Milled Rice Research Articles

Quantitative trait locus mapping and OsFLOq12 identification for rice grain hardness: towards improved rice flour for wheat substitution.

Recent shifts in consumer dietary preferences have led to a significant decline in rice consumption in Korea, resulting in surplus rice production. To address this issue, rice flour has been proposed as a substitute for wheat flour. However, the physical, chemical and structural differences between rice and wheat, particularly in grain hardness, pose challenges in using rice flour as an alternative. Understanding the genetic factors that influence rice grain hardness is crucial for improving the milling process and producing high-quality rice flour suitable for wheat flour substitution. In this study, various grain traits, including length, width, thickness, length-to-width ratio and hardness, were measured in a population of brown and milled rice. Quantitative trait locus (QTL) analysis revealed a significant association between grain hardness and thickness, with QTLs for grain hardness mapped on chromosomes 1 and 12 for brown and milled rice, respectively. A total of 20 candidate genes related to grain hardness were identified through QTL analysis. Among them, OsFLOq12 (LOC_Os12g43550) was identified as a key gene influencing grain hardness, which encodes a Ras small GTPase. Phenotypic analysis showed differences in endosperm appearance and particle size between lines with low and high grain hardness. The genetic analysis of OsFLOq12 revealed a single nucleotide polymorphism associated with grain hardness. This study provides valuable insights into the genetic background of grain hardness, offering a foundation for breeding rice varieties optimized for flour production as a viable substitute for wheat flour. © 2024 Society of Chemical Industry.

Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of qMLWR11.1 and qMLWR11.2

Grain shape in rice (Oryza sativa L.) is a complex trait governed by multiple quantitative trait loci (QTLs). To dissect the genetic basis of rice shape, QTL analysis was conducted for milled rice grain width (MGW), milled rice grain length (MGL), and milled rice length-to-width ratio (MLWR) using a recombinant inbred line (RIL) population of F10 and F11 generations derived from a cross between Yuexiangzhan and Shengbasimiao. A high-density genetic map consisting of 2412 bins was constructed by sequencing 184 RILs, spanning a total length of 2376.46 cM. A total of 19 QTLs related to MGL, MGW, and MLWR were detected under two environments. The range of phenotypic variation attributed to individual QTL ranged from 1.67% to 32.08%. Among those, a novel locus for MGL, MGW and MLWR, designated as qMLWR3.2, was pinpointed within a specific ~0.96-Mb region. Two novel loci for MGW and MLWR, qMLWR11.1 and qMLWR11.2, were verified within ~1.22-Mb and ~0.52-Mb regions using three RIL-developed populations, respectively. These findings lay the foundation for further map-based cloning and molecular design breeding in rice.

Effect of Size Reduction Processes on Rice Flour Properties and the Quality of their Bakery Products

Currently, there is an incremental demand for ricebased products among health-conscious consumers in the dynamic food market. The method and the machinery used in the size reduction processes have a crucial impact on the physicochemical properties of rice flour, thereby affecting the quality and consistency of the final products. Consequently, certain starch modification techniques can be implemented to eliminate some baker-unfriendly properties associated with rice flour. This brief review is focusing on literature pertaining to different rice milling techniques, certain physicochemical and functional flour properties, a comparison of rice flour properties relevant to different rice milling techniques, the relationship between different rice flour properties and their effect on the quality of bakery products, and finally the improvement of flour properties by different starch modification techniques.

Optimization of milling degree for maximizing nutrient retention and yield in milled rice: A study on six common Bangladeshi rice cultivars

Awareness of the nutritional value and health benefits of milled rice is crucial for its role as a staple food. This study explores the impact of different degrees of milling (DOM)—5%, 7%, and 9%—on head rice yield, proximate composition (protein, fat, fiber, and carbohydrates), and mineral content (sodium, magnesium, potassium, iron, calcium) in six rice cultivars: BRRI Dhan 28, 29, 36, 90, 51, and Bogura Katari. Our findings reveal that head rice yield decreases significantly, by 3% to 8%, when DOM is increased from 5% to 7% or 9%. Increasing the DOM also led to pronounced losses of protein (15% to 25%), fat (40% to 70%), and fiber (15% to 40%), while carbohydrate content increased. Additionally, substantial reductions in mineral content were observed at 9% DOM. Notably, consuming rice milled at 5% DOM can meet significant portions of daily nutrient requirements: 50% of protein, 10% of fiber, 20% of fat, 10% of sodium, 80% of magnesium, 35% of potassium, 100% of iron, and 30% of calcium, based on a per capita consumption of 400 g per day. This research highlights the importance of optimizing DOM between 5% and 7% to enhance head rice yield and maximize nutrient retention.

Market Concentration of Branded Rice in Kerala

In Kerala rice is a staple diet. As per the information from Kalady Rice Millers’ Consortium, 40 per cent of the consumers in Kerala prefer branded rice, and out of this Kerala absorbs only 16 per cent, the rest of the branded rice is from outside states like Andhra Pradesh, Tamil Nadu and West Bengal. This study investigates the market concentration of Kerala’s branded rice market, focusing on the dominance of a few key players and its market share, pricing power, and concentration. The analysis employs four-firm concentration ratio, the Herfindahl-Hirschman Index (HHI), Gini coefficient and the Lorenz curve to reveal an oligopolistic structure, where a small number of firms control a substantial portion of the market. Major players like Pavizham, Keerthi Nirmal, Periyar, Kottaram, and Jayabharath, alongside the Kalady Rice Miller’s Consortium, play a crucial role in shaping the branded rice market in Kerala. The study involved a survey conducted between November 2023 and June 2024, with a random sample of 10 non-exporters, 5 exporters, and one co-operative rice mill, selected from the 46 branded rice manufacturers in the state, predominantly from Ernakulam and Palakkad districts. The findings of the study highlighted that the branded rice market in Kerala had an oligopolistic behaviour. This research fills a gap in understanding the distribution of market power in Kerala’s rice industry and offers suggestions for fostering greater competition and inclusivity in the market.

Harnessing biomass waste-to-energy for sustainable electricity generation: prospects, viability, and policy implications for low-carbon urban development

AbstractBiomass waste-to-energy (WtE) generation is a potential pathway for green urban transition in developing countries which can contribute significantly to sustainable development goal 7: affordable and clean energy. However, unlike fossil fuel energy systems, the economic returns from WtE systems are low because WtE generation is capital-intensive and requires subsidies. This study examines the prospects of a sustainable biomass electricity generation from rice husk (RH) using a large dataset of rice milling activities in a fast paced urban transition economy. The study analyzes the viability of several RH biomass electricity generation scenarios using indicators such as net electricity output, economic returns (benefits), and levelized cost of electricity (LCOE). The results show that several mills/mill clusters generate sufficient daily RH that can power between 0.8 and 2.2 MW plant with a combined electricity output of about 500,000 MWh per annum. The economic analyses show that all RH biomass electricity generation scenarios return positive economic benefits under reduced social discount rates of 2–6%. Moreover, the LCOE of all scenarios are less than those of electricity generated from other sources. These results demonstrate that biomass waste-to-energy generation is viable for green urban development through low-carbon decentralized energy systems. Several policy implications of the findings are highlighted, including the need for policymakers and energy stakeholders to adopt sustainable biomass energy generation models such as “design, build, and operate” (DBO) to achieve sustainable WtE generation regimes that ensure green urban transition. Such a model will contribute to a circular economy and facilitates sustainable urban development that satisfies climate-related SDGs. Graphic abstract

Determinants of net value added to produced and processed paddy rice in South East, Nigeria

The study principally examined the determinants of net value added to produced and processed paddy rice in South east Nigeria It specifically, identified the socio-economic characteristics of the respondents, analyzed the determinants of net value added to produced and processed paddy rice as well as ascertained the constraints to processed and produced paddy rice. Multi-stage sampling technique involving purposive and random sampling methods was used to select 240 respondents. Primary data were collected on rain-fed production season using structured questionnaires administered to the respondents by personal interview. Descriptive statistics,, multiple regression and principal component analysis were used to analyze the data. Results obtained showed that gender distribution of the respondents was 63%males and 38% females. More respondents are between the ages of 41-50 and31-40 respectively and house hold size of 4-6 .Farming experience showed that 136 respondents representing 57% had engaged in the business between 11-20yrs.The results also showed that 67% of the respondents accessed farm credit while 25% did not.66% of the respondents belonged to farmers association while 75% were not visited by extension agents. Variables (age, gender, years in school, household size, years of experience, training/extension visits, costs incurred, value addition technique and market price) significantly influenced the net value added while marital status, membership of cooperative, and access to credit did not. Constraints to paddy producers and processors were accessibility to farm machine, poor infrastructure and scarcity/high cost of labor, low access to credit/grant; low proactive measures to flood and drought control; insufficient knowledge of agro-chemicals, and late provision of essential training and services; and processors: use of outdated rice milling machines, high cost of modern rice processing machines, low access to modern rice processing machineries and low acceptability of local rice. Recommendations include; establishment of farm machinery hiring centers for producers and supply of modern rice milling machines to rice processing clusters, development of dams and irrigable lands, training and re-training on access to fund, utilization of biodegradable wastes from paddy rice, proactive measures of flood and drainage control, adequate use of agro-chemicals, use of modem techniques, and involvement of National Agency for Food and Drug Administration and Control in quality control. Keywords: Agriculture, Paddy Rice, Value Addition, Nigeria.

Regulation effectivity to sustainable competitive advantage on rice mill industry evidence Indonesia

The purpose of this study is to analyse and prove the impact of regulatory effectiveness on the supply chain and its impact on sustainable competitive advantage for rice milling companies in Indonesia. The research method used was a quantitative analysis, employing a sample of 200 rice milling companies in East Java. The results of this research indicate that regulations do not have a significant effect on the supply chain, Regulations do not have a substantial effect on sustainability Competitive Advantage, Supply chain has a significant impact on sustainable competitive advantage, in addition, flexibility management can moderate the relationship between the supply chain and sustainable competitive advantage, but flexibility management is not able to moderate the regulatory relationship with sustainable competitive advantage.

Stakeholders Analysis in the Development of Local Paddy Varieties in Padang City

The development of local paddy varieties involves many stakeholders. Each stakeholder has a different role ranging from the socialization and farming to the marketing of local rice varieties. This research aims to identify stakeholders and analyze their role in developing local paddy varieties using a descriptive qualitative method. The respondents in this research are stakeholders involved in the development of local paddy varieties in Padang City. The results show that the stakeholders involved are the Padang City Agriculture Office, Agricultural Extension Center, farmers, farmer groups, landowners, seed breeders, production facilities providers, agricultural labor, rice milling units, collecting traders, colleges, sub-district administration, urban village administration, financial institutions, the Regional People’s Representative Council, retailers, and consumers. Classifications of stakeholders show that the Padang City Agriculture Office, the Agricultural Extension Center, farmers, and farmer groups have high influence and interest. These stakeholders are classified as key players. Meanwhile, sub-district administration, urban village administration, financial institutions, and Regional People’s Representative Council have low influence and interest. They are classified as the crowd. All of the stakeholders mentioned that roles play their position as policymakers, coordinators, facilitators, implementers, and accelerators. Keywords: paddy, stakeholders, interest, power, role

The impact of milling degree on physicochemical, nutritional, rheological, textural properties, and in vitro digestibility of rice: From brown rice to white rice

The objective of this study was to characterize the rice milling fractions acquired at each stage of a commercial milling system. This characterization included an analysis of color, ash content, dietary fiber, mineral composition, as well as antinutritional compounds like phytic acid, trypsin inhibitor activity, and saponin. Additionally, we investigated in vitro starch and in vitro protein digestibility, along with pasting, cooking, and textural properties.Our findings revealed that milling improved the visual appeal of rice (e.g., volume expansion, weight gain, whiteness) and notably enhanced its starch digestibility. However, milling reduced dietary fiber, mineral content, antinutrients, cooking time, and texture characteristics such as hardness, chewiness, gumminess, springiness, and cohesiveness to varying degrees. Certain parameters, such as dietary fiber, exhibited a gradual change with the duration of milling, while others, such as mineral content and texture, showed significant variation at the initial stage of milling, particularly at the first mill.

The effects of milling on the chemical forms, bioavailability, and in vivo distribution of Vitamin B1 in rice

Milling not only reduces the content of Vitamin B1 (Vit B1) in rice but may even affect its bioaccessibility, further affecting its activity in vivo. Therefore, this study investigated the changes in Vit B1 content and its chemical forms in rice at different degrees of milling (DOM) before and after cooking, as well as their bioaccessibility in vitro and bioavailability in vivo. In raw unmilled rice, the proportion of bound Vit B1 was slightly lower than that of free Vit B1, and the proportion increased significantly after cooking. DOM< 8% hindered this change of bound Vit B1 after cooking, resulting in a decrease in Vit B1 thermal stability from 90% to 80% and bioaccessibility from 80% to 60%. Fortunately, the content of Vit B1 stabilized in the digestive fluid remained superior to that of the high DOM group. DOM<8% shortened the time for Vit B1 to peak in the blood concentration of mice, whereas the decrease in bioavailability of Vit B1 in blood and liver was insignificant, unlike bioaccessibility. Hence, DOM < 8% can be applied to the rice milling process to maintain nutritional value. Correlation analyses indicated that the bound form of Vit B1 in cooked rice was the main factor affecting its digestion and absorption. These findings provide further support for the development of moderate rice processing.

Industrial Pollution in Patna City : Physico-Chemical Analysis of Effluents and Their Effects on Water and Soil

Industrial pollution is a significant concern in Patna City, with rapid urbanization and industrial growth contributing to the degradation of natural resources. This study focuses on the physico-chemical analysis of industrial effluents and their effects on the water and soil quality in and around Patna. Effluents from various industries, including textile, sugar, rice mills, and small-scale manufacturing units, are released into nearby water bodies and soils, posing serious environmental risks. The research aims to assess the levels of pollutants by analysing key physico-chemical parameters such as pH, total dissolved solids (TDS), biochemical oxygen demand (BOD), chemical oxygen demand (COD), heavy metals (lead, chromium, cadmium), and organic contaminants in industrial effluents. Samples were collected from various locations near industrial zones and water bodies. The study also evaluated the impact of these pollutants on soil and groundwater quality. It was found that prolonged exposure to industrial effluents led to the accumulation of toxic metals in soil, reducing its fertility and altering its structure. Additionally, groundwater sources were contaminated with hazardous chemicals, making them unfit for drinking and irrigation purposes. The implementation of advanced treatment technologies and sustainable industrial practices is crucial for safeguarding the environment and public health in Patna City.

Design Micro-Hydro Power Plant Water Resources System for Small Medium Enterprise Rice Mill in Nagari Kamang Hilia Agam

Processing rice products into rice requires further processing, where the grain harvested from farmers is processed into rice. The Nagari Binaan Community Service team from Padang State University will later develop this process. Farmer groups say that the availability of continuous and cheap electricity is their obstacle in producing good and cheap rice products. Counseling and assistance in making civil buildings capture turbulence water micro-hydro power plants is a solution to partner problems offered by the community service team of the Nagari program assisted by Padang State University. The output of the Nagari Fostered Program will be used to build a 5000-watt capacity power plant, supporting the need for free and environmentally friendly electricity. This aligns with the government's renewable energy program for 2030, which aims to produce 25% of the national electricity from renewable sources. This program is planned for 3 (three) stages, where the first year is mapping and detailed engineering design in the form of reports and design drawings. The second year is in the form of civil construction, and the third year's target is the installation of electrical machines and panels for commissioning. There will be training for partners, such as farmers in Kanagarian Kamang Hilia, Agam.

Starch lysophospholipids contents affect storage quality of paddy rice

Starch Lysophospholipids (LPLs) have a significant effect on rice grain quality, but how starch LPLs affect paddy rice storage quality is barely understood. The dynamic changes in storage quality indicators of a japonica rice (XS110) and its low LPLs (XS110-J1) and high LPLs (XS110-J2) mutants were investigated. The fatty acid values (FAV) of J1 were significantly higher than those of XS110 and J2 after one year of storage under 24 and 37 °C. Although FAV rose gradually during storage time, it increased relatively more at 37 °C than 24 °C. The malondialdehyde (MDA) content increased significantly in the early stage and decreased gradually during later storage. The MDA content of rice stored at 24 °C was significantly higher than that at 37 °C. The catalase (CAT) activity exhibited a decreasing trend during storage and was relatively higher at 24 °C than 37 °C. The CAT of J2 was significantly higher than those of XS110 and J1. Total lysophospholipid content was relatively stable although some individual LPLs changed significantly during storage. The fatty acid component content in the milled rice decreased during storage, and the content at 37 °C was lower than that at 24 °C. In conclusion, paddy rice with low starch LPLs content is more susceptible to deterioration during storage, suggesting that high starch LPLs can better maintain rice quality and prolong storage time during storage of paddy rice.

PROFITABILITY ANALYSIS OF RICE MILLING AND MARKETING IN BAUCHI STATE, NIGERIA

The study analysed the profitability of rice milling and marketing in Bauchi State, Nigeria. Multi-stage sampling procedure was used for the selection of respondents. The respondents comprised rice processors (millers) and marketers. Data were collected using questionnaire and analysed using descriptive statistics, farm budgeting techniques and multiple regression analysis. The findings revealed that majority (87.5%) of the millers and all (100%) of marketers were male and married with a mean age of 39 and 44 years respectively. However, 43.8% of the millers and 40% of the marketers had a family size of between 6-10 people per household with majority (62.5%) of the millers having tertiary education. The analysis also shows that half (50%) and more than half (53%) of the millers and marketers had a marketing and milling experience of between 11-20 years. The result of the budgeting model revealed that millers had a net income of N714 per bag with a return per naira of N1.46 and marketers had a net income of N1, 672 per bag with a return per naira of N 0.12. The results of the regression analysis revealed that years of milling experience and education level were the factors influencing rice milling while household size and membership of association were significant factors influencing rice marketers’ income. However, high cost of diesel (96.7%) and transportation cost (95%) were the major constraints faced by the millers and marketers in the study area. The study concluded that, milling and marketing of rice is a profitable ventures in the study area. It is therefore, recommended that government together with stakeholders should provide adequate infrastructure and introduce favorable policies to strengthen the rice value chain in the study area.

Physicochemical and Morphological Changes in Long-Grain Brown Rice Milling: A Study Using Image Visualization Technologies

This study evaluated the changes in physicochemical properties and appearance quality of long-grain rice during the grinding process using image technologies and aimed to provide reference for future research. The brown rice milling process was divided into three stages, and flatbed scanning, scanning electron microscopy (SEM), X-ray micro-computed tomography (micro-CT), low-field nuclear magic resonance (LF-NMR), and headspace–gas chromatography–ion mobility spectrometry (HS–GC–IMS) were employed to examine the physicochemical and volatile properties of the samples. Results revealed a continuous increase in the degree of milling, with a broken rice rate and a whiteness value increasing by 50.84% and 21.13%, respectively, compared with those during the initial stage; dietary fiber and vitamin B1 contents were reduced by 54.41% and 66.67%, respectively. The image results visualized showed that the cortex of brown rice was gradually peeled off with the increase in milling degree; the cortical thickness was gradually reduced, the endosperm was gradually exposed, and the surface was smoother and shinier. T2 populations exhibited a shift toward longer relaxation times, followed by a decrease in relaxation time during the milling process. Additionally, 31 target compounds impacting rice flavor, mainly ketones, alcohols, and esters, were identified, and the concentration of volatile substances in the B region decreased with the reduction in the bran layer; the concentration of volatile substances in the C region provided rice flavor, which increased with the milling process. This study showed changes in the physicochemical properties and appearance quality of long-grain brown rice during milling. Furthermore, the use of various image processing techniques offers significant insights for optimizing processing parameters and enhancing overall quality and taste.

Specialized Metabolites from the Husks of Rice Oryza Sativa L. and their Biological Activities.

Rice (Oryza sativa L.) husk harbors a substantial proportion of biological metabolites, as one of the most plentiful agriculture by-products in rice milling process, rice husk remains poorly utilized. As a continuing search for potential bioactive molecules from the husk of rice, a totally of twelve components (1-12), including six sterol ferulates (1-6), one flavonoid (7), one dipeptide (8), and four phenylpropanoid derivatives (9-12) were obtained. All the chemical structures were elucidated based on comprehensive spectroscopic data. Wherein, compounds 1 and 2 were yield as previous undescribed metabolites, and the comprehensive NMR data for compounds 3 and 4 were first presented in its entirety. Motivated by the similarity of the structural motifs of components 1-6 to that of reported sterol ferulates, the antioxidant and anti-inflammatory effects for compounds 1-6 were evaluated in vitro. Among them, compounds 5/6 had a significant antioxidant activity compare to that of vitamin E in both DPPH and reducing power assay up to the concentration 40 μg/ml; while compounds 1 and 2 exhibited weak suppressive effect on the production of nitric oxide, with the IC50 values of 53.27±1.37 μM.

Sintesis Zeolit dari Abu Sekam Padi (OryzaSativa L.) Serta Uji Efektifitasnya Sebagai Material Antiseptik

Rice husks are obtained from 20% rice milling and have a silica content of 94-99% of ashed rice husks. Zeolite is a silica-based material and is known as an aluminosilicate compound, because it is formed from two main components, namely tetrahedral AlO4- and SiO4¬-. Synthetic zeolite is obtained by reacting Sodium Silicate (A) with Sodium Aluminate (B) which is then aged at 100oC for 24 hours to produce white synthetic Zeolite powder. Zeolite synthesis will be made with three variations of mixture ratios, namely 30A:70B, 50A:50B, 70A:30B and the characteristics of each will be evaluated. To test the success of this synthetic zeolite as an antiseptic material, its ability to reduce the infectious value of Bacteriuria and Candiduria will be tested, which will be represented by Escherecia coli and Candida albicans. The results obtained from the three variations of synthetic zeolite that were made had the same characteristics and had a ratio of less than two or type A. Synthetic zeolite made at a dose of 600 mg had the ability to act as an antiseptic material against Escherichia coli 104 by reducing the number of colonies to 72-79 %, as well as Candida albicans 103 was able to reduce the number of C. albicans colonies by 74-80%, this value is lower than Attapulgite 600mg in reducing the same number of colonies, namely 90% for bacteriuria and 94.90% for candiduria. To equalize its capabilities with Attapulgite 600 mg, the dosage of the synthetic zeolite obtained in the research was increased to 700 mg

Predictive modeling of rice milling degree for three typical Chinese rice varieties using interpretative machine learning methods.

Brown rice over-milling causes high economic and nutrient loss. The rice degree of milling (DOM) detection and prediction remain a challenge for moderate processing. In this study, a self-established grain image acquisition platform was built. Degree of bran layer remaining (DOR) datasets is established with image capturing and processing (grain color, texture, and shape features extraction). The mapping relationship between DOR and the DOM is in-depth analyzed. Rice grain DOR typical machine learning and deep learning prediction models are established. The results indicate that the optimized Catboost model can be established with cross-validation and grid search method, with the best accuracy improving from 84.28% to 91.24%, achieving precision 91.31%, recall 90.89%, and F1-score 91.07%. Shapley additive explanations analysis indicates that color, texture, and shape feature affect Catboost prediction accuracy, the feature importance: color>texture>shape. The YCbCr-Cb_ske and GLCM-Contrast features make the most significant contribution to rice milling quality prediction. The feature importance provides theoretical and practical guidancefor grain DOM prediction model. PRACTICAL APPLICATION: Rice milling degree prediction and detection are valuable for rice milling process in practical application. In this paper, image processing and machine learning methods provide an automated, nondestructive, and cost-effective way to predict the quality of rice. The study may serve as a valuable reference for improving rice milling methods, retaining rice nutrition, and reducing broken rice yield.

Potential antimicrobial and fruit juice clarification activity of amylase enzyme from Bacillus strains

The hydrolytic enzyme, amylase possesses wide industrial applications and its production from bacterial sources by submerged fermentation is much simplified and economical. The research aimed to characterize amylase-producing bacteria and evaluate their potential for amylase activity regarding antimicrobial and fruit juice clarification. In current study, Bacillus licheniformis, Bacillus amyloliquifaciens, Bacillus cereus, Bacillus subtilis and Bacillus paramycoides was identified by 16S rRNA sequencing. After submerged fermentation, amylase activity of bacteria was measured by 3, 5-dinitro salicylic acid (DNS) assay. A substantial amount of amylase (423.47 mg/ml) in crude extract was measured by Bradford protein assay. Later, ammonium sulfate (80 %) precipitated partially purified amylase showed 1.6 times enhanced amylase activity (1484.94 U/ml) compared to crude amylase (973.23 U/ml). For highest amylase production, 72 h of optimum fermentation period was recorded at pH 7 with 2 % starch as substrate. Potent thermophilic amylase activity was observed at 65 °C. In apple juice clarification activity of amylase, turbidity of juice was reduced to 54.18 %. Potential antimicrobial property of amylase was detected with largest zone of inhibition against Escherichia coli ATCC 25922 (22.36 mm) and Mucor sp. ATCC 48559 (22.45 mm). Considering promising amylase properties, amylase-producing Bacillus strains from rice mill soil can be fermented for large scale amylase production providing application for industrial purposes including fruit juice clarification and antimicrobial activities. It will also overthrow the requirement of employing expensive and harmful chemicals in fruit juice clarification and combating pathogens.