Purpose: This study aimed to assess the relationship between work-life balance, professionalism, the nursing work environment, job satisfaction, and turnover intention, and to identify the influencing factors on turnover intention among millennial nurses at rural general hospitals. Method: Participants were 154 nurses born after 1989, working in two general hospitals located in C city of G province. Data were collected from August 25 to September 6, 2023, using a self-report questionnaire. Descriptive statistics, independent t-tests, one-way ANOVA, Pearson correlation coefficients, and hierarchical regression analyses were conducted using SPSS version 25.0. Results: In the hierarchical regression analysis, the variables affecting turnover intention were years of total experience, with different impacts noted for those with less than 1 to 5 years (β=.34, p=.002) and more than 5 years (β=.38, p=.001) in Model 1. In Model 2, work-life balance (β=-.19, p=.019) and professionalism (β=-.26, p=.001) were significant variables affecting turnover intention. Model 3 showed that the nursing work environment was an additional significant variable affecting turnover intention (β=-.35, p<.001), but job satisfaction (β =-.06, p=.507) did not significantly affect turnover intention. The total explanatory power was 34.9% (F=9.73, p<.001, R²=.349). Conclusion: The results suggest that various nursing management strategies and career management programs that consider nurses’ clinical experience and the nursing work environment are needed to reduce nurses’ turnover intention.