Ml Of Decoction Research Articles

Phytochemical Variation of a Poly-herbal Formula According to Its Preparation Method: Qualitative & Quantitative Analysis

Madhyama Rasnadi decoction, one of the poly-herbal decoctions used in Ayurveda medicine is especially indicated for inflammatory conditions. The literature provides three different preparation methods of this decoction viz; Sri Lankan Traditional method (Method 1) and methods described in the texts Sharangadhara Samhita (Method 2) and Bhaishajya Ratnavali (Method 3). The aim of this study was to analyse and compare the phytochemical profiles of these three preparation techniques. Phytochemical profile analysis was carried out by (i) investigation of the extractable matter in 1 ml of decoction, (ii) development of Thin Layer Chromatography profiles and (iii) qualitative/quantitative determination of major phytoconstituents. Results revealed that the extractable matter of methods 1, 2 and 3 was 310±0 mg/ml, 420±0 mg/ml and 180±0 mg/ml, respectively. Differences (in terms of the number of spots and intensity) were observed in TLC fingerprint profiles, and phytochemicals such as phenols, tannins, flavonoids, saponins, alkaloids and terpenoids were present in all three types of decoctions. Gallic acid and quercetin were taken as standards to express the results of polyphenolic and flavonoid contents, respectively. Total polyphenolic contents of decoctions prepared according to methods 1, 2 and 3 were 121.68±0.60, 178.40±0.56, 86.20±0.25 mg gallic acid equivalents/g extract respectively. Total flavonoid contents of decoctions prepared according to methods 1, 2 and 3 were 69.45± 0.80, 129.30±0.65, 52.64± 0.50 mg quercetin acid equivalents/g extract respectively. In conclusion, more phytochemicals are concentrated on the decoction that made of method 2. The study opens more vistas of clinical applicability of Madhyama Rasnadi decoction, where further randomised case-control studies are needed.

Antiglycation and Antioxidant Activities of a Ready to Serve Herbal Drink of Syzygium Cumini Bark Extract

Diabetes mellitus is a major public health issue in most countries around the world. About 60% of the world’s population use herbal medicines as a remedy to prevent complications which arise due to glycation of proteins, enhanced by free radical formation. A previous study has proven the antiglycation and antioxidant potentials of Syzygium cumini decoction. The present study attempted to produce a ready to serve herbal drink using a water extract of Syzygium cumini bark. The consumer acceptable formulation selected by a sensory evaluation was investigated for the physical characteristics, microbial stability and antiglycation and antioxidant potentials up to a three months storage period. Antiglycation and antioxidant potentials were determined by Bovian Serum Albumin assay, ABTS and DPPH assay respectively. The overall acceptability of herbal formulation of 20 ml of decoction and 80 ml of 0.01% sucralose solution was optimum. Antiglycation activity of the freshly prepared drink was 35.8 μg/ml and did not decrease significantly during storage of 45 (39.2 μg/ml) and 90 days (41.1 μg/ml). The antioxidant potentials of fresh drink, during storage on day 45 and 90 days were 1314, 1200 and 1095 mmol/g TEAC and 82.3, 84.8 and 87.0 μg/ml for ABTS+ and DPPHI‡ assays respectively. The herbal drink was microbiologically safe up to three months under refrigerated conditions and physical characteristics did not change significantly. It is concluded that S. cumini decoction could be used as a valuable ingredient for the production of an herbal drink with many health benefits.

Clinical study on the effect of decoction of Pavetta indica Linn. in treatment of Purishaja Krimi with special reference to Enterobius vermicularis infestation

Pavetta indica Linn. (Family: Rubiaceae; Sanskrit name: Papata) is 2-5 m tall, shrub or small tree with opposite branches and grows in the Asia - Pacific region including Sri Lanka. Purishaja Krimi is one of the worm infestations described in Ayurveda. Enterobius vermicularis is among the most common of worms affecting children and adults. E. vermicularis is considered as one type of Purishaja Krimi. Sri Lankan traditional and ayurvedic physicians use P. indica to treat different ailments including Purishaja Krimi (E. vermicularis) infestations successfully. Since no scientific studies have been undertaken to study these effects so far, the present clinical study was carried out to evaluate the effect of P. indica in treatment of E. vermicularis infestation. Fifty patients between age of 5 and 12 years (Group A and B) and 50 patients between 13 and 65 years (Group C and D) with symptoms of E. vermicularis infestations such as itching in the anal region, impaired appetite, abdominal pain, eructation, diarrhea or constipation and presence of ova in stools were selected. Two decoction of the trail drug with different concentration was prepared. Group A and Group B were treated with 60 ml of decoction 1 and 60 ml of placebo respectively, twice daily for 14 days. Group C and Group D were treated with 120 ml of decoction 2 and 120 ml of placebo respectively, twice daily for 14 days. Groups A and C showed complete or partial reduction of symptoms, that is; itching in the anal region, impaired appetite, abdominal pain, eructation, diarrhea and also ova of E. vermicularis were absent in stools after treatment with P. indica. Decoction of P. indica can be recommended as an effective treatment for Purishaja Krimi (E. vermicularis infestation).

EXTRACTION, IDENTIFICATION AND QUANTIFICATION OF THE POLYPHENOLS OF GREEN AND BLACK TUNISIAN TEA DECOCTIONS COMMERCIALIZED AS "GARDEN OF TEA"

The dried tea leaves of Camellia sinensis is a popular beverage commonly consumed throughout the world. This research was conducted to determine the decoction time effect on the total phenolic content of green and black tea commercialized as Garden of Tea decoction in comparison to their infusions. During the experiment period, green and black tea leaves (40 g/L of water) were cooked for 15, 30 and 60 minutes continuously and discontinuously. After recovering by adding 3v of cold acetone, followed by rota-evaporation at 40°C under vacuum, the remaining aqueous extracts were evaluated for their total phenolics using the Folin-Ciocalteu method. The green tea cooked for 15 min had the highest quantity of polyphenols (1388±52 mg Gallic acid equivalent (GAE)/100 ml of tea decoction); while for the black tea, the highest phenolic content (953±40 mg Gallic acid equivalent (GAE)/100 ml of tea decoction) was obtained when cooking continuously for 30 minutes. The antioxidant and antimicrobial activities were also investigated. The antioxidant activity was evaluated by ABTS method using Trolox as reference. The strongest properties corresponded to the extracts obtained from green tea cooked for 15 minutes with a 4975±40 mg equivalent Trolox/100 ml of decoction. The antimicrobial potential of the tea phenolic extracts against a range of microorganism strains showed that Staphylococcus aureus and Staphylococcus epidermidis were the most sensitive.

A clinical trial of Sri Lankan traditional decoction of Pitawakka Navaya in treatment of Kaphaja Shirsha Shoola (chronic sinusitis)

Authentic Ayurvedic texts mention eleven types of Shiro Roga (diseases in head). Kaphaja Shirsha Shoola, which occurs due to vitiation of Kapha and Vata Dosha, is one of them. Chronic sinusitis is a chronic inflammation of sinuses. It can be correlated with Kaphaja Shirsha Shoola. Decoction of Pitawakka Navaya consists of nine ingredients. They are Bhoomyamalaka, Haritaki, Bharangi, Chavya, Pippali, Salarka, Guduchi, Shunti and Maricha. These herbs, which pacify vitiated Vata Dosha and Kapha Dosha, are used by traditional physicians in Sri Lanka to treat Kaphaja Shirsha Shoola (chronic sinusitis). However, no scientific studies have been conducted to evaluate the efficacy of Pitawakka Navaya in treatment of Kaphaja Shirsha Shoola. Hence, this clinical study has been conducted to evaluate its efficacy.. Sixty patients suffering from Kaphaja Shirsha Shoola were selected and examined. These patients were randomly divided into two groups of thirty patients each. First group was treated with 120 ml of decoction of Pitawakka Navaya twice a day for fourteen days. The second group was treated with 120 ml of placebo twice a day for fourteen days. After treatment, most of the patients’ symptoms were completely or partially relieved. In less than 10% of patients, some symptoms were unchanged or aggravated. It is observed that traditional decoction Pitawakka Navaya is beneficial for Kaphaja Shirsha Shoola (chronic sinusitis).

Efficacy of Sri Lankan traditional decoction of Katuwelbatu Deduru Katukadi in treatment of Kaphaja Shira Shula (chronic sinusitis)

Kaphaja Shira Shula is one of the Shiro Roga. Chronic sinusitis is inflammation of the sinuses behind the forehead, cheeks, and eyes, which continues for a long time or keeps recurring. Kaphaja Shira Shula can be correlated with Chronic Sinusitis. The decoction of Katuwelbatu Deduru Katuka is mentioned in Watika Prakaranaya, a book on Sri Lankan Traditional Medicine. The ingredients of this decoction are Solanum xanthocarpum, Cuminum cyminum, Nigella sativa, Picroihiza kurrota and Clerodendrum serratum. But in this study Saussurea lappa is used instead of C. serratum with the experience of traditional physician, Weerasinghe. Aim of this study was to scientifically evaluate the efficacy of this decoction in Kaphaja Shira Shula. Eighty patients suffering from Chronic sinusitis were selected from Ayurveda Teaching Hospital, Borella, Sri Lanka and randomly divided into two groups. X-ray of Para nasal sinuses and total white cell count before treatment and after treatment were recorded. Group one was treated with 120 ml of decoction of Katuwelbatu Deduru Katuka and Group two with 120 ml of placebo twice a day for twenty one days. Partial and complete symptomatic relief and reduction in esinophil count in the blood were observed in the treated group. It is observed that decoction of Katuwelbatu Deduru Katukadiya can be used in treatment of Kaphaja Shira Shula (Chronic sinusitis) effectively.

A clinical trial of Sri Lankan traditional decoction of Pitawakka Navaya in the treatment of Kaphaja Shirsha Shoola

Authentic Ayurvedic texts mention 11 types of Shiro Roga (diseases in the head). Kaphaja Shirsha Shoola, which occurs due to vitiation of Kapha and Vata Dosha, is one of them. Chronic sinusitis is a chronic inflammation of the sinuses. It can be correlated with Kaphaja Shirsha Shoola. Decoction of Pitawakka Navaya consists of nine ingredients; these are: Bhoomyamalaka, Haritaki, Barangi, Chavya, Pippali, Salarka, Guduchi, Shunthi and Maricha. These herbs, which pacify vitiated Vata Dosha and Kapha Dosha, are used by traditional physicians in Sri Lanka to treat Kaphaja Shirsha Shoola (chronic sinusitis). However, no scientific studies have been conducted to evaluate the efficacy of Pitawakka Navaya in the treatment of Kaphaja Shirsha Shoola. Hence, this clinical study was conducted to evaluate the efficacy of the decoction of Pitawakka Navaya in the treatment of Kaphaja Shirsha Shoola. Sixty patients suffering from Kaphaja Shirsha Shoola were selected and examined. These patients were randomly divided into two groups of 30 patients each. The first group was treated with 120 ml of decoction of Pitawakka Navaya twice a day for 14 days. The second group was treated with 120 ml of placebo twice a day for 14 days. After treatment, most of the patients’ symptoms were completely or partially relieved. In <10% of the patients, some symptoms were unchanged or aggravated. It is observed that the traditional decoction Pitawakka Navaya is beneficial for Kaphaja Shirsha Shoola (chronic sinusitis).