Bovine brucellosis, caused by Brucella abortus, is a significant bacterial infectious zoonotic disease not only poses a threat to animal health but also presents economic challenges and public health concerns due to its potential transmission to humans. Humans can contract the disease through direct contact with infected animals or consumption of unpasteurized dairy products. Bovine anaplasmosis is a haemolytic disease of cattle caused by the bacterium Anaplasma marginale which can cause adult mortality, abortion, weight loss, and a reduction in performance. This paper described the results obtained from the effects of brucellosis and anaplasmosis on serum proteins and the level of the macro elements (Calcium, Phosphorus, Potassium, Sodium, and Magnesium). These minerals play an essential role in animal metabolism. Out of fourteen Bovine serum samples, five samples were found to be positive for Brucella (35.72%) and four samples were found to be positive for Anaplasma (28.57%), the remaining samples were negative. Total protein (Tb) and (Alb) levels were significantly increased (P<0.01) in both brucellosis and anaplasmosis cattle compared with controls. On the other hand Globulin (Glob) was significantly decreased (P<0.01) in brucellosis cattle compared to the controls, also the present study showed that, concentration of Na, Ca, and Mg minerals were significantly increased in case of Brucellosis compared to the controls and significantly low (P<0.05) in potassium level, while animals infected with anaplasmosis showed an increased level of Na and K and decreased level in Mg. In summary, comprehension of laboratory medicine parameters is crucial for monitoring animal health and diagnosing diseases like brucellosis and anaplasmosis. These parameters are essential for implementing effective control measures and ensuring livestock well-being. The objective of this study was to estimate the total proteins and minerals content of Bovine Brucellosis and Anaplasmosis in ...