Background: This study was carried out to compare the rhizostimulation potentials of C. zizanioides and E. camaldulensis on microorganisms cfu during the phytoremediation of hydrocarbon contaminated potted soils. The objectives were to: i). determine the best treatment for the rhizostimulation of the microbial population in soils during the bioattenuation process. ii). examine the most efficient plant species in the Phytostimulation of hydrocarbon degrading microorganisms during the bioattenuation of hydrocarbon contaminated soils. iii). identify the various bacterial and fungal colonies associated with the bioattenuation of hydrocarbon. Materials and Method: The design was the Split-Split Plot experiment (4 x 4 x 2). The main plot factors were the crude oil contamination (4 levels), the sub-Plot factors were the soil amendments (4 levels) while the subsub-Plot factors were the plant species (2 levels). Results: Results indicated that there were significant differences (p < 0.05) in rhizostimulation potentials of the plant species in different crude oil contamination levels and in the different soil amendments for both bacteria and fungi. Although E. camaldulensis species was involved in biostimulation of microbial cfu, C. zizanioides was found better efficient (16.13 x 106 soil bacteria cfu g g -1 at 9 WAT and 10.04 x 106 soil fungal cfu g g -1 at 12 WAT respectively. Microorganisms found involved in bioattenuation of hydrocarbon include Bascillus spp., Staphylococcus spp., and Streptococcus spp for bacteria and Aspergillus spp., Rhizopus spp., and Penicillium spp for fungi. The most frequently occurred genus of microorganisms were Bacillus and Rhizopus. Conclusion: In conclusion, C. zizanioides was recommended as efficient rhizostimulating plant species. It was concluded that the mixture of both the grass and woody plant species are required for optimum growth and stimulation of hydrocarbon degrading microorganisms during phytoremediation procedure in the field. Additionally, bacterial and fungal cfu was observed to have increased with increasing hydrocarbon concentration under the influence of the two plant species and the applied landfarming treatments of a mixture of NPK (g g-1) and Cowdung (3:1 v/v) fertilizer. The most frequently occurred genus of microorganisms were Bacillus and Rhizopus.
Read full abstract