Mineral Deposits Research Articles

Classification of rare earths mineral resources using the Jequié/BA complex rock weathering chemical index

The most common economic mineral deposits of Rare Earths are associated to magmatic calc-alkaline to alkaline rocks and their products. This work presents data from drilling carried out in rocks of the Jequié/BA Complex, mineralized in rare earths, which were subjected to an intense weathering process, similar to what occurred in the Ion Adsorption Clays deposits in southern China. The interpretation of the survey data allowed the classification of rare earths mineral resources with similar, sometimes higher, levels than those of Chinese deposits, contained in the lateritic regolithic profile studied. The chemical index of rock alteration (CIA) was adopted as a classification tool, horizontally separating the mineralized weather profile into six zones. The data indicate that there was supergenic enrichment REO in the lower portions of the profile and sometimes in the upper zones. The average levels ranged between 0.09% and 0.22% REO, with maximum values of 1.6%. A model section representing the deposit of rare earths minerals of supergenic origin in rocks of the Jequié/BA Complex was developed.

Effects of phytase inclusion in diets containing rice protein concentrate (RPC) on the nutrient digestibility, growth and chemical characteristics of rohu (Labeo rohita).

The objective of the current study was to assess the impact of dietary phytase supplementation on Labeo rohita fingerlings and to examine the effects on growth, nutrient digestibility and chemical characteristics of diets containing rice protein concentrate (RPC) as a major protein source. Six experimental diets were made, i.e., a positive control (fishmeal-based diet with no phytase), FM0; a negative control (RPC-based diet with no phytase), RPC0; and four supplemental phytase levels (250, 500, 1000, and 2000 FTU/kg). Fingerlings with an average weight of 9.42 ± 0.02 grams (mean ± SD) were randomly distributed into six experimental groups of three replicates, each containing 25 fish per tank (75 liters of water), provided with experimental diets at a rate equivalent to 5% of their body weight for 90 days, and uneaten feed was collected after 2 hours to determine feed consumption. The feces were collected before feeding to estimate digestibility. Phytase in combination with the RPC-based diet significantly (p < 0.05) enhanced phytate phosphorus in vitro hydrolysis; growth performance; nutrient (crude protein, crude fat, moisture and gross energy) and mineral (P, Ca, Mg, Na, K, Zn, Mn and Cu) digestibility; digestive enzyme (protease, lipase and amylase) activity; and mineral deposition up to 1000 FTU/kg phytase. However, the hepatosomatic and viscerosomatic indices and carcass composition were not influenced (p > 0.05) by phytase supplementation. Increasing phytase supplementation in the RPC-based diets led to a significant (p < 0.05) decrease in the serum biochemical parameters (alkaline phosphatase activity, aspartate aminotransferase, alanine aminotransferase), which resulted in improved liver health. In conclusion, phytase-supplemented RPC-based diets improved the growth, mineral/nutrient digestibility, digestive enzymes, serum biochemistry, and mineral deposition of L. rohita fingerlings up to 1000 FTU/kg. Broken line regression analysis revealed that the optimum phytase concentration in the RPC-based diet for L. rohita was 874.19 FTU/kg.

Gold in eskers and gyttja overlying auriferous till, Regnault deposit, Quebec

The Regnault gold deposit in Quebec, Canada, was discovered by Kenorland in 2020 following identification of a gold-in-till dispersal train. In 2022, two non-traditional media—eskers and organic lake-bottom mud (gyttja)—were sampled to determine if they likewise contained gold anomalies that vectored the mineral deposit. Striking, coherent (mappable) anomalies exist in both media. The gold-in-esker dispersal train has a similar pathfinder element association as the gold-in-till dispersal train (e.g., Te, W) but contains approximately twice the gold (average 73 ppb Au) in the &lt;63 um fraction. It is hypothesized to be a meltwater-sorted version of the gold-in-till train, sourced from the erosional esker corridor some 3 km upflow. By contrast, the gold-in-gyttja dispersal train is substantially different than both the gold-in-esker and gold-in-till dispersal trains. It has lost significant association with pathfinder elements, contains an order of magnitude less gold (average 4.2 ppb Au), and is tentatively hypothesized to have a hydromorphic origin, sourced primarily from the gold-in-till dispersal train. The main takeaway from the study is that the sampling of any of these three media—eskers, gyttja or till—could have conceivably led to the discovery. Supplementary material: https://doi.org/10.6084/m9.figshare.c.7235784

A Review of Relationship between the Metallogenic System of Metallic Mineral Deposits and Lithospheric Electrical Structure: Insight from Magnetotelluric Imaging

In development over 70 years, magnetotelluric (MT) sounding, a high-resolution technique for subsurface electrical resistivity imaging, has been widely applied in resource exploration in the Earth. The key factors of the metallogenic system of metallic mineral deposits can be closely correlated to the electrical anomalies of the lithosphere. In this paper, we review the relationship between the electrical resistivity model of the lithosphere and the metallogenic system. At the beginning, we indicate why the electrical parameters relate to the metallogenic system in all geophysical parameters. The advantage of MT sounding in sketching an electrical resistivity model of the lithosphere is subsequently discussed, and some methods of data processing, analysis and inversion are also introduced. Furthermore, we summarize how to bridge the relationship between the electrical resistivity model of the lithosphere and metallogenic system, and analyze the influence of the rheological variation estimated from conductivity in the lithosphere on mineralization. In the end, we list some typical cases of the application of MT sounding in mineral exploration, and also give some suggestions for future work. This study is aimed at providing guidance in discussing the metallogenic system using an electrical resistivity model.

#1299 The SGLT2 inhibitor canagliflozin, but not dapagliflozin or empagliflozin, ameliorates vascular calcification in a mouse model of nephrocalcinosis

Abstract Background and Aims Vascular calcification, defined as the abnormal deposition of minerals in blood vessels, is a major cardiovascular risk factor in patients with kidney failure. Sodium-glucose co-transporter 2 (SGLT2) inhibitors, commonly known as gliflozins, are a class of pharmaceutical compounds that have revolutionised the therapeutic management of type 2 diabetes. These drugs target the SGLT2 transporter, increasing glucose elimination through the urine, effectively lowering hyperglycaemia. In addition to their effect on blood sugar control, SGLT2 inhibitors have recently gained attention for potential cardiovascular benefits in patients with type 2 diabetes, chronic kidney disease (CKD) or established cardiovascular disease. Initially thought to be secondary to the promotion of urinary sodium and glucose excretion and weight loss, recent studies have also reported direct effects of gliflozins on the vascular compartment. Given the significant impact of vascular calcification on cardiovascular health in patients with kidney failure, the aim of this study was to investigate whether the three different SGLT2 inhibitors (canagliflozin, empagliflozin and dapagliflozin) could reduce the development of vascular calcification. Method Western blotting was used to investigate SGLT2 expression in mouse and human vascular smooth muscle cells (VSMC) and vascular explants. Human and mouse aortic VSMC and mouse aorta and human epigastric and iliac artery segments were treated with calcification medium supplemented with 1 or 5 µM of gliflozins. A nephrocalcinosis mouse model consisting of 2 weeks of high phosphorus diet (2%) in female DBA/2 mice was used in in vivo experiments. The animals were treated daily with the different gliflozins (3 mg/kg/j) by oral gavage. Calcium deposits were quantified using a quantitative colorimetric assay and alizarin red staining, blood and urine glucose levels were measured using a quantitative colorimetric assay and glomerular filtration rate (GFR) was measured using sinistrin-FITC excretion. Results We observed that SGLT2 is expressed in mouse and human vessels and vascular smooth muscle cells (VSMC). Treatment with canagliflozin, but not dapagliflozin or empagliflozin, reduced calcification in vitro, in human and mouse VSMC and ex vivo, in mouse aorta and human epigastric and iliac artery segments. The high phosphorus diet in mice led to nephrocalcinosis associated with impaired renal function as well as vascular calcification development in the aorta. In this model, although all 3 gliflozins showed efficacy in increasing urinary glucose excretion, blood glucose levels were not affected by gliflozins and renal function was not improved. However, canagliflozin, but not dapagliflozin or empagliflozin, significantly reduced vascular calcification in this nephrocalcinosis mouse model. Conclusion Our study demonstrated that canagliflozin reduces vascular calcification development, highlighting its potential as a therapeutic strategy. This protective effect was found to be drug-dependent and not class-dependent, as the other two SGLT2 inhibitors, dapagliflozin and empagliflozin, showed no significant effect on vascular calcification. Further studies are needed to understand the mechanisms and pathways involved and to determine whether the differential effect of the gliflozins is due to an off-target action of canagliflozin on other SGLT transporters.

Droplet bioprinting of acellular and cell-laden structures at high-resolutions

Advances in digital light projection(DLP) based (bio) printers have made printing of intricate structures at high resolution possible using a wide range of photosensitive bioinks. A typical setup of a DLP bioprinter includes a vat or reservoir filled with liquid bioink, which presents challenges in terms of cost associated with bioink synthesis, high waste, and gravity-induced cell settling, contaminations, or variation in bioink viscosity during the printing process. Here, we report a vat-free, low-volume, waste-free droplet bioprinting method capable of rapidly printing 3D soft structures at high resolution using model bioinks and model cells. A multiphase many-body dissipative particle dynamics model was developed to simulate the dynamic process of droplet-based DLP printing and elucidate the roles of surface wettability and bioink viscosity. Process variables such as light intensity, photo-initiator concentration, and bioink formulations were optimized to print 3D soft structures (∼0.4–3 kPa) with a typical layer thickness of 50 µm, an XY resolution of 38 ± 1.5 μm and Z resolution of 237 ± 5.4 µm. To demonstrate its versatility, droplet bioprinting was used to print a range of acellular 3D structures such as a lattice cube, a Mayan pyramid, a heart-shaped structure, and a microfluidic chip with endothelialized channels. Droplet bioprinting, performed using model C3H/10T1/2 cells, exhibited high viability (90%) and cell spreading. Additionally, microfluidic devices with internal channel networks lined with endothelial cells showed robust monolayer formation while osteoblast-laden constructs showed mineral deposition upon osteogenic induction. Overall, droplet bioprinting could be a low-cost, no-waste, easy-to-use, method to make customized bioprinted constructs for a range of biomedical applications.

Peculiarities of the development and reproduction of the Russian lead and zinc mineral resource base and its forecasting for up to 2040

The state and structure of reproduction of the lead and zinc mineral resource base are shown for the period of 1993-2022, and a forecast of its development for up to 2040 is presented. The mineral reserves movement and redemption were analyzed with account made for the mineral deposit types. The risks and limitations were identified that affected the mineral resource base sustainability. The need is justified to increase the early phase geological exploration at the expense of the federal budget funds to prepare the forecasted resources and to create an “exploration reserve” for lead and zinc.

Inferring arsenic anomalies indirectly using airborne hyperspectral imaging – Implication for gold prospecting along the Rise and Shine Shear Zone in New Zealand

Well-exposed mineral deposits are scarce at a global level and presently potential mineral-rich sites are underlying vegetation cover and topsoil, which are suboptimal for direct remote sensing based exploration techniques. This study aims to implement an indirect approach to arsenic (As) distribution mapping using the surface manifestations of the subsurface geology and link it to the known gold mineralisation in the study area. Rise and Shine Shear Zone (RSSZ) in New Zealand is broadly a part of the Otago schist hosting lower to upper green-schist facies rocks manifesting mesothermal gold mineralisation. The area has several surficial geological imprints separating mineralised and non-mineralised zones, but these are dominated by topographic ruggedness, soil moisture and vegetation (mainly grass/tussock) spectra in the hyperspectral data. Initially, a band selection using Recursive Feature Elimination (RFE) was executed. The bands generated were tallied with the field and geological understanding of the area. The resultant 85 bands were then further put through Orthogonal Total Variation Component Analysis (OTVCA) to concise the information in 10 bands. OTVCA output was then classified using Random Forest classifier to map three levels of As concentration (<20 ppm, between 20 and 100 ppm and >100 ppm). The potentially high As concentration zones are likely to be related to the gold mineralisation. The geology of the area correlates with soil exposure which is captured by the classification in some parts, this increases the accuracy but also makes the classification analysis challenging.

Numerical Simulation of Chemical Reactions’ Influence on Convective Heat Transfer in Hydrothermal Circulation Reaction Zones

Chemical reactions, mineral diffusion, and deposition are pivotal in understanding the mechanisms of mineral deposition and the formation of seafloor sulfides in the hydrothermal circulation process. To understand the formation process of anhydrite in submarine hydrothermal systems, a computational model that combined component transport and chemical reactions was established and simulated using the mass transport model. The deposition rate of calcium sulfate was defined, and the effects of factors such as porosity, ion concentration, and inflow velocity on the temperature field in the reaction zone were thoroughly investigated. The distribution of temperature, porosity, and velocity during the reaction process was obtained, allowing for the identification of the chemical reaction patterns of certain ions in the early stages of hydrothermal activity. The simulation revealed the occurrence of biochemical reactions between two types of ions, leading to their deposition on the solid framework of a porous medium. With the increase in inflow velocity and solute concentration, the average porosities of the porous medium decreased by 0.495% and 0.468%, respectively, which consequently altered the structure of the rock. Such findings contribute to the inference of formation and extinction mechanisms of seafloor crusts and hydrothermal chimneys.

Sociocultural risks of resource extraction for the low-carbon energy transition: Evidence from the Global South

The ongoing global transition to low-carbon energy relies on access to energy transition minerals (ETMs), bringing to life new mining projects. This shift is particularly impacting the Global South, due to high levels of interaction of mineral deposits with territories of Indigenous peoples and peasants, weak governance and remoteness. Focusing on the sustainability and justice of energy transitions, previous research has identified a range of environmental and social impacts of ETM mining across Global South countries. We argue that current research overlooks impacts of energy transition-related mining on tangible and intangible cultural heritage of land-connected people. This paper seeks to address this gap by examining the intersection of ETM mining and sociocultural practices in the Global South from spatial, governance and justice perspectives. Based on an extensive literature review, this article critically addresses key themes in the literature on the social aspects of energy transitions and uses cases from Ghana, the Democratic Republic of Congo (DRC), Chile, and Papua New Guinea (PNG) to demonstrate the immediate and long-term sociocultural impacts associated with ETM mining. It is important to foster an all-encompassing approach to planetary just energy transitions that places equal weight on ensuring cultural alongside environmental, economic and social sustainability.

Fibroblasts’ secretome from calcified and non-calcified dermis in Pseudoxanthoma elasticum differently contributes to elastin calcification

Pseudoxanthoma elasticum (PXE) is a rare disease characterized by ectopic calcification, however, despite the widely spread effect of pro/anti-calcifying systemic factors associated with this genetic metabolic condition, it is not known why elastic fibers in the same patient are mainly fragmented or highly mineralized in clinically unaffected (CUS) and affected (CAS) skin, respectively. Cellular morphology and secretome are investigated in vitro in CUS and CAS fibroblasts. Here we show that, compared to CUS, CAS fibroblasts exhibit: a) differently distributed and organized focal adhesions and stress fibers; b) modified cell-matrix interactions (i.e., collagen gel retraction); c) imbalance between matrix metalloproteinases and tissue inhibitor of metalloproteinases; d) differentially expressed pro- and anti-calcifying proteoglycans and elastic-fibers associated glycoproteins. These data emphasize that in the development of pathologic mineral deposition fibroblasts play an active role altering the stability of elastic fibers and of the extracellular matrix milieu creating a local microenvironment guiding the level of matrix remodeling at an extent that may lead to degradation (in CUS) or to degradation and calcification (in CAS) of the elastic component. In conclusion, this study contributes to a better understanding of the mechanisms of the mineral deposition that can be also associated with several inherited or age-related diseases (e.g., diabetes, atherosclerosis, chronic kidney diseases).

POLLUTION OF GROUNDWATER RESOURCES BY PROCESSES IRON ORE SURFACE USE

In Ukraine, as in most countries of the world, preservation of the natural qualities of surface and underground water resources is one of the difficult to solve problems. Groundwater pollution is carried out spontaneously and as a result of organized emissions on the surface of territories and into underground cavities. Contaminated areas of territories around mineral deposits, industrial facilities, as well as masses of pollutants, are growing. In terms of scale, it is necessary to single out groundwater pollution as a result of ore extraction and placement of rocks in dumps, as the most harmful for agricultural lands and surface water bodies. Calculating the amount of compensation for damages caused by contamination of groundwater with harmful substances requires further official adjustments, taking into account the factor of public ownership of natural resources in general. Therefore, it is necessary that the following situations are officially taken into account in the normative legal acts of local authorities: underground waters and their environment belong to objects of public property, therefore, in the methods of determining and calculating the ecological consequences of pollution of underground waters and their underground environment, it is necessary to provide that damages must be studied and assessed from the point of view of damage to public property; polluted underground waters and their underground environment are located locally on the territories of territorial Starostov districts, which represent the people of Ukraine; in accordance with clause 1, when calculating the amount of compensation for pollution, it is generally necessary to determine the losses for the population of these districts and the amounts of charges and payments to them; local authorities need to organize compensation processes, monitor their implementation and effectiveness. The definition of an environmental tax does not correspond to the definition of the concept of a tax as an unconditional payment to the budget, since in its essence it is a punishment for the discharge of polluted substances into water bodies and ecological pollution of water resources.

Enamel Remineralisation with a Novel Sodium Fluoride-Infused Bristle Toothbrush.

This study aims to investigate whether toothbrushes with fluoride-infused bristles have any (re)mineralisation effects on bovine enamel. Bovine incisors (N = 160) were extracted, and the buccal side of the crown was cut into dimensions of ~5 mm × 5 mm with a low-speed saw. These specimens were randomly allocated into four groups: half (80 teeth) were stored in demineralising solution (DM), and the other half were stored in deionised water (DW) for 96 h. Then, they were brushed with a force of 2.0 ± 0.1 N for five min with a manual toothbrush with either fluoride-infused (TF) or regular (TR) bristles. Microhardness (Vickers), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM) were used to investigate the surfaces of the bovine enamel specimens before and after brushing. Two-way ANOVA was used to analyse the hardness data, and the pairwise comparison method was used to analyse the Ca/P ratio, for each group at α = 0.05. The results show that brushing with either of these toothbrushes increased the Vickers microhardness on DM and DW enamel (p < 0.001), whereas hydroxyapatite was revealed in all groups by XRD. The DM samples showed a significant increase (p < 0.05) in the Ca/P ratios after brushing with TR and TF. Conversely, under DW conditions, these ratios decreased significantly after brushing. In terms of the F atomic%, TF increased significantly. SEM revealed mineral deposition in the DM groups after toothbrushing. To conclude, toothbrushing effectively induces the microhardness of sound and demineralised enamel, while fluoride-infused bristles might be able to retain fluoride on the enamel surface.

Economic evaluation of mineral deposit: A bottom-up approach

Proper economic evaluation of a mineral deposit is critical to effective investment decision-making in a mineral project. However, this often requires detailed mine production scheduling to produce a schedule that reflects the actual cash flow when the project comes on stream. Because of the dexterity required for this task many mine planners and explorationist attempt to use statistical formulas that approximate the mine scheduling and value of the project. The mathematical model developed for production scheduling often produces a constant production rate schedule over an approximate life span of the project. In this paper, we have attempted to apply a bottom-up approach that begins with geometrical modelling and equipment deployment pattern to define the number of equipment required for each sequence of operation based on available workfront in the development of each bench. Then, based on the number of equipment and the production rate at each sequence of operations, a production schedule is developed. This production schedule therefore will reflect annual cash flow since it is based on the sequence of operation. Application of this method on the west pit of Itakpe mine shows a considerable net present value and internal rate of return of the deposit compared with the evaluation made using statistical models. The NPV of the west pit was found to be USD621 million as against USD122.41 million using Nwosu’s formula and USD123.85 million Taylor’s formula. The above value of NPV using the proposed method shows the maximum expected NPV of the mineral project-based technical restrictions. An understanding of this value can guide the mineral property owner in decision-making.

Inorganic Biomaterials Shape the Transcriptome Profile to Induce Endochondral Differentiation.

Minerals play a vital role, working synergistically with enzymes and other cofactors to regulate physiological functions including tissue healing and regeneration. The bioactive characteristics of mineral-based nanomaterials can be harnessed to facilitate in situ tissue regeneration by attracting endogenous progenitor and stem cells and subsequently directing tissue-specific differentiation. Here, cellular responses of human mesenchymal stem/stromal cells to traditional bioactive mineral-based nanomaterials, such as hydroxyapatite, whitlockite, silicon-dioxide, and the emerging synthetic 2D nanosilicates are investigated. Transcriptome sequencing is utilized to probe the cellular response and determine the significantly affected signaling pathways due to exposure to these inorganic nanomaterials. Transcriptome profiles of stem cells treated with nanosilicates reveals a stabilized skeletal progenitor state suggestive of endochondral differentiation. This observation is bolstered by enhanced deposition of matrix mineralization in nanosilicate treated stem cells compared to control or other treatments. Specifically, use of 2D nanosilicates directs osteogenic differentiation of stem cells via activation of bone morphogenetic proteins and hypoxia-inducible factor 1-alpha signaling pathway. This study provides insight into impact of nanomaterials on cellular gene expression profileand predicts downstream effects of nanomaterial induction of endochondral differentiation.

Interpretable Machine Learning for Geochemical Anomaly Delineation in the Yuanbo Nang District, Gansu Province, China

Machine learning (ML) has shown its effectiveness in handling multi-geoinformation. Yet, the black-box nature of ML algorithms has restricted their widespread adoption in the domain of mineral prospectivity mapping (MPM). In this paper, methods for interpreting ML model predictions are introduced to aid ML-based MPM, with the goal of extracting richer insights from the ML modeling of an exploration geochemical dataset. The partial dependence plot (PDP) and accumulated local effect (ALE) plot, along with the SHAP value analysis, were utilized to demonstrate the application of random forest (RF) modeling within both regression and classification frameworks. Initially, the random forest regression (RFR) model established the relationship between the concentrations of Au and those of elements such as As, Sb, and Hg in the study area, and from this model, the most important geochemical elements and their quantitative relationships with Au were revealed by their contributions in the modeling through PDP and ALE analyses. Secondly, the RF classification modeling established the relationships of mineralization occurrences (i.e., known mineral deposits) with geochemical elements (i.e., Au, As, Sb, Hg, Cu, Pb, Zn, and Ag), as did RFR modeling. The most important geochemical elements for indicating regional Au mineralization and the trajectories of PDP and ALE reached a consensus that As and Sb contributed the most, both in the regression and classification modeling, with regard to Au mineralization. Finally, the SHAP values illustrated the behavior of the training samples (i.e., known mineral deposits) in RF modeling, and the resulting prospectivity map was evaluated using receiver operating characteristics.

The Species Effect: Differential Sphingosine-1-Phosphate Responses in the Bone in Human Versus Mouse.

The deterioration of osteoblast-led bone formation and the upregulation of osteoclast-regulated bone resorption are the primary causes of bone diseases, including osteoporosis. Numerous circulating factors play a role in bone homeostasis by regulating osteoblast and osteoclast activity, including the sphingolipid-sphingosine-1-phosphate (S1P). However, to date no comprehensive studies have investigated the impact of S1P activity on human and murine osteoblasts and osteoclasts. We observed species-specific responses to S1P in both osteoblasts and osteoclasts, where S1P stimulated human osteoblast mineralisation and reduced human pre-osteoclast differentiation and mineral resorption, thereby favouring bone formation. The opposite was true for murine osteoblasts and osteoclasts, resulting in more mineral resorption and less mineral deposition. Species-specific differences in osteoblast responses to S1P were potentially explained by differential expression of S1P receptor 1. By contrast, human and murine osteoclasts expressed comparable levels of S1P receptors but showed differential expression patterns of the two sphingosine kinase enzymes responsible for S1P production. Ultimately, we reveal that murine models may not accurately represent how human bone cells will respond to S1P, and thus are not a suitable model for exploring S1P physiology or potential therapeutic agents.

REPROGRAMMING FILAMENTOUS FD VIRUSES TO CAPTURE COPPER IONS.

C-terminal truncated variants (A, VA, NVA, ANVA, FANVA and GFANVA) of our recently identified Cu(II) specific peptide "HGFANVA" were displayed on filamentous fd phages. Wild type fd-tet and engineered virus variants were treated with 100 mM Cu(II) solution at a final phage concentration of 1011vir/ml and 1012vir/ml. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) imaging before Cu(II) exposure showed»6-8 nm thick filamentous virus layer formation. Cu(II) treatment resulted in aggregated bundle-like assemblies with mineral deposition. HGFANVA phage formed aggregates with an excessive mineral coverage. As the virus concentration was 10-fold decreased, nanowire-like assemblies were observed for shorter peptide variants A, NVA and ANVA. Wild type fd phages did not show any mineral formation. Energy dispersive X-ray spectroscopy (EDX) analyses revealed the presence of C and N peaks on phage organic material. Cu peak was only detected for engineered viruses. Metal ion binding of viruses was next investigated by enzyme-linked immunosorbent assay (ELISA) analyses. Engineered viruses were able to bind Cu(II) forming mineralized intertwined structures although no His (H) unit was displayed. Such genetically reprogrammed virus based biological materials can be further applied for bioremediation studies to achieve a circular economy.

Response characteristics and detectability of pegmatitic rare-metal deposits using different grounded-wire configurations

The differential electric dipole (DED) and differentially normalized electromagnetic method (DNME) have a stronger transverse magnetic field than the horizontal electric dipole (HED) and, thus, have been successfully used for resource exploration in conductive ocean environments. However, which of these two is more suitable for the exploration of resistive targets on land, such as pegmatite veins and ore-related mafic and ultramafic intrusions in mineral deposits, remains unknown. Thus, we conducted a comparative study of the DED, DNME, and HED based on forward modeling and application to a field survey. The signal amplitude of the horizontal electric field measured using the DED and DNME was substantially weaker than that obtained via the HED with the same source length and transmitting current. The lateral variation in the horizontal electric field response observed via the DNME reflected the change in the shallow electrical structure, indicating the spatial distribution of pegmatite veins, as verified by the 3D simulation results and data obtained in a field survey of pegmatitic rare-metal deposits in Lushi County. The DED and DNME exhibited a similar ability for detecting resistive targets. Both methods showed a higher detectability than HED, based on 3D synthetic modeling. Our results indicate that the DNME, with its higher responses and better lateral resolution, is a better choice in field surveys on land than DED.

Enhancement of bone mineral formation on Ti6Al4V surface by PM-EDCLT combined with acid etching

For Bone-anchored limb prostheses, osseointegration plays a significant role in the mechanically stable interface between bone and implants. Titanium alloy is the most usable alloy for hard tissue replacement. The drawbacks of the titanium implant are corrosion, leaching and poor osseointegration. In this research, powder mixed (hydroxyapatite) - electric discharge-assisted centreless turning (PM-EDCLT) (Impulse current- 5amp, duty factor- 57%, hydroxyapatite concentration 18 g/l, and rpm-360) and acid etching (solution – HF: HNO3: H2O = 1:2:50) were used for the surface modification of screw-fit implants. Average surface roughness value maximum in PM-EDCLT machined surface (1.249 µm) followed by PM-EDCLT machined + 5 min. Acid etching (1.055 µm), PM-EDCLT machined + 10 min. Acid etching (0.9829 µm), PM-EDCLT machined + 15 min. acid etching (0.8223 µm), bare surface with 10 min. Acid etching (0.2866 µm), 15 min. acid etching (0.2714 µm) and 5 min. Acid etching (0.1896 µm). Investigations using Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FTIR) proved that the deposited layer on the powder-mixed EDCLT machined sample was hydroxyapatite. After sinking samples in the Simulated Body Fluid (SBF) solution, it was concluded that the percentage of bone minerals like oxide, Ca, and P deposition increases after increasing the acid etching time on the PM-EDCLT machined surfaces. Little effect was found on the deposition of bone minerals on the surface of acid etching only by increasing the etching time. There is a clear trend of decreasing the contact angle with increasing the acid etching time on the PM-EDCLT surfaces.