Mix Treatment Research Articles

Effect of Rhizobacteria Application on Nutrient Content, Bioactive Compounds, Antioxidant Activity, Color Properties and Fruit Characteristics of Strawberry Cultivars

The aim of this study was to determine the effects of single and combined applications of plant-growth-promoting rhizobacteria (PGPR) bacteria on plant nutrition, biochemical content and fruit characteristics in Albion and Monterey strawberry cultivars. Bacillus subtilis OSU-142, Bacillus megaterium M3 and Paenibacillus polymyx were the PGPR used in the experiment. For each bacterial treatment, 10 mL of a 108 CFU mL−1 suspension was applied to the soil where Albion and Monterey cultivars were grown. PGPR bacteria were applied as single treatments and a mixture of equal amounts of these three bacterial species was applied as a mixed treatment. This study was carried out with a total of four different bacterial treatments and one control group. The highest fruit weight was obtained in the Monterey cultivar with 12.67 g in the Mix treatment and in the Albion cultivar with 11.79 g in the Bacillus megaterium M3 treatment. Regarding biochemical properties, Paenibacillus polymyxa was effective in influencing nutrient element content in fruits, while Bacillus subtilis OSU-142, Paenibacillus polymyxa and Bacillus megaterium M3 applications were more effective in leaf nutrient element content. It has been observed that the Mix treatment resulting from the combined use of bacteria, rather than their separate use, has a greater impact on fruit weight. Consequently, it has been understood that PGPR bacteria are potentially effective in improving the agronomic, pomological, and biochemical characteristics of strawberry cultivars and can be used in studies and breeding programs aimed at increasing strawberry yield and quality.

Preventing social defeat stress-induced behavioural and neurochemical alterations by repeated treatment with a mix of Centella asiatica, Echinacea purpurea and Zingiber officinale standardized extracts.

Background: Prolonged exposure to stress is a risk factor for the onset of several disorders. Modern life is burdened by a pervasive prevalence of stress, which represents a major societal challenge requiring new therapeutic strategies. In this context, botanical drug-based therapies can have a paramount importance. Methods: Here we studied the preventive effects of a repeated treatment (p.o. daily, 3weeks) with a combination of Centella asiatica (200mg/kg), Echinacea purpurea (20mg/kg) and Zingiber officinale (150mg/kg) standardized extracts, on the chronic social defeat stress (CSDS) deleterious outcomes. After 10days of CSDS exposure, male mice' performances were evaluated in paradigms relevant for social (social interaction test), emotional (tail suspension test), cognitive (novel object recognition) domains as well as for pain perception (cold plate and von Frey tests) and motor skills (rotarod). Mice were then sacrificed, the spinal cords, hippocampi and frontal cortices dissected and processed for RT-PCR analysis. Results: Extracts mix treatment prevented stress-induced social aversion, memory impairment, mechanical and thermal allodynia and reduced behavioural despair independently of stress exposure. The treatment stimulated hippocampal and cortical BDNF and TrkB mRNA levels and counteracted stress-induced alterations in pro- (TNF-α, IL-1β and IL-6) and anti-inflammatory (IL4, IL10) cytokines expression in the same areas. It also modulated expression of pain related genes (GFAP and Slc1a3) in the spinal cord. Conclusion: The treatment with the extracts mix obtained from C. asiatica, E. purpurea and Z. officinale may represent a promising strategy to promote resilience and prevent the deleterious effects induced by extended exposure to psychosocial stress.

Cerebral Vascular Toxicity after Developmental Exposure to Arsenic (As) and Lead (Pb) Mixtures.

Arsenic (As) and lead (Pb) are environmental pollutants found in common sites linked to similar adverse health effects. This study determined driving factors of neurotoxicity on the developing cerebral vasculature with As and Pb mixture exposures. Cerebral vascular toxicity was evaluated at mixture concentrations of As and Pb representing human exposures levels (10 or 100 parts per billion; ppb; µg/L) in developing zebrafish by assessing behavior, morphology, and gene expression. In the visual motor response assay, hyperactivity was observed in all three outcomes in dark phases in larvae with exposure (1-120 h post fertilization, hpf) to 10 ppb As, 10 ppb Pb, or 10 ppb mix treatment. Time spent moving exhibited hyperactivity in dark phases for 100 ppb As and 100 ppb mix treatment groups only. A decreased brain length and ratio of brain length to total length in the 10 ppb mix group was measured with no alterations in other treatment groups or other endpoints (i.e., total larval length, head length, or head width). Alternatively, measurements of cerebral vasculature in the midbrain and cerebellum uncovered decreased total vascularization at 72 hpf in all treatment groups in the mesencephalon and in all treatment groups, except the 100 ppb Pb and 10 ppb As groups, in the cerebellum. In addition, decreased sprouting and branching occurred in the mesencephalon, while only decreased branching was measured in the cerebellum. The 10 ppb Pb group showed several cerebral vasculature modifications that were aligned with a specific gene expression alteration pattern different from other treatment groups. Additionally, the 100 ppb As group drove gene alterations, along with several other endpoints, for changes observed in the 100 ppb mix treatment group. Perturbations assessed in this study displayed non-linear concentration-responses, which are important to consider in environmental health outcomes for As and Pb neurotoxicity.

Nanovesicular Mediation of the Gut-Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome.

Dysbiosis, influenced by poor diet or stress, is associated with various systemic diseases. Probiotic supplements are recognized for stabilizing gut microbiota and alleviating gastrointestinal issues, like irritable bowel syndrome (IBS). This study focused on the tryptophan pathways, which are important for the regulation of serotonin levels, and on host physiology and behavior regulation. Nanovesicles were isolated from the plasma of subjects with chronic diarrhea, both before and after 60 days of consuming a probiotic mix (Acronelle®, Bromatech S.r.l., Milan, Italy). These nanovesicles were assessed for the presence of Tryptophan 2,3-dioxygenase 2 (TDO 2). Furthermore, the probiotics mix, in combination with H2O2, was used to treat HT29 cells to explore its cytoprotective and anti-stress effect. In vivo, levels of TDO 2 in nanovesicles were enhanced in the blood after probiotic treatment, suggesting a role in the gut-brain axis. In the in vitro model, a typical H2O2-induced stress effect occurred, which the probiotics mix was able to recover, showing a cytoprotective effect. The probiotics mix treatment significantly reduced the heat shock protein 60 kDa levels and was able to preserve intestinal integrity and barrier function by restoring the expression and redistribution of tight junction proteins. Moreover, the probiotics mix increased the expression of TDO 2 and serotonin receptors. This study provides evidence for the gut-brain axis mediation by nanovesicles, influencing central nervous system function.

Inorganic Nutrient Composition and Frequency of Ratoon on Kangkong (Ipomoea reptans Poir) in Pot Trial Using Ultisol Media for Urban Farming

Kangkong (Ipomoea reptans) is a type of vegetable that is quite easy to cultivate. Cultivation of kangkong with a ratoon system is more efficient in cultivation time and input, and harvesting can be done more than once. Yield of ratoon cultivation system is influenced by ratoon frequency and nutrition. This study aims to determine the effect of inorganic nutrient composition and ratoon frequency. The research was conducted from January to March 2022 at the University of Bangka Belitung. The research method used factorial Completely Randomized Design (CRD). The first factor was the composition of inorganic nutrients, consisting of N1 (AB mix), N2 (NPK + Gandasil D), and N3 (NPK + Growmore). The second factor is the frequency of ratoon, consisting of R0 (no ratoon), R1 (ratoon frequency of 1), R2 (ratoon frequency of 2), and R3 (ratoon frequency of 3). The results revealed that treatment combinations had no significant effect on all observed variables. Composition of inorganic nutrients have a significant effect on root variables with the AB mix treatment as the best. Ratoon frequency treatment had a significant effect on almost all variables (except total number of leaves) with the best treatment being without ratoons (R0). The average total number of leaves of kangkong plants showed the highest yield in the N3R3 treatment, reaching 14.52 strands and the lowest yield in the N1R1 treatment with 12.81 strands. Keywords: Kangkong, Inorganic nutrient, Ratoon frequency, Plant growth, Yield,

Nutrient Cycling with Duckweed for the Fertilization of Root, Fruit, Leaf, and Grain Crops: Impacts on Plant–Soil–Leachate Systems

The increasing energy required to synthesize inorganic fertilizers warrants more sustainable soil amendments that produce comparable crop yields with less environmental damage. Duckweed, a prolific aquatic plant, can not only sequester carbon dioxide through photosynthesis, but also hyperaccumulate nutrients from its environment and upcycle them into valuable bioproducts. In this study, dried duckweed, grown on treated wastewater treatment plant effluent, was utilized as a fertilizer for a variety of crops (beet, tomato, kale, and sorghum). Comparative experiments examined the effect of duckweed, inorganic fertilizer, and a 40–60 mix of both on crop yield and nutrient fate in the plants, soil, and leachate. Comparable yields of beet, tomato, and sorghum were generated with duckweed and inorganic fertilizer. Duckweed significantly enhanced phosphorus (P) uptake in sorghum, exhibiting a P use efficiency level of 18.48%, while the mix treatment resulted in the highest P use efficiencies in beet and tomato. Duckweed-amended beet and kale systems also increased residual soil N (0.9% and 11.1%, respectively) and carbon (4.5% and 16.6%, respectively). Linear regression models developed using the data collected from all crops confirmed that duckweed can be used as a substitute for inorganic fertilizer without negative effects to food yield or nutritional quality.

Arbuscular Mycorrhizal Fungi, Especially Rhizophagus intraradices as a Biostimulant, Improve Plant Growth and Root Columbin Levels in Tinospora sagittata

Tinospora sagittata is a columbin-rich medicinal plant, but its columbin levels are reduced under artificial cultivation conditions. The objective of this study was to analyze the effects of inoculations with Diversispora versiformis (Dv), Funneliformis mosseae (Fm), Rhizophagus intraradices (Ri), and mixed inoculation (Dv + Fm + Ri) (Mix) on growth performance, root morphology, leaf photosynthetic physiology, and root columbin levels in T. sagittata. These arbuscular mycorrhizal fungi (AMF) were able to colonize the roots, as evidenced by a root mycorrhizal colonization rate ranging from 17% to 48% and soil hyphal lengths ranging from 17.51 cm/g to 32.02 cm/g, with the Mix treatment being the greatest. AMF inoculations improved plant height (16–151%), leaf number (119–283%), shoot (37–211%), and root biomass (22–318%) to varying extents, with Ri and Mix treatments being the most prominent. AMF-treated plants presented relatively greater root total length, projected area, surface area, volume, and average diameter, especially those treated with Ri and Mix. AMF inoculations also significantly improved the leaf nitrogen balance index, transpiration rate, and stomatal conductance, while the photosynthesis rate and chlorophyll index varied by AMF species, along with a decrease in intercellular CO2 levels. Root columbin levels ranged from 0.524 mg/g to 5.389 mg/g, and AMF inoculation significantly increased root columbin levels by 228–928%, with Ri being the most significant. Root columbin levels were significantly positively correlated with soil hyphal length, root total length, root projected area, root surface area, root volume, and root average diameter, but not root AMF colonization rate. This study demonstrates for the first time that AMF, especially Ri, can be employed as a biostimulant to promote growth as well as root columbin levels in T. sagittata, where AMF-triggered improvement in root morphology is an important reason for promoting root columbin levels.

Bacillus subtilis and Bacillus amyloliquefaciens Mix Suppresses Rhizoctonia Disease and Improves Rhizosphere Microbiome, Growth and Yield of Potato (Solanum tuberosum L.).

Black scurf and stem canker caused by Rhizoctonia solani is a significant disease problem of potatoes. Currently, chemical methods are the primary means of controlling this pathogen. This study sought to explore an alternative approach by harnessing the biocontrol potential of a bacterial mix of Bacillus subtilis and Bacillus amyloliquefaciens against black scurf, and to determine their effect on rhizosphere microorganisms of soil microbiota. This study showed that these bacteria demonstrate antagonistic activity against Rhizoctonia solani. Reduced damage to potato plants during the growing season in Siberia was observed. The index of disease development decreased from 40.9% to 12.0%. The treatment of tubers with this mix of bacteria also led to a change in the composition of the rhizosphere microbiota (according to CFU, 16S and ITS sequencing). This effect was accompanied by a positive change in plant physiological parameters (spectrophotometric analysis). The concentration of chlorophyll in potatoes with the bacterial mix treatment increased by 1.3 fold (p ≤ 0.001), and of carotenoids by 1.2 fold (p ≤ 0.01) compared with the control. After bacterial mix treatment, the length of the aerial parts of plants was 1.3 fold higher (p ≤ 0.001), and the number of stems 1.4 fold higher (p ≤ 0.05). The yield of potatoes was increased by 8.2 t/ha, while the large tuber fraction was increased by 16% (p ≤ 0.05). The bacteria mix of Bacillus subtilis and Bacillus amyloliquefaciens suppressed the plant pathogenic fungus Rhizoctonia solani, and simultaneously enhanced the physiological parameters of potato plants. This treatment can be used to enhance the yield/quality of potato tubers under field conditions.

Seed sourcing for climate-resilient grasslands: The role of seed source diversity during early restoration establishment.

Restoration advocates for the use of local seed in restoration, but theory suggests that diverse seed sources may enhance genetic diversity and longer term evolutionary potential within restored communities. However, few empirical studies have evaluated whether species and genetic diversity within species impacts plant community composition following restoration. The goal of this research is to compare the effects of single and multi-sourced seed mix treatments on plant community diversity following restoration. Species establishment, abundance, and diversity were compared following two restoration seed mix treatments created to include 14 species commonly used in grassland restoration. We compared the application of seed mixes designed using a single population per species with those containing five populations per species across sites in Minnesota and South Dakota, United States. Early plant establishment and richness mostly reflected non-seeded species across both sites, although seeded species established at a slightly higher rate in year two following restoration. At the South Dakota site, community composition largely reflected changes associated with establishment across the growing season as opposed to seed mix treatment. This contrasted with the Minnesota site, where community composition appeared to be strongly influenced by seed mix treatment. While there is some evidence seed mix treatment may be influencing the emergent community across sites, spatial heterogeneity across the Minnesota restoration site likely influenced diversity in early emergence over that of seed mix treatment. Indeed, varying land-use history across both sites likely contributed to differences in species composition observed at this early stage of the restoration. This suggests that seed mix treatment may have limited impact on early post-restoration emergence diversity relative to the importance of land-use history. However, future monitoring will be needed to evaluate whether the impact of seed mix treatment on community composition changes over time.

Effects of dietary ginger (Zingiber officinale) extract and curcumin on growth, hematology, immunity, and antioxidant status in rainbow trout (Oncorhynchus mykiss)

The use of dietary ginger (Zingiber officinale) and curcumin as immunostimulants is a promising method in fish culture. This study evaluated the impacts of dietary ginger extract and curcumin on growth, hematology, immune and antioxidant status, and expression of some growth and immune related genes in rainbow trout (Oncorhynchus mykiss) fingerlings. Fish (7.5 ± 0.1 g) were randomly distributed into 12 fiberglass tanks (30 fish per tank), divided into four treatments in triplicate and received no supplementation (control) and diets supplemented with 0.5% ginger extract (ginger treatment), 0.5% curcumin (curcumin treatment), and 0.5% ginger extract plus 0.5% curcumin (mix treatment). The growth of fish and muscle crude protein content were highest in the mix treatment. Higher protein efficiency ratio, hemoglobin, and plasma ALP activity were observed in fish fed supplemented diets than the control. Red blood cells (RBC) counts and hematocrit value were higher in the ginger treatment, then curcumin and mix treatments and lower in the control. White blood cells (WBC) counts and plasma lysozyme, ACH50, catalase, and SOD activities were highest in fish fed the mix diet. Moreover, plasma C4 value and GPx activity were significantly higher and triglyceride value was lower in the curcumin and mix treatments compared to the control and ginger treatments. The highest plasma C3 and total immunoglobulin (Ig) values were found in fish fed curcumin and mix diets, but those were not significantly different from the ginger treatment. Growth hormone (GH) and lysozyme relative expression levels were highest in fish fed the mix diet and lowest in the control. Thus, the combination of 0.5% ginger extract and 0.5% curcumin (mix diet) to improve growth, body composition, hematology, immune responses, and antioxidant status in rainbow trout can be recommended.

Hidroponik Organik Berbasis Pupuk Organik Cair

Organic hydroponics based on liquid organic fertilizer. Research on the cultivation of Kailan (Brassica oleracea var. achepala) plants using an organic hydroponic system based on organic fertilizers (POC), was conducted at the experimental garden of Pasraman Unud Jimbaran from March to June 2020. The liquid organic fertilizer used consisted of two types of MOL, namely bamboo shoots MOL and banana weevil MOL. The purpose of this research is to find an organic medium (nutrient solution) that is cheap, easy and environmentally friendly as an alternative substitute. AB mix (inorganic nutrients) which has been used in hydroponic systems. The experimental design used in this study was a Randomized Block Design. Using liquid organic fertilizer from MOL Bamboo Shoots (R) and MOL Banana Hump (B). The treatments consisted of: K (without MOL); K+ (without MOL +AB mix) ; R1& B1 ( 50 ml/L ); R2 & B2 (100 ml/L) ; R3 & B3 (150 ml/L); and R4 & B4 (200 ml/L), there were 10 treatments with 3 replications, so there were 30 experimental units. Initial analysis of the media was carried out on the levels of N, P, K, pH and EC. Parameters measured were number of leaves, plant height, wet weight, plant dry weight. The results showed that hydroponics by only relying on liquid organic fertilizer as a source of nutrition, was not able to support normal plant growth. Wet weight, dry weight and plant height of Kailan in the MOL treatment of banana weevil and MOL of bamboo shoots showed much smaller results, namely 1/10 compared to the AB mix (K+) treatment.

Beneficial microorganisms: a sustainable horticultural solution to improve the quality of saffron in hydroponics

Saffron (Crocus sativus L.) is the most expensive spice in the world. Its organoleptic properties are mainly dictated by the apocarotenoids crocins (dyeing capacity), picrocrocin (pleasant taste), and safranal (scent). Cultivation of saffron in controlled conditions with beneficial microorganisms may increase its profitability. In this study saffron was grown in hydroponics under a greenhouse with the PGPR Bacillus megaterium CB97032 and Paenibacillus durus CB1806 (Pgpr treatment), and the AMF Rhizophagus intraradices (Myc treatment), alone or mixed (Mix treatment). The influence exerted during all crop phases on flowering trend, flower and spice yield, secondary metabolites, ecophysiological traits, and corm production was investigated, with the hypothesis that microbial synergy would have more positively affected these parameters. All the bioinoculants did not positively influence flower and spice yield, but enhanced the content of safranal (up to +96% in all the treated plants). When mixed, the PGPR and AMF improved the total phenolic content (+19%) of the saffron spice. Even if no differences emerged from the ecophysiological analysis, the corm yield was improved for the inoculated plants. The single-type bioinoculants allowed to obtain a higher number of replacement corms (up to +13% for the Myc plants) without reducing their weight, but lowering their size. When mixed, the size of the corms was restored. Together, the PGPR and AMF also increased the corm weight (+24%) of the largest corm fraction (> 1.5 cm). Thus, the bioinoculants may have stimulated the secondary metabolism of the plants by improving quality traits, rather than having acted as biofertilizers by increasing yield, at least during flowering. Overall, AMF and PGPR were proved to be a sustainable horticultural solution in hydroponics to improve the quality of saffron, especially when applied in mixed formulations.

Diet Preference, Feed Efficiency and Expression of the Sodium-Dependent Glucose Transporter Isoform 1 and Sweet Taste Receptors in the Jejunum of Lambs Supplemented with Different Flavours.

This study investigated the effect of dietary flavour supplements on the preference, feed efficiency and expression of the sweet taste receptor family 1 members 2 and 3 (T1R2 + T1R3), and sodium-glucose linked transporter 1 (SGLT1) genes in the lambs' small intestines. Eight, five-month-old, Israeli crossbred Assaf lambs were offered 16 different non-nutritive commercial flavours in rolled barley and ground corn. Capsicum and sucram were the most preferred non-aroma flavours (p = 0.020), while milky (p < 0.001) was the most preferred powder-aroma flavour. For the metabolic and relative gene expression study, eight lambs were randomly assigned to either sucram, capsicum, a mix containing sucram and capsicum at 1:1 ratio or no flavour for control in a 4 × 2 cross-over design. The total collection of urine (females only), faeces and refusals was carried out, and T1R2, T1R3 and SGLT1 relative gene expression evaluated from the proximal jejunum biopsies. Flavour had no significant effect on the feed intake (p = 0.934), but capsicum increased the average daily weight gain per metabolic body weight (p = 0.049). The T1R3 gene was expressed highest in the mix treatment (1.7; p = 0.005). Collectively, our findings indicate that flavours can be used to motivate feed acceptance and improve the weight gain in lambs.

Pigeon Pea Intercropped with Tropical Pasture as a Mitigation Strategy for Enteric Methane Emissions of Nellore Steers.

In this study, we evaluate the effects of intercropping pigeon pea (Cajanus cajan (L.) Millsp.) with tropical pastures for feeding Nellore cattle and compared animal performance and enteric CH4 emissions with other pasture-based systems during the dry and rainy seasons of 2021. Thirty-six Nellore steers (with a body weight of 221 ± 7 kg and an age of 15-16 months) were randomly distributed in three treatments with three replicates (in paddocks of 1.5 hectares each): (1) a degraded pasture of Urochloa spp. (DEG); (2) a recovered and fertilized pasture of Urochloa spp. (REC); and (3) pigeon pea intercropped with Urochloa spp. (MIX). Enteric CH4 emissions were estimated using the sulfur hexafluoride (SF6) tracer gas technique, and dry matter intake (DMI) was determined using internal (iNDF) and external (TiO2) markers. Forages were collected by hand plucking after observations of ingestive behavior, and feces was collected after voluntary defecation. The proportion of grass and legume intake was estimated by C stable isotopes, and the forage nutritional quality was determined, while animal performance was monitored monthly, and the stocking rate was adjusted by the "put and take" technique. The results indicated that intercropping pigeon pea with tropical grasses is an interesting strategy for sustainable livestock production based on pastures. The MIX treatment was able to meet the nutritional requirements of the animals, which presented higher performance. In addition, there was a reduction in CH4 emissions up to 70% when expressed per average daily weight gain in comparison to the DEG treatment.

Extracts of Apricot (Prunus armeniaca) and Peach (Prunus pérsica) Kernels as Feed Additives: Nutrient Digestibility, Growth Performance, and Immunological Status of Growing Rabbits

This study assessed the effects of the kernel extracts of apricot (AKE; Prunus armeniaca) and peach (PKE; Prunus pérsica), and their mixture (Mix) on growth efficiency, feed utilization, cecum activity, and health status, of growing rabbits. Weaned male New Zealand White rabbits at six weeks old [n = 84, 736 ± 24 SE g body weight (BW)] were randomly allotted to four dietary groups. The first group received no feed additives (control), the second and third groups received 0.3 mL/kg BW of AKE and PKE, respectively, and the fourth group received a mixture of AKE and PKE (1:1) at 0.3 mL/kg BW (Mix). Results indicated that 2(3h)-Furanone, 5-Heptyldihydro was found in abundance in both extracts, while 1,1-Dimethyl-2 Phenylethy L Butyrate and 1,3-Dioxolane, and 4-Methyl-2-Phenyl- were the most components detected in AKE and Cyclohexanol and 10-Methylundecan-4-olide were found in abundance in PKE. All the experimental extracts enhanced (p < 0.05) the growth performance, cecal fermentation parameters, and cecal L. acidiophilus and L. cellobiosus count, while PKE and the mixture treatments presented the highest (p = 0.001) total weight gain and average weight gain without affecting the feed intake. Rabbits that received the mix treatment had the highest (p < 0.05) nutrient digestibility and nitrogen retained, and the lowest (p = 0.001) cecal ammonia concentration. All the experimental extracts enhanced (p < 0.05) the blood antioxidant indicators (including total antioxidant capacity, catalase, and superoxide dismutase concentrations), and immune response of growing rabbits. In general, fruit kernel extracts are rich sources of bioactive substances that can be used as promising feed additives to promote the growth and health status of weaned rabbits.

Neraca Kehidupan Spodoptera pectinicornis (Hampson) dengan Pakan Gulma Kayu Apu Pistia stratiotes Linn yang diberi Pupuk NPK dan AB Mix

This study aims to examine the life balance of the biological control agent Spodoptera pectinicornis fed Pistia stratiotes with NPK and AB mix fertilization.. The research was carried out from March to May 2021 at the Laboratory Pengendalian Hayati and Greenhouse Entomology Faculty of Agriculture University Lambung Mangkurat Banjarbaru. The data in this study were analyzed descriptively by calculating the population. S. pectinicornis in one life cycle. There are 3 treatments and 3 replications on the 100 egg s. pectinicornis, The highest gross reproductive rate (GRR) was in the AB mix treatment of 1912 individuals/generation. The highest net reproduction rate (Ro) was found in the AB mix treatment of 129.520. The average value of the shortest generation period (T) in the NPK fertilizer treatment was only 26.334 days. The highest intrinsic growth rate (r) was found in the AB mix fertilizer treatment of 0.164 individuals/parent/day. The calculation of the limited growth rate (λ) showed that the AB mix treatment had the highest population increase of 1,174 individuals/parent/day. Apu wood treated with NPK and AB mix fertilizers can increase the rate of population development in the life table of the insect S. pectinicornis.

A novel photoanaerobic process as a feasible alternative to the traditional aerobic treatment of refinery wastewater

Refinery wastewater (RWW) treatment is outdated since new wastewater management and reuse challenges require more environmental-friendly and cheap alternatives. Conventional biological treatments focused on activated sludge are highly energy-intensive and resource-dissipating processes. However, anaerobic treatments are an excellent alternative to reduce costs derived from aeration and carbon footprint. This work proposes a novel strategy for the treatment of RWW involving a photoanaerobic membrane bioreactor (PAnMBR) with a mixed culture of purple phototrophic bacteria (PPB). PPB upcycles the organic matter, nitrogen, and phosphorus in an assimilative way, leading to a much higher biomass yield and nutrient removal than aerobic cultures. The enriched PPB culture was generated from the RWW as the sole substrate without specific PPB inoculation. The RWW (exempted from sufficient nutrients) was successfully treated with additional ammonium and phosphates provided by domestic wastewater (DWW). Preliminary batch tests determined the best DWW/RWW volumetric mixing ratio at 25:75. The PAnMBR was operated for 144 days under different specific loading rates (SLR) by modifying hydraulic and solid retention times. The maximum specific loading rate (SLR) for the efficient RWW/DWW mix treatment was 0.3 mgCODinlet/mgCODbiomass·d. The COD consumption was mainly mediated by Rhodopseudomonas sp. and Rhodobacter sp. PPB genera. The PPB-based photo-anaerobic membrane reactor was able to comply with regulated parameters for wastewater discharge for the more restrictive use of reclaimed water according to the European legislation in force.

Efektivitas Rendaman Bawang Merah (Allium cepa) dan Nutrisi Terhadap Hasil Hidroponik Pakcoy (Brassica rapa)

Hydroponic cultivation systems are increasingly popular among urban communities with not large land. This cultivation can provide benefits and effectiveness for those who carry it out. The wick system is one of the simple hydroponics methods that is easy to do, because it is a passive hydroponic system. This study aims to find out how the effect of shallot solution to the growth and production of bok coy plants on hydroponic cultivation of the wick system. Hydroponics of the wick system is a hydroponic method of the axis system, which is the simplest because it uses the principle of water capillarity. Distribution of nutrient solution towards root using axis intermediaries. This study used a Randomized Group Design (RDG) with 12 experimental units and the Least Significance Different (LSD) with an error rate of 5%. The concentration of shallot solution added is P0 (0%), P1(20%), and P2 (30%). The results showed that the treatment of AB mix nutrition as a hydroponic medium has a tendency to have a better effect on the growth parameters and yield of Pakcoy plants. AB mix treatment had a significant effect on production parameters, namely total wet weight, upper wet weight, root wet weight, and dry weight. Provision of shallot soak at concentrations of 20% and 30% added to hydroponic media with AB mix nutrition has not been able to improve the growth parameters and yield of Pakcoy.

Perbandingan Pertumbuhan Pagoda antara Larutan Nutrisi AB Mix dan Pupuk Organik Cair pada Sistem Hidroponik NFT

Pagoda is a leaf vegetable plant that has a unique shape when compared to other types of mustard greens because the shape of this pagoda mustard is similar to a blooming flower, oval leaf shape with a very striking dark green colour, short-lived and has many nutrients and benefits. Vegetable cultivation technique using a hydroponic system that is often used is NFT (Nutrient Film Technology) because the design is quite simple to use. An important factor that must be considered in a hydroponic system to obtain optimal plant growth results is the need for nutrients. AB mix nutrition is a chemical fertilizer which is one of the standard nutrients used in hydroponic systems. The use of Liquid Organic Fertilizer (LOF) is expected to be an alternative hydroponic nutrient solution so that the vegetables produced are healthier, or what we are familiar with organic vegetables. The aim of the study was to compare the growth of pagoda plants against two types of nutrient solutions (AB mix and LOF) in the NFT hydroponic system. The research was carried out from March to April 2019 at Greenhouse, East Jakarta. The experimental design used was a completely randomized design. The treatment was the type of nutrient solution (AB mix and LOF) and was repeated 3 times. Observational data were analysed using t-test at 5% significance level. The results showed that pagoda growth in AB mix treatment was better than LOF so that LOF had not been able to become an alternative to AB mix for hydroponic nutrient solution for pagoda plants.

Lead and copper led to the dysregulation of bile acid homeostasis by impairing intestinal absorption in Bufo gargarizans larvae: An integrated metabolomics and transcriptomics approach

Bile acids, as metabolic regulators and signaling molecules, play key roles in the regulation of host metabolism and immune responses. Heavy metals such as lead (Pb) and copper (Cu) are widespread environmental pollutants that threaten public health. However, the effects of heavy metals on bile acid metabolism and the underlying molecular mechanisms remain unclear, particularly for ecologically important amphibian species. In the present research, the effects of exposure to environmentally-relevant concentrations of Pb (250 μg/L), Cu (50 μg/L), and a mixture of both (Mix) on bile acid metabolism and the underlying molecular mechanisms in the intestines of Bufo gargarizans larvae were comprehensively investigated using histopathology, metabolomics and transcriptomics analysis. Our results suggested that Pb and/or Cu caused histopathological damage to the intestine and liver, such as decreased intestinal epithelial cell height and dilated hepatic sinusoid. The total bile acid level was decreased in the Pb and Mix exposure groups but elevated in the Cu treatment. A significant decrease in the ratio of conjugated to unconjugated bile acids was present in all treatment groups. Also, the level of GCA was increased while TCA and TCDCA were decreased in all exposure groups. In addition, exposure to Pb and Cu altered the expression levels of genes related to intestinal absorption. For example, mrp2, mrp3 and aqp4 had higher expression in the Pb and Mix treatment groups, and aqp1 and mrp4 were increased in the Cu treatment group. Overall, we speculated that the dysregulation of bile acid homeostasis induced by Pb and Cu exposure may be due to impaired intestinal absorption. These findings raise further concerns about the hazards of Pb and/or Cu in influencing bile acid metabolism that might lead to the development of metabolic diseases and inflammatory disorders.