Lyon CA, Davis JL, Fincel MJ, Chipps SR. 2022. Effects of capture depth on walleye hooking mortality during ice fishing. Lake Reserv Manage. XX:XXX–XXX. Length-based regulations are a common tool used to limit fishing mortality by controlling the size of fish harvested. While such regulations are helpful in managing fish populations, mortality associated with catch-and-release fishing may negatively impact a fishery. We evaluated factors affecting hooking mortality of walleye (Sander vitreus) in 2 mainstem Missouri River reservoirs in South Dakota. Winter walleye hooking mortality was evaluated during the ice fishing season in February and March 2020. After capture, walleye (n = 55) were placed into holding pens for 12 to 72 h to monitor postrelease mortality. Hooking mortality was found to be 20% following angling. Capture depth, landing time, and time in pen were the most influential variables on probability of hooking mortality (pm ). We observed a sharp increase in pm for walleye captured at depths from 10 to 12 m, where the probability of mortality for fish increased appreciably from 5 to 37%, respectively. Our findings indicate that hooking mortality during the ice fishing season can be substantial in lakes where walleye angling occurs at depths greater than 10 m.