Hyperspectral technology has proven to be an effective method for monitoring soil salt content (SSC). However, hyperspectral estimation capabilities are limited when the soil surface is partially vegetated. This work aimed to (1) quantify the influences of different fraction vegetation coverage (FVC) on SSC estimation by hyperspectra and (2) explore the potential for a non-negative matrix factorization algorithm (NMF) to reduce the influence of various FVCs. Nine levels of mixed hyperspectra were measured from simulated mixed scenes, which were performed by strictly controlling SSC and FVC in the laboratory. NMF was implemented to extract soil spectral signals from mixed hyperspectra. The NMF-extracted soil spectra were used to estimate SSC using partial least squares regression. Results indicate that SSC could be estimated based on the original mixed spectra within a 25.76% FVC (R2cv = 0.68, RMSEcv = 5.18 g·kg-1, RPD = 1.43). Compared with the mixed spectra, NMF extraction of soil spectrum improved the estimation accuracy. The NMF-extracted soil spectra from FVC below 63.55% of the mixed spectra provided acceptable estimation accuracies for SSC with the lowest results of determination of the estimation R2cv = 0.69, RMSEcv = 4.15 g·kg-1, and RPD = 1.8. Additionally, we proposed a strategy for the model performance investigation that combines spearman correlation analysis and model variable importance projection analysis. The NMF-extracted soil spectra retained the sensitive wavelengths that were significantly correlated with SSC and participated in the operation as important variables of the model.
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