated. According to the optimization of response surface methodology (RSM), the best proportion of adhesive SPI-RA6-CSA1 was chosen. The dry and wet shear strengths of SPI-RA6-CSA1 adhesive reached 1.92 and 1.35 MPa, respectively, which were 120% and 210% higher than original soy protein adhesive. Meanwhile, SPI-RA6-CSA1 adhesive showed excellent coating performance even if it was coated on wet wood veneer. In addition, SPI-RA6-CSA1 adhesive effectively inhibited the formation of mold and exhibited outstanding antimicrobial ability for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) with antimicrobial rates of 87.36% and 85.73%, respectively. What's more, SPI-RA6-CSA1 adhesive demonstrated prominent flame retardant properties reducing the harm of fire accidents. SPI-RA6-CSA1 adhesive also possessed the characteristics of low energy consumption and environmentally friendliness by Life Cycle Assessment (LCA). Therefore, this simple strategy provides workable guidance for the production of multifunctional and green soy protein adhesive.

Research on the forming and demolding process of shape memory self-demolding mold

The molding method is highly anticipated in engineering fields and has been preliminarily applied. However, traditional demolding methods introduce subtle deviations in demolding direction, leading to demolding defects and reduced forming accuracy. To address this, we developed an innovative Shape Memory Polymer (SMP) mold. Through finite element simulation, we extensively investigated the effects of diaminodiphenylmethane (DDM) addition, temperature, and load on the template forming and shape recovery processes. Finally, we experimentally verified the feasibility of the self-demolding of the template. The findings demonstrate that as the amount of DDM added increases, the glass transition temperature of SMP progressively elevates. This can be attributed to the enhanced crosslinking triggered by the addition of DDM. Consequently, during the stage of preserving shape in mold formation, SMP exhibits a heightened storage modulus and diminished shape recovery. Notably, when 1.5 g of DDM is added, the shape memory template exhibits the least shape recovery, with a shape retention rate of 78.2%. Conversely, employing 1.6 g of DDM generates the highest shape recovery but only achieves a shape retention rate of 59.5%. Augmenting the amount of DDM is advantageous in accomplishing template demolding. Higher temperatures expedite the initiation of the shape recovery process, facilitating template demolding during the shape recovery stage. Moreover, increasing the load can minimize template rebound and enhance the precision of mold formation. However, it also intensifies the complexity of self-demolding. Consequently, it is imperative to sensibly determine and implement suitable process parameters in applications.

STUDY OF BIOLOGICALLY ACTIVE POLYMER HYDROGELS FOR REGULATION OF WATER-LIPID BALANCE

The article shows studies on the study of biologically active polymeric hydrogel materials for the regulation of water-lipid balance. It is shown that the current trend in the development of biologically active polymers and materials based on them is the technology of creating effective systems for the transdermal delivery of drugs and active substances to the human body. As part of the literature review, it has been proven that transdermal delivery systems based on biologically active polymeric materials are of increased interest in the administration of drugs through the skin, for local therapeutic effects on the affected skin during systemic local drug delivery, and they are also widely used in the direction biologically active materials in the form of various types of polymeric hydrogels. In the article, a study was conducted to determine the features of obtaining biologically active polymeric hydrogel materials for regulating the water-lipid balance. Food gelatin TM "Mriya" (Ukraine), glycerin, sodium alginate, humic acids and distilled water were used in the work. The development and research of biologically active polymeric hydrogel materials for the regulation of water-lipid balance has been carried out. It has been established that humic acids in polymer hydrogels based on gelatin and sodium alginate have a high antibacterial activity and completely stop the processes of mold formation in them. It has been proven that compositions based on gelatin and sodium alginate with a content of humic acids of 15 % are the most effective in terms of obtaining hydrogels with an antibacterial effect. Effective biologically active polymeric hydrogel materials based on gelatin and sodium alginate, modified with different content of humic acids, have been obtained and studied. Modification of biopolymer hydrogels based on gelatin and sodium alginate with humic acids makes it possible to obtain biologically active polymeric hydrogel materials with an increased degree of swelling and the ability to significantly improve the moisture-lipid balance of the skin: according to the initial indicators of moisture 34–36 % and fat content 8–10 % of the skin, it increases them up to 58–66 % and 52–60 % respectively. In fact, thanks to the use of biologically active polymeric hydrogel materials based on gelatin and sodium alginate modified with humic acids, it becomes possible to transform the state from weakly oily tough to highly moist oily elastic skin. The effect of improving the moisture-lipid balance of the skin is enhanced along with the content of humic substances.

Development and Characterisation of New Hydrogels for Medical Science Education

Organ models that resemble the original anatomy are needed in the education and training of human and veterinary doctors. The organs should be as close as possible to the original in shape, colour and feel. Furthermore, these organ models must be able to be handled in such a way that they can be examined by palpation and imaging (ultrasound, X-ray, CT and endoscopy). The diagnostic procedures should detect pathologies such as cysts, tumours, ruptures, haemorrhages or air collections. Following the diagnostic measures, it must be possible to initiate a therapy. Possible therapeutic measures are surgery with a scalpel, electrosurgery or puncture. The present work deals with the production of training models by means of a casting process. With this method, the models can be produced quickly and at low cost. Different compositions of hydrogels and other biomaterials are investigated. These gels are examined for the following properties, among others: thermostability, electrical conductivity and dimensional stability under pressure. Other parameters such as pot life, cross-linking time and durability in relation to mould formation will also be considered.

Anti-angiogenic and oxidative effects of sodium benzoate at different concentrations in chorioallantoic membrane model

&lt;b&gt;Aim:&lt;/b&gt; Due to the increased consumption of packaged foods, exposure to food additives is also increasing. Sodium benzoate (SB), a frequently used food additive, is generally used in alcoholic beverages, fruit, and vegetable juices, carbonated soft drinks, canned food, and various sauces. It is used to inhibit the formation of mold, yeast, and bacteria. This study was carried out to investigate the effects of SB exposure on angiogenesis and oxidant-antioxidant balance.&lt;br /&gt; &lt;b&gt;Materials &amp;amp; methods:&lt;/b&gt; Three different concentrations of SB, bevacizumab, and empty pellets were prepared, placed on chorioallantoic membrane (CAM), and examined for anti-angiogenesis. Total antioxidant capacity (TAC) and total oxidant capacity (TOC) measurements were made in the albumen samples, and oxidative stress index (OSI) value was calculated.&lt;br /&gt; &lt;b&gt;Results:&lt;/b&gt; The control group had no anti-angiogenic effect, but the bevacizumab group had a strong anti-angiogenic effect.10&lt;sup&gt;-3&lt;/sup&gt; M SB had a weak anti-angiogenic effect, but 10&lt;sup&gt;-4&lt;/sup&gt; M SB and 10&lt;sup&gt;-5&lt;/sup&gt; M SB showed no anti-angiogenic effect. TOC levels increased with SB in a dose-dependent manner. TAC levels decreased depending on the dose in the experimental groups with SB application. OSI levels increased depending on the dose increase in SB.&lt;br /&gt; &lt;b&gt;Conclusions:&lt;/b&gt; SB exposure caused a dose-dependent increase in oxidative stress and anti-angiogenic effect in CAM model.

LDPE-Natural Rubber Composite Film as Active Packaging: A Paradigm Shift in Oxygen Scavengers.

Oxygen scavenging films, an emerging type of active packaging, play a crucial role in preserving the freshness and quality of food products. In this study, we proposed an extruded film made of low-density polyethylene (LDPE) with 5% natural rubber (NR) as the oxygen scavenging film. Characterization of the film revealed that its morphological and barrier properties remained intact, while the elongation attribute was enhanced. The obtained film was standardized for activation scavenging kinetics by varying the UV dose (time and distance). At the optimal UV dose, the film exhibited a total scavenging capacity of 61 cc/g. To assess the film's functionality, FTIR spectra were analyzed before and after exposure to oxygen, confirming the film's ability to scavenge oxygen based on observed peaks at 1718 and 3425 cm-1. Considering that bread and khoa (fatty food) are sensitive to oxygen, they were selected for testing the application of the oxygen scavenging film. Sensory analysis of bread samples, including appearance and mold formation, as well as microbial load studies, indicated that the shelf life of bread increased from 2-3 days (control) to 4-5 days when packed in the NR-based film and stored at 27 ± 2 °C. Similarly, when applied to khoa, the film extended its shelf life by 3 days compared to the control while maintaining sensory attributes and preserving nutritional value, such as fatty acids. In general, the developed oxygen scavenging film effectively prevents the detrimental effects of oxygen on food products, leading to an extension of their shelf life. This has significant implications for the food industry, as it helps mitigate the negative consequences of oxygen exposure and enhances the product shelf life.

The Influence of Injection Temperature and Pressure on Pattern Wax Fluidity

In the investment casting process, the pattern made of wax is obtained in a die for further formation of a shell mold. The problem of die-filling by pattern wax is significant because it influences the quality of the final casting. This work investigates three commercial pattern waxes’ fluidity with a newly developed injection fluidity test. It was shown that the fluidity of waxes increased with increasing injection temperature and pressure, and the simultaneous increase in temperature and pressure gives a much more significant enhancement of fluidity than an increase in temperature or pressure separately. The rheological behavior of the waxes was also investigated at different temperatures using a rotational viscosimeter, and temperature dependences of waxes’ dynamic viscosity were determined. It was shown that wax viscosity is increased more than ten times with decreasing temperature from 90 to 60 °C. A good correlation between wax fluidity and its viscosity is observed, which is different from metallic alloys, where the solidification behavior is more critical. The difference in wax flow behavior in comparison with metallic melts is associated with the difference in dynamic viscosity, which for investigated waxes and metallic melts is 3000–27,000 mPa·s and 0.5–6.5 mPa·s, respectively. The difference in investigated filled waxes’ fluidity is observed, which can be associated with the type and amount of filler. The twice-increasing fraction of cross-linked polystyrene decreases fluidity twice. At the same time, terephthalic acid has a minor influence on wax fluidity.

Development of technology for the production of useful jelly candies from cucurbit crops on a natural basis

The object of the study are the fruits of cucurbits crops, as well as confectionery products based on the fruits of watermelons and melons. The fruits of watermelons and melons are not processed on an industrial scale for a number of reasons: the lack of technological solutions for primary processing and preparation for processing, the instability of raw materials for processing. The pulp of watermelons must be processed within 5 hours under certain conditions; in the pulp of melons, changes in microbiological indicators begin within an hour of storage at room conditions. Because fruits have a high moisture content, the solution for effective fruit processing is to bind this moisture with thickeners and produce jelly candies. Sweets made without antioxidants did not withstand the standard shelf life for jelly sweets, and the growth of mold fungi increased. The reason may be the low acidity of the raw material. Citric acid was added as an antioxidant. There were problems of taste and smell; citric acid interrupts the natural smell of raw materials. During the search, the powder of dried, red-fruited rowan was chosen. The finished product had a barely perceptible natural aroma, the powder did not affect the taste. There were inclusions in the cross-section, but the appearance became more attractive. Jelly sweets without the addition of rowan powder had active mold growth on the 7th day of storage at a temperature of 22–25 °C; with the addition of rowan powder under the same storage conditions, they showed growth on the 16th day. The finished product meets all physical, chemical, and microbiological standards. The shelf life of such jelly sweets at a temperature of +6 °C in sealed packaging is up to 30 days without mold formation

The Heliconoides Modified Dissolution Index-HMDI: Do methane seepage environments affect the preservation state of Heliconoides inflatus?

Pteropods are holoplanktonic molluscs presently endangered because their fragile aragonitic shell is very sensitive to ocean acidification. The preservation of pteropods, and in particular of the mesopelagic Heliconoides (Limacina) inflatus, has been used to assess aragonite saturation state in Quaternary sediments (Limacina Dissolution Index, LDX) as an inferred proxy for climatic changes.Three deep-sea cores retrieved in 2016 during the EUROFLEETS2 SEMSEEP cruise offshore Israel are investigated to assess the preservation degree of H. inflatus (d'Orbigny, 1835) in representative environments across the base of the southeastern Mediterranean margin (cold water coral, active seepage pockmark and deep sea channel areas). In some core intervals, yellowish, recrystallized internal molds of this species are present. They have been previously observed in the Eastern Mediterranean, but the nature and origin of their colour was never explained. Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Electron Backscatter Diffraction (EBSD) revealed that the recrystallized molds of the H. inflatus shells are formed by aragonite needles and high-Mg calcite crystals. The formation and preservation of these internal molds is induced by advective methane emissions, typically precipitating aragonite and High-Mg calcite phases close to the sediment–water interface. These molds were never used in previous applications of the LDX or in any existing ranking of pteropod preservation. Therefore, we have categorized these peculiar features combining previous pteropod ranking descriptions with the new observations to develop a modified biotic index (Heliconoides Modified Dissolution Index-HMDI).High values of the HMDI at the base of the pockmark core correspond to the interval where also active seepage-related features – e.g., bubble emissions, low-oxygen benthic foraminifera, chemosymbiotic molluscs, small fragments of authigenic carbonate crusts and slightly depleted δ13C values – occur, showing the applicability of the HMDI to methane seeps environments.

Moisture- and mould-resistance: multi-modal modelling leveraging X-ray tomography in edge-sealed cross-laminated timber

Edge-sealing, which involves treating the edges of wood products, improves water resistance. This study investigated the feasibility of edge-sealed cross-laminated timber (CLT) panels to reduce capillary water uptake, thereby resisting mould formation. The water and vapour permeabilities of ten characteristically different single-layer sealant coating systems were systematically determined. Multi-modal assessment leveraged by computed tomography (CT) scanning methodology was used to enhance detection of material characteristics beyond the standard coating permeability assessment. Moisture content was observed to change during the specimens’ absorption and desorption depending on the sealant system applied. The results revealed different characteristics of coatings during the water absorption and desorption stages. Findings from this study were used to develop recommendations regarding the water resistance of coating systems, curing time, susceptibility to mould formation, and industrial applicability. Results suggest that edge-sealed CLT could minimise the risk of mould formation, which can occur at worksites with minimal weather protection. The method developed in this study provides a basis to evaluate new coating systems and determine which use case is the best for a particular coating type. This study also incorporates insights from industry to identify future research orientations, which may pave the way for new designs and assessment techniques.

Practical Use of Damage Functions for Environmental Preventive Conservation and Sustainability—Examples from Naturally Ventilated Buildings

This work explores the potential of using damage functions to assess cultural heritage environments. Changes caused by dimensional variation due to fluctuations in relative humidity are assessed using two accessible functions, and a third is discussed. The risk of mould growth is assessed from a time series of temperature and RH data. The results of previous studies comparing predictions from four functions to observed mould formation are reviewed, and the practical aspects of using the functions are described. Two situations related to metal and stone risk are described, comparing environments for display and assessing new or refitted buildings for storage. The use of functions to improve sustainability and their combination with performance models to predict carbon footprints are discussed.

Влияние препарата «Биостим» на рост и развитие семян и сеянцев древесных видов в засушливой зоне

The aim of the study was to study the effect of the biostimulator of growth «Biostim» on the germination and development of seeds and seedlings of woody species in order to develop a resource-saving technology of cultivation for nursery breeding in the Volgograd region. The objects of research were the woody species Quercusrobur L., Robiniapseudoacacia L., PopulusbolleanaLouche., Populusnigra var. italica Münchh., Catalpa bignonioides, Pinusnigra subsp. pallasiana (Lamb.), Platycladusorientalis (L.) Franco., Piceapungens Engelm. ofdifferentgeographicalorigin. As a result of the conducted studies, the best result was shown by poplars and catalpa with a concentration of «Biostim» at a dose of 2.5 l/ha (20 ml) for cuttings and 20 ml/l for seeds. The concentration of the preparation at a dose of 10 ml / kg (the consumption of the working solution is 1.5-2 l/kg) with soaking of seeds before sowing for 10-20 minutes, followed by drying, was suitable for Robiniapseudoacacia. The concentration of the preparation in a dose of 10 ml / l with soaking of the seeds before sowing for 10-20 minutes followed by drying was suitable for robiniapseudoactation. The worst result in the test of laboratory germination of seeds was shown by oak petiolate, due to the lack of reaction of seeds to processing and their mold formation. In coniferous species, the best result was shown by Crimean pine with a concentration of the preparation«Biostim» of 10 ml / l and 2.5 l / ha. The germination of spruce and thuja seeds was weak. According to the results of the test of the preparation «Biostim» on seedlings and seeds in a nursery on dark chestnut soils, the best result was shown by seedlings of poplar and catalpa species with a concentration of «Biostim» at a dose of 2.5 l/ha and 20 ml/l for seeds. Additional top dressing with poplar preparation proved to be effective, after which good growth, development and leafing of plants were observed. Seedlings and seeds of eastern thuja and blue spruce after additional processing grew and developed satisfactorily, subsequently their drying and loss was observed, which makes the topic of testing biostimulators of growth on conifers is promising for further research.

Histopathological Inspection of the Occurrence of Lung Adenocarcinoma in Sheep in Tehran Province

Sheep pulmonary adenomatosis is a chronic respiratory disease with a long incubation period that many countries have long recognized. This disease is specific to sheep and most prominent in sheep over three years old. The cause of this disease in sheep is Beta Retroviruses (JSRV) which are transmissible among sheep. Since 1342, there have been reports of clinical cases of the disease in different regions of Iran. So far, histopathology has been one of the best diagnostic methods for this disease. The conducted research inspects the occurrence rate and pathological lesions of this disease in a slaughterhouse located in Tehran. All the studied sheep in this research were bred in the livestock farms of Tehran province and referred to the slaughterhouse. During routine inspections, the lungs that showed different macroscopic signs of pneumonia had been primarily identified and recorded; later, more thorough evaluations of macroscopic and microscopic examinations were performed. One hundred sheep lungs, which showed visible lesions of interstitial pneumonia, were selected for histopathological research. These lungs were generally heavy, edematous, had no signs of collapse, showed rib marks on the lung parenchyma, and appeared waterlogged in some cases. The affected areas were firm and light grey in some lungs. OPA histopathological lesions were observed and documented in 25 out of 300 suspicious lung samples. All twenty-five of the damaged lungs were almost similar in histopathological appearance and showed papillary protrusions consisting of short cuboid to columnar cells inside the ducts of alveoli and bronchioles, respectively. In the atypical form of the disease, which included all cases observed in the present study, infiltration of mononuclear cells and connective tissue was more substantial; large and foamy macrophages were also observed in the alveoli and bronchioles that were present in the vicinity of neoplastic lesions. The classical form of OPA, characterized by the infiltration of macrophages and lymphocytes and the formation of neutrophilic and fibrin molds, was observed in any of the cases.