Modified Ziehl-Neelsen Technique Research Articles

Detection of <I>Giardia</I> cyst and <I>Taenia</I> eggs in river water samples collected from Aleto River in Eleme Local Government Area, Rivers State, Nigeria

This study aimed at detecting Giardia cyst and Taenia eggs in river water samples collected from Aleto River in Eleme Local Government Area, River State, Nigeria, to check for potability and purity of water.Water samples of river water were collected from two points (downstream and midstream) of Aleto river. Labelled clean sample bottles with tightly-fitted lids were used to collect water samples from the river and stored in coolers with ice packs to maintain their temperature and transportation to the laboratory. Collected water samples were transferred into sterile vial tubes and centrifuged at 2000 rpm for five minutes, after which the supernatant fluids were discarded, while the sediments smeared using spatula on labelled slides stained with specific dyes, using the wet mounts, trichome stain technique, and modified Ziehl–Neelsen techniques, and viewed under the light microscope to identify the presence of parasites.This study showed the presence of ten trophozoites of Giardia lamblia and ten eggs of Taenia species isolated from the river water samples collected. These findings highlight the potential health risks associated with waterborne parasites and emphasizes the importance of effective water quality management to safeguard public health. Furthermore, the observation of parasites underscores the need for comprehensive water quality assessment and monitoring. This study highlights the contamination of the river with parasitic organisms and urgently needs effective water management strategies to mitigate the risks to public health. Vital interventions encompass enhancing sanitation practices, curbing fecal contamination, and augmenting water quality surveillance mechanisms.

Intestinal parasitic infections and predisposing factors among prison inmates in Southern Ghana: A cross-sectional study.

The environment within prisons, such as overcrowding, lack of access to portable water, poor sanitation, and hygiene predisposes inmates to infections, including intestinal parasitic infections (IPIs). This study therefore determined the prevalence and associated factors of IPIs among prison inmates in Southern Ghana. A cross-sectional study using the stratified sampling technique was employed. The study recruited 461 prison inmates across three notable prisons, with 50 inmates from Ho Central, 357 inmates from Nsawam Medium Security, and 54 inmates from Sekondi Central Prisons all in Southern Ghana. A structured closed-ended questionnaire was administered to collect data on sociodemographics, lifestyle/behavioral characteristics, and signs and symptoms of IPIs. In addition, stool samples were collected and analyzed for the presence of various stages of intestinal parasites (trophozoites, cysts, ova, and larva) using formol-ether concentration, and the modified Ziehl-Neelsen techniques. The prevalence of IPIs among inmates of the three selected prisons was 38.2% (95% confidence interval [CI]: 33.72%-42.79%). The prevalence of IPIs among inmates of the three prisons, the Ho Central, Nsawam Medium Security, and Sekondi Central were 46% (95% CI: 31.82%-60.68%), 37.5% (95% CI: 32.49%-42.79%), and 35.2% (95% CI: 22.68%-49.38%), respectively. After adjusting for confounders, the following factors; handwashing after defecation (adjusted odds ratio [AOR]: 0.05 [0.00-0.67]; p = 0.024), trimmed fingernails (AOR: 0.32 [0.13-0.76]; p = 0.011), itchy skin (AOR [95% CI]: 5.99 [3.43-10.43]; p < 0.001), anal itching (AOR [95% CI]: 0.35 [0.19-0.62]; p < 0.001), nausea (AOR [95% CI]: 5.57 [3.22-9.65]; p < 0.001), and worm expulsion (AOR [95% CI]: 3.80 [1.42-10.18]; p = 0.008) were found to be associated with intestinal parasitic infections. The study revealed that the prevalence of intestinal parasitic infections among prisons in Southern Ghana is high and is therefore an important public health concern. The Public Health Department of Ghana Health Service should therefore embark on health promotion and deworming exercise in all prisons in Ghana.

Prevalence and Molecular Identification of Cryptosporidium Species among Human Population in Zakho District, Duhok Province, Kurdistan Region, Iraq

This cross-sectional study was carried out to determine the prevalence and molecular identifications of Cryptosporidium species among the human population in Zakho district, Duhok Province, Kurdistan Region, Iraq. The study was performed during the period from August 2021 to March 2022 and involved 700 fecal specimens of both genders and different ages collected from Zakho General Hospital and pri¬mary schools located in the suburban area of Zakho City. The data were analyzed using statistical soft¬ware version SPSS (25). Cryptosporidium species oocyst was found in 87.57% (613/700) of the examined specimens using the Modified Ziehl Neelsen technique. Males showed a slightly higher infection rate than females (88.4% vs 85.5%) with a non-significant difference (P&gt; 0.05). The highest infection rate (91.7%) was present in individuals between the ages of &gt;30 to 40 years, with a statistically significant difference (P&lt;0.05). Molecular identification of Cryptosporidium species was estimated using 18S-rRNA gene for Cryptosporidium genus using Nested PCR technique and genus-specific primers (Cry18 AS1 and Cry18 S2) that yielded 347 bps product indicating Cryptosporidium genus, then species-specific (Cry18 AS1 and Cry18 S1) were used for the detection of Cryptosporidium parvum and Cryptosporidium hominis. 45/50 samples were positive and revealed 240 bps. The acquired sequences were deposited into the GeneBank database under the accession numbers OP107010 for C. hominis, OP104444, OP153870, OP163188, OP163414, and OP164631 for C. parvum.

Cryptosporidium infection in diarrheal bovine dairy calves: occurrence and risk factors in Santa Catarina, Brazil

Cryptosporidium protozoa genus are parasites that cause acute enteric disease in young and immunocompromised animals, resulting in anorexia, loss and decrease in weight gain, and, in severe cases, death. Therefore, this study aimed: i) to determine the occurrence of Cryptosporidium spp. in calves with clinical diarrhea in different regions of Santa Catarina, Brazil; ii) to evaluate the risk factors involved with the frequency of infection. iii) to determine the species most involved with the disease in the region. For this, 425 samples were collected in 141 dairy farms, from animals with ages ranging from 0 to 150 days. For this purpose, the samples were submitted to the modified Ziehl-Neelsen technique, with molecular analysis of the positive samples being performed. It was observed 62.1% occurrence of Cryptosporidium spp. in this sampling, especially between 8 to 15 days. Regarding the risk factors evaluated, such as age, management, facilities, water source and Koppen climate (CFA and CFB), none showed statistical significance. Samples positive by the Ziehl-Neelsen technique (32 samples) were randomly selected for molecular diagnosis. Of these, 10 were sequenced, allowing the identification of Crypstosporidium parvum in 6 samples. However, this study proves the existence and high occurrence of the protozoan in different regions of the state of Santa Catarina, Brazil.

Molecular and phylogenetic characterization of Cryptosporidium species in the saffron finch Sicalis flaveola

BackgroundCryptosporidium is the most common protozoan that can infect a wide variety of animals, including mammals and birds. Fecal samples of six saffron finches, Sicalis flaveola, from a commercial establishment were screened for the presence of Cryptosporidium by the modified Ziehl–Neelsen technique and nested PCR of the 18S rRNA gene followed by sequencing of the amplified fragments.ResultsThe species Cryptosporidium galli was identified in all six saffron fiches, in addition to Cryptosporidium andersoni in one of the birds, indicating a mixed infection. Only two birds had feathers that were ruffled and dirty with feces. Concomitant infection with Isospora spp. was observed in all birds.ConclusionsSaffron finches are a possible host of C. andersoni and this is the first report of this species in a captive bird and the third report of parasitism by C. galli in Sicalis flaveola.

Performance of diagnostic assays used to detect Cryptosporidium oocysts in faecal samples of cattle in Kuwait and genotyping of Cryptosporidium species

BackgroudCryptosporidium species are zoonotic protozoan parasites responsible for gastroenteritis in various animals and humans. The diagnosis of Cryptosporidium presents many challenges. This research attempted to match the diagnostic efficiency of the modified Ziehl–Neelsen technique (mZN), immunochromatographic assays (IC), and enzyme-linked immunosorbent assay (ELISA) for the detection of Cryptosporidium in faecal samples of cattle in Kuwait. In addition, polymerase chain reaction (PCR) was utilised to determine the predominant species infecting cattle in Kuwait and correlating the detected species with the results of different diagnostic tests used, the presence or absence of clinical signs, and the age group of the infected cattle.ResultsOf 400 analysed faecal samples, Cryptosporidium positive samples were 23%, 15.25%, and 14% using IC, ELISA, and mZN. IC had the highest sensitivity (74.07%), and mZN had the highest specificity (98.29%) using a composite reference standard (CRS) as a gold standard. The rapid IC test results in high false-positive results of cryptosporidiosis, whereas using mZN alone is insufficient to declare a negative faecal sample. Only 74.5% (35/47) of Cryptosporidium-positive samples by the three assays could be amplified by PCR. This study was the first to genotype Cryptosporidium in Kuwait. Cryptosporidium parvum (n = 26) was the dominant species detected from cattle samples, followed by C. andersoni (n = 6), C. bovis (n = 2), and C. raynae (n = 1). The findings showed a statistically relevant relationship between diarrhoea and the detection of Cryptosporidium spp. in faecal samples of cattle (p-value = 0.0003). Pre-weaned calves were the most vulnerable age group to Cryptosporidium spp. infection (p-value = 0.0007).ConclusionFor screening of Cryptosporidium infection in faecal samples, antigen detection or PCR methods combined with one of the microscopy techniques should be used. Cryptosporidium parvum was the prepoderant Cryptosporidium spp. recovered from cattle samples in Kuwait followed by C. andersoni. Cryptosporidium parvum is a significant risk factor for diarrhoea in pre-weaned calves. However, further study is needed as many other causes of diarrhoea in calves must be ruled out before a diagnosis of Cryptosporidium diarrhoea can be made.

Prevalence of Cryptosporidium infection in cattle from selected commercial farms and nomadic settlements in Yola, Adamawa State

Cryptosporidium species are apicomplexan parasites commonly associated with diarrhoea in both men and animals. They are of public health importance. The study aimed to determine the occurrence of Cryptosporidium species in cattle in the Yola metropolitan area, Adamawa State, Nigeria. Four hundred and sixteen (416) faecal samples were collected from cattle in commercial farms and nomadic settlements and were analyzed using Modified Ziehl Neelsen (MZN) technique. Twenty-seven (27) positive samples were subjected to nested Polymerase Chain Reaction (PCR) for the amplification of a specific fragment of 18S rRNA gene that was used to detect Cryptosporidium spp. Seventy-three (17.5%) out of 416 samples were positive for Cryptosporidium oocysts by MZN, and 26 (96.0%) out of 27 isolated oocysts of Cryptosporidium detected by MZN were positive by PCR. Commercial farms had a higher prevalence (19.2%) of Cryptosporidium oocysts than the nomadic settlements (15.9%). Based on risk factors, there was a significant association (P&lt;0.05) between the occurrence of Cryptosporidium oocysts and factors such as sex and faecal consistency. At the same time, age, breed, management system, animal source and drinking water source varied insignificantly (P&gt;0.05). This study has shown the occurrence of Cryptosporidium infection in cattle in the Yola metropolis. There should be an awareness campaign among public health workers on the dangers posed by the organism to humans so that the Government would enforce control and preventive measures.

Risk factors for Cryptosporidium infection in small ruminants in northern Greece

The knowledge of risk factors for Cryptosporidium spp. infection in small ruminants is based on limited data. Therefore, the current research aimed to describe the prevalence and risk factors associated with the occurrence of Cryptosporidium infection in sheep and goat herds in northern Greece. Hence, 530 fresh fecal samples from 59 sheep and goat farms were collected and examined for Cryptosporidium oocysts using microscopy of fecal smears stained by the modified Ziehl-Neelsen technique. The overall prevalence of Cryptosporidium infection for both host species was 34% (180/530; 95% confidence interval (CI): 29.9–38). Specifically, the prevalence for sheep and goats was 33.5% (112/334; 95% CI: 28.4–35.6) and 34.7% (68/196; 95% CI: 28–41.4), respectively. Additionally, standardized questionnaires were filled-in to collect data regarding animals’ health status, feeding, and other management practices in each farm. In total 22 risk factors hypothesized to be associated with Cryptosporidium infection were investigated. Multiple logistic regression analysis showed that farms with stagnant water were 11.78 (95% CI: 66–61.5) times more likely to be infected with Cryptosporidium than farms without stagnant water (p < 0.05). Furthermore, farms with more than 25% of their animals suffering from diarrhea were 17.39 (95% CI: 3.43–88.3) times more likely to be infected with Cryptosporidium than farms with ≤ 25% of the animals having diarrhea (p < 0.05). These results suggest that the animal health status and the prevailing environmental conditions play an important role in transmitting Cryptosporidium spp. infection.

Evaluating a Modified Ziehl-Neelsen Technique with Triton X-100 for Detecting Acid-fast Bacilli in Sputum

Evaluating a Modified Ziehl-Neelsen Technique with Triton X-100 for Detecting Acid-fast Bacilli in Sputum

Prevalence of Cryptosporı̇dı̇um spp. in Dogs in The Aegean Region

Cryptosporidium spp. is a protozoal parasite that can cause significant gastrointestinal diseases in humans and animals. Those parasites cause infection, especially in young and immunosuppressed animals in many mammals, poultry, reptiles, and humans, Cryptosporidium spp. Localised in the digestive system, epithelial cells threaten human and animal health with their zoonotic properties. The study's animal material consisted of 200 dogs of different breeds, ages and genders, including healthy (n=50) and diarrhoea (n=150). Stool samples of the dogs used in the study were stained with modified Ziehl-Neelsen technique and examined under a microscope. It was concluded that the prevalence of Cryptosporidium spp. was 15.5% in faecal samples taken from dogs and that the prevalence of Cryptosporidium spp. was 14% and 16%, respectively, in healthy and diarrheal dogs, and it was concluded that it could be used as a reference to future studies in dogs.

Intestinal Parasites in Children up to 14 Years Old Hospitalized with Diarrhea in Mozambique, 2014-2019.

Diarrhea remains a public health problem in Mozambique, even with control strategies being implemented. This analysis aimed to determine the proportion and factors associated with intestinal parasitic infection (IPI) in children up to 14 years old with diarrheal disease, in the southern, central and northern regions of Mozambique. A single diarrheal sample of 1424 children was collected in hospitals and examined using the formol-ether concentration and modified Ziehl–Neelsen techniques to identify intestinal parasites using optical microscopy. Sociodemographic characteristics were obtained by questionnaires. Descriptive statistics and cross-tabulation were performed, and p-values <0.05 were considered statistically significant. A single IPI was detected in 19.2% (273/1424) of the children. Cryptosporidium spp. was the most common parasite (8.1%; 115/1424). Polyparasitism was seen in 26.0% (71/273), with the co-infection of Ascaris lumbricoides and Trichuris trichiura (26.8%; 19/71) being the most common. Age and province were related to IPI (p-value < 0.05). The highest occurrence of IPI was observed in the wet period (October to March), with 21.9% (140/640), compared to the dry period (April to September), with 16.9% (131/776) (p-value = 0.017). Cryptosporidium spp. and the combination of A. lumbricoides/T. trichiura were the main intestinal parasites observed in children hospitalized with diarrhea in Mozambique.

Identification of Cryptosporidium parvum in Stool Specimen Using Different Diagnostic Methods in Wasit Province/Iraq

The present study aimed to investigate the presence and prevalence of Cryptosporidium amongimmunocompromised patients in Wasit Hospitals and health centers to determine the best method for itsdiagnosis. The study started from the 1st of August to the end of November 2020. One hundred forty stoolsamples were collected from immunocompromised patients from both genders who attended the hospitaland health centers at Wasit province. Stool samples were inspected by modified acid-fast stain as a standardmethod, enzyme linked immunosorbent assay (ELISA) and Molecular Nested PCR method. Modified Ziehl-Neelsen technique revealed 46 (33%) positive cases for Cryptosporidium oocysts, of 85 males and 55 females,highly significant relationship was found between the genders and between different age groups of infectionwith a highly significant difference between rural and urban area. The prevalence of Cryptosporidium was57.14% using nested PCR and 38.46% for ELISA in comparison with MZN method. It is concluded thatcryptosporidiosis found to be endemic in Wasit Province for the first time and the nested PCR was the mostreliable technique for its diagnosis.

Predictors of intestinal parasite infection among HIV patients on antiretroviral therapy in Jos, Plateau State, Nigeria, 2016: a cross-sectional survey.

Introductionintestinal parasitic infection has been reported as a cause of morbidity and mortality among HIV patients on antiretroviral therapy (ART) due to interruption in treatment of the defaulting HIV patients. This study aimed to determine the prevalence and possible causes of intestinal parasites among HIV patients on ART.Methodsa survey involving 375 adult HIV/AIDS patients selected using a systematic random sampling technique was conducted in a Jos University Teaching hospital, Plateau State, Nigeria. Socio-demographic and clinical data was collected using semi-structured interviewer administered questionnaire and electronic dataset review. Fresh stool samples were collected from all participants for laboratory identification of intestinal parasites using formol-ether sedimentation and modified Ziehl-Neelsen techniques. Descriptive statistics, odds ratio and logistic regression model were computed at P ≤ 0.05.Resultsthe mean age of the study participants was 41.6±9.3years. Majority 294 (78.4%) were females, 141 (37.6%) lived in the rural area, 50 (13.3%) respondents did not have toilets in their homes. Most 275 (73.3%) had ART adherence level of 95% and above. Prevalence of intestinal parasites was 28.5%. Females (aOR = 2.14, 95% CI=1.12 – 3.89) and participants with no toilet facilities (aOR = 2.0, 95% CI=1.03 – 3.94) were significantly more likely to have intestinal parasites.Conclusionthe prevalence of intestinal parasites was high among HIV patients. Gender and unavailability of toilet in homes were found to be predictors of having parasites. We recommend that HIV patients should be periodically screened for IPs during the follow-up clinic visits.

Rearing system with nurse cows and risk factors for Cryptosporidium infection in organic dairy calves

Rearing dairy calves with nurse cows has been increasingly adopted by French farmers especially in organic farming and is characterized by a fostering of two to four calves during the first month of life by an unmilked lactating cow. This type of rearing remains poorly documented regarding its impact on calf health, such as cryptosporidiosis. The objectives of our study were to describe practices related to rearing dairy calves with nurse cows and to evaluate the prevalence, intensity and risk factors for Cryptosporidium infection in calf neonates. Between January and September 2019, the rearing practices of calves were described in 20 organic French farms and faeces were sampled once from 611 animals aged between 5 and 21 days. Cryptosporidium oocyst shedding was identified by modified Ziehl-Neelsen technique and scored semi-quantitatively (score 0–4). The risk of excretion (score 0 versus 1–4) was analysed using multivariate logistic regression models.This cow-calf rearing system usually consisted of a first phase with the dam, followed by an optional phase of artificial milk feeding (calves being fed with whole milk of the farm) and a final phase of fostering by a nurse cow. Each nurse was suckled from one to five calves of close age with a fostering age of 8 days on average. The oocyst shedding prevalence was 40.2 % and similar to classically reared calves, but the intensity of shedding and the prevalence of diarrhoea appeared to be lower. The identified six risk factors for oocyst shedding were: born in the last two thirds of the birth order, born between January and July versus August and September, calf with its dam in the barn versus on pasture, having an artificial milk feeding phase versus being with the dam only, and contact between peer calves and notably the presence of an oocyst excretory calf fostered by the same nurse. These results emphasize the role of the environment for the direct and indirect contamination, particularly that related to the accumulation of oocysts from previous or peer calves facilitating the faecal-oral route of transmission. This highlights the crucial role of the premises used intensively during the winter and spring months with higher densities of calves in the barn compared to outdoor situations promoted by this rearing.

Prevalence of &amp;lt;i&amp;gt;Cryptosporidium&amp;lt;/i&amp;gt; Species and Other Intestinal Parasites Among HIV Infected Patients at LAUTECH Teaching Hospital, Osogbo, Nigeria

<i>Background</i>: Cryptosporidiosis though an opportunistic infection in HIV infected patients, increases the mortality and morbidity to the diseases by its effect suppression of the immune system, our quest is to highlights the clinical correlation of diarrhea in immunocompromised individuals and the economic losses associated with managing infected individuals. <i>Methods</i>: Stool samples were collected from 96 subjects. Their personal information regarding their names, sex, age was also obtained. The stool samples were processed for non-opportunistic parasite using the direct and formol-ether concentration method. For Cryptosporidium, Modified Ziehl-Neelsen Technique was used. A total of ninety-six confirmed HIV individuals were enrolled for this study. <i>Results</i>: The mean age of the patients was 24.7 years with the youngest been 3 years old and the eldest 58 years old. 77 (74%) were females while 22 (23%) were males. Table 1 shows the general characteristics of the HIV subjects recruited into the study. The distribution of intestinal parasites detected in patient’s stool is shown in Table 2. Of the 96 stool samples examined, intestinal parasites were seen in 87 samples, while 9 samples showed absence of parasites. The diagnosed parasitic infections were: <i>Cryptosporidium</i> spp (54.2%), <i>A. lumbricoides</i> (9.4%), Hookworm (5.2%), <i>E. histolytica</i> (3.1%), <i>S. stercoralis</i> (1%) and Teania spp (1%). Double parasitic infections with Cryptosporidium were observed in 13 (13.5%) HIV/AIDS patients stool samples. Regarding age and sex distribution of the participants, there were 74 (77%) females and 22 (23%) males. Males (68.2%) were more infected than females (55.4%) but the difference was not statistically significant. Table 3 shows relationship between intestinal parasitic infection and diarrhea in the HIV/AIDS patients. 45 (86.5%) of patients with Cryptosporidium infection presented with diarrhea. <i>Conclusion</i>: The association between diarrhea and Cryptosporidium infection was statistically significant. It may be concluded that in HIV infected patients, both opportunistic intestinal parasitic infections are prevalent.

Distribution of Cryptosporidium and Cyclospora at Different Depths in Underground Water in the Centre Region, Cameroun: Role of Hydrodynamical Variable

This study, developed in Centre Region of Cameroon, made it possible to indicate the consequences responsible of soil vulnerability. Chemical and biological analysis of soil samples collected near wells and springs during the short rainy season were carried out. The results show that the pH and electrical conductivity values decrease with the depth respectively 6.11 CU and 127.47 µS/cm at the surface (0cm) follow by 5.52 CU and 69.32 µS/cm at 50 cm depth. The hydraulic conductivity shows that the soil is moderately permeable (10-5m/s). Protozoa oocysts were observed using an Olympus CK2 inverted microscopy at 40X objective using Zinc sulphate flotation and modified Ziehl-Neelsen technique. The oocysts were counted using a proposal formula. The distribution of oocysts decreases with depth. At the surface of the soil (0cm) the densities of Cryptosporidium and Cyclospora are respectively 123 oocysts/100g and 50 oocysts/100g. At 25cm depth, the densities of Cryptosporidium and Cyclospora are respectively 36 oocysts /100g and 6 oocysts /100g. At 50cm depth the densities of Cryptosporidium and Cyclospora are respectively 16 oocysts/100g and 3 oocysts/100g. The contamination of these different depth layers would be a risk of contamination of groundwater.

Assessments of the level of adherence to antiretroviral therapy and the health status of people living with HIV in Calabar, Nigeria

Background: Medication adherence describes a patient's behavior of accurately following drug regimens; nonadherence describes a patient's inability to accurately follow drug regimens. Several factors such as psychosocial, educational, health-related, and environmental-related factors could contribute in determining the level of adherence to antiretroviral drug. This was a study to assess the level of adherence antiretroviral combination therapy and the health status of people living with HIV in Calabar, Nigeria. Materials and Methods: Questionnaires were administered to 350 HIV persons who attended antiretroviral treatment (ART) clinic to obtain their sociodemographic data, knowledge of ART, service received, and drug history. Stool samples were collected and examined using direct microscopy, formol–ether concentration technique, and modified Ziehl–Neelsen technique for enteric parasites, whereas blood samples were collected for HIV status examination using serial HIV testing algorithm, hemoglobin levels using cyanmethemoglobin method, and counting of CD4 using Partec CyFlow counter. Results: The results showed that 79.43% (278/350) of the participants adhered to antiretroviral drugs, with most of them (47.84%, 133/278) having CD4 counts between 201 and 400 cell/μl. Stigma was the major reason for nonadherence to treatment. Those who adhered to therapy had a lower infection rate and an elevated mean hemoglobin level than those who did not adhere. Conclusion: There was also an elevated hemoglobin level, lower enteric parasite infections, and an improved CD4 count among the adherents than those who did not adhere.

Cryptosporidium and giardia species in newly and previously habituated gorillas and nearby water sources in Bwindi Impenetrable National Park, Uganda.

Introductioncryptosporidium and giardia are of great one health significance and major cause of protozoan diarrhea in humans and primates; they are found in the faecal matter of animals and humans and also in contaminated water and soil as well. Therefore, we aimed at establishing the prevalence and shedding intensity of faecal Cryptosporidium and giardia in the Newly Habituated Mountain Gorillas (NHMG) and Previously Habituated Mountain Gorillas (PHMG) and in selected water sources within the gorilla home ranges in the month of January 2018.Methodswe conducted a cross sectional study in the southern sector of Bwindi Impenetrable National Park where a total of 56 faecal samples from both NHMG (34) and PHMG (22) and 30 water samples were purposively collected. Faecal and water samples were transported in a cooler box at 4ºC to Makerere University Parasitology Laboratory for analysis. The samples were analyzed using modified Ziehl-Neelsen technique and Ether concentration method for Cryptosporidium and giardia respectively.Resultsthe prevalence of cryptosporidium was established as 13 (59.1%), 15 (44.1%) and 7 (23.3%) in PHMG, NHMG and water respectively. The mean concentration of the oocysts per gram was 222±52.9 in PHMG, 174±41.5 in NHMG and 31±13.2 in water. The prevalence of giardia was 3 (13.6%), 4 (11.8%) and 3 (10%) in PHMG, NHMG and water respectively. The mean concentration of the oocysts per gram was 34±19.9 in PHMG, 25±12.4 in NHMG and 5±2.9 in water. There was no significant difference in both the prevalence of cryptosporidium (p>0.05) and giardia (p>0.05) in the PHMG and NHMG. This indicates that there is high risk of cross infection among the gorillas within the forest sharing similar home ranges.Conclusionthe park authorities should ensure that procedures for proper waste disposal while in the forest are properly followed, water drawn for drinking from the forest should be avoided. Further research should be carried out to identify whether the strains of the parasites found in water or other animals in the forest are the same with strains in gorilla dung in order to confirm cross infection.

Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina.

Cryptosporidiosis of calves is caused by the enteroprotozoan Cryptosporidium spp. The disease results in intense diarrhea of calves associated with substantial economic losses in dairy farming worldwide. The aim of this study was to determine calf, herd, and within-herd Cryptosporidium prevalence and identify Cryptosporidium species and subtypes in calves with diarrhea in intensive dairy herds in central Argentina. A total of 1073 fecal samples were collected from 54 randomly selected dairy herds. Cryptosporidium-oocysts were isolated and concentrated from fecal samples using formol-ether and detected by light microscopy with the modified Ziehl-Neelsen technique. Overall prevalence of oocyst-excreting calves was found to be 25.5% (274/1073) (95% C.I. 22.9; 28.1%). Of the herds studied, 89% (48/54) included at least one infected calf, whereas within-herd prevalence ranged from the absence of infection to 57% (20/35). A highly significant association was found between the presence of diarrhea and C. parvum infection (χ2 = 55.89, p < 0.001). For species determination, genomic DNA isolated from oocyst-positive fecal samples was subjected to PCR-RFLP of the 18S rRNA gene resulting exclusively in Cryptosporidium parvum identification. C. parvum isolates of calves displaying diarrhea and high rate of excretion of oocysts were subtyped by PCR amplification and direct sequencing of the 60 kDa glycoprotein (GP60) gene. Altogether five GP60 subtypes, designated IIaA18G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1, and IIaA24G1R1 were identified. Interestingly, IIaA18G1R1 and IIaA20G1R1 were predominant in calves with diarrhea and high infection intensity. Notably, IIaA24G1R1 represents a novel, previously unrecognized C. parvum subtype. The subtype IIaA18G1R1, frequently found in this study, is strongly implicated in zoonotic transmission. These results suggest that calves might be an important source for human cryptosporidiosis in Argentina.Electronic supplementary materialThe online version of this article (10.1007/s00436-019-06366-y) contains supplementary material, which is available to authorized users.

&lt;p&gt;Prevalence of cryptosporidiosis and hygiene practices among HIV/AIDS patients in southwest Uganda&lt;/p&gt;

Purpose: To determine the prevalence of Cryptosporidium by age, sex, and duration on antiretroviral therapy (ART) and establish hygienic malpractices that may predispose to infection.Methods: We enrolled 138 HIV/AIDS patients on ART from June to October 2018. Stool samples were collected from study participants, wet saline preparations made and examined, stool samples concentrated using formal ether concentration, and smears stained using the modified Ziehl–Neelsen technique. Structured questionnaires were used to collect demographic data and hygienic malpractices that predisposed study participants to cryptosporidiosis infection.Results: Of 138, 99 (71.7%) were females and 39 (28.7%) males. The age range was 9–69 years and mean age 37 years. The overall prevalence of cryptosporidiosis was three (2.17%). The most affected age-groups were 31–40 years (3.85%) and 21–30 years (3.22%), and only females (3.03%) were affected. The distribution of cryptosporidiosis according to the duration spent on ART showed that those who had spent <1 year on ART were the most affected (11.1%), followed by those who had spent 1–5 years 1 (2.2%), while those patients on ART for 6-10 years were 1 (1.7%) and those on ART for more than 10 years were not affected. There was no significant association between cryptosporidiosis and sex (P=0.272), educational background (P=0.670), handwashing (P=0.853), drinking boiled water (P=0.818), duration on ART (P=0.263), occupation (P=0.836), and age (P=0.723).Conclusion: The prevalence reported in this study is low; however, it is still vital for clinicians to proceed to have cryptosporidiosis as the main differential in HIV/AIDS patients with gastrointestinal infections.