Modification Of Activity Research Articles

Evolutionary Adaptations in Biliverdin Reductase B: Insights into Coenzyme Dynamics and Catalytic Efficiency

Biliverdin reductase B (BLVRB) is a redox regulator that catalyzes nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reductions of multiple substrates, including flavins and biliverdin-β. BLVRB has emerging roles in redox regulation and post-translational modifications, highlighting its importance in various physiological contexts. In this study, we explore the structural and functional differences between human BLVRB and its hyrax homologue, focusing on evolutionary adaptations at the active site and allosteric regions. Using NMR spectroscopy, we compared coenzyme binding, catalytic turnover, and dynamic behavior between the two homologues. Despite lacking the arginine “clamp” present in human BLVRB, hyrax BLVRB still undergoes conformational changes in response to the oxidative state of the coenzyme. Mutations at the allosteric site (position 164) show that threonine at this position enhances coenzyme discrimination and allosteric coupling in human BLVRB, while hyrax BLVRB does not display the same allosteric effects. Relaxation experiments revealed distinct dynamic behaviors in hyrax BLVRB, with increased flexibility in its holo form due to the absence of the clamp. Our findings suggest that the evolutionary loss of the active site clamp and modifications at position 164 in hyrax BLVRB alter the enzyme’s conformational dynamics and coenzyme interactions. Identified similarities and differences underscore how key regions modulate catalytic efficiency and suggest that coenzyme isomerization may represent the rate-limiting step in both homologues.

The mechanism of sevoflurane affecting ovarian cancer cell proliferation and migration by regulating RNA methylase TRDMT1 to activate the β-catenin pathway

ObjectiveSevoflurane (Sevo), a commonly used inhalant anesthetic clinically, is associated with a worsened cancer prognosis, and we investigated its effect on RNA methylase tRNA aspartic acid methyltransferase 1 (TRDMT1) expression and ovarian cancer (OC) cell malignant phenotypes.MethodsHuman OC cells (OVCAR3/SKOV3) were pretreated with 3.6% Sevo and cultured under normal conditions for 48 h, with their viability assessed. After 2-h Sevo treatment or interference plasmid transfections to down-regulate TRDMT1/adenomatous polyposis coli (APC), changes in TRDMT1, APC and β-catenin expression, cell proliferative activity, cycle, apoptosis, migration, invasion, and 5-methylcytosine (m5C) methylation potential modification sites were evaluated. Additionally, APC mRNA m5C methylation level and stability, the binding of APC mRNA with TRDMT1, the binding intensity of APC and β-catenin, and β-catenin nuclear translocation were detected Lastly, Cyclin D1, cellular-myelocytomatosis viral oncogene (C-myc) and β-catenin protein levels, and ki67-positive rate were assessed.ResultsSevo treatment boosted cell cycle, proliferation, migration and invasion, suppressed apoptosis and APC expression, and up-regulated C-myc, β-catenin, TRDMT1 and Cyclin D1 levels. Silencing TRDMT1 or β-catenin partially averted Sevo-mediated promotion effects on cell malignant biological behaviors. Lowly-expressed APC annulled the effect of silencing TRDMT1 and promoted cell malignant behaviors. Sevo enhanced APC mRNA m5C modification and degradation and activated the APC/β-catenin pathway by increasing TRDMT1, thus encouraging OC growth in vivo.ConclusionsSevo stimulated APC m5C modification and curbed its expression by up-regulating TRDMT1, which in turn activated the β-catenin pathway to stimulate OC cell cycle, invasion, proliferation, and migration and to suppress apoptosis.Graphical abstract

Elbow arthritis

Most patients with primary osteoarthritis of the elbow report a history of heavy lifting work with the affected upper limb. Conservative treatment, including activity modifications, nonsteroidal anti-inflammatory drug (NSAID) intake, and intra-articular injections can provide sufficient pain relief in the early stages when used in combination. If surgery is required, many patients experience significant pain relief from joint-preserving procedures despite existing cartilage damage. Although open debridement procedures are effective, arthroscopic osteocapsular arthroplasty has become established as the preferred surgical procedure. The ulnar nerve should be assessed during surgery and treated if necessary. Total elbow arthroplasty (TEP) is successful in terms of pain relief and function; however, it is recommended for older patients with advanced osteoarthritis or for whom joint-preserving procedures have failed.

Utilization of Metal-Functionalized ZSM-5 for Methanol and Low-Carbon Hydrocarbon Coupling Aromatization

Aromatics assume a paramount role as indispensable organic chemical feedstock within diverse industrial domains. Simultaneously, the global aromatics market is scarce, particularly with the exorbitant demand for high-value aromatics. Generating aromatics via coal-based methanol and low-carbon hydrocarbon coupling reactions has become a novel green and sustainable development trajectory. In this study, HZSM-5 catalysts featuring different Si/Al ratios and active metal-functionalized modifications were utilized to explore the aromatization effect in light of the Si/Al ratio, types of active components, and metal-loading content in a fixed-bed reactor. The outcomes were that the conversion ratios for methanol and n-pentane attained 99.9% and 83.1%, respectively. Remarkably, an oil phase yield of 32.1% was accomplished, along with an aromatic content of approximately 74.2%, while xylene selectivity reached approximately 37.6% for the 1.0%-ZnO/ZSM-5 (50) catalyst. Ultimately, a reaction mechanism for the coupling of methanol and n-pentane to yield aromatics using a 1.0%-ZnO/ZSM-5(50) catalyst is postulated.

Role of glutaminyl-peptide cyclo-transferase-like protein (QPCTL) in cancer: From molecular mechanisms to immunotherapy.

Glutaminyl-peptide cyclotransferase-like protein (QPCTL) is a newly discovered enzyme that has sparked interest owing to its possible role in cancer genesis and progression. Initially discovered as a post-translational modification regulator of protein maturation, QPCTL has emerged as a key participant in cancer biology. Recent research has linked QPCTL to numerous essential cancer-related processes, including cell proliferation, migration, invasion, and apoptosis. Furthermore, QPCTL expression changes have been seen in a variety of cancer types, underlining its potential as a diagnostic and prognostic marker. The molecular mechanisms behind QPCTL's participation in cancer will be examined in this review. We investigate its involvement in the control of signaling pathways and the modification of cellular activities that are important in cancer. We also examine the clinical importance of QPCTL, including as its relationship with tumor development, metastasis, and response to treatment. We also discuss the possible therapeutic implications of targeting QPCTL in cancer therapy. QPCTL is a prospective target for the development of innovative anticancer treatments due to its participation in several cancer-associated pathways.

A multifunctional coconut shell biochar modified by titanium dioxide for heavy metal removal in water/soil and tetracycline degradation

Heavy metals and tetracycline pollute the environment and endanger human health. This work prepared a multifunctional coconut shell biochar (Ti-XBC) through NaHCO3 activation and TiO2 modification. The modified coconut shell biochar was characterized by various methods. Ti-4BC exhibited excellent removal performance for heavy metals. The maximum adsorption capacity of Ti-4BC on Cd2+, Pb2+, Cr3+, and Cr6+ reached 104.2, 136.8, 101.2, 112.4 mg/g in water. The adsorption of Ti-4BC for four metal ions had a competitive effect in the binary metal system. It also reduced the mobility of four heavy metal ions in soil. Density functional theory (DFT) calculations were employed to explain the mechanism of heavy metal removal. Additionally, the degradation performance and mechanism of Ti-4BC towards tetracycline were investigated. The results indicated that the maximum degradation rate was 99.4%. Overall, this work developed a multifunctional material capable of both removing heavy metals and degrading antibiotics, addressing the issue of single-function in biochar. This provided a novel approach for tackling complex and diverse environmental pollution scenarios.

Expression, characterization and application of the deoxynivalenol dehydrogenase from Devosia yakushimensis.

Deoxynivalenol (DON) is one of the type-B trichothecene mycotoxins generally produced by Fusarium fungi and widely pollutes wheat and other grains, causing a great loss in grain yield and significant threat to human health. Enzymatic treatment of DON has displayed a significant role in controlling its levels in recent years. Here, the DON dehydrogenase from Devosia yakushimensis (DeDDH) is reported. DeDDH exhibited the highest activity in an environment of 35 °C and pH 7.5. In addition, the residual activity of DeDDH was about 40% after pre-incubation at 80 °C for 0.5 h, suggesting a good thermostability. The presence of Ca2+ significantly boosted the activity of DeDDH, but substituting Ca2+ with other ions could hardly retain the activity. At the optimal conditions, DeDDH efficiently degraded 75% DON (45 μg mL-1) within 4 h. Notably, when three cofactors, PQQ, PMS and DCPIP, were added together, more than 90% DON (45 μg mL-1) was quickly degraded in the first 40 min. The residues Thr485 and Leu521 were found to be significant to the activity of DeDDH by site-directed mutagenesis, much different from other DON dehydrogenases. Nevertheless, further research on the crystal structure determination of DeDDH, key catalytic residue identification, catalytic activity and thermostability modification still needs to be conducted for application in industry. © 2024 Society of Chemical Industry.

Research on the promotion effect of personalized training program based on biomechanics on the physical health of college students

Biomechanics-based personalized training programs can significantly improve physical health among college students, but there is limited evidence on how these programs affect spinal twist stretch function and overall health, despite previous research demonstrating the potential benefits of such programs. In this study, the influence of a biomechanics-based personalized training program investigates the physical health of a college student population. This study involved 68 college students, divided into two groups: 34 participants undergoing a personalized biomechanics-based training program (Group A) and 34 participants following a standard exercise regimen (Group B). The training program included exercises designed to enhance spinal twist stretch activation and stability. The participants’ core muscle activation was recorded through EMG from rhomboids, latissimus dorsi, and external obliques, while kinematic data were obtained from a three-dimensional motion analysis system. The assessments were done pre and post-8-week structured nutrition and physical activity modification period. These data are stimulated using SPSS, to compare pre and post-test measurements within each group and to determine the differences between spinal twist stretch and global physical health gains using paired t-tests, analyze of variance (ANOVA) descriptive statistics, and correlation analysis. The result demonstrated that Group A significantly improved spinal twists, stretch, and physical health among college students. Exercises targeting specific biomechanical principles exhibited more pronounced spinal twist stretch activation. The program improved trunk stability and movement accuracy, with a moderate correlation between muscle activation and overall physical health improvements. This approach improved core stability and muscle function but also led to better overall health outcomes compared to standard exercise regimens.

Preparation and salt storage sustained release performance of new ice suppression materials based on magnesium oxychloride cement

The poor salt storage and sustained release properties of active ice suppression materials for asphalt pavement has led to limitation in their application. In order to improve salt storage capacity and sustained release performance of ice suppression materials used in asphalt mixture, new salt storage carrier, magnesium oxychloride cement (MOC), was designed to prepare salt sustained release ice suppression materials in this work. Fly ash (FA) was adopted as a water resistance modifier for MOC after alkali activation, salt storage and hexadecyltrimethoxysilane (HDTMS) hydrophobic modification operations. The salt sustained release and ice suppression performance, microscopic morphology, hydrophobicity, water resistance of the ice suppression materials were evaluated by electrical conductivity, scanning electron microscope (SEM), contact angle, compressive strength and freezing point drop test. The results show that MOC is suitable for asphalt mixture pavement while ensuring the ice suppression performance, when the molar ratio of MgO, MgCl2 and H2O is 5.5: 1: 15. Alkali activation and HDTMS hydrophobic modification operations can significantly improve the salt storage and hydrophobic performance of FA. The water resistance and sustained release performance of MOC are enhanced, when FA is incorporated. The new ice suppression materials have better salt storage sustained release performance and the ice suppression effects are excellent, based on MOC as the carrier. It has certain significance in the field of active ice suppression in asphalt pavement engineering.

Augmented epigenetic repression of hepatitis B virus covalently closed circular DNA by interferon-α and small-interfering RNA synergy.

Since there are currently no approved drugs targeting and silencing covalently closed circular DNA (cccDNA), achieving a "functional cure" remains difficult. This study aims to comprehensively compare the effects of IFNα, small-interfering RNA targeting hepatitis B virus (HBV), and their combination on the activity, accessibility, and epigenetic modifications of cccDNA minichromosomes in cell models. A more durable and stable inhibition of HBV RNAs and antigens expression by IFNα and HBx-targeting siRNA (siHBx) synergy was observed, associated with augmented epigenetic repression of the cccDNA minichromosome. Besides, in an extracellular humanized IFNAR mouse model harboring recombinant cccDNA with an intact response to human IFNα, the synergistic effect of clinically used pegylated IFNα2 and in-house-developed GalNac-siHBx was further clarified.

Novel Therapies for Nonalcoholic Steatohepatitis (NASH) and Cardiovascular Risk Reduction.

Nonalcoholic steatohepatitis (NASH) is a type of nonalcoholic fatty liver disease (NAFLD) characterized by hepatocyte injury and inflammation, in addition to only the presence of steatosis NAFLD. We review the existing data on available novel therapies for NASH and NAFLD and also discuss several therapies in development. We assessed therapies for NASH by searching the databases of PubMed, EMBASE, and Web of Science (SCI) from their inception dates until September 15, 2024. Search terms used were: nonalcoholic fatty liver disease, nonalcoholic steatohepatitis, liver inflammation and hepatocyte injury.Until very recently, therapeutic lifestyle change was the primary modality of treatment for NASH, including modification of diet and physical activity. The FDA recently approved resmetirom using its expedited approval mechanism for NASH. There are also several pharmacotherapies in development for NASH which aim at weight loss, insulin sensitization and improvement in lipid levels, although some drugs may have multiple effects which are discussed. The availability of resmetirom offers patients with NASH an effective adjunctive therapy in addition to lifestyle changes. Several other novel therapies are also currently being tested and will add to our therapeutic armamentarium.

Relationship between trajectory of Chinese visceral adiposity index and risk of type 2 diabetes mellitus: Evidence from the China-PAR project.

This study aimed to identify the distinct change trajectories of the Chinese visceral adiposity index (CVAI) over time and to investigate their associations with risk of type 2 diabetes mellitus (T2DM). This study included 52 394 participants from the prospective project, the Prediction for Atherosclerotic Cardiovascular Disease Risk in China (China-PAR). The CVAI was calculated using measures of age, body mass index, waist circumference, triglycerides and high-density lipoprotein cholesterol. Latent mixture modelling was conducted to fit distinct trajectory patterns. The logistic regression model was applied to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the risk of T2DM with various CVAI trajectory patterns. Four distinct CVAI trajectory patterns were identified: low-increasing, moderate-increasing, moderate high-increasing and high-increasing. Compared with low-increasing CVAI, participants with moderate-increasing (OR 1.73, 95% CI 1.49-2.00), moderate high-increasing (3.48, 3.01-4.03) and high-increasing CVAI (5.50, 4.67-6.47) had a significantly increased risk of T2DM. Similar trajectory patterns were identified in both men and women. The ORs (95% CI) for moderate-increasing, moderate high-increasing and high-increasing groups were 3.28 (2.56-4.19), 7.85 (6.09-10.13) and 13.21 (9.98-17.49) in women respectively, and 1.20 (0.99-1.45), 2.18 (1.82-2.62) and 3.60 (2.93-4.43) in men respectively, when compared to the low-increasing CVAI group. Further, significant effect modifications for age, smoking and physical activity (all Pinteraction <0.05) were observed in the relationship between CVAI trajectory patterns and T2DM. Initially high and persistently elevated CVAI is significantly associated with an increased risk of T2DM, with a particular focus on women, younger people, nonsmokers and physically inactive individuals. Continuous monitoring of CVAI levels will benefit effective identification, early intervention and management of individuals at high risk of T2DM.

Impact of Immunosenescence on Vaccine Immune Responses and Countermeasures.

The biological progression of aging encompasses complex physiological processes. As individuals grow older, their physiological functions gradually decline, including compromised immune responses, leading to immunosenescence. Immunosenescence significantly elevates disease susceptibility and severity in older populations while concurrently compromising vaccine-induced immune responses. This comprehensive review aims to elucidate the implications of immunosenescence for vaccine-induced immunity and facilitate the development of optimized vaccination strategies for geriatric populations, with specific focus on COVID-19, influenza, pneumococcal, herpes zoster, and respiratory syncytial virus (RSV) vaccines. This review further elucidates the relationship between immunosenescence and vaccine-induced immunity. This review presents a systematic evaluation of intervention strategies designed to enhance vaccine responses in older populations, encompassing adjuvant utilization, antigen doses, vaccination frequency modification, inflammatory response modulation, and lifestyle interventions, including physical activity and nutritional modifications. These strategies are explored for their potential to improve current vaccine efficacy and inform the development of next-generation vaccines for geriatric populations.

Hydroxyl-Induced Modification of Oxygen Activation and Desorption Free Energy on Defective Tetragonal Zirconia Catalysts

Hydroxyl-Induced Modification of Oxygen Activation and Desorption Free Energy on Defective Tetragonal Zirconia Catalysts

Outcomes of Conservative Treatments Evaluations for Lumbar Radiculopathy

Background: Lumbar Radiculopathy (LR) is a condition characterized by motor, reflex and sensory changes, often causing significant pain and disability. Conservative treatments are commonly recommended as initial management options. This study aims to evaluate the outcomes of various conservative treatments for LR. Materials and methods: This prospective cohort study involved 100 patients diagnosed with LR who underwent conservative treatments between January 2023 and December 2023 at our Physical Medicine Department, Institute of Applied Health Sciences (IAHS). Treatments included physical therapy, NSAIDs, epidural steroid injections and activity modification. Baseline data, including demographic information, pain intensity (VAS) functional status (ODI) and quality of life (SF-36), were collected. Follow-up assessments were conducted at 6 weeks, 3 months and 6 months. Primary outcomes were changes in VAS, ODI and SF-36 scores. Secondary outcomes included patient satisfaction and the need for additional interventions. Data were analyzed using descriptive statistics, paired t-tests and ANOVA. Results: The mean age of participants was 45.3 years, with a slight male predominance (56%). At baseline, mean VAS was 7.8, ODI was 45.6 and SF-36 was 62.5. Significant reductions in VAS (2.6), ODI (18.7), and improvements in SF-36 physical (80.3) and mental (81.7) scores were observed at 6 months (p&lt;0.05). Patient satisfaction at 6 months was highest for epidural injections (90%), followed by NSAIDs (85%), physical therapy (80%) and activity modification (70%). The need for additional interventions decreased to 8%. The incidence of adverse events was low, with physical therapy showing the lowest rates. Initial ODI score was a significant predictor of positive outcomes (p=0.03). Conclusion: Conservative treatments for LR are effective in reducing pain and disability and improving quality of life. All treatment modalities showed comparable efficacy, with low adverse event rates, supporting their use as first-line treatments. Initial disability levels should be considered in treatment planning to optimize outcomes. Further research is needed to refine predictive models and enhance individualized treatment strategies. IAHS Medical Journal Vol 7(1), June 2024; 80-85

Abstract 4137145: Aortic Stenosis in Pregnancy: A Multidisciplinary Team Approach

Introduction: The hemodynamic adaptations that occur during pregnancy can have significant effects on patients with aortic stenosis (AS). These changes can lead to structural changes identified on echocardiogram even during uncomplicated pregnancies. While some cases can be managed with medical therapy and activity modification, others may require valve intervention in the gestational period. Case: A 29-year-old female with history of congenital bicuspid aortic valve, G1P0 presented at 42+2 weeks of pregnancy and was admitted to cardiac intensive care unit for continuous hemodynamic monitoring via use of arterial line. Her prior aortic valve disease was treated by valve repair with valvotomy, removal of tissue from pseudo raphe, removal of calcified scar tissue from both commissures, and a mitral valve repair for closure of an iatrogenic tear in her anterior leaflet. She followed closely with maternal fetal medicine and cardiology but became symptomatic at week 28 with lightheadedness, shortness of breath and decreased exercise tolerance (NYHA Class II). By week 33 she noticed lower extremity edema. Despite recommendations for planned delivery, she opted to wait until full term for delivery. Surveillance echoes revealed rising velocities yet stable valve area by planimetry. She ultimately underwent an uncomplicated Cesarean delivery of baby boy in week 42. At 6 months post partum she had successful transcatheter aortic valve replacement. Discussion: As the severity of valvular disease worsens there is a higher risk of complications during pregnancy. In our case, aortic valve velocities were elevated due to the expected hemodynamic changes of pregnancy. Thus, echocardiography monitoring should be used to assess the left ventricle’s ability to tolerate and compensate for these changes, while intervening based on symptoms and NYHA classification. We highlight the importance of multidisciplinary collaboration for safe and successful delivery in patients with AS.

The impact of androgen-induced translation in modulating androgen receptor activity

IntroductionDysregulated androgen receptor (AR) activity is central to various diseases, particularly prostate cancer (PCa), in which it drives tumour initiation and progression. Consequently, antagonising AR activity via anti-androgens is an indispensable treatment option for metastatic PCa. However, despite initial tumour remission, drug resistance occurs. Therefore, the AR signalling pathway has been intensively investigated. However, the role of AR protein stability in AR signalling and therapy resistance has not yet been deciphered. Therefore, this study aimed to investigate the role of AR protein changes in transactivity and assess its mechanism as a possible target in PCa.MethodsLNCaP, C4-2, and 22Rv1 cells were treated with R1881, enzalutamide, cycloheximide, and Rocaglamide. Mass spectrometry analyses were performed on LNCaP cells to identify the pathways enriched by the treatments. Western blotting was performed to investigate AR protein levels and localisation changes. Changes in AR transactivity were determined by qPCR.ResultsMass spectrometry analyses were performed on LNCaP cells to decipher the molecular mechanisms underlying androgen- and antiandrogen-induced alterations in the AR protein. Pathway analysis revealed the enrichment of proteins involved in different pathways that regulate translation. Translational and proteasome inhibitor experiments revealed that these AR protein changes were attributable to modifications in translational activity. Interestingly, the effects on AR protein levels in castration-resistant PCa (CRPC) cells C4-2 or enzalutamide-resistant cells 22Rv1 were less prominent and non-existent. This outcome was similarly observed in the alteration of AR transactivation, which was suppressed in hormone-sensitive prostate cancer (HSPC) LNCaP cells by translational inhibition, akin to the effect of enzalutamide. In contrast, treatment-resistant cell lines showed only a slight change in AR transcription.ConclusionThis study suggests that in HSPC, AR activation triggers a signalling cascade that increases AR protein levels by enhancing its translation rate, thereby amplifying AR activity. However, this mechanism appears to be dysregulated in castration-resistant PCa cells.

MobiChIP: a compatible library construction method of single-cell ChIP-seq based droplets.

To illustrate epigenetic heterogeneity, versatile tools of single-cell ChIP-seq (scChIP-seq) are essential for both convenience and accuracy. We developed MobiChIP, a compatible ChIP-seq library construction method based on current sequencing platforms for single-cell applications. MobiChIP efficiently captures fragments from tagmented nuclei across various species and allows sample mixing from different tissues or species. This strategy offers robust nucleosome amplification and flexible sequencing without customized primers. MobiChIP reveals regulatory landscapes of chromatin with active (H3K27ac) and repressive (H3K27me3) histone modification in peripheral blood mononuclear cells (PBMCs) and accurately identifies epigenetic repression of the Hox gene cluster, outperforming ATAC-seq. Meanwhile, we also integrated scChIP-seq with scRNA-seq to further illustrate cellular genetic and epigenetic heterogeneity.

Comprehensive Understanding of Laboratory Evolution for Food Enzymes: From Design to Screening Innovations.

In the field of food processing, enzymes play a pivotal role in improving product quality and flavor, and extending shelf life. However, the exposure of traditional food enzymes to high temperatures during processing often leads to a decrease in activity or even inactivation, limiting the effectiveness of their application under high-temperature conditions. Therefore, the modification of thermostability and activity of enzymes to adapt to extreme conditions through protein engineering has become a key way to improve the efficiency and economic benefits of industrial production. Directed evolution and semirational design strategies in the laboratory have proven to be broadly applicable frameworks for biochemical researchers in the food field, including those who are beginners. In this review, we systematically summarize semirational design strategies and high-throughput screening strategies, and introduce some intuitive computer simulation software to improve the thermostability and enzyme activity of food enzymes. The application of these strategies and techniques provides a comprehensive guide for the optimization of food enzymes. In addition, the latest hot topics of genetically engineered food enzymes in the field of application are discussed.

The Impact of the COVID-19 Pandemic on Waist and Hip Circumference and Selected Indicators Related to It Among Adolescents From the Kraków (Poland) Population.

The aim of the study was to assess changes in waist and hip circumference, as well as the waist-to-hip ratio (WHR) and waist-to-height ratio (WHtR) indices among adolescents (11-15-year-olds) from the Kraków during the COVID-19 pandemic. Cross-sectional studies were conducted in four main districts of the city in the years 2020 and 2022. The study group consisted of 1662 people. Measurements of height, waist circumference, and hip circumference were taken. The collected data were used to calculate the WHR and WHtR indices. Statistical analyses were performed using two-way ANOVA and post hoc Tukey's test, as well as the Kruskal-Wallis test, depending on the normality of distribution. An increase in hip circumference was observed in most age groups of both sexes. Among 14-15-year-old boys, an increase in waist circumference was observed, while among girls, the majority of cohorts showed the opposite trend. Additionally, in most age groups, a decrease in the values of WHR and WHtR indices was observed in both sexes, except for boys aged 14-15. The study found that government-imposed restrictions related to the COVID-19 pandemic had an impact on waist circumference, hip circumference, WHR, and WHtR indicators among adolescents aged 11-15 from Kraków. While some results showed regression, suggesting potential factors such as reduced physical activity or increased screen time contributing to deteriorating outcomes. On the other hand, not all results changed, which may be attributed to the absence of modifications in dietary habits or physical activity as government restrictions ceased.