Mint Essential Oil Research Articles

Study on the chemical constituents and anti-free radical activity of mint essential oils in Binh Thuan Province, Vietnam

Mints (Mentha spp.) are famous aromatic and medicinal herbs that are used in traditional and folk medicines around the world for their antimicrobial and antioxidant properties. Mints contain volatile components such as flavonoids, organic acids, and quinones for the digestive system, central nervous system and respiratory system. Therefore, this study aimed to primarily find out the constituents and antioxidant activity of mint essential oil, which are very essential. The mint was harvested from the Ham Thuan Nam district of Binh Thuan province, Vietnam, in 2023. The essential oil of the mint was collected using steam distillation and dried with Na2SO4 for the final product. Chemical analysis of the components of the mint essential oil by GC-MS led to the identification of 17 chemical components, representing 98.72% of the hydro-distilled essential oil. Of these, five were hydrocarbons, such as monoterpenes (32.82%), and the rest were oxygenated hydrocarbons, such as alcohols (53.42%), aldehydes (4.67%), and ketones (7.81%). The 1,1-diphenyl-2-picrylhydrazol free radical scavenging activity of the mint essential oil was 39.36±1.23%, and this value was lower than that of 0.5 mM ascorbic acid (42.84 ±1.18%). Thus, the results of this study have high scientific and practical significance, when adding peppermint essential oil to food, it not only creates a fragrant smell for food, but also has antioxidant and antibacterial effects for food.

Mint (Mentha spp.) essential oil extraction: from conventional to emerging technologies

Mint (Mentha spp.) essential oil extraction: from conventional to emerging technologies

Performance evaluation of tea tree oil, eucalyptus oil & mint essential oil on cotton (knit) fabric as mosquito repellent finish

Performance evaluation of tea tree oil, eucalyptus oil & mint essential oil on cotton (knit) fabric as mosquito repellent finish

Antimicrobial Activity of Essential Oils Against Microbes Associated with Street Food

This review aims to evaluate the effectiveness of five essential oils (EOs), namely clove, lemongrass, mint, oregano, and black cardamom, at different concentrations, in fighting microorganisms that were isolated from selected food samples obtained from local street food vendors. The microorganisms isolated from the food samples were Enterobacter sp., Pseudomonas sp., Proteus sp., Acinetobacter sp., Moraxella sp., Escherichia sp. (gram-negative bacteria), and Staphylococcus sp., Bacillus sp., Streptococcus sp., Lactobacillus sp., Micrococcus sp. (gram-positive bacteria). The antimicrobial effects of these oils were tested, and the results were analyzed using one-way analysis of variance (ANOVA) with a significance level of p<0.001. The tests were carried out using different concentrations of 20%, 40%, 60%, 80%, and 100% (v/v) against isolates obtained from raw and street food. It was found that clove oil had the highest effect, followed by mint oil, black cardamom oil, and oregano oil. However, lemongrass oil showed the least antimicrobial effect. The results indicate that clove, mint, and black cardamom EOs are highly effective in controlling pathogenic bacteria. These oils can be used to prevent bacterial growth, extend shelf life, and increase the safety of street food.

In vitro inhibition of Candida spp., strains by wild mountain mint essential oil: A traditional medicinal plant in the high Andean regions of Peru

In vitro inhibition of Candida spp., strains by wild mountain mint essential oil: A traditional medicinal plant in the high Andean regions of Peru

Effects of Mint Oils on the Human Oral Microbiome: A Pilot Study.

The oral microbiome is a diverse and complex ecosystem essential for maintaining oral and systemic health. Our study is the first to define the oral microbial community in Egyptian young adults and investigate the effects of natural antimicrobials on the oral microbiome. SuperMint (SM) is a proprietary blend of peppermint, Japanese mint, bergamot mint, and spearmint essential oils encapsulated in a tiny soft beadlet. This work aimed to evaluate the effects of SM beadlets on the oral microbiome. This study recruited twenty healthy participants. A baseline investigation of the oral microbiome of the selected participants was performed by collecting saliva and swab samples before treatment. Treatment included chewing four SM beadlets twice a day for 7 days, and then, post-administration saliva and swab samples were collected at the end of treatment. The oral microbiome samples were analyzed by the high-throughput amplicon sequencing of 16S rRNA gene fragments, and the community composition was determined. The results showed that the abundance of some microbial genera and families decreased after using SM, including Prevotella, Streptococcus, Neisseria, and Haemophilus. However, some genera showed inconsistent patterns. We also found that the subject's gender and SM usage were significantly associated with diverse microbial composition. The results suggest that SM treatment decreased the abundance of several bacteria associated with halitosis and periodontal diseases, such as Actinomyces and Streptococcus. Furthermore, Corynebacterium species increased and Streptococcus decreased after SM usage. More research is needed to fully understand the antimicrobial effects of mint oils and their potential applications in maintaining good oral health.

Laboratory Efficacy of Essential Oils Against Pectobacterium carotovorum Subsp. carotovorum and Pectobacterium atrosepticum Causing Soft Rot of Potato Tubers

AbstractThe antibacterial activity of essential oils (EOs) from Carum carvi, Cinnamomum zeylanicum, Cuminum cyminum, Eugenia caryophyllus, Foeniculum vulgare, Melaleuca alternifolia, Mentha × piperita, Origanum vulgare, Rosmarinus officinalis and Thymus vulgaris was tested against Pectobacterium carotovorum subsp. carotovorum (Pcc) and Pectobacterium atrosepticum (Pa), which cause soft rot of potato tubers. In disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration (MBC) tests, cinnamon EO was found to be most effective against both bacteria. The inhibition zones ranged from 20.46 to 29.58 mm for a concentration of 100 μL/mL. The minimum inhibitory concentration was 0.5 μL/mL, and MBC was between 0.5 and 5 μL/mL. The higher sensitivity of bacteria was manifested in clove (Pcc and Pa), mint (Pcc), oregano (Pa) and thyme (Pa) EOs. Rosemary EO was the least effective. The results of the in vivo test were not entirely consistent with those of the in vitro tests. The most significant antibacterial effect was achieved with mint EO. The treatment of potato tuber discs with mint EO at a concentration of 3 μL/mL for Pcc and 3–10 μL/mL for Pa was 100% effective. The efficacy of the essential oils of caraway (5–10 μL/mL), thyme (10 μL/mL) and oregano (5 μL/mL) also ranged from 95.7 to 99.7%. Based on the results of the in vivo test, it may be recommended that mint EO and potentially caraway, oregano and thyme EOs be further tested for pickling potato tubers against bacteria of the genus Pectobacterium.

Comprehensive Modulation of Secondary Metabolites in Terpenoid-Accumulating Mentha spicata L. via UV Radiation.

In plants, secondary metabolites change in response to environmental conditions. These changes co-regulate resilience to stressful environmental conditions, plant growth and development, and interactions between plants and the wider ecosystem, while also affecting soil carbon storage and atmospheric and climatic conditions. The objective of this study was to determine the association between UV exposure and the contents of key metabolites, including amino acids, phenolics, flavonoids, terpenoids, carotenoids, tocopherols, and phytosterols. Mentha spicata plantlets were grown in tissue culture boxes for 30 days and then exposed to a low dose of broadband UV-B (291-315 nm; 2.8 kJm-2 biologically effective UV) enriched light for eight days. Metabolite contents were quantified either immediately after the final UV exposure, or after seven days of recovery under photosynthetically active radiation. It was found that UV promoted the production of flavonoids (1.8-fold) ahead of phenolic acids (unchanged). Furthermore, the majority of monoterpenes and sesquiterpenes, constituents of valuable mint essential oil, were significantly increased through UV treatment (up to 90-fold for α-linalool). In contrast, the contents of carotenoids and tocopherols did not increase following UV exposure. A comparison between plants sampled immediately after UV exposure and after seven days of recovery showed that there was an overall increase in the content of carotenoids, mono- and sesquiterpenes, phenolics, and amino acids following recovery, while the contents of sterols and tocopherols decreased. These UV-induced changes in metabolite profile may have important consequences for agriculture, ecology, and even the global climate, and they also provide an exciting opportunity to enhance crop value, facilitating the development of improved products with higher levels of essential oils and added benefits of enhanced flavour, colour, and bioactive content.

A cost-benefit analysis of essential oil extraction technology of aromatic plants in Pothwar Punjab, Pakistan

Enhancing rural livelihoods in low to medium-income countries through innovative agricultural technologies is a critical challenge for decision-makers. Cost-effective interventions like essential oil extraction (EOE) technology for aromatic plants need rural adoption. The study assesses two EOE practices' economic benefits in Punjab, Pakistan, using three models: individual benefit-cost (BC), diffusion, and a Pothwar-specific BC model. Thus, analyzing results from the literature, expert insights, and indigenous knowledge can readily be employed in scenarios where data is limited in less developed economies, including Pakistan. The study findings revealed monetary costs and financial returns at both individual and zonal levels. The highest positive NPV value was recorded in the case of mint oil (267.86) followed by eucalyptus oil and wood (755.12) productivity for short-term and long-term benefits respectively. In addition, the maximum BC index (4.03) was recorded under eucalyptus oil and wood, followed by mint essential oil (3.82) production for 10 years. The model(s) further explored the key input parameters that positively impact the economic gains of the interventions and for ongoing future investments. Hence, the general application of the existing framework is also discussed, along with the limitations under different scenarios.

Effects of MhMYB1 and MhMYB2 transcription factors on the monoterpenoid biosynthesis pathway in l-menthol chemotype of Mentha haplocalyx Briq.

Transcription factors MhMYB1 and MhMYB2 correlate with monoterpenoid biosynthesis pathway in l-menthol chemotype of Mentha haplocalyx Briq, which could affect the contents of ( -)-menthol and ( -)-menthone. Mentha haplocalyx Briq., a plant with traditional medicinal and edible uses, is renowned for its rich essential oil content. The distinct functional activities and aromatic flavors of mint essential oils arise from various chemotypes. While the biosynthetic pathways of the main monoterpenes in mint are well understood, the regulatory mechanisms governing different chemotypes remain inadequately explored. In this investigation, we identified and cloned two transcription factor genes from the M. haplocalyx MYB family, namely MhMYB1 (PP236792) and MhMYB2 (PP236793), previously identified by our research group. Bioinformatics analysis revealed that MhMYB1 possesses two conserved MYB domains, while MhMYB2 contains a conserved SANT domain. Yeast one-hybrid (Y1H) analysis results demonstrated that both MhMYB1 and MhMYB2 interacted with the promoter regions of MhMD and MhPR, critical enzymes in the monoterpenoid biosynthesis pathway of M. haplocalyx. Subsequent virus-induced gene silencing (VIGS) of MhMYB1 and MhMYB2 led to a significant reduction (P < 0.01) in the relative expression levels of MhMD and MhPR genes in the VIGS groups of M. haplocalyx. In addition, there was a noteworthy decrease (P < 0.05) in the contents of ( -)-menthol and ( -)-menthone in the essential oil of M. haplocalyx. These findings suggest that MhMYB1 and MhMYB2 transcription factors play a positive regulatory role in ( -)-menthol biosynthesis, consequently influencing the essential oil composition in the l-menthol chemotype of M. haplocalyx. This study serves as a pivotal foundation for unraveling the regulatory mechanisms governing monoterpenoid biosynthesis in different chemotypes of M. haplocalyx.

On-farm energy input-output and economic analysis of important essential oil-bearing Mint species in subtropical India

Mint species are grown worldwide and known for their medicinal and aromatic properties however, studies on its energy assessment are very scarce. This study quantifies the energy input–output relationship of herbs and essential oil production of different mint species (M. arvensis, M. piperita, M. spicata, M. cardiaca and M. citrata) which were commercially grown in subtropical India. This study proposes for life cycle assessment and their effects on environment. The energy inputs and outputs were significantly varied with mints species. Results showed that mints essential oil production used more energy than herbs, and M. citrata utilizes the highest energy in both cases. Fire wood requirements for essential oil extraction accounts maximum (59.16 %) energy input. Mint herbs and essential oil yields were recorded higher in M. citrata (23.14 t ha−1), and M. arvensis (143.2 kg ha−1), respectively. Energy yields were estimated highest in M. cardiaca (herbs), and M. arvensis (essential oils), and required net calorific values were tested by automated advanced oxygen bomb calorimeter. Overall, M. arvensis showed the best results in energy production (0.07 kg MJ−1), profitability (0.27), net return (17156.40 MJ ha−1), and energy use efficacy (1.27) for mint herbs production. However, for essential oil production M. cardiaca performed well. This study revealed that mint herbs production was more energy efficient than essential oils extraction although essential oil production is fiscally secure.

Mint species (Mentha longifolia and Mentha spicata L) growth, essential oil generation and chemical components are impacted by turmeric curcumin applications

Mint essential oil has several applications in pharmacy and food industries. As mint species multiplied, active chemicals evolved, resulting in changes in their biological effects. Mint growth in Egypt’s sandy soils is hampered by abiotic stress. Phenols like turmeric curcumin reduce abiotic stress which plant suffers. Finding active chemicals in various aromatic plant species to substitute synthetic ones is an enticing approach to developing a new pharmaceutical regimen; these plants need to be studied to locate active components. Therefore, this study aimed to reduce the potential negative effects of abiotic stress in sand soil on horsemint (Mentha longifolia) and spearmint (Mentha spicata L) by applying turmeric curcumin (as organic elicitor), to assess if growing them in the new reclamation zones is a viable option in order to obtain essential oil naturally. Both species received 0, 30, 60, 90, and 120 mg L−1 turmeric curcumin. Horsemint exposed to 30 mg L−1 turmeric curcumin produced the maximum values of plant length (93 cm), fresh herb (114.73 g plant−1) and dry herb (33.81 g plant−1); spearmint × 30 mg L−1 turmeric curcumin resulted in the greatest amounts of chlorophyll a, chlorophyll b and carotenoids with values of 5.38, 1.83 and 1.45 mg g−1, respectively. The maximum values of eucalyptol (65.44%), camphor (23.81%), carvone (65.95%) and limonene (15.9%) were recorded under 90 mg L−1 turmeric curcumin. Horsemint plants subjected to 60 mg L−1 turmeric curcumin gave the highest essential oil yield (1.52 g plant−1), sesquiterpenes (31.4%), soluble sugars (102.19 mg g−1), free amino acids (8.73 mg g−1), phenols (3.71 mg g−1), flavonoids (5.28 mg g−1), free radical's inhibition (64.35%), protein (17.09%) and nitrogen (1.27 g plant−1); spearmint × 60 mg L−1 turmeric curcumin resulted in the greatest values of monoterpenes (98.76%), nitrogen (2.74%), phosphorous (0.54% or 0.17 g plant−1) and potassium (0.56% or 0.18 g plant−1). Control × spearmint gave the maximum value of sodium (0.17% or 0.04 g plant−1). To mitigate the detrimental effects of stress-related factors on sandy soil, turmeric curcumin can be applied to mint species; additionally, it could broaden the sources of natural products. On the other hand, this work provides as a guide for choosing mint species for usage in industries connected to essential oils based on pertinent components.

The capacity of Lamiaceae essential oils, extracts and hydrolats against pathogenic biofilms

The study aimed to analyse the potential of Lamiaceae essential oils, extracts, and hydrolats against bacterial biofilms. Bacterial cells Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus were exposed to Thymus vulgaris L. (thyme), Salvia officinalis L. (sage), Mentha × piperita L. (mint) essential oils, extracts, and hydrolats. The result of the minimal inhibitory concentration assessment shows the highest antibacterial potential for essential oils, followed by extracts and hydrolats respectively. The anti-biofouling capacity revealed that thyme essential oil has the highest potential for biofilm prevention for all tested bacteria, reducing up to 91% of biofilm, followed by mint (88%) and sage (87%) essential oil. While the thyme extract (84%), sage extract (83%) and hydrolat (77%) we less effective. The chemical composition of thyme essential oil showed a high percentage of monoterpene hydrocarbons and oxygenated monoterpenes, among which p-cymene and thymol were the most predominant. The bacterial cell membrane integrity assessment shows a significant increase in dead cells by increasing the concentration of thyme essential oil. The findings of our research indicate that the choice of herbal preparation significantly affects the active components, thereby influencing both antibacterial and anti-biofouling capabilities. Lamiaceae essential oils show great potential for biofilm management and represent a good candidate for antibacterial application in pharmacy, medicine, and industry.

Abstract 1251 "Purification and Characterization of Mountain Mint (Pycnanthemum muticum) Essential Oil and its Potential Use in Medicine and Food"

Abstract 1251 "Purification and Characterization of Mountain Mint (Pycnanthemum muticum) Essential Oil and its Potential Use in Medicine and Food"

Drug therapy possibilities for various forms of chronic rhinitis accompanied by dry nose syndrome

Introduction. Dry nose syndrome is a polyetiological condition that is common among patients of all age groups from infancy to profoundly old age. The low effectiveness of treatment is associated with the progression of atrophic mucous membrane degeneration, which also affects the cartilaginous and bony parts of the nasal cavity. Significant impairment of the protective, respiratory and olfactory function leads to decreased quality of life of patients, delayed adaptation, and arrested psychosomatic and physical development of children. Herbal medications refer to the agents that have minimal toxicity and side effects and effectively facilitate the restoration of functional integrity of the mucous membrane.Aim. To discuss the results of studies on the effectiveness of natural medicines in the treatment of patients with dry nose syndrome.Materials and methods. We conducted a literature search and studied publications (articles and relevant abstracts) containing information on various diseases that are accompanied by dry nose syndrome, as well as therapeutic options for this syndrome in Russian and foreign databases. The material was selected according to the following keywords: dry nose syndrome, atrophic rhinitis, mint essential oil, pine essential oil, eucalyptus essential oil. The study was conducted using the search engines Scopus, PubMed, CyberLeninka, Elibrary.ru, Google Scholar.Results and discussion. The study results suggested the potential and benefits of the topical use of a combination medicine containing essential oils of eucalyptus, mint, and pine as a treatment and prophylactic medication in patients with dry nose syndrome.Conclusion. Dyshesol, a herbal medicine that is an oil solution, meets the requirements for complex pathogenetic therapy in patients with dry nose syndrome of various etiologies, and can be recommended as a preventive and therapeutic medication for all age groups, and children from the age of two.

Investigation and assessment of the quality of Japanese mint essential oil (Mentha arvensis) originated from various geographical regions in Vietnam

In this study, the effects of distillation methods including hydro-distillation (HD) and microwave hydro-distillation (MHD) on essential oil extractions of Mentha arvensis L. in Lam Dong (LD), Ho Chi Minh City (HCMC), and Ba Ria-Vung Tau (BR) were investigated for the yield and chemical composition of the essential oil. The results showed that the use of MHD (0,075 %) was more effective on oil extraction yield than traditional HD (0.053 %). Optimal Distillation parameters were found distillation times of 20 and 110 minutes and material/water ratio of 1:2,5 and 1:6 g/mL for MHD and HD, respectively. The MHD method was subjected to comparing qualitative chemical compositions from Japanese essential oils in BR, LD, and HCMC, Vietnam. The GC-MS analysis results showed that menthol was a major constituent in the essential oil and was responsible for the distinctive smell and flavor of the Japanese mint. Moreover, the menthol content in oil from LD ound was up to 70.6 % higher than that in HCMC (69.45 %) and BR (67.85 %). In conclusion, the mint essential oil is distilled from ingredients in different regions of Vietnam, such as LD, HCMC, BR-VT, all give essential oil a characteristic scent and a relatively high menthol content of about 60 % - 70 %.

Performance Evaluation Of Tea Tree Oil, Eucalyptus Oil &amp;amp; Mint Essential Oil On Cotton (Knit) Fabric As Mosquito Repellent Finish

Performance Evaluation Of Tea Tree Oil, Eucalyptus Oil &amp;amp; Mint Essential Oil On Cotton (Knit) Fabric As Mosquito Repellent Finish

IN VITRO ANTIFUNGAL EFFECT OF TWELVE ESSENTIAL OILS ON PENICILLIUM EXPANSUM GROWTH

The questionable safety of food spoiled by microorganisms is and should be the subject of attention and investigation. Pathogens such as Penicillium expansum are more difficult to control due to their resistance and ubiquity. Awareness of plant essential oils (EOs) as alternative natural preservatives is increasing because of their significant antimicrobial activity. The aim of this research work was therefore to contribute to this topic with new results. The antifungal activity of twelve EOs was tested in vitro against five strains of Penicillium expansum, using the vapour-phase diffusion method. Three EOs, from bergamot, star anise and rosaline, inhibited colony growth during two-week cultivation with a partial efficacy, all other EOs showed complete (100%) inhibitory activity at a concentration of 625 μL.L-1. These (EOs from clove, cinnamon, cinnamon bark, laurel, lemongrass, peppermint, Mitcham mint, spearmint, and lavender) were included in the second part of the trial, the aim of which was to determine their minimum inhibitory doses (MIDs). In the case of peppermint, Mitcham mint, spearmint and lavender EOs, some strains of P. expansum grew already at a concentration of 500 μL.L-1. Clove EO stopped the fungal growth of two strains at 250 μL.L-1. Laurel EO was 100% effective at MID 250 μL.L-1 throughout the cultivation period. EOs from lemongrass and cinnamon bark had similar efficacy on the strains. Both EOs were able to inhibit one strain at a MID of 125 μL.L-1, cinnamon bark EO inhibited the remaining strains at 250 μL.L-1, lemongrass EO inhibited three strains at 250 μL.L-1 and one at 500 μL.L-1. The lowest MID was recorded for cinnamon EO, which effectively inhibited two strains at 125 μL.L-1, two strains at 62.5 μL.L-1 and one strain even at 31.25 μL.L-1. The sensitivity of the strains used in this study varied according to the EOs used.

Effects of Harvest Time and Hydrodistillation Time on Yield, Composition, and Antioxidant Activity of Mint Essential Oil.

In this study, we assessed the effects of different harvest times (9 a.m., 1 p.m., and 5 p.m.) and hydrodistillation times (60, 90, and 120 min) on the yield, chemical composition, and antioxidant activity of the spearmint (Mentha spicata L.) essential oil (EO) sourced from the Amazon region. EO yield was ≥1.55% and was not significantly influenced (p ≥ 0.05) by the different harvest times and hydrodistillation times. Thirty-one different organic compounds were identified, of which menthol (91.56-95.68%), menthone (0.6-2.72%), and isomenthone (0.55-1.46%) were the major constituents. The highest menthol content in the EO was obtained from samples collected at 9 a.m., with a hydrodistillation time of 60-90 min, compared to other harvest and hydrodistillation times. This suggests that exposure to sun and light, which is greater at harvest times of 1 p.m. and 5 p.m., decreased the menthol content and altered the chemical composition of Mentha EO. Furthermore, the sample harvested at 9 a.m. and hydrodistilled for 60 min showed the highest antioxidant activity (61.67 equivalent mg of Trolox per g of EO), indicating that antioxidant activity is strongly affected by light exposure and the contact duration of the sample with boiling water during hydrodistillation.

Kinetic analysis, mathematical modeling and quality evaluation of mint drying in greenhouse solar dryer

The goal of this study is to evaluate the potential of the Greenhouse Solar Dryer (GSD) for drying the mint leaves and compare it with other traditional drying methods. The top 10 mathematical models introduced by literatures were selected to describe the drying kinetics of mint leaves. The results showed that the average drying time in the GSD is 32 % and 50 % less than the open sun and shade place drying, respectively. Wang and Singh model with R2 = 0.9944, RMSE = 0.02021 and χ2 = 0.00052, logarithmic model with R2 = 0.9918, RMSE = 0.02556 and χ2 = 0.00078, and Henderson-Dabbie with model with R2 = 0.9774, RMSE = 0.04189 and χ2 = 0.00175 were determined as the best models for GSD, sun and shade place drying, respectively. The amount of changes in the color of the dried mint according to the index of total changes (ΔE) was recorded as 0.53 in the shade place, 0.60 in the GSD, and 0.89 in the open sun drying, respectively. The average of dried mint essential oil that was extracted by hydro-distillation method was 0.0254, 0.0237 and 0.0101 for shade place, GSD and open sun drying, respectively. Comparison of the average amount of extracted dried mint essential oil using ANOVA test, two LSD and Tukey HSD (Honestly Significant Difference) tests showed that there is no significant difference between the two treatments of drying in the shade place and GSD, while both of them have a significant difference with the open sun.