Mixed-ligand Method Research Articles

Photocatalytic degradation of tetracycline hydrochloride and oxytetracycline using novel zinc-based coordination polymers constructed with mixed linkers

In the present work, a new zinc-based coordination polymer was successfully synthesized by mixed-ligand method with the molecular formula of {[Zn(ipa)(Hpap)]·2H2O}n, (abbreviated as Zn-CP), where ipa = isophthalic acid, Hpap = 4-(1H-pyrazol-4-yl)pyridine. Furthermore, reasonable characterization of Zn-CP was carried out by single crystal X-ray diffraction (SC-XRD), powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA) and other spectra. During the characterization process, it was found that Zn-CP can have excellent acid-base stability, water stability and thermal stability. In addition, the complex was able to effectively degrade tetracycline hydrochloride (TCH) and oxytetracycline (OTC) within 60 min under visible light irradiation (with degradation efficiencies of 80.2 % and 83.5 %, respectively), and the cycling experiments showed that Zn-CP could still effectively degrade TCH and OTC after three cycles. Finally, in order to propose a rational photocatalytic degradation mechanism, free radical trapping experiments and energy band structure analysis were also carried out.

Cu(II)-based complex loaded with drug paclitaxel hydrogels against thyroid cancer and optimizing novel derivatives

This study introduces a novel approach for synthesizing a Cu(II)-based coordination polymer (CP), {[Cu(L)(4,4´-OBA)]·H2O}n (1), using a mixed ligand method. The CP was successfully prepared by reacting Cu(NO3)2·3H2O with the ligand 3,6-bis(benzimidazol-1-yl)pyridazine in the presence of 4,4´-H2OBA, demonstrating an innovative synthesis strategy. Furthermore, a novel hydrogel composed of hyaluronic acid (HA) and carboxymethyl chitosan (CMCS) with a porous structure was developed for drug delivery purposes. This hydrogel facilitates the encapsulation of CP1, and enables the loading of paclitaxel onto the composite to form HA/CMCS-CP1@paclitaxel. In vitro cell experiments demonstrated the promising modulation of thyroid cancer biomarker genes S100A6 and ARID1A by HA/CMCS-CP1@paclitaxel. Finally, reinforcement learning simulations were employed to optimize novel metal–organic frameworks, underscoring the innovative contributions of this study.

Comparison of modulation strategies for enhancing the photocatalytic water splitting performance of metal-organic frameworks

Metal-organic framework materials (MOFs) have proven to be an excellent catalyst, but how to further improve their photocatalytic performance has become a challenge for researchers. In this study, we focus on the effect of two modulation strategies on the photocatalytic performance of MOFs, and 2-mercaptobenzimidazole (MBI) was introduced into the ZIF-67 structure by the solvent-assisted ligand exchange (SALE) method and the mixed ligand method, respectively, to modulate their photocatalytic performance. It was found that both strategies can successfully introduce MBI into the ZIF-67 structure, but the mixed ligand method tends to destroy the crystal morphology and structure of ZIF-67 and constructs a good deal of mesopores in the structure, which is detrimental to the improvement of catalytic performance. On the contrary, the SALE strategy can maintain the special crystal morphology and structure of ZIF-67 while introducing MBI, and can construct a suitable number of defects to enhance the photogenerated carrier transfer and separation efficiency of ZIF-67. Under the action of visible light, the ZIF-67- MBI catalysts obtained by the SALE method have the optimal photocatalytic activity, and their photocatalytic hydrogen and oxygen evolution rates can reach 6.14 mmol g−1 h−1 and 210 mmol g−1 h−1, respectively. This study demonstrates that the SALE method is a viable strategy to modulate the photocatalytic performance of MOFs and provides a reference for the construction of composites with excellent photocatalytic properties.

A water stable and highly fluorescent Zn(II) based metal-organic framework for fast detection of Hg2+, CrVI, and antibiotics.

The development of luminescent metal-organic frameworks for effective sensing and monitoring of environmental pollutants is of great significance for human health and environmental protection. In this work, a novel water-stable ZnII-based luminescent coordination polymer, namely {[Zn(BBDF)(ATP)]·2DMF·3H2O}n ((BBDF = 2,7-bis(1H-benzimidazol-1-yl)-9,9-dimethyl-9H-fluorene) and H2ATP = 2-aminoterephthalic acid), was designed and obtained using the mixed-ligand method. Structural analysis indicated that 1 presents a two-fold interpenetrated two-dimensional layer structure with one dimensional (1D) channels along the a axis. Intriguingly, the uncoordinated -NH2 group was danged onto the pore walls of 1. Remarkably, compound 1 shows good aqueous stability at different pH values of 3-13 and exhibits a fluorescence turn-off sensing behavior for Hg2+, Cr2O72-, CrO42-, and antibiotics (NFZ, NFT) in aqueous solution with high selectivity and sensitivity. The limits of detection (LOD) are 0.12 μM (Hg2+), 0.17 μM (Cr2O72-), 0.21 μM (CrO42-), 0.098 μM (NFZ), and 0.14 μM (NFT). The luminescence quenching mechanism analysis by experiment and theoretical calculation revealed that the competitive absorption and the photoinduced electron transfer process are largely responsible for the sensing of the two antibiotics, while the weak interaction contributes to the selective luminescence quenching for Hg2+.

New Mn(II)-coordination polymer based on flexible benzimidazol ligand: crystal structure and treatment on osteosarcoma

In this study, a Mn(II)-based coordination polymer with the chemical composition of {[Mn(L)(TBTA)(H 2 O) 2 ]·H 2 O} n ( 1 ) was prepared by reacting Mn(OAc) 2 ·2H 2 O with the flexible ligand 1,4-bis(5,6-dimethylbenzimidazol-1-yl)-2-butylene in the aid of the carboxylic acid ligand tetrabromoterephthalic acid (H 2 TBTA) through the mixed-ligand method. Its application values on osteosarcoma were measured, and the internal principle was discussed simultaneously. The inhibitory activity of the new compound on the multiplication of osteosarcoma cells was evaluated using the Cell Counting Kit-8 detection kit. The facilitation of the LKB1-mTORC1 signaling pathway was estimated via real-time RT-PCR.

Mixed-Ligand Uranyl Squarate Coordination Polymers: Structure Regulation and Redox Activity.

The electron-rich squarate ion (C4O42-, SA2-) possesses electronic delocalization over the entire molecule and good redox activity, and the functionalization of metal-organic complexes with the SA2- group is desirable. In this work, a mixed-ligand method is used to construct novel uranyl squarate coordination polymers utilizing 4,4'-bipyridine (bpy), 4,4'-bipyridine-N,N'-dioxide (bpydo), 1,10-phenanthroline (phen), 4,4'-vinylenedipyridine (vidpy), and in situ formed oxalate (OA2-) as ancillary ligands. Seven mixed-ligand uranyl compounds, [(UO2)(OH)(SA)](Hbpy) (1), [(UO2)(H2O)(SA)2](H2bpy) (2), (UO2)(H2O)(SA)(bpydo)·2H2O (3), (UO2)(H2O)(SA)(phen)·H2O (4), (UO2)(OH)(SA)0.5(phen)·H2O (5), [(UO2)(SA)(OA)0.5](Hphen) (6), and [(UO2)(SA)(OA)0.5](Hvidpy) (7), with varying crystal structures were synthesized under hydrothermal conditions. Compound 1, together with bpy molecules filling in the interlayer space as template agents, has a two-dimensional (2D) network structure, while 2 gives a one-dimensional (1D) chain based on mononuclear uranium units. Compound 3 shows a neutral 2D network through the combined linkage of SA2- and bpydo. Both 4 and 5 have a similar chain-like structure due to the capping effect of phen motifs, while phen molecules in 6 act as templating agents after protonation. Similar to 6, compound 7 has a "sandwich-like" structure in which the Hvidpy motifs locate in the voids of layers of 2D uranyl-squarate networks. The redox properties of typical mixed-ligand uranyl-squarate compounds, 1, 4, and 5 with high phase purity, are characterized using cyclic voltammetry. All three of these uranyl coordination compounds show anode peaks (Ea) at 0.777, 0.804, and 0.760 V, respectively, which correspond to the oxidation process of SA2- → SA. Meanwhile, cathodic peaks (Ec) at -0.328, -0.315, and -0.323 V corresponding to the reduction process of U(VI) → U(V) are also observed. The results reveal that all three of these uranyl coordination compounds show good redox activity and, most importantly, the interplay between two different redox-active motifs of SA2- organic linker and uranyl node. This work enriches the library of redox-active uranyl compounds and provides a feasible mixed-ligand method for regulating the synthesis of functional actinide compounds.

A luminescent Tb(III)-organic framework: Selective sensing of PO4 3– ions and important role on tuberculosis curing

In the current study, via applying the mixed-ligand method, a new metal-organic framework (MOF) containing Tb was hydrothermally synthesized and characterized; its chemical formula is {[Tb(dpc)(H2O)2]·(Hbibp)0.5} n (1, in which bibp = 4,4′-bis(imidazolyl) biphenyl and H4dpc = 2-(3′,4′-dicarboxylphenoxy) isophthalic acid). The as-produced 1 reveals significant sensitivity and remarkable selectivity for the detection of PO4 3– as a kind of easy-to-use fluorescent probe having extensive range of detection, rapid response, and low limit of detection. The Mycobacterium tuberculosis bacterial burdens were measured with the spread plate method. In addition to this, real time RT-PCR was implemented for the evaluation of the survival genes relative expression levels in the Mycobacterium tuberculosis. The carboxyl-rich metal complex was further evaluated by molecular docking simulation which confirmed that multiple binding interactions were formed with active sites from the target protein.

Two New Coordination Polymers: Selective Detection of Cu(II) Ion and Treatment Activity on Inner Ear Damage.

In the current study, on the basis of 1,3,5-tris(2-methylimidazol-1-yl)benzene (timb), a designed tripodal connector, two new transition metal coordination polymers (CPs), {[Cu4(timb)2(Br-IPA)4]·5H2O}n (1) and {[Zn(timb)0.5(NH2-IPA)]·4H2O}n (2) have been generated with the mixed ligand method by the reaction between the timb and corresponding metal salts in the existence of dissimilar functional isophthalic acid (H2IPA) ligands. Furthermore, the Zn(II)-based complex 2 displays high sensitivity in the detection of Cu(II) ion in water. The neural stem cells proliferation after treated via compounds was detected with Cell Counting Kit-8 detection assay. And the real time reverse transcription polymerase chain reaction was carried out for the investigation of the differentiation function of the neural stem cells after the compound 1 treatment and compound 2 treatment. Further, molecular docking simulations confirmed that the biological activity that has been observed from experiments were from the carboxyl group on the Cu complex, in contrast, the imidazole groups were only used for binding with the Cu metal ion to retain the complex structure.

RETRACTED: Application of porous Zn(II)-organic framework for C2H2 separation and postmenopausal osteoarthritis treatment by regulating estrogen levels

Via exploiting the mixed-ligand method, a novel metal-organic framework (MOF) having Zn(II) (namely, {[Zn(HBmic)(2-Aim)]·3DMF·2H2O} (1, 2-Aim=2-Aminoimidazole, H2Bmic=1-benzimidazole-5-carboxylic acid)) has been successfully prepared. Furthermore, activated 1 can adsorb the C2H2 selectively over CH4 and CO2, revealing high CO2/C2H2 and CH4/C2H2 separation under the environmental conditions. The theoretical selectivity of desired adsorption solution is 4.7–12.4 and 43.4–64.6, respectively. For the treatment of postmenopausal osteoarthritis, the biological activity of the new compound against postmenopausal osteoarthritis was evaluated and the detail mechanism was explored at the same time. The possible binding interactions that are initiated by the Zn complex have been further confirmed by performing molecular docking simulations, where the explicit hydrogen bonds are identified.

Two Zn(II)-organic frameworks: catalytic Knoevenagel condensation and treatment activity on spine surgery incision infection via inhibiting Staphylococcus aureus biofilms formation

By utilizing the mixed-ligand method, two novel metal-organic frameworks (MOFs) based on Zn(II) ions as nodes with the chemical formulae of {[Zn2.5(abta)(trz)2(H2O)]·3H2O}n (1, Htrz = 1H-1,2,4-triazole) and [Zn3(abta)2(bibb)2]n (2, bibb = 1,4-bis(benzimidazol-1-yl)-2-butene) were produced via Zn(NO3)2·6H2O reacting with the 1-aminobenzene-3,4,5-tricarboxylic acid (H3abta) in the existence of distinct nitrogen-donor co-ligands. The different N-donor ligands result in their distinct framework structures, and the compound 1 with higher solvent accessible void and large window size shows highly heterogeneous catalytic activities for Knoevenagel condensation. The treatment of Staphylococcus aureus biofilms formation during spine surgery incision infection and the related mechanism was explored at the same time. First of all, the S. aureus bacterial numbers in the infectious site was measured under compound 1 or 2 treatment. In addition to this, the relative expression levels of the genes related with S. aureus biofilms was determined with real time RT-PCR.

RETRACTED ARTICLE: Two Cu(II) Coordination Polymers: Treatment Activity on Spine Surgery Incision Infection by Inhibiting the Staphylococcus aureus Biofilm Formation

Through applying the mixed-ligand method, two coordination polymers (CPs) containing Cu(II) ions as the metal centres have been synthesized, and their chemical formulas are [{Cu(muco)(bpa)(2H2O)}·2H2O]n (1) and [{Cu(muco)(4bpdp)}·2DMF·H2O]n (2) (in which, 4bpdb = 1,4-bis(4-pyridyl)-2,3-diaza-1,3-butadiene, bpa = 1,2-bis(4-pyridyl)ethane and the muco = trans, trans-muconate dianion). The treatment activity of compound 1 and the compound 2 was evaluated in the biological experiments. Firstly, the inflammatory response of the spine surgery incision was assessed by the enzyme linked immunosorbent assay (ELISA) detection. Besides, the inhibitory action of both two compounds against the Staphylococcus aureus biofilm formation was further detected utilizing the real time reverse transcription-polymerase chain reaction (RT-PCR).

Donor–Acceptor Polymers Containing 4,8-Dithienylbenzo[1,2-b:4,5-b′]dithiophene via Highly Selective Direct Arylation Polymerization

While palladium-catalyzed direct arylation polymerization (DArP) is an easy and safe method to synthesize semiconducting polymers with alternating donor–acceptor units (DA polymers), branching and cross-linking defects arising from unintended side reactions are common. When these polymers are used as electronic materials in organic solar cells (OSCs) and similar devices, these defects may adversely affect device performance. We have recently shown that the side reactions can be inhibited by the simultaneous presence of two ligands, P(2-MeOC6H4)3 and N,N,N′,N′-tetramethylethylenediamine (TMEDA). In this study, an efficient donor polymer for OSCs (P1), based on 4,8-dithienylbenzo[1,2-b:4,5-b′]dithiophene (DTBDT) units, was prepared with this mixed-ligand method. The DA polymer is found to exhibit a well-controlled structure, with homocoupling defects of 2.0%, compared to 14.8% observed in the Migita–Stille product (P2). The power conversion efficiency (PCE) was 9.9% when P1 was used, compared to 9.8% for P2 in bulk-heterojunction-type OSC with a nonfullerene acceptor. This demonstrates the effectiveness of the DArP method as a clean and safe alternative method to synthesize DTBDT-based DA polymers for OSCs.

RETRACTED: Two Zn(II) coordination polymers: Selective detection Fe3+ ion and inhibitory activity on the liver cancer viability by regulating the prolyl hydroxylase-3

Abstract In the current study, via utilizing a mixed-ligand method, two new coordination polymers (CPs) of {[Zn5(pmbcd)2(μ3-OH)2(H2O)4(DMF)2]·4DMF}n (1) and {[Zn2(pmbcd)(bpea)2]·2H2O·2DMF}n (2) (in which bpea = 1,2-bis(4-pyridyl)ethane and H4pmbcd = 9,9′-(1,4-phenylenebis(methylene))bis(9H-carbazole-3,6-dicarboxylic acid)) have been successfully prepared using a tetracarboxylate ligand H4pmbcd without or with the present of the second co-ligand bpea. Furthermore, in the water, the complex 1 containing Zn5 clusters exhibits the strong blue luminescence, which can only be selectively quenched via the contaminant Fe3+ ion. For the treatment of liver cancer, the Cell Counting Kit-8 assay was implemented for the assessment of the compounds’ inhibition on the HepG2 liver cancer cells viability. Moreover, the prolyl hydroxylase-3 relative expression in HepG2 liver cancer cells was also detected by the real time reverse transcription polymerase chain reaction.

A new Cu(II)-based coordination polymer: treatment activity on the glomerulonephritis via inhibiting the mesangial cell proliferation

Through applying the mixed-ligand method, a new coordination polymer (CP) of Cu(II) with the chemical formula of {Cu5(L)2(1,4-bimb)(μ3-OH)2(H2O)8]·2H2O}n (1, 1,4-bimb = 1,4-bis(imidazol-1-ylmethyl)benzene and H4L = 1,2,4,5-cyclohexanetetracarboxylic acid) was synthesized under the solvothermal conditions. Furthermore, the biological effect of compound against glomerulonephritis therapy was detected, and the details mechanism was investigated. First of all, the mesangial cells proliferation was detected through using the Cell Counting Kit-8 (CCK-8) method. Then, the Annexin V-FITC/PI method was utilized to determine cell cycle arrest and the apoptosis rate. Next, the caspase-8 and caspase-3 expression level in mesangial cells was detected via applying the western blot method. Finally, the inflammatory cytokines released into the plasma was assessed by ELISA detection kit.

A Mixed-ligand Pb(II)-coordination Polymer: Photocatalytic Performance and Reduction Activity of the Inflammatory Response After Severe Burns by Inhibiting NF-κB Signaling Pathway.

Via the employment of a mixed-ligand method, a novel Pb(II)-coordination polymer with the chemical formula of [Pb(pdc)(bdc)]·2DMF (1, H2bdc = 1,4-benzenedicarboxylic acid and H2pdc = 3,5-pyridinedicarboxylic acid) has been produced via reaction of Pb(NO3)2 with the two organic linkers in a mixed solvent of DMF and water. Complex 1 has high photocatalytic degradation efficiency for methylene blue and rhodamine B under ultraviolet light irradiation. The mechanism of photocatalysis was hypothesized and verified in detail by the r photocatalysis reaction in the existence of the hydroxyl radical scavenger mannitol. Furthermore, the influence of the inflammatory response in the severe burns rats was evaluated. Firstly, the inflammatory response levels were assessed by measuring the content of IL-6 and TNF-α in the plasma after treated with compound. Next, the real time RT-PCR was carried out to determine the signaling pathway of NF-κB relative expression in inflammatory cells.

Two coordination polymers: effect on the learning and memory impairment in epilepsy mice by regulating the mTOR signaling pathway

Under similar hydrothermal conditions, via using the mixed-ligand method, two 3-D coordination polymers (CPs) [Zn(HIPA)(bmimb)]n (1, bmimb = 1,4-bis(2-methyl-1H-imidazol-1-yl)benzene)) and [Cu2(HIPA)2 (bmib)3]n (2, bmib = 1,4-bis(1H-imidazol-1-yl)benzene) were fabricated based on the reaction of Zn(NO3)2·6H2O or Cu(NO3)2·3H2O with 5-(hydroxymethyl)isophthalic acid (H3IPA) when N-donor co-ligands were present. The compounds were evaluated to improve the learning and memory impairment in epilepsy mice and the relationship between the mTOR signaling pathway and epilepsy is discussed. To determine the influence of 1 and 2 on epilepsy mice cognitive function, the Morris Water Maze Test was performed. The mTOR signaling pathway expression levels were then detected with western blot.

RETRACTED: Two mixed-ligand coordination polymers: selective detection of acac and treatment activity on peripheral artery disease by reducing the peptidyl arginine deiminase activity

• Two coordination polymers were synthesized. • Complex 1 possesses the sensing activities with high sensitive and selective to the acetylacetone (acac) through a luminescence enhancement. • The treatment activity of compounds on the peripheral artery disease was further explored in the part of biology. Via utilizing a mixed-ligand method, two coordination polymers {[Cd(HL)(bpea)•H 2 O]•H 2 O•DMF} n ( 1 , bpea = 1,2-di(4-pyridyl)ethane) and {[Co(HL)(pbix)]•2H 2 O} n ( 2 , pbix = 1,4-bis(imidazol-1ylmethyl)benzene) with structural diversity were synthesized through corresponding metal salts react with 5-((4-carboxybenzyl)oxy)isophthalic acid (H 3 L), a flexible oxygen-joint ligand in the existence of distinct N-donor co-ligands. Luminescence measurements indicated that the Cd(II)-based complex 1 possesses the sensing activities with high sensitive and selective to the acetylacetone (acac) through a luminescence enhancement. The treatment activity of compounds on the peripheral artery disease was further explored in the part of biology. First of all, real time RT-PCR method was conducted and the expression levels of the peptidyl arginine deiminase were measured. Then, the ELISA test kit was applied and the inflammatory cytokines content was detected.

RETRACTED ARTICLE: A fluorescent coordination polymer for TNP detection and protection effect on cough after infection by up-regulates the activity of neuroendopeptide enzyme in respiratory tract mucosa

Through utilizing the mixed-ligand method, a novel coordination polymer based on Zn(II) ions as nodes was synthesized under hydrothermal condition via applying 1,4-cyclohexanedicarboxylic acid (1,4-H2chdc) and 2-(4-fluoro-phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline (L), a π-electron rich ligand, as co-ligand, and its chemical formula is [Zn(1,4-chdc)(L)(H2O)]n (1). Because of its strong ambient temperature fluorescent emission and outstanding water stability, the complex 1 could be utilized as the sensitive sensor for the 2, 4, 6-trinitrophenol (TNP) via fluorescence quenching, and the constant of quenching can reach 2.794 × 105 M−1. The following experiment was further conducted to detect the treatment activity of the synthetic compound against post-infectious cough. The release of inflammatory cytokines and endopeptidase from respiratory tract mucosa was determined through enzyme linked immunosorbent assay (ELISA). Next, the fluorescent probe of DCFH-DA was utilized to detect the accumulation of ROS in the respiratory mucosal epithelial cells.

RETRACTED: Two Cu(ii)-based coordination polymers: Dye degradation and important values on the later recovery of fracture patients by activating the WNT signaling pathway

• Two new Cu(II)-based coordination polymers were synthesized. • Compound 1 with 3-fold interpenetrated network shows high photocatalytic degradation efficiency toward MB dye. • The application values of two compounds on the later recovery of fracture patients were evaluated. Through utilizing the mixed-ligand method, two novel coordination polymers {[C u ( bpdc )( bpbenz )]} n ( 1 , H 2 bpdc = [1,1′-biphenyl]-4,4′-dicarboxylic acid, bpbenz = 1,4-bis(4-pyridyl)benzene) and {[C u ( bpdc )( bide ) 2 ]} n ( 2 , bide = 4,4′-bis(imidazolyl)diphenyl ether) with structural diversity have been synthesized via Cu(NO 3 ) 2 •3H 2 O reacting with the rigidity bicarboxylic ligands H 2 bpdc in the existence of the distinct nitrogen-donor co-ligands. The photocatalytic degradation performance of complex 1 to distinct dye contaminants (MO, RhB and MB) was assessed. The results confirm that the photocatalytic degradation efficiency of complex 1 to the MB is the highest (MO 86.2%, RhB 85.7% and MB 92.1%). Their application values on the later recovery of fracture patients were then evaluated. The real time RT-PCR method was conducted to assess the osterix and runx2 relative expression levels in the mesenchymal stem cells (MSCs). The western blot method was implemented and the WNT (WNT is a fusion of two terms, wg derived from the Drosophila gene wingless (wg) and int derived from the proto-oncogene integration, which is the mammalian homolog of wg) protein expression levels were assessed.

RETRACTED: Two Cu(II)-based coordination polymers: Enhancement on acupuncture treatment activity during salpingitis

• Two coordination polymers (CPs) were successfully synthesized. • The coordination polymers were characterized through single crystal diffraction of X-ray. • Compounds 1 and 2 were studied in our lab for the treatment of salpingitis combined with acupuncture. Via utilizing the mixed-ligand method, two coordination polymers (CPs) of { [Cu 2 (SDB) 2 (L 1 )]·(H 2 O) 4 ·(DMF)} n ( 1 ) and {[Cu(L 2 )(SDB)]} n ( 2 ) [L 2 = 4,4′-(2,5-bis(methylthio)-1,4-phenylene)dipyridine and the L 1 = 1,4-bis(4-pyridyl)-2,3-diaza-1,3-butadiene] were synthesized hydrothermally and then the coordination polymers were characterized through X-ray powder diffraction, IR, single crystal X-ray vdiffraction, and the measurements of thermogravimetry, as well as elemental analysis. Compounds 1 and 2 were studied in our lab for the treatment of salpingitis combined with acupuncture, the biological functional was evaluated and the mechanism was discussed. The inflammatory levels in the fallopian tube epithelial cells were measured through the ELISA test. Afterward, the JAK-STAT signaling pathway relative expression in the epithelial cells of fallopian tube was determined through the real time RT-PCR.