Min Extraction Time Research Articles

Efficient Simultaneous Isolation of Pinostrobin and Panduratin A from Boesenbergia rotunda Using Centrifugal Partition Chromatography.

The bioactive flavonoids pinostrobin (PN) and panduratin A (PA) from Boesenbergia rotunda are essential for research and therapeutic applications. This study introduces an innovative method utilizing ultrasound-assisted extraction with n-hexane pre-treatment, followed by one-step centrifugal partition chromatography (CPC) purification. Extraction efficiency was evaluated using ultra high-performance liquid chromatography (UHPLC), and the isolated compounds were characterized through 1H-NMR and liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), adhering to AOAC validation guidelines. Optimal extraction conditions comprised a particle size of 125 μm, a solid-to-liquid ratio of 1:30 g/mL, and a 10 min extraction time, yielding a crude extract of 6.96 ± 0.07%. Using an n-hexane/MeOH/water (5/3.4/1.6, v/v) solvent system in ascending mode, PN (2.16 mg, 98.78% purity) and PA (0.4 mg, 99.69% purity) were isolated from 67 mg of crude extract within 30 min. This streamlined approach enhances purification efficiency, allowing for faster extraction and higher purity, making it a suitable method for commercial applications.

Preparation of a novel organic-inorganic composite sludge bioflocculant (SBF) from dewatered sludge as raw material: Characteristics, flocculation mechanism and application for domestic sewage

In this work, a green sludge bioflocculant (SBF) was prepared via chemical hydrolysis of dewatered sludge and applied to flocculation of domestic wastewater. The process parameters for the preparation of the SBF were 1.80 % hydrochloric acid concentration, 60 min extraction time, and 4000 r/min centrifugation speed. SBF is polymeric flocculant composed of organic and inorganic compounds. Flocculation efficiency reached 97.31 ± 0.26 % under optimal flocculation conditions. Charge neutralization promotes the surface adsorption, bridging and net trapping and sweeping of Fe (OH)3, Al (OH)3 and active functional groups OH/NH and C = O in SBF, which together achieve efficient flocculation reactions. SBF had high efficiency and stable flocculation performance for phosphorus in urban domestic wastewater, and the concentration of TP in effluent was lower than 0.30 mg/L. Therefore, SBF prepared from dewatered sludge has efficient flocculation properties and is suitable for removing pollutant phosphorus, which has good application prospects in the field of wastewater treatment.

High-intensity ultrasound-assisted alkaline extraction of soy protein: Optimization, modeling, physicochemical and functional properties

This study examined the effect of high-intensity ultrasound-assisted alkaline extraction (HUAE) on the extraction yield and the physicochemical and functional properties of soy protein (SP) using the two-pot multivariate method for the first time. Plackett-Burman Design (PBD) coupled with Response Surface Methodology (RSM) was systematically utilized to select and subsequently optimize the HUAE parameters. Based on PBD results, the significant extraction factors were liquid to solid ratio (LSR), temperature, ultrasonic amplitude, and extraction time. The optimum conditions for the maximal extraction yield and minimal energy consumption were 50:1 mL/g LSR, 50 °C, 48 % ultrasonic amplitude, and 10 min extraction time. At optimum conditions, the extraction yield (35.28 %) was significantly improved compared to traditional extraction (26.39 %). Besides, HUAE resulted in modification of the protein secondary and tertiary structures due to the unfolding of protein molecules and the exposure of hydrophobic groups or regions as shown by FTIR spectroscopy, free sulfhydryl analysis, and scanning electron microscopy. These structural changes led to decreased solubility and emulsifying activity but improved emulsion stabilization and antioxidant properties. With future development, HUAE could potentially produce soy protein for targeted applications, broadening its utilization and meeting the need for more sustainable alternative processing.

Orange peel: Low cost agro-waste for the extraction of polyphenols by statistical approach and biological activities

Food waste is an excellent source of various bioactive secondary metabolites. In addition, food waste is a global issue, and more research is being focused on the conversion of value-adding products from food waste, especially fruits. In this study, bioactive polyphenols were extracted from orange peels. The parameters that affect the extraction of polyphenols from orange peels were optimized using response surface methodology. Ethanol was used to extract bioactive polyphenols, and the extraction time (min), extraction temperature, and ethanol concentration (%) were analyzed. Orange peels are an excellent source of polyphenols, and the yield was increased under optimized extraction conditions (p<0.05). The polyphenol content increased twofold (38.4 mg GAE/g) under the optimized extraction conditions (53% ethanol, 51 degrees centigrade and 96 min extraction time). Crude polyphenols exhibited significant antibacterial activity against Bacillus subtilis (19±1 mm zone of inhibition), followed by Staphylococcus aureus (17±2 mm zone of inhibition) (p<0.05). In addition, orange peel extract exhibited antifungal activity against A. flavus and C. albicans. The ethanol extract exhibited DPPH activity (1.61±0.03 mg GAE/100 g) and ABTS reducing power (1.48±0.01 mg GAE/100 g). The polyphenol compounds exhibited antibiofilm activity against biofilm-producing Bacillus subtilis and increased in a dose-dependent manner (p<0.05).

Valorization of Pineapple Core Waste for Sequential Extraction of Phenolic Compounds and Carotenoids: Optimization Through Ultrasound-Assisted Method and Box–Behnken Design

AbstractIn this study, a pioneering cascade method involving ultrasound-assisted extraction (UAE) and Box–Behnken Design (BBD) was optimized to valorize pineapple core waste by the sequential extraction of, firstly phenolic compounds and, secondly, carotenoids. The effectiveness of the extraction was evaluated based on total polyphenol content (TPC) and antioxidant activity using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. Characterization of the carotenoids was performed using high-performance liquid chromatography with a diode array detector (HPLC–DAD). The initial characterization of dry pineapple core (DPC) samples revealed their nutritional composition, including protein, lipid, and carbohydrate weight percentages of 1.20 ± 0.05%, 5.3 ± 0.4%, and 88.6 ± 0.5%, respectively. The high extractives content (40.0 ± 4.5%) suggests a substantial presence of phenolic compounds, making the pineapple core a valuable source of natural antioxidants. The optimal UAE conditions for phenolic compound extraction were 70% amplitude, 5 min extraction time, and 2 cycles, yielding an antioxidant extract rich in phenolic compounds with a desirability value of 81.2%. Therefore, DPC was considered a valuable source of natural antioxidants. The extraction of β-carotene also showed promising results with optimal UAE conditions of 20% amplitude, 3 min extraction time, and 2 cycles. This research promotes the sustainable use of pineapple waste and demonstrates the potential to obtain valuable additives for the food, pharmaceutical, and cosmetic industries, encouraging a more circular and efficient use of resources in the pineapple processing industry.

Ultrasound Assisted Extraction of Bioactive Compounds Rich Extract from Immature Dropped Kinnow Fruit

Ultrasound-assisted extraction process parameters of bioactive compounds extraction from immature dropped kinnow fruit were optimized using response surface methodology. Box–Behnken design of the experiments was used to optimize the independent extraction variables: amplitude (30% to 70%), extraction time (3 to 9 min), and pulse cycle (2 to 6 s) for maximizing extraction of bioactive (total phenolics, total flavonoids, antioxidant activity such as 2, 2-diphenyl-1-picrylhydrazyl (DPPH) inhibition and ferric reduction activity potential (FRAP) activity) compounds from immature dropped kinnow fruits. Optimized conditions for ultrasonication bioactive extraction using 45.80% amplitude, 6.63 min extraction time and 4.24 s pulse cycle yielded 5.02 g GAE 100g-1 total phenolics, 9.41 g QE 100g-1 flavonoid content, 302.88 mg AAE 100g-1 DPPH, 6.95 g TE 100g-1 FRAP activity, 0.76 g DE 100g-1 saponins and 6.29 mg AE 100g-1 alkaloids. These optimized conditions could be used for pilot scale extraction of bioactive compounds from immature dropped kinnow fruits.

Application of deep eutectic solvent based dispersive liquid–liquid microextraction method followed by HPLC-DAD for quantification of selected neonicotinoid pesticides in vegetable oils

The use of deep eutectic solvents (DES) as alternative green solvents in various analytical chemistry fields has garnered attention of researchers globally. Therefore, this study presents environmentally friendly DES for extraction of selected neonicotinoids (acetamiprid, imidacloprid, thiacloprid and thiamethoxam) prior to their determination using high-performance liquid chromatography- diode array detector (HPLC-DAD). The DES mixture that resulted in the highest extraction recoveries of neonicotinoids (NEOs) was choline chloride with ethylene glycol (CCl:EG) and nuclear magnetic resonance and Fourier transform infrared confirmed the successful synthesized CCl:EG. Thereafter, the CCl:EG was applied in dispersive liquid–liquid microextraction (DLLME) for the preconcentration and extraction of acetamiprid, imidacloprid, thiacloprid and thiamethoxam. The optimum factors affecting extraction efficiency of the DES-DLLME procedure were 0.8 mL (DES volume), 4.5 min (extraction time), 0.5 mL (eluent volume), 7 pH and 75 s (eluent time). The optimized DES-DLLME achieved limit of detection (LOD) and quantification (LOD) range of 0.4–4.95 ng.µL−1 and 1.43–9.70 ng.µL−1 respectively. Additionally, the extraction method achieved good accuracy (79.0–119.6 %), preconcentration factors (39.3–118), interday (0.61–1.62 %) and intraday (0.1–0.98 %) precisions. The greenness assessment of the proposed DES-DLLME method using AGREE, NEMI and AES tools revealed that the method was green. Finally, the developed DES-DLLME method was applied to real vegetable oils and the concentrations were below detection limits.

A novel deep eutectic solvent-based green extraction and purification of DPP-IV inhibitory peptides from tilapia (Oreochromis niloticus) viscera hydrolysate

The use of green and low-cost techniques to produce bioactive peptides with anti-diabetic properties from protein-rich fish waste is a sustainable way to manage agro-fishery and food industrial waste. This study investigated a novel ultrasound (UL) assisted deep eutectic solvent (DES) based extraction approach to recover bioactive protein-rich extracts from tilapia fish viscera waste under optimized conditions (52.52% ultrasound amplitude, 1:4 DES molar ratio, 25 min extraction time). The aqueous two-phase system (ATPS) method successfully concentrated the crude proteins in the top phase and back-extracted proteins to the bottom phase with a high recovery yield. Hydrolysis by Alcalase produced tilapia viscera protein hydrolysate (TVPH) with low molecular weight (MW) peptides and low dipeptidyl peptidase (DPP)-IV half-maximal inhibitory concentration (IC50) value of 1.57 ±0.16 mg/mL. Notably, TVPH-3 fraction and TVPH-3-2 sub-fraction exhibited appreciable DPP-IV IC50 values of 1.36 ±0.08 mg/mL and 0.42 ±0.01 mg/mL, respectively. Ultrafiltration combined with RP-HPLC enriched the DPP-IV inhibitory concentration of the individual protein fractions. TVPH-3-2 sub-fraction contained hydrophobic amino acids and peptide sequences with typical DPP-IV inhibitory peptide sequence motifs. The results presented in this study indicate that tilapia viscera biomass is a potential source for protein recovery aided with a green extraction approach, and tilapia viscera protein-derived hydrolysates have potential applications as a potent functional food ingredient for the management of metabolic diseases like type-2 diabetes.

Response Surface Optimization of the Extraction Process and the Antioxidant Capacity of Total Flavonoids from Microcos Paniculata L.

Ultrasonic-microwave synergistic method was used to extract the total flavonoids from Microcos Paniculata L. To optimize the extraction process, we have conducted single-factor experiments on the total flavonoids yield to provide a basis for analysis. After the results were evaluated, we carried out the response surface method to test the antioxidant properties of total flavonoids in Microcos Paniculata L. The evaluation of antioxidant capacity was done from four dimensions. According to the experiments, the optimal extraction parameters included: 40 mesh particle size, 20:1 liquid/feed ratio (mL/g), 250 W ultrasonic power, 400 W microwave power, 30 min extraction time, and a 60% volume fraction of ethanol. When all the conditions were met, the yield of total flavonoids from Microcos Paniculata L. could reach 6.01%. The total flavonoids from Microcos Paniculata L. could remove DPPH.and ABTS+. At the same mass concentration, the scavenging rates. were as high as 93.14% and 84.40%, respectively. They were close to the scavenging rates of Vc in the positive control group. This proves that the total flavonoids in Microcos Paniculata L. have a good antioxidant ability.

Natural deep eutectic solvent-based dispersive liquid-liquid microextraction of pesticides in drinking waters combined with GC-MS/MS detection

The current research aims to develop a new analytical method applying a dispersive liquid-liquid microextraction (DLLME) assisted by vortex and using an environmentally friendly extractant for the preconcentration of organochlorine and organophosphorus pesticides followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis. The extractant (i.e., natural deep eutectic solvent (NADES)) is safe, cheap, biodegradable and can be prepared by simply mixing DL-menthol and decanoic acid (molar ratio 2:1). The main experimental factors affecting the extraction of all analytes evaluated (19 organochlorine and organophosphorus pesticides) have been optimised using a multivariate analysis consisting in two steps: a Plackett-Burman design followed by a central composite design (CCD). Seven experimental factors have been evaluated: (i) sample volume; (ii) NADES volume; (iii) sample pH; (iv) extraction time; (v) centrifugation time; (vi) centrifugation speed; and (vii) ionic strength (NaCl %, w v−1). For the significant variables, the optimum values were 10 mL sample and 45 μL NADES. No pH adjustment as well as addition of NaCl were needed. The other variables were set at 3 min extraction time, 5 min centrifugation time and 900×g centrifugation speed, respectively. Under the optimised extraction conditions, the limit of quantification (LOQ) values ranged between 0.2 and 78 ng L−1 for all analysed pesticides. Furthermore, the proposed analytical method has been successfully applied to drinking water (bottled spring water). The recovery study (n = 3) has been evaluated at 0.1, 1.0 and 5.0 μg L−1 spiking levels, obtaining relative recovery values within the range of 70 % and 117 % and RSD values between 1 % and 20 % for all the analytes studied, except for p,p-DDT (56–77 % in high conductivity water samples).

Electron beam irradiation coupled ultrasound-assisted natural deep eutectic solvents extraction: A green and efficient extraction strategy for proanthocyanidin from walnut green husk

Proanthocyanidin (PAC) is recognized as a potent natural antioxidant that prevents various diseases. As societal awareness increases, eco-friendly and efficient natural product extraction technologies are gaining more attention. In this study, an electron beam irradiation (EBI) coupled with ultrasound-assisted natural deep eutectic solvents (NADES) extraction method was developed to enable the green and highly efficient extraction of PAC from walnut green husk (WGH). NADES, prepared with choline chloride and ethylene glycol, demonstrated excellent extraction capacity and storage stability for PAC. Molecular dynamics simulations elucidated the high compatibility between NADES and PAC, attributed mainly to a higher SASA value (207.85 nm2), a greater number of hydrogen bonds (330.99), an extended hydrogen bonding lifetime (4.54 ps), and lower inter-molecular interaction energy. Based on these findings, the optimal conditions (13 kGy EBI, 42 mL/g liquid-solid ratio, 38 °C extraction temperature, 70 min extraction time) resulted in a maximum PAC extraction yield of 56.34 mg/g. Notably, this yield was 32.93 % higher than that observed in samples not treated with EBI and ultrasound-assisted extraction (UAE). Analysis of tissue morphology, extract functional groups and thermal behavior suggested a possible mechanism for the synergistically enhanced PAC extraction by the EBI-NADES-UAE method. Additionally, the PAC extracted using the NADES by the EBI coupled with ultrasound-assisted method exhibited outstanding antioxidant activity (comparable to Vc), digestive enzyme inhibition (IC50: 17–0.61 mg/mL), and anti-glycation capacity (IC50: 86.49 μg/mL). Overall, this work provided a green and efficient strategy for PAC extraction from WGH, elucidated the extraction mechanism and bioactivities, and offered valuable insights for potential industrial applications.

Development of a process for extracting rare earth elements from phosphogypsum by dissolving them in deep-eutectic solvent

ABSTRACT The exploitation of deep-eutectic solvents (DESs) offered a potential substitute for water-based solutions when it came to leaching REEs from phosphogypsum (PG). The REEs leachate from PG obtained by DES which comprised choline chloride (ChCl) and diglycolic acid (DGA) or lactic acid (LA). The data offered a successful method for REEs leaching by DES ChCl:LA. The optimal leaching conditions included 150 min, 70°C, ChCl:LA 1:2 molar ratio of 800 rpm, a 15:1 L/S ratio, and 15% additional water. Cyanex 923 was used to assess REEs extraction from DES leachate. The best parameters were 900 rpm, 0.8 M Cyanex/toluene at 25°C, with 15 min extraction time and 1/1 A/O ratio. The REEs were stripped from the REEs/Cyanex after equilibrating for 20 minutes using 0.7 M citric acid, 1/2 A/O ratio, and 1000 rpm at 25°C. The RE ions precipitated from the obtained stripping solution by 30% oxalic acid and pH 1. The RE oxalate was precipitated and then burned at 700°C to gain RE oxide. The RE oxide powder was subjected to analysis to determine the REEs; it was possible to determine that the REEs were 80.98% in RE oxide concentrate, resulting in a purity of 94.76%.

Enhancement of ultrasonic-assisted and agitation-assisted flaxseed oil extractions: Kinetic modeling and optimization

The flaxseed oil extraction process has been investigated and enhanced by applying Ultrasonic-Assisted Extraction (UAE), and Mixer-Assisted Extraction (MAE) methods in this study. Response Surface Methodology (RSM) was also utilized to find the optimal values of the extraction parameters. The optimal conditions for UAE were determined to be 42.7 min extraction time, 0.283 mm particle size, and 2.48 W/mL power intensity, achieving the oil yield of 94.56 %. For MAE, the optimal conditions were 54.5 min extraction time, 0.274 mm particle size, 21.1 °C temperature, and 0.74 W/mL agitation intensity, resulting in the oil yield of 96.73 %. RSM showed high reliability with R2 values above 0.99 for both methods. Gas Chromatography Flame Ionization Detector (GC-FID) analysis revealed that UAE and MAE resulted in higher unsaturated fatty acids concentrations (85.8 % and 86 %, respectively) compared to CPE (78.2 %). Kinetic studies indicated that UAE and MAE are more efficient in extracting unsaturated fatty acids, with MAE showing the highest double bond content. The Kinetic models demonstrated that extraction kinetics were controlled primarily by solid-phase resistance, with the washing coefficients being significantly higher than the diffusion coefficients. The Perez model calculated diffusion coefficients of 8.03 × 10⁻12 m2/s for UAE and 1.41 × 10⁻11 m2/s for MAE, activation energies of 16.44 kJ/mol for UAE and 15.64 kJ/mol for MAE.

Nectarine core-derived magnetite biochar for ultrasound-assisted preconcentration of polycyclic aromatic hydrocarbons (PAHs) in tomato paste: A cost-effective and sustainable approach

A novel ultrasound-assisted magnetic solid-phase extraction coupled with gas chromatography–mass spectrometry (US-MSPE-GC/MS) was developed to detect trace amounts of polycyclic aromatic hydrocarbons (PAHs) in tomato paste, using a magnetic biochar adsorbent derived from nectarine cores. The highest extraction recovery was attained under 10 mg adsorbent mass, 30 min extraction time, 9 % (w/v) sodium chloride, and elution with 200 μL of dichloromethane. Under optimum conditions, the method demonstrated excellent linearity (R2 > 0.992) across a wide concentration range (0.01-100 ng g−1) with high sensitivity (LODs: 0.028-0.053 ng g−1, LOQs: 0.094-0.176 ng g−1) and good repeatability (RSDs <5.96 %). The application of the US-MSPE-GC/MS method was tested on four brands of real tomato paste and no PAHs were detected in unspiked samples, indicating no background contamination. This method showed high relative recoveries 88.03-98.52 %) and good reproducibility (<9.19 %.) at two concentration levels, confirming its effectiveness for PAH analysis in real samples.

Theoretical exploration and experimental investigation of acidic deep eutectic solvents as green solvents to separate carbazole from model crude anthracene oil

Carbazole is a highly value-added compound derived from crude anthracene. Deep eutectic solvents (DESs) have been extensively used as promising green solvents for the separation of target compounds from mixture. In this work, 135 acidic DESs, formed by combining 9 quaternary ammonium salts with 15 common acids, were screened based on the conductor-like screening model for real solvents (COSMO-RS) model. The four top-ranked DESs, including tetraethylammonium chloride (TEAC)/2 acetic acid (AA), TEAC/2 propionic acid (PA), tetrapropylammonium chloride (TPAC)/2AA, and TPAC/2PA were identified as suitable extractants for carbazole separation based on their performance index (PI). Among them, TEAC/2AA had the best separation performance for carbazole due to its highest PI value (1448.19). Liquid–liquid extraction experiments were performed, and the results were in good accordance with those predicted by COSMO-RS calculations. Therefore, TEAC/2AA was determined as the most promising DES for the carbazole separation. The extraction conditions of TEAC/2AA were optimized. The extraction efficiency (EE) and selectivity (SE) of carbazole were 97.67 % and 66.64 %, respectively, under the optimal extraction conditions (25 ℃, 5 min of extraction time, DES-oil mass ratio of 1:5, initial carbazole concentration of 5000 ppm). Additionally, the deionized water was employed to regenerate the DES and recovery of carbazole. The recycling performance of promising DES was also investigated. Finally, quantum chemical calculations and Fourier Transform Infrared spectroscopy (FT-IR) analysis were conducted to explore the separation mechanism. The results indicated that the separation of carbazole by DESs were predominantly dominated by hydrogen bonds and van der Waals interaction. This work provided a valuable reference for the selection and design of DES extractants to separate other high value-added components from mixture.

Polysaccharides from Hericium erinaceus and its immunomodulatory effects on RAW 264.7 macrophages

This study aimed to optimize the extraction of Hericium erinaceus polysaccharides (HEP) using ultrasound-assisted enzymatic extraction combined with Plackett-Burman design (PBD) and response surface methodology (RSM). The optimal extraction conditions were identified as: 33 min extraction time, 30:1 liquid to material ratio, 38 °C extraction temperature, 9 g/kg cellulase amount, pH 4, and 20 % ethanol concentration. Under these conditions, the extraction yield of HEP was 5.87 ± 0.16 %, consistent with the predicted results. Additionally, the potential immunomodulatory activity of HEP on RAW 264.7 macrophage was evaluated. The results revealed that HEP improved the immunostimulatory activity of RAW264.7 cells, evident from increased production of IL-6 and TNF-α. These findings suggest that HEP is capable of enhancing the immune activity of RAW 264.7 macrophage.

Characterization of Marigold Flower (Tagetes erecta) Extracts and Microcapsules: Ultrasound-Assisted Extraction and Subsequent Microencapsulation by Spray Drying.

Ultrasound-assisted extraction using response surface methodology was employed to extract marigold flower, resulting in a marigold flower extract (MFE) with elevated levels of total phenolic compounds (TPCs), total flavonoid content (TFC), total carotenoid content (TCC), and antioxidant activity, as assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays, under conditions of 40 °C temperature, 15 min extraction time, and 68% ethanol concentration. The MFE was subsequently encapsulated using spray drying with 45% maltodextrin (MD) (MFE-MD; 1:1, 1:2) and 20% gum arabic (GA) (MFE-GA; 1:2, 1:3). The MD (1:2 ratio) sample showed the highest encapsulation yield, while the 45% MD (1:1 ratio) sample exhibited the highest encapsulation efficiency (p ≤ 0.05). Samples containing 45% MD (1:1 ratio) and 20% GA (1:2 ratio) had the highest moisture content, with the 45% MD (1:1 ratio) sample showing the lowest water activity (p > 0.05). These samples also displayed higher L* and a* values compared to the 20% GA samples, which had increased b* values (p ≤ 0.05). Micrographs of the 20% GA (1:3 ratio) and 45% MD (1:2 ratio) samples revealed spherical shapes with smooth surfaces. The 20% GA (1:2 ratio) microcapsules exhibited the highest total phenolic content (TPC) among the samples (p ≤ 0.05). Thus, ultrasound-enhanced extraction combined with response surface methodology proved effective in producing functional food ingredients from plants.

Optimizing Ultrasonic‐Assisted Extraction for Enhanced Yield of Phenolic Compounds and Antioxidant Activity in Cold Brew Coffee

ABSTRACTThis study aimed to identify the best conditions for ultrasonic‐assisted extraction (UAE) of DPPH and phenolic compounds from cold brew coffee using Box‐Behnken Design (BBD) and Response Surface Methodology (RSM). It explored the effects of solid‐liquid ratio (5%–15%), extraction time (40–50 min), and ultrasonic power (70%–80%) on these compounds, finding that all factors significantly influenced the outcomes. Statistical analysis showed the data fit a quadratic polynomial model well, with R2 values of 0.9981 for phenolic compounds and 0.9799 for DPPH radicals. Using 3D surface and contour plots from these models, the optimal extraction conditions for these compounds from cold brew coffee were determined. The highest yields of total phenolic compounds and DPPH radical were obtained when samples were extracted at a 10% solid–liquid ratio, 45 min extraction time, and 75% ultrasonic power. Under these optimal conditions, total phenolic compounds and DPPH radical were 64.10 ± 0.31 gGAE/mL and 61.90 ± 0.14%, respectively, with a maximum caffeine content of 213.13 ± 0.23 mg/L. Gamma irradiation at kGy also reduced cold brew coffee's microbial content. This research may be a new alternative for producing cold brew coffee that will save time and help extend the shelf life.

Green and efficient extraction process of flavonoids from sea buckthorn fruits by natural deep eutectic solvents aided with ultrasound

In recent years, with the advantages of environment friendly, natural deep eutectic solvents (NADES) have been gradually applied for the extracting bioactive compounds from natural products. In this study, NADES-12 (betaine-lactic acid, n/n, 1:2) was selected for the first time to extract flavonoids from sea buckthorn fruit after screening of 23 NADES. The optimal technical conditions of NADES combined with ultrasonic assisted extraction (UAE) were determined by single factor test and response surface optimization test. The results showed that the optimal process for the extraction of antioxidant components by NADES-12 was 37 min extraction time, 40 °C extraction temperature, 17 % (v/v) water content, 40 mL mg−1 liquid–solid ratio, 160 W ultrasonic power, and a maximum extraction rate of 5.248 mg g−1, and the extraction yield is 2.4 and 3.1 times that of 60 % ethanol and water. The sea buckthorn extract by NADES has antioxidant activity. The study proposes a green and efficient method for the extraction of bioactive components from sea buckthorn fruit and provides a valuable basis for the exploitation of other natural plants.

Optimization of Ultrasonic-Assisted Extraction Condition for Total Phenolic Compounds from Citrus Hystrix Peel

This study aims to optimize the extraction conditions of Ultrasonic-Assisted Extraction (UAE) on the extraction of phenolic compound and antioxidant activity of Citrus Hystrix (C. Hystrix) peel. The experimental design was carried out using Face Centered Central Composite Design. A total of 20 sets of different combination experiment designs were generated from 3 parameters; temperature (30-50°C), time (20-40 min), and raw material to the solvent ratio (1:30-1:70). The highest yield of total phenolic compounds was 4.36 mg/g with the optimize extraction condition of , 30 min extraction time and 1:50 raw material to the solvent ratio. The result of this study indicates that the phenolic compounds extracted from C. Hystrix peel could have the potential to be used for pharmaceutical and food applications.