Modification Of Gelatin Research Articles

Investigation of the Effects of Phenolic Extracts Obtained from Agro-Industrial Food Wastes on Gelatin Modification.

In this study, modified bovine gelatin was produced using the alkaline technique with four different oxidized agro-industrial food waste (pomegranate peel (PP), grape pomace and seed (GP), black tea (BT), and green tea (GT)) phenolic extracts (AFWEs) at three different concentrations (1, 3, and 5% based on dry gelatin). The effect of waste type and concentration on the textural, rheological, emulsifying, foaming, swelling, and color properties of gelatin, as well as its total phenolic content and antioxidant activity, was investigated. Significant improvement in gel strength, thermal stability, and gelation rate of gelatin was achieved by modification with oxidized agro-industrial waste extracts. Compared to the control sample, 46.24% higher bloom strength in the GT5 sample, 5.29 and 6.01 °C higher gelling and melting temperatures in the PP5 sample, respectively, and 85.70% lower tmodel value in the GT3 sample were observed. Additionally, the total phenolic content, antioxidant activity, foam, and emulsion properties of the modified gels increased significantly. This study revealed that gelatins with improved technological and functional properties can be produced by using oxidized phenolic extracts obtained from agricultural industrial food wastes as cross-linking agents in the modification of gelatin.

Modification of Gelatin by Ciprofloxacin for Industrial Applications

Generally, gelatin resulting from collagen, a naturally occurring protein found in ligaments and tissues, is formed through the boiling of connective tissues, bones and animal skins, commonly sourced from cows. It has exceptional properties, including the ability to form strong, transparent gels and flexible films, easy digestibility, solubility in hot water and positive binding action, make gelatin a highly valuable resource in various industries such as food processing, pharmaceuticals, photography and paper production. It is regarded as non-toxic, non-degradable and biocompatible material. In this study we modified gelatin by ciprofloxacin compound, specifically we aimed gelatin to be used in different industries specifically in food packaging, then the synthesized materials were characterized by Fourier transform infrared (FTIR), the surface was imaged by scanning electron microscopy (SEM) furthermore, surface morphology was measured using atomic force microscopy (AFM). Overall, all analysis techniques confirmed the modification by the presence of ciprofloxacin coordinated with the gelatin molecule.

Injectable microcapillary network hydrogels engineered by liquid-liquid phase separation for stem cell transplantation

Injectable hydrogels are promising carriers for cell delivery in regenerative medicine. However, injectable hydrogels composed of crosslinked polymer networks are often non-microporous and prevent biological communication with host tissues through signals, nutrients, oxygen, and cells, thereby limiting graft survival and tissue integration. Here we report injectable hydrogels with liquid-liquid phase separation-induced microcapillary networks (μCN) as stem cell-delivering scaffolds. The molecular modification of gelatin with hydrogen bonding moieties induced liquid-liquid phase separation when mixed with unmodified gelatin to form μCN structures in the hydrogels. Through spatiotemporally controlled covalent crosslinking and dissolution processes, porous μCN structures were formed in the hydrogels, which can enhance mass transport and cellular activity. The encapsulation of cells with injectable μCN hydrogels improved cellular spreading, migration, and proliferation. Transplantation of mesenchymal stem cells with injectable μCN hydrogels enhanced graft survival and recovered hindlimb ischemia by enhancing material-tissue communication with biological signals and cells through μCN. This facile approach may serve as an advanced scaffold for improving stem cell transplantation therapies in regenerative medicine.

The Effect of Gelatin Source on the Synthesis of Gelatin-Methacryloyl and the Production of Hydrogel Microparticles.

Gelatin methacryloyl (GelMA) is widely used for the formulation of hydrogels in diverse biotechnological applications. After the derivatization of raw gelatin, the degree of functionalization (DoF) is an attribute of particular interest as the functional residues are necessary for crosslinking. Despite progress in the optimization of the process found in the literature, a comparison of the effect of raw gelatin on the functionalization is challenging as various approaches are employed. In this work, the modification of gelatin was performed at room temperature (RT), and eight different gelatin products were employed. The DoF proved to be affected by the bloom strength and by the species of gelatin at an equal reactant ratio. Furthermore, batch-to-batch variability of the same gelatin source had an effect on the produced GelMA. Moreover, the elasticity of GelMA hydrogels depended on the DoF of the protein as well as on bloom strength and source of the raw material. Additionally, GelMA solutions were used for the microfluidic production of droplets and subsequent crosslinking to hydrogel. This process was developed as a single pipeline at RT using protein concentrations up to 20% (w/v). Droplet size was controlled by the ratio of the continuous to dispersed phase. The swelling behavior of hydrogel particles depended on the GelMA concentration.

Facile strategy for gelatin-based hydrogel with multifunctionalities to remodel wound microenvironment and accelerate healing of acute and diabetic wounds

Chronic diabetic wounds represent the most common diabetes complication. Wound healing depends on scavenging reactive oxygen species (ROS), neovascularization, and controlling infection. A naturally derived gelatin-based hydrogel is biocompatible, biodegradable, does not promote inflammation, and can remove ROS, but strategies for developing a gelatin-based hydrogel currently require careful chemical modification of gelatin and time-consuming purification and post-crosslinking processing. Herein, a facile method of combining zirconium (Zr4+), gelatin, and quercetin (QCN) to generate an injectable gelatin-based hydrogel (QCN@Gel-Zr) for diabetic wound treatment was presented. Adding QCN improved the mechanical, injection, and adhesive performance of the Gel-Zr hydrogel and conferred antibacterial and free radical–scavenging abilities. These properties induced cellular proliferation and migration, protection against oxidative stress, and reduction in inflammatory expression. In vivo models of acute and chronic diabetic skin wounds were used to demonstrate biocompatibility and the ability of the gelatin hydrogels to promote wound healing. The histological analysis showed that the QCN@Gel–Zr hydrogel promoted angiogenesis, collagen deposition, and hair follicle regeneration with no detectable cytotoxicity. This study demonstrates the preparation of gelatin-based hydrogel with various flexible functions to address the complex biological requirements of diabetic wound repair.

Construction of gigantic highly-porous microcarriers as a three-dimensional cell culture platform

3D cell carriers with porous structures have the potential to promote cellular interactions and extracellular matrix remolding, making it a promising strategy to investigate the complex mechanisms behind drug treatments for tumors. Nevertheless, the applicability of currently reported porous microcarriers is still limited by the relatively small size and poor pore interconnectivity. Herein, a well-defined gigantic highly-porous poly(lactic-co-glycolic acid) (PLGA) microsphere (named LPMs) fabrication platform is reported. Utilizing this “LPMs” platform, arrays of well-uniform particles with sizes up to approximately 1000 µm are prepared in an orderly and controlled manner. The preparation matrix and crucial parameters affecting the particle size and pore size were established through extensive single-factor screening. It was also found that the loading of human gastric cancer cells MGC-803 to the LPMs cell carriers was facilitated not only by the increased pore size, but the particle size should also be enlarged along with the increased pore size. Through surface modification of gelatin on LPMs, cell loading could be dramatically enhanced, and microtissue-like structures were formed through cross-linking LPMs under extended 3D culture time. Under the guidance of these findings, we successfully constructed massive tumor cell carriers with 1000 µm LPMs, a preliminary prototype of a massive tumor tissue mimic. Together, the fabrication of these gigantic highly-porous microcarriers possibly allows for the high-efficiency 3D cell culture and represents the attractive potential of the construction of artificial tumor tissue models with massive and multiple-type cells.

Microfluidic Fabrication of Gelatin Acrylamide Microgels through Visible Light Photopolymerization for Cell Encapsulation.

Gelatin-based microgels are intriguing for various biomedical applications, which are conventionally prepared through photopolymerization of gelatin methacrylamide (GelMA). Here, we report on the modification of gelatin through acrylamidation to form gelatin acrylamide (GelA) with different substitution degrees, which was found to exhibit fast photopolymerization kinetics, better gelation, steady viscosity at elevated temperatures, and satisfying biocompatibility when compared to GelMA. By the online photopolymerization strategy with a home-made microfluidic setting, microgels of uniform sizes from GelA by blue light were obtained and their swollen properties were investigated. Compared to the microgels from GelMA, they showed an enhanced cross-linking degree and have better shape stability when swollen in water. Cell toxicities of the hydrogels from GelA and cell encapsulation from the corresponding microgels were investigated, which were found to exhibit superior properties than those from GelMA. We therefore believe that GelA has potential for constructing scaffolds for bioapplications and can be an excellent substitute for GelMA.

Modification of gelatin from tuna skins by green tea polyphenols

This paper presents the result of study on the effect of modifying conditions on properties and structure of tuna skin gelatin by using green tea polyphenols. The results showed that gelatin modified by green tea polyphenols changed its gel strength, degree of cross-linking, molecular weight and gel structure. The appropriate parameters to modify gelatin by using green tea polyphenols which indicated the best mechanical properties were determined as follows: temperature of 40oC in 40 minutes, polyphenol content of 20 mg/g gelatin and gelatin solution of 20%. As a result, modified gelatin showed a decrease in solubility, an increase in cross-linking degree (16.7%), molecular weight (55÷72 kDa) as well as a larger protein structure in comparison with the natural gelatin. The IR spectrograph of the modified gelatin did not show any evidence of forming new functional groups, only an apparent change in the absorption of infrared spectroscopy at the peak of the amide B region was found. The dried modified gelatin became insoluble in hot water.

Extraction Optimization and Physical Modification of Fish Gelatin from Thunnus tonggol Using Ultraviolet (UV) Irradiation

ABSTRACT In this study, the yield of gelatin extraction from fish waste was optimized using response surface method. The highest yield was obtained with 0.8% citric acid concentration at 72.95°C for 58 minutes. Then, fish gelatin was modified using ultraviolet radiation to overcome fish gelatin defects including unsuitable rheological properties and low thermo-stability. UV irradiation can create crosslinks via alkyl or phenyl groups of the proteins. The modified sample also showed the highest melting point due to the creation of cross-links and improved emulsification and foaming properties of fish gelatin through structural modification and increased surface hydrophobicity of gelatin.

Injectable hydrogels of enzyme-catalyzed cross-linked tyramine-modified gelatin for drug delivery

Enzymatically catalyzed cross-linking is a hydrogel fabrication method that generally is considered to have lower cytotoxicity than traditional chemical cross-linking methods. In order to optimize the properties of injectable hydrogels and expand their applications, an enzyme-catalyzed cross-linked injectable hydrogel was designed. The tyramine-modified gelatin (G-T) was formed into a stable injectable hydrogel by the combination of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) catalysis. 1H NMR spectroscopy was used to demonstrate the successful modification of gelatin by tyramine. The surface morphology of the prepared hydrogels was characterized jointly by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Rheological tests demonstrated the tunable mechanical strength, formation kinetics, shear thinning and good self-recovery properties of the hydrogels. In addition, the hydrogels can be formed into various shapes by injection. The hydrogel network structure is complex and interlaced, as such it is suitable to encapsulate drugs for controlled release. The drug release from the prepared hydrogels followed the Peppas–Sahlin model and belonged to Fickian diffusion. This study constructed injectable hydrogels through the enzyme-catalyzed cross-linking of modified gelatin and applied the hydrogels for drug release, which is expected to expand the application in biomedical fields.

Modification of gelatin and photocured 3D‐printed resin to prepare biomimetic phantoms for ultrasound‐guided minimally invasive surgeries

AbstractThe main challenge of ultrasound‐guided minimally invasive surgeries is to carry out treatments through small boreholes in the patient's body surface with sub‐millimetric accuracy and facility offered by ultrasound instruments. Thus, the phantoms' biomimetic appearances, acoustic characteristics, and mechanical properties are essential for healthcare professionals. In this study, gelatin and 3D‐printed photo‐resin phantoms were developed for hard and soft tissues, respectively. Using glutaraldehyde (GA) and formaldehyde (FA) to crosslink gelatin phantoms, the mechanical strength, ultrasound velocity, acoustic impedance, needle insertion forces, and antiseptic periods were improved, and the addition of silica nanoparticles raised the acoustic impedance. For 3D‐printed phantoms, the acoustic impedance ascended with hydrophilic polyethylene glycol diacrylate (PEGDA) oligomers. The additives applied in this study successfully made the gelatin and photo‐resin phantoms more mimetic to muscle and bone tissues, allowing their application in preoperative preparations.

A Novel Approach for the Manufacturing of Gelatin-Methacryloyl.

Gelatin and its derivatives contain cell adhesion moieties as well as sites that enable proteolytic degradation, thus allowing cellular proliferation and migration. The processing of gelatin to its derivatives and/or gelatin-containing products is challenged by its gelation below 30 ∘C. In this study, a novel strategy was developed for the dissolution and subsequent modification of gelatin to its derivative gelatin-methacryloyl (GelMA). This approach was based on the presence of urea in the buffer media, which enabled the processing at room temperature, i.e., lower than the sol-gel transition point of the gelatin solutions. The degree of functionalization was controlled by the ratio of reactant volume to the gelatin concentration. Hydrogels with tailored mechanical properties were produced by variations of the GelMA concentration and its degree of functionalization. Moreover, the biocompatibility of hydrogels was assessed and compared to hydrogels formulated with GelMA produced by the conventional method. NIH 3T3 fibroblasts were seeded onto hydrogels and the viability showed no difference from the control after a three-day incubation period.

Polyphosphate enhanced biomimetic mineralization of 3D printing scaffolds for bone regeneration

Biomimetic mineralization has been widely applied in the preparation of bone repair materials for achieving better mechanical and biological properties. However, the mineralization effectiveness is not quite satisfactory. Herein, enhanced biomimetic mineralization was realized through polyphosphate modification of gelatin. Molecular dynamics simulations theoretically demonstrated the effectiveness of poly-phosphorylation in augmenting mineralization. Experimental results revealed that poly-phosphorylation could increase the saturation of in-situ mineralization of gelatin from about 25% to more than 40%. Consequently, the compressive strength and elastic modulus of the enhanced mineralized composite were increased by 57.93% and 132.55%, respectively. The osteogenesis was also promoted. Combined with 3D printing, the scaffolds not only had the advantages of enhanced in-situ mineralization but also were endowed with the macro-controllable porous structure to achieve better bone regeneration. It interpreted the mechanism of biomimetic mineralization in terms of the saturation of in-situ mineralization and provided a viable strategy for biomimetic scaffold preparation.

Gelatin–Siloxane Hybrid Monoliths as Novel Heavy Metal Adsorbents

Novel gelatin-siloxane hybrid monoliths for heavy metal removal were prepared in the chemical reaction of gelatin with organomodified silicone containing epoxy group. Obtained porous hybrid materials were applied for adsorption of Cu(II), Cd(II) and Pb(II) from aqueous solutions. In this paper, the influence of siloxane amount used for the modification of gelatin on adsorbent stability and heavy metal removal was examined. The effect of pH values of the immersion liquid, as well as the contact time, was studied. Morphology, compressive strength and water absorption of hybrid monoliths were investigated. Desorption tests were also performed. The results showed that the higher the amount of the siloxane, the better stability of the hybrid monoliths in aqueous solutions. The highest values of adsorption capacity were observed for Pb(II) ions. The experimental maximum adsorption capacity determined for hybrid monoliths was 3.75 mg/g for Pb(II), 1.76 mg/g for Cu(II) and 1.5 mg/g for Cd(II). The desorption of metal ions for hybrid monoliths stable in aqueous solutions reached 70%.

Biodegradable Composites with Functional Properties Containing Biopolymers

There is a major focus on natural biopolymers of bacterial, animal, or plant origin as ecological materials, replacing petrochemical products. Biologically derived polylactide (PLA), polyhydroxybutyrate (PHB), and polyhydroxyalkanoates (PHA) possess interesting properties, but they are currently too expensive for most applications. Therefore, researchers try to find other biopolymers that are both durable and cheap enough to replace plastics in some applications. One possible candidate is gelatin, which can be transformed into a thin, translucent film that is flexible and has stable and high mechanical properties. Here, we present a method of synthesizing a composite material from gelatin. For preparation of such material, we used gelatin of animal origin (pig skin) with the addition of casein, food gelatin, glycerin, and enzymes as biocatalysts of chemical modification and further extraction of gelatin from collagen. Compositions forming films with homogeneous shapes and good mechanical properties were selected (Tensile strength reaches 3.11 MPa, while the highest value of elongation at break is 97.96%). After administering the samples to microbial scaring, the composites completely decomposed under the action of microorganisms within 30 days, which proves their biodegradation.

Gelatin-Tannin-Based Greener Binder Technology for Stone Shot and Stone Wool Materials: A Detailed Study.

The detailed study presented herein of gelatins modified with tannins as greener binder systems for stone wool and related materials has unveiled a versatile technology that offers a wide range of possibilities for tailor-making properties toward various application areas. Thus, high unaged and aged mechanical strengths in combination with low water solubilities may generally be obtained from the use of gelatins with higher gel strength (and hence, generally, higher molecular weights), low-to-mid range tannin addition levels (3–20%), alkali metal hydroxides for pH adjustment, and final pH in the range 8–9. Comparatively low water uptake properties may be obtained using higher gel strength type A gelatins, lower tannin addition levels, alkali metal hydroxides for pH adjustment, and lower final pH. Even lower water uptake properties may then be obtained using Ca(OH)2 in place of alkali metal hydroxides. If desired, higher water uptakes may be obtained using type B gelatins (or lower gel strength gelatins in general), higher tannin addition levels, and higher final pH. Mechanistic studies indicated that the optimal modification of gelatin with the tannin component occurs via several pathways.

Fabrication of gelatin-based printable inks with improved stiffness as well as antibacterial and UV-shielding properties

Gelatin-based inks have a broad range of applications in bioprinting for tissue engineering and regenerative medicine due to their biocompatibility, ease of modification, degradability, and rapid gelation induced by low temperature. However, gelatin-derived inks prepared through low-temperature treatment have poor mechanical properties that limit their applications. To solve this problem, we designed polyacrylamide/gelatin/silver nanoparticle (PAAm-GelatinAgNPs) ink to improve gelatin-based hydrogels. The ink is based on double networks, in which the physically cross-linked gelatin as the first network and covalently cross-linked PAAm as the second network. It was found that the presence of PAAm increased the tensile and compression strength of the gelatin-based ink. Moreover, silver nanoparticles endowed the antibacterial properties to the gelatin-based ink and were able to shield the UV irradiation and damages to rat skin. In addition, this ink showed the shear thinning property; Consequently it succeeded in printing complex 3D scaffolds such as the cube, five-pointed star, flower, and university logo of “SEU”. In summary, this ink presents a new strategy for the modification of gelatin and offers new potential applications for customized therapy of antimicrobial and anti-UV damage to tissues.

Functionalization of Crosslinked Sodium Alginate/Gelatin Wet-Spun Porous Fibers with Nisin Z for the Inhibition of Staphylococcus aureus-Induced Infections.

Nisin Z, an amphipathic peptide, with a significant antibacterial activity against Gram-positive bacteria and low toxicity in humans, has been studied for food preservation applications. Thus far, very little research has been done to explore its potential in biomedicine. Here, we report the modification of sodium alginate (SA) and gelatin (GN) blended microfibers, produced via the wet-spinning technique, with Nisin Z, with the purpose of eradicating Staphylococcus aureus-induced infections. Wet-spun SAGN microfibers were successfully produced at a 70/30% v/v of SA (2 wt%)/GN (1 wt%) polymer ratio by extrusion within a calcium chloride (CaCl2) coagulation bath. Modifications to the biodegradable fibers’ chemical stability and structure were then introduced via crosslinking with CaCl2 and glutaraldehyde (SAGNCL). Regardless of the chemical modification employed, all microfibers were labelled as homogeneous both in size (≈246.79 µm) and shape (cylindrical and defect-free). SA-free microfibers, with an increased surface area for peptide immobilization, originated from the action of phosphate buffer saline solution on SAGN fibers, were also produced (GNCL). Their durability in physiological conditions (simulated body fluid) was, however, compromised very early in the experiment (day 1 and 3, with and without Nisin Z, respectively). Only the crosslinked SAGNCL fibers remained intact for the 28 day-testing period. Their thermal resilience in comparison with the unmodified and SA-free fibers was also demonstrated. Nisin Z was functionalized onto the unmodified and chemically altered fibers at an average concentration of 178 µg/mL. Nisin Z did not impact on the fiber’s morphology nor on their chemical/thermal stability. However, the peptide improved the SA fibers (control) structural integrity, guaranteeing its stability for longer, in physiological conditions. Its main effect was detected on the time-kill kinetics of the bacteria S. aureus. SAGNCL and GNCL loaded with Nisin Z were capable of progressively eliminating the bacteria, reaching an inhibition superior to 99% after 24 h of culture. The peptide-modified SA and SAGN were not as effective, losing their antimicrobial action after 6 h of incubation. Bacteria elimination was consistent with the release kinetics of Nisin Z from the fibers. In general, data revealed the increased potential and durable effect of Nisin Z (significantly superior to its free, unloaded form) against S. aureus-induced infections, while loaded onto prospective biomedical wet-spun scaffolds.

Underwater-adhesive microparticle dressing composed of hydrophobically-modified Alaska pollock gelatin for gastrointestinal tract wound healing

Despite the success of minimally-invasive endoscopic submucosal dissection (ESD) for the treatment of early gastrointestinal cancer, additional symptoms after ESD, including contracture, perforation, bleeding, and esophageal stricture remain. Conventional wound dressings were ineffective in preventing stricture because of poor stability of underwater-adhesives on living tissues. Here, we present a microparticle-based wound dressing with underwater adhesive stability for the treatment of gastrointestinal tract wound healing after ESD. Monodisperse microparticles composed of hydrophobically-modified Alaska pollock gelatin were prepared by self-assembly of gelatin in water-ethanol mixed solvents and thermal crosslinking. Hydrophobic modification of gelatin with aliphatic aldehydes increased adhesion strength to gastric and esophageal submucosal tissues through hydrophobic interaction with living tissues and cohesion force. Optimal hydrophobic modification drastically improved underwater stability of microparticles compared to that of non-modified gelatin and formed a thick, integrated hydrogel layer on tissues. Histological observation of rat skin wound healing models showed that hydrophobically-modified gelatin microparticles decreased the expression levels of α-smooth muscle actin in the dermis layer and could suppress fibrosis and inflammation after ESD. The microparticle wound dressing with high underwater-adhesive stability has enormous therapeutic potential to promote wound healing in the gastrointestinal tract and prevent additional symptoms after ESD. Statement of SignificanceThe goal of this study was to develop wound dressing with strong tissue-adhesive property to living tissues for promoting wound healing after ESD treatment. Monodisperse microparticles composed of hydrophobically-modified Alaska pollock gelatin were prepared by self-assembly of gelatin in water-ethanol mixed solvents and thermal crosslinking. Hydrophobic modification of gelatin with aliphatic aldehydes enhanced adhesion strength to gastric and esophageal submucosal tissues through hydrophobic interaction with living tissues and cohesion force. Optimal hydrophobic modification drastically improved underwater stability of microparticles. The in vivo studies were performed to evaluate the ability of this colloidal wound dressing to suppress fibrosis. This new biomaterial has enormous potential to promote wound healing after ESD.

Carbon nanotube, poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) and Ag nanoparticle doped gelatin based electro-active hydrogel systems

In this study, gelatin-based conductive hydrogels doped with carbon nanotube (CNT), poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and silver (Ag) nanoparticle were prepared for use in biopotential measurements. Gelatin was modified by various concentrations of methacrylic anhydride (MA) to obtain photo-crosslinkable gelatin methacrylate (GelMA) polymer with low (21%), medium (44%) and high (78%) methacrylation degree. Chemical modification of gelatin was characterized by 1H-NMR spectroscopy. It was observed that mechanical properties of the GelMA hydrogels were highly dependent on methacrylation degree. Ultimate compressive stress of low, medium and high methacrylated gelatin hydrogel was measured to be 9.97 ± 0.14 kPa, 17.83 ± 1.73 kPa and 38.23 ± 2.96 kPa, respectively. Surface morphology of hydrogels was visualized by scanning electron microscope (SEM). The pore size of methacrylated gelatin hydrogel significantly reduced with the addition of CNT, PEDOT:PSS and Ag nanoparticle into the hydrogel matrix. The results of thermal gravimetric analysis showed that conductive hydrogels were of higher thermal stability than nonconductive GelMA hydrogel. The suitability of the prepared hydrogels for biopotential measurements was determined by equivalent impedance measurements. Impedance values of hydrogel samples were obtained in the frequency range of 20 Hz–500 Hz. The hydrogel materials prepared in this study exhibited high impedance at low frequencies and low impedance at high frequencies. The results of this study showed that the hydrogels produced with Ag nanoparticle are more suitable electrode materials for physiological measurement systems such as electrocardiography (ECG) and electroencephalography (EEG).