Ml Of Dose Research Articles

Inmunocastración con Vacunación en Contra del Factor Liberador de Gonadotropinas (GnRF) Sobre el Comportamiento Productivo de Carnerillos

The objective of this study was to investigate the immunocastration using against gonadotropin-releasing factor (GnRF) with Bopriva® (Zoetis) on the performance of production variables, meat quality, docility, and scrotal circumference in young rams, from Sheep production center “Tupac Amaru”, Junin region, Perú. A total of 60 animals were divided into four treatments: T1, T2, and T3 with 0.50 mL, 0.75 mL, and 1.0 mL of doses with Bopriva®, respectively, and T4 (control - 1.0 mL of placebo (distilled water)), with fifteen animals in each. The variables were measured every 15 days for three months (September to November 2018). Datasets were assessed by one-way ANOVA and subsequent posthoc Tukey´s test to detect significant differences among treatments. The results indicated differences found between treatments with the immunocastration compared with non-castrated animals for final weight, carcass weight and performance, scrotal circumference, and docility. Growth performance and meat quality characteristics were not adversely affected by immunocastration. Likewise, immunocastration with Bopriva proved to be effective to stop scrotal development and consequently reduces the sexual and aggressive behavior of young rams. The dose of 0.5 mL seems to be adequate due showed better performance for most variables compared to other treatments

Optimization of curcumin loaded vaginal in-situ hydrogel by box-behnken statistical design for contraception

Sexually transmitted infections and unplanned pregnancies present a great risk to the reproductive health of women. Therefore, female-controlled vaginal products directed toward disease prevention and contraception is needed urgently. The purpose of this investigation was to develop poloxamer based thermo sensitive contraceptive vaginal in situ hydrogel of curcumin, a plant-derived diferuloylmethane compound. Different formulations using different ratio of poloxamer 407/188 and mucoadhesive polymer - HPMC K4M were prepared and optimized by box behnken experimental design (BBD). Formulations were optimized on the basis of gelation temperature (°C), gel strength (gm/cm2), Mucoadhesive strength (dyne/cm2), viscosity (Pa) and % drug release. Optimized formulation was subjected to In Vitro sperm immobilization study on 5 fertile human volunteers’ ejaculate sample. The quantitative effect of independent variables on dependent variables at different levels could be predicted by polynomial equations. From BBD, that batch containing 19.96% Poloxamer 407, 3.83% Poloxamer 188, 0.91% HPMC K4M was optimized. Total 100% Sperm immobilization was achieved within 15 min by optimized formulation for 3.5 ml of dose. Stability study of optimized batch shows no change in physical and chemical characteristics. Curcumin would be successfully formulated as thermo sensitive In Situ gelling mucoadhesive system for contraception.