The present study was aimed atmeasuring the concentration ofaflatoxin M1 (AFM1) inthe milk ofHolstein Friesian cows, its effect onthe milk quality and seasonal trends, aswell asto investigate the efficacyofacommercial clay-based toxin binder. For this purpose, milk samples from dairy cows (n = 72) were collected and assayed for AFM1 before employing aclay-based toxin binder. The milk samples (n = 72) were collected from selected animals, revealing that 69.4% ofthe milk samples had AFM1 levels above the United States permissible limit (0.5 μg/kg). Theincidence ofAFM1 inmilk during the winter and summer was 82.5% and 53.1%, respectively. Owing tothepresence ofAFM1, the level ofmilk fat, solids-not-fat, and protein were found tobelow. Subsequently, theaffected animals were divided into two groups, i.e., AFM1 positive control (n = 10) and theexperimental group (n = 40). The experimental group ofanimals were fed the clay-based toxin binder at25 g/animal/day. Aprogressive decrease of19.8% inthe AFM1 levels was observed onday4 and onday7 (53.6%) inthe treatment group. Furthermore, thefat, solids-non-fat and protein increased significantly inthe milk. Inconclusion, ahigh level ofAFM1 contamination occurs inthe milk inPakistan, affecting the quality ofthe milk production. Clay-based toxin binders may beused toensure the milk quality and toprotect the animal and consumer health.